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81. 发明公开

EP2593568A2 NANOPORE-FACILITATED SINGLE MOLECULE DETECTION OF NUCLEIC ACIDS 有权转让
标题翻译：纳米多孔负载NUCLEIC的单分子检测
公开(公告)号：EP2593568A2
公开(公告)日：2013-05-22
申请号：EP11807539.9
申请日：2011-07-14
申请人： The Curators Of The University Of Missouri
发明人： GU, Li-qun , WANG, Yong , TIAN, Kai
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6825 , C12Q1/6886 , C12Q2600/178 , G01N33/48721 , C12Q2523/307 , C12Q2525/161 , C12Q2525/207 , C12Q2565/631
摘要： The present invention provides a new and improved oligonucleotide detection method based on the nanopore technology with a probe containing a complementary sequence to the target oligonucleotide and a terminal extension at the probe's 3' terminus, 5' terminus, or both termini. The improved nanopore sensor with the probe enables sensitive, selective, and direct detection, differentiation and quantification of target oligonucleotides such as miRNAs. The inventive detection method may also be employed as a non-invasive and cost-effective diagnostic method for cancer detection based on miRNA levels in the patient's blood sample.

82. 发明公开

EP2572004A2 DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY PTO CLEAVAGE AND EXTENSION ASSAY 有权
标题翻译： PTO切割和扩展测定法检测靶核酸序列
公开(公告)号：EP2572004A2
公开(公告)日：2013-03-27
申请号：EP12733915.8
申请日：2012-01-11
申请人： Seegene, Inc.
发明人： CHUN, Jong Yoon , LEE, Young Jo
IPC分类号： C12Q1/68 , C12Q1/48 , C12N15/11
CPC分类号： C12Q1/6837 , C12Q1/6818 , C12Q1/683 , C12Q1/6851 , C12Q1/6853 , C12Q1/6858 , C12Q1/6869 , C12Q2525/161 , C12Q2527/107 , C12Q2561/109 , C12Q2565/1015 , C12Q2561/113 , C12Q2561/101
摘要： The present invention relates to the detection of a target nucleic acid sequence by a PTOCE (PTO Cleavage and Extension) assay. The present invention detects a target nucleic acid sequence in which the PTO (Probing and Tagging Oligonucleotide) hybridized with the target nucleic acid sequence is cleaved to release a fragment and the fragment is hybridized with the CTO (Capturing and Templating Oligonucleotide) to form an extended duplex, followed by detecting the presence of the extended duplex. The extended duplex provides signals (generation, increase, extinguishment or decrease of signals) from labels indicating the presence of the extended duplex and has adjustable T
m value, which are well adoptable for detection of the presence of the target nucleic acid sequence.
摘要翻译：本发明涉及通过PTOCE（PTO切割和延伸）测定来检测靶核酸序列。本发明检测靶核酸序列，其中与靶核酸序列杂交的PTO（探针和标签寡核苷酸）被切割以释放片段，并且该片段与CTO（捕获和模板寡核苷酸）杂交以形成延伸然后检测是否存在扩展双工。扩展双链体从标签中提供指示扩展双链体存在的信号（产生，增加，消除或减少信号），并且具有可调整的T m值，这对于检测靶核酸序列的存在是很好的选择。

83. 发明公开

EP2553124A1 A METHOD OF REGULATING OLIGONUCLEOTIDE FUNCTIONALITY 审中-公开
标题翻译：一种调节寡核苷酸功能的方法
公开(公告)号：EP2553124A1
公开(公告)日：2013-02-06
申请号：EP11761814.0
申请日：2011-02-25
申请人： Monoquant Pty Ltd , Flinders University
发明人： MORLEY, Alexander, Alan , BRISCO, Michael, Julian
IPC分类号： C12Q1/68
CPC分类号： C12P19/34 , C12N15/10 , C12Q1/6844 , C12Q1/686 , C12Q2525/161 , C12Q2525/186 , C12Q2527/107 , C12Q2549/119
摘要： The present invention relates generally to a method of regulating oligonucleotide functionality and, more particularly, to a method of regulating the functionality of a primer or probe. The method of the invention is designed to provide a means to selectively inactivate or activate the functionality of an oligonucleotide, such as a primer, thereby providing means to regulate the progress of any method using that oligonucleotide. The development of a means to regulate the functionality of an oligonucleotide, such as a primer, is useful in a range of applications including, but not limited to, amplification reactions such as PCR, isothermal amplification and nucleic acid strand extension. With respect to amplification reactions, these have wide utility including the diagnosis and/or monitoring of disease conditions which are characterised by specific gene sequences and the characterisation or analysis of specific gene regions of interest.
摘要翻译：本发明一般涉及调节寡核苷酸功能的方法，并且更具体地涉及调节引物或探针的功能的方法。设计本发明的方法以提供选择性失活或激活寡核苷酸（例如引物）的功能性的手段，由此提供调节使用该寡核苷酸的任何方法的进展的手段。调节寡核苷酸（例如引物）功能的手段的开发可用于一系列应用，包括但不限于扩增反应如PCR，等温扩增和核酸链延伸。就扩增反应而言，这些方法具有广泛的实用性，包括诊断和/或监测以特定基因序列为特征的疾病状况以及特定目的基因区域的表征或分析。

