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    • 9. 发明公开
    • HIGH RESOLUTION GENOME SCANNING
    • 高分辨率基因组扫描
    • EP1114188A2
    • 2001-07-11
    • EP99969451.6
    • 1999-09-17
    • University of Nebraska-Lincoln
    • BENSON, Andrew K.,University of Nebraska-Lincoln
    • C12Q1/68
    • C12Q1/6858C12Q1/6827C12Q2527/137C12Q2531/113
    • A method is disclosed for the detection of polymorphisms in a DNA sequence on nucleotide differences. DNA sequences from different sources are amplified by the polymerase chain reaction using primers based on strand biased, over represented oligonucleotide sequences and differences in the nucleotide sequences of the amplification products determined. Also disclosed are methods for the identification of individuals or the species, strain or serotype of an organism using the method of the present invention. In addition, kits are disclosed for the determination of polymorphisms using the method of the present invention. The ability to determine genetic polymorphisms has widespread application in areas such as genome mapping, genetic linkage studies, medical diagnosis, epidemiological studies, forensics and agriculture.
    • 公开了一种检测核苷酸差异的DNA序列多态性的方法。 使用基于链偏向的,过度表现的寡核苷酸序列和所确定的扩增产物的核苷酸序列差异的引物,通过聚合酶链式反应扩增来自不同来源的DNA序列。 还公开了使用本发明的方法鉴定个体或生物体的物种,菌株或血清型的方法。 另外,公开了使用本发明的方法测定多态性的试剂盒。 确定遗传多态性的能力在诸如基因组作图,遗传连锁研究,医学诊断,流行病学研究,法医学和农业等领域具有广泛的应用。