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    • 10. 发明公开
    • QUANTIFICATION OF TARGET NUCLEIC ACID USING COMMON INTERNAL CONTROL
    • 数量ON ON ON EL NU LE LE LE LE LE LE LE LE LE LE LE LE LE
    • EP2992113A1
    • 2016-03-09
    • EP14791659.7
    • 2014-05-02
    • Seegene, Inc.
    • CHUN, Jong YoonLEE, Young Jo
    • C12Q1/68C12N15/11
    • C12Q1/6851C12Q2537/165C12Q2545/101
    • The present invention relates to a method for quantifying a target nucleic acid sequence by use of a common internal control. The present invention allows to determine an absolute initial amount of a target nucleic acid sequence with no use of a standard curve. The present invention amplifies not only a control reaction mixture comprising a known-amount standard and an internal control but also a sample reaction mixture comprising an internal control identical to the internal control in the control reaction mixture and the target nucleic acid sequence to be quantified. In the present invention, a relative ratio of the amount of the standard and the amount of the target nucleic acid sequence is calculated by using the internal control and then an initial amount of the target nucleic acid sequence is determined by using a known amount of the standard.
    • 本发明涉及通过使用共同的内部对照来定量靶核酸序列的方法。 本发明允许不使用标准曲线来确定靶核酸序列的绝对初始量。 本发明不仅扩增了包含已知量标准物和内部对照物的对照反应混合物,而且扩增了包含与对照反应混合物中的内部对照和待定量的靶核酸序列相同的内部对照的样品反应混合物。 在本发明中,使用内部对照计算标准量与目标核酸序列量的相对比例,然后通过使用已知量的目标核酸序列来确定靶核酸序列的初始量 标准。