84. 发明公开

EP2529026A1 SELF-FOLDING AMPLIFICATION OF TARGET NUCLEIC ACID 有权
标题翻译：扩增SELBSTFALTENDE靶核酸
公开(公告)号：EP2529026A1
公开(公告)日：2012-12-05
申请号：EP11701445.6
申请日：2011-01-25
申请人： Rd Biosciences Inc.
发明人： JU, Jingliang
IPC分类号： C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/6853 , C12N15/1096 , C12Q1/6844 , C12Q1/6865 , C12Q1/707 , C12Q2531/143 , C12Q2525/301 , C12Q2525/161 , C12Q2525/143
摘要： The application relates generally to methods useful for the selective amplification of one or more target nucleic acid or fragments thereof, as well as compositions and kits comprising said amplification reaction mixtures. More specifically, the application relates to a composite primer that comprises a 5 ' promoter portion and a 3 ' target-recognition portion which is complementary to the 3 ' end portion of a target polynucleotide sequence; and optionally, a means for identifying the 5' end portion of the target polynucleotide sequence. The amplification reaction mixture comprises at least one handle-stem-loop structure which comprises a 5 ' single-stranded handle comprising the promoter portion and a double-stranded stem comprising at least one pair of self- folding segments hybridized to each other, and optionally, a single-stranded loop comprising the sequence between the pair of self- folding segments.

85. 发明公开

EP2518162A1 Multitag sequencing and ecogenomics analysis 有权
标题翻译： Mehrfachetiketten-Ablaufsteuerung undökonome分析
公开(公告)号：EP2518162A1
公开(公告)日：2012-10-31
申请号：EP12161878.9
申请日：2007-11-15
申请人： Biospherex LLC
发明人： Gillevet, Patrick M.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/6858 , C12Q2600/156 , C12Q2600/16 , C12Q2563/179 , C12Q2537/143 , C12Q2525/161
摘要： Embodiments of the invention herein described relate to multiplex polynucleotide sequence analysis without the use of size separation methods or blotting. In certain particulars the invention relates to multiplex sequencing using massively parallel sequencing methods, such as pyrosequencing methods and sequencing by synthesis. The invention provides increased throughput and the ability to analyse greater numbers of samples simultaneously or serially on presently available systems, as well as others yet to be developed. In certain of its embodiments the invention relates to the analysis of complex microbial communities, particularly to in-depth analysis thereof in large numbers of samples.
摘要翻译：本文描述的本发明的实施方案涉及多重多核苷酸序列分析，而不使用大小分离方法或印迹。在某些细节中，本发明涉及使用大规模平行测序方法的多重测序，例如焦磷酸测序方法和通过合成测序。本发明提供增加的吞吐量以及在目前可用的系统以及尚待开发的其他系统中同时或连续分析更多数量的样品的能力。在其某些实施方案中，本发明涉及复杂微生物群落的分析，特别是涉及大量样品的深入分析。

86. 发明公开

EP2367959A4 METHODS AND REAGENTS FOR SHORTENING INCUBATION TIMES IN HYBRIDIZATION ASSAYS 有权
标题翻译：方法和试剂在杂交试验异径孵化
公开(公告)号：EP2367959A4
公开(公告)日：2012-10-31
申请号：EP09837958
申请日：2009-12-18
申请人： SIEMENS HEALTHCARE DIAGNOSTICS
发明人： BATTERSBY THOMAS , BAUMEISTER MARK , BROOKS JESSE , KLESHIK FELIX , TAM STACEY
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/682 , C12Q1/6834 , C12Q2565/519 , C12Q2525/161 , C12Q2527/125

