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1. 发明授权

EP3052653B1 A METHOD OF IDENTIFYING OR PRODUCING AN APTAMER 有权
公开(公告)号：EP3052653B1
公开(公告)日：2018-12-05
申请号：EP14792753.7
申请日：2014-10-02
申请人： Tolle, Fabian , Friedrich, Felix , Heckel, Alexander , Mayer, Günter
发明人： Tolle, Fabian , Friedrich, Felix , Heckel, Alexander , Mayer, Günter
IPC分类号： C12Q1/68 , C12N15/10 , C12N15/115
CPC分类号： C12Q1/6811 , C12N15/115 , C12N2310/16 , C12N2320/13 , C12Q2525/117 , C12Q2525/205 , C12Q2541/101
摘要： The invention relates to amethod of identifying or producing an aptamer,and a reagent comprising a nucleic acid ligand capable of binding a target sample, wherein the nucleic acid ligand comprises at least one nucleobase modified to contain an azide-alkyne chemical group.

2. 发明公开

EP3330277A3 CYTIDINE-5-CARBOXAMIDE MODIFIED NUCLEOTIDE COMPOSITIONS AND METHODS RELATED THERETO 审中-公开
公开(公告)号：EP3330277A3
公开(公告)日：2018-09-26
申请号：EP18153515.4
申请日：2014-11-19
申请人： Somalogic, Inc.
发明人： ROHLOFF, John , JANJIC, Nebojsa , GAWANDE, BharatNathu
IPC分类号： C07H19/06 , C07H19/073 , C07H19/10 , C07H21/00 , C07H1/00 , C12Q1/6811
CPC分类号： C07H19/06 , C07H1/00 , C07H19/073 , C07H19/10 , C07H21/00 , C12Q1/6811 , C12Q2525/117
摘要： Described herein are 5-position modified cytosines of formula I, nucleotides and nucleosides as well as phosphoramidites and triphosphates derivatives thereof. Further provided are methods of making and using the same, and compositions and uses of the modified nucleosides as part of a nucleic acid molecule (e.g., aptamer). Those modified nucleotides, bearing an N-substituted-carboxamine group at the 5 -position, are valuable tools for improving in vitro selection of protein-binding aptamers (SELEX process).

wherein
R is independently a -(CH 2 ) n , wherein n is 0,1,2,3,4,5,6,7,8,9 or 10;
R X1 is independently selected from the group consisting of

3. 发明授权

EP2873731B1 NUCLEIC ACID PROBE, METHOD FOR DESIGNING NUCLEIC ACID PROBE, AND METHOD FOR DETECTING TARGET SEQUENCE 有权
公开(公告)号：EP2873731B1
公开(公告)日：2018-08-29
申请号：EP13820349.2
申请日：2013-07-12
申请人： Kabushiki Kaisha Dnaform
发明人： HAYASHIZAKI, Yoshihide , HANAMI, Takeshi , SOMA, Takahiro , KIMURA, Yasumasa , KANAMORI, Hajime , MITANI, Yasumasa
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C09B23/04 , C09B23/06 , C12N2330/30 , C12Q1/6827 , C12Q2525/117 , C12Q2535/131 , C12Q2537/101 , C12Q2563/107 , C12Q2565/1015 , C12Q2565/107
摘要： The present invention provides a nucleic acid probe that can achieve high detection sensitivity and high specificity in mutation detection, mismatch detection, etc. by the PCR method, a method for designing such a nucleic acid probe, and a method for detecting a target sequence. The nucleic acid probe includes a nucleic acid molