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71. 发明公开

EP1910397A2 HOT START REVERSE TRANSCRIPTION BY PRIMER DESIGN 审中-公开
标题翻译： DRAFT热启动重发引物
公开(公告)号：EP1910397A2
公开(公告)日：2008-04-16
申请号：EP06800091.8
申请日：2006-07-17
申请人： Applera Corporation
发明人： LAO, Kai Qin , STRAUS, Neil , LIVAK, Kenneth
IPC分类号： C07H21/04
CPC分类号： C12Q1/6876 , C07H21/02 , C07H21/04 , C12N9/00 , C12Q1/6844 , C12Q1/6848 , C12Q1/686 , C12Q2549/101 , C12Q2533/101 , C12Q2525/301
摘要： The present teachings provide methods, compositions, and kits for performing primer extension reactions. In some embodiments, a reverse transcription reaction is performed on a target polynucleotide with a hot start primer comprising a blunt-ended self-complementary stem, and a loop, and extension products form at high temperatures but reduce extension product formation at low temperatures.

72. 发明授权

EP1319719B1 Reversibly modified thermostable enzymes for DNA synthesis and amplification in vitro 有权转让
标题翻译：用于DNA和-Amplifizierung的体外合成可逆性修饰的热稳定酶
公开(公告)号：EP1319719B1
公开(公告)日：2007-04-18
申请号：EP02026712.6
申请日：2002-11-30
申请人： Roche Diagnostics GmbH , F. HOFFMANN-LA ROCHE AG
发明人： Sobek, Harald, Dr. , Greif, Michael
IPC分类号： C12Q1/68 , C12N9/99
CPC分类号： C12Q1/6848 , C12N9/1252 , C12N9/22 , C12N9/99 , C12Q1/686 , C12Q2549/101 , C12Q2521/319 , C12Q2521/101

73. 发明公开

EP1419275A2 MAGNESIUM PRECIPITATE HOT START METHOD FOR MOLECULAR MANIPULATION OF NUCLEIC ACIDS 审中-公开
标题翻译：热启动MAGNESIUMPRÄZIPITATIONSVERFAHREN对于核酸的分子操作
公开(公告)号：EP1419275A2
公开(公告)日：2004-05-19
申请号：EP02763410.4
申请日：2002-08-02
申请人： Barnes, Wayne M.
发明人： Barnes, Wayne M.
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6848 , C12N15/10 , C12N15/1096 , C12P19/34 , C12Q1/686 , C12Q2549/101 , C12Q2527/137 , C12Q2527/125
摘要： The present invention provides methods of performing enzymatic reactions, including PCR, which require the use of magnesium dependent enzymes, including restriction endonucleases, ligases, and reverse transcriptases. The method is based on sequestration of magnesium ions in the form of a precipitate which renders a magnesium dependent enzyme inactive until the appropriate time in the reaction when a certain temperature is reached and the magnesium ions are released from the precipitate. Also provided are kits comprising reagents and instructions for amplifying a target nucleic acid, for DNA digestion and ligation, and for reverse transcription of RNA into cDNA. Furthermore, the kits and reagents of the present invention can be utilized in other reactions requiring magnesium dependent enzymes.

74. 发明公开

EP1319719A1 Reversibly modified thermostable enzymes for DNA synthesis and amplification in vitro 有权转让
标题翻译： Reversibel modifizierte warmabile Enzymefürdie in vitro-Synthese und -Amplifizierung von DNA
公开(公告)号：EP1319719A1
公开(公告)日：2003-06-18
申请号：EP02026712.6
申请日：2002-11-30
申请人： Roche Diagnostics GmbH , F. HOFFMANN-LA ROCHE AG
发明人： Sobek, Harald, Dr. , Greif, Michael
IPC分类号： C12Q1/68 , C12N9/99
CPC分类号： C12Q1/6848 , C12N9/1252 , C12N9/22 , C12N9/99 , C12Q1/686 , C12Q2549/101 , C12Q2521/319 , C12Q2521/101
摘要： The invention relates to a composition comprising a first modified thermostable enzyme exhibiting 3'exonuclease activity but essentially no DNA polymerase activity and a second modified thermostable enzyme exhibiting DNA polymerase activity, whereas the fidelity of an amplification process is enhanced by the use of the composition in an amplification process in comparison to the use of the single second enzyme in an amplification process and, whereas said first and said second modified thermostable enzyme is reversibly modified by an inhibiting agent which results in essentially complete inactivation of enzyme activity, wherein incubation of said first and said second modified thermostable enzyme in an aqueous buffer at alkaline pH at a temperature less than 25 °C for 20 minutes results in no significant increase in the activity of said first and said second modified thermostable enzyme, wherein incubation at a temperature greater than 50 °C in an aqueous buffer at alkaline pH results in at least tow-fold increase in enzyme activity in less than 20 minutes which allow formation of primer extension products.
摘要翻译：本发明涉及一种组合物，其包含显示3'核酸酶活性但基本上不存在DNA聚合酶活性的第一修饰的热稳定酶和显示DNA聚合酶活性的第二修饰的热稳定酶，而扩增过程的保真度通过使用组合物与扩增过程中单一第二酶的使用相比较的扩增过程，而所述第一和第二修饰的热稳定酶由抑制剂可逆地修饰，所述抑制剂导致酶活性基本上完全失活，其中所述第一并且所述第二修饰的热稳定酶在碱性pH在低于25℃的温度下在水性缓冲液中20分钟导致所述第一和所述第二修饰的热稳定酶的活性没有显着增加，其中在大于50℃的温度下孵育 DEG在碱性pH值的水性缓冲液中在少于20分钟内产生至少两倍的酶活性增加，这允许形成引物延伸产物。