87. 发明公开

EP2483425A2 METHODS AND COMPOSITIONS FOR DETECTION OF NUCLEIC ACIDS BASED ON STABILIZED OLIGONUCLEOTIDE PROBE COMPLEXES 有权
标题翻译：基于对限定OLIGONUKLEOTIDSONDENKOMPLEXE方法和组合物对于核酸检测
公开(公告)号：EP2483425A2
公开(公告)日：2012-08-08
申请号：EP10819264.2
申请日：2010-09-15
申请人： Kutyavin, Igor
发明人： Kutyavin, Igor
IPC分类号： C12Q1/68 , C12N15/11 , G06F19/00
CPC分类号： C12Q1/6853 , C12Q2537/162 , C12Q2525/204 , C12Q2525/161
摘要： Provided are nucleic acid detection methods wherein targeted primer extension or products or amplification products incorporate a probe-anchoring modification introduced using a primer incorporating a probe-anchoring primer modification, wherein oligonucleotide detection probes incorporate a probe-anchoring probe modification, the primers and probes designed to place the binding site of the oligonucleotide probe proximate to the probe-anchoring primer modification in the detected target sequence. The probe-anchoring modifications of the probe and the primer-extension product form a stabilized complex comprising a detectible duplex of the probe with the detected target sequence. In certain aspects, the probe-anchoring modified primer also incorporates a probe-directing sequence. The methods allow use of exceptionally short oligonucleotide probes (e.g., 10-mer and shorter) enabling establishment of a complete probe inventory or universal library containing a probe complementary to any target nucleic acid sequence. Real-time and post-amplification detection methods are provided, along with detection kits.

88. 发明公开

EP2467479A2 COMPOSITIONS AND METHODS FOR INTRAMOLECULAR NUCLEIC ACID REARRANGEMENT 有权
标题翻译：组合物和方法分子内重排NUCLEIC
公开(公告)号：EP2467479A2
公开(公告)日：2012-06-27
申请号：EP10768061.3
申请日：2010-08-13
申请人： Population Genetics Technologies LTD.
发明人： BRENNER, Sydney , MIKAWA, Gi , OSBORNE, Robert , SLATTER, Andrew
IPC分类号： C12N15/10
CPC分类号： C12Q1/6874 , C12N15/1065 , C12Q1/6806 , C12Q1/6855 , C12Q1/686 , C12Q2563/179 , C12Q2537/143 , C12Q2535/122 , C12Q2525/161 , C12Q2525/155 , C12Q2525/143
摘要： Aspects of the present invention are drawn to processes for moving a region of interest in a polynucleotide from a first position to a second position with regard to a domain within the polynucleotide, also referred to as a "reflex method". In certain embodiments, the reflex method results in moving a region of interest into functional proximity to specific domain elements present in the polynucleotide (e.g., primer sites and/or MID). Compositions, kits and systems that find use in carrying out the reflex processes described herein are also provided.

89. 发明授权

EP1929045B9 METHODS, COMPOSITIONS AND KITS FOR ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS 失效
标题翻译：用于核酸等温扩增的方法，组合物和试剂盒
公开(公告)号：EP1929045B9
公开(公告)日：2012-06-20
申请号：EP06803026.1
申请日：2006-09-06
申请人： GEN-PROBE INCORPORATED
发明人： BECKER, Michael, M. , LIVEZEY, Kristin, W.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12P19/34 , C12Q1/6853 , C12Q2527/101 , C12Q2537/155 , C12Q2525/161 , C12Q2525/179 , C12Q2537/1376
摘要： Methods and compositions are described for isothermal nucleic acid amplification of a nucleic acid template strand by using an oligonucleotide primer that includes an AT-rich nucleotide sequence and a polymerase having strand displacement activity.
摘要翻译：描述了通过使用包含富含AT的核苷酸序列和具有链置换活性的聚合酶的寡核苷酸引物对核酸模板链的等温核酸扩增的方法和组合物。

90. 发明授权

EP2288726B1 INACTIVATABLE TARGET CAPTURE OLIGOMERS FOR USE IN THE SELECTIVE HYBRIDIZATION AND CAPTURE OF TARGET NUCLEIC ACID SEQUENCES 有权
标题翻译：失活靶标捕获低聚物FOR USE IN靶核酸序列的选择性杂交CATCH
公开(公告)号：EP2288726B1
公开(公告)日：2012-06-13
申请号：EP09747458.9
申请日：2009-05-13
申请人： Gen-Probe Incorporated
发明人： BECKER, Michael, M. , LIVEZEY, Kristin, W.
IPC分类号： C12Q1/68 , G01N33/53
CPC分类号： C12Q1/6816 , C12Q1/6832 , C12Q1/6834 , C12Q1/686 , C12Q2525/161 , C12Q2525/301 , C12Q2565/107 , C12Q2563/179 , C12Q2527/101 , C12Q2537/1373
摘要： An inactivatable target capture oligomer comprising at least: (i) a binding pair member; (ii) a target hybridizing region that is at least 15 nucleobases in length; and (iii) a tag-closing region that is at least 3 nucleobases in length; wherein the tag-closing region nucleic acid sequence is substantially complementary to a portion of the target hybridizing region nucleic acid sequence, and wherein the binding pair member, the target hybridizing region and the tag-closing region are joined as a single molecule.

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