75. 发明公开

EP1277841A2 New composition and method for hot start nucleic acid amplification 有权
标题翻译： Neue Zusammensetzung und Methode zur'hot start'Nukleinsäureamplifizierung
公开(公告)号：EP1277841A2
公开(公告)日：2003-01-22
申请号：EP02015234.4
申请日：2002-07-09
申请人： Roche Diagnostics GmbH , F. HOFFMANN-LA ROCHE AG
发明人： Ankenbauer, Waltraud, Dr. , Heindl, Dieter, Dr. , Laue, Frank , Huber, Andreas
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2521/319 , C12Q2525/186 , C12Q2549/101
摘要： The present invention is directed to a new composition for performing a nucleic acid amplification reaction comprising (i) a thermostable DNA-Polymerase, (ii) a thermostable 3'-5' Exonuclease, and (iii) at least one primer for nucleic acid amplification with a modified 3' terminal residue which is not elongated by said thermostable DNA-Polymerase as well as methods for performing a PCR reaction using this composition. Furthermore, the method is directed to kits comprising such a composition.
摘要翻译：本发明涉及一种用于进行核酸扩增反应的新组合物，其包含（i）热稳定的DNA聚合酶，（ii）热稳定的3'-5'核酸外切酶，和（iii）用于核酸扩增的至少一个引物具有未被所述耐热性DNA聚合酶延长的修饰的3'末端残基以及使用该组合物进行PCR反应的方法。此外，该方法涉及包含这种组合物的试剂盒。

76. 发明公开

EP1232502A1 MAGNETIC GLASS PARTICLES, METHOD FOR THEIR PREPARATION AND USES THEREOF 有权
标题翻译：磁性玻璃，方法和用途
公开(公告)号：EP1232502A1
公开(公告)日：2002-08-21
申请号：EP00981267.8
申请日：2000-11-17
申请人： Roche Diagnostics GmbH
发明人： WEINDEL, Kurt , RIEDLING, Michael , GEIGER, Albert
IPC分类号： H01F1/00 , H01F1/44 , C12N15/10 , C12Q1/68 , B03C1/01
CPC分类号： B82Y25/00 , B03C1/01 , C03B19/1065 , C03C1/006 , C03C3/091 , C03C3/093 , C03C12/00 , C03C14/004 , C03C2214/08 , C03C2214/32 , C12N15/1013 , C12Q1/6806 , C12Q1/6816 , C12Q1/6848 , C12Q2561/101 , C23C26/00 , C23C30/00 , H01F1/0054 , H01F1/0063 , H01F1/44 , C12Q2563/155 , C12Q2549/125 , C12Q2549/101
摘要： This invention relates to magnetic nano sized particles having a glass surface. This invention also relates to methods for making them, as well as to suspensions thereof and their uses for the purification of DNA or RNA in particular in automated processes.

77. 发明公开

EP0863213A1 Thermal cycler equipment 失效
标题翻译： Thermische Kreisprozesseinrichtung
公开(公告)号：EP0863213A1
公开(公告)日：1998-09-09
申请号：EP98200769.2
申请日：1992-07-22
申请人： F. HOFFMANN-LA ROCHE AG , THE RESEARCH FOUNDATION OF STATE UNIVERSITY OF NEW YORK
发明人： Bloch, Will , Nuovo, Gerard J.
IPC分类号： C12Q1/68 , C12M1/38 , B01L3/00 , B01L7/00
CPC分类号： C12Q1/6841 , B01L7/52 , C12Q1/686 , C12Q2549/101 , C12Q2543/101 , C12Q2522/101
摘要： Improvements to the in situ polymerase chain reaction (PCR), a process of in vitro enzymatic amplification of specific nudeic acid sequences within the cells where they originate, can be achieved by changing the way that the enzymatic reaction is started. Reaction initiation is delayed until the start of PCR thermal cycling, either by withholding a subset of PCR reagents from the cellular preparation until the preparation has been heated to 50° to 80°C, immediately before thermal cycling is begun, or by adding to the PCR reagents a single-stranded DNA binding protein which blocks reaction at temperatures below about 50°C. If the in situ PCR is performed on cellular preparations already attached to a microscope slide, thermal cyding also is facilitated by use of a thermal cycler sample block or compartment designed optimally to hold the microscope slide and any vapor barrier covering the slide.
摘要翻译：原位聚合酶链反应（PCR）的改进，其可以通过改变酶反应开始的方式来实现在其起源的细胞内体外酶促扩增特定的裸露酸序列的过程。反应开始被延迟直到PCR热循环的开始，无论是通过从细胞制剂中扣留一组PCR试剂，直到制备已被加热至50℃至80℃，立即在热循环开始之前，或通过加入到 PCR试剂是在低于约50℃的温度下阻断反应的单链DNA结合蛋白。如果在已经连接到显微镜载玻片上的细胞制剂上进行原位PCR，则通过使用热循环仪样品块或隔室设计最佳，以保持显微镜载玻片和覆盖幻灯片的任何蒸气屏障。

78. 发明公开

EP0854196A1 Method for the uncoupled, direct, exponential amplification and sequencing of DNA molecules with the addition of a second thermostable DNA polymerase and its application 失效
标题翻译：一种用于非耦合，直接，指数扩增和DNA分子的测序与加入第二热稳定DNA聚合酶和其应用的过程
公开(公告)号：EP0854196A1
公开(公告)日：1998-07-22
申请号：EP97122379.7
申请日：1997-12-18
申请人： BOEHRINGER MANNHEIM GMBH
发明人： Pääbo, Svante Erik Prof. Dr. , Kilger, Christian Alexis Dr.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , Y10S435/81 , C12Q2535/113 , C12Q2535/101 , C12Q2521/101 , C12Q2527/101 , C12Q2549/101 , C12Q2531/113
摘要： Method for sequencing a nucleic acid molecule in a thermocycling reaction which initially comprises a nucleic acid molecule, a first primer, a second primer, a reaction buffer, a first thermostable DNA polymerase, (optionally) a thermostable pyrophosphatase, deoxynucleotides or derivatives thereof and a dideoxynucleotide or a derivative thereof and which is characterized in that the thermocycling reaction additionally contains a second thermostable DNA polymerase which, in comparison to the said first thermostable DNA polymerase, has a reduced ability to incorporate dideoxynucleotides as well as the use of the said method.
摘要翻译：一种用于在热循环反应最初包括核酸分子，第一引物，第二引物，反应缓冲液，第一热塑性表DNA聚合酶测序的核酸分子，（任选地）一个热塑性表焦磷酸酶，脱氧核苷酸或其衍生物的方法和一这是在为特征的双脱氧核苷酸或其衍生物和所有做的热循环反应另外包含第二热塑性表DNA聚合酶其中，相比于所述第一热塑性表的DNA聚合酶，具有掺入双脱氧核苷酸能力下降以及使用所述方法的。

79. 发明公开

EP3157685A1 METHODS FOR GENERATING STABILIZED LYOPHILIZED MATERIALS 无效
标题翻译：产生稳定的冻干材料的方法
公开(公告)号：EP3157685A1
公开(公告)日：2017-04-26
申请号：EP15809313.8
申请日：2015-06-16
申请人： Luminex Corporation
发明人： JOHNSON, Scott , DILLMAN, Jen
IPC分类号： B05D3/10 , C12Q1/25 , C12P19/34 , C12P7/64
CPC分类号： C12Q1/6806 , C12Q1/6848 , C12Q2549/101 , C12Q2563/137 , C12Q2563/149 , C12Q2563/159
摘要： Lyophilized biological reagents, such as enzymes (e.g., PCR reagents) and antibodies, are provided that include a wax component. Thus, in some aspects, a method is provided for storing a biological reagent comprising formulating the reagent into a lyophilized composition including a wax component. Methods for using such lyophilized reagents are likewise provided.
摘要翻译：提供了冻干的生物试剂，例如酶（例如PCR试剂）和抗体，其包含蜡组分。因此，在一些方面中，提供了用于储存生物试剂的方法，其包括将试剂配制成包含蜡组分的冻干组合物。同样提供使用这种冻干试剂的方法。

80. 发明公开

EP2922965A1 METHOD FOR PREVENTING CARRY-OVER CONTAMINATION IN NUCLEIC ACID AMPLIFICATION REACTIONS 审中-公开
标题翻译：维多利亚ZER VERHINDERUNG EINER VERSCHLEPPUNGSKONTAMINATION INNUKLEINSÄURE-AMPLIFIKATIONSREAKINGEN
公开(公告)号：EP2922965A1
公开(公告)日：2015-09-30
申请号：EP13780270.8
申请日：2013-10-08
申请人： Courtagen Life Sciences Inc.
发明人： MCKERNAN, Kevin, J.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6848 , C12Q1/686 , C12Q2521/331 , C12Q2525/117 , C12Q2525/131 , C12Q2549/101
摘要： The invention is directed to methods of removing amplicons of non target and/or target nucleic acid sequences having one or more modified (
e.g ., methylated) nucleotides from a sample wherein the sample comprises the non target nucleic acid and a target nucleic acid sequence to be amplified.
摘要翻译：本发明涉及从样品中除去具有一个或多个经修饰（例如，甲基化）核苷酸的非靶和/或靶核酸序列的扩增子的方法，其中样品包含非靶核酸和靶核酸序列为放大。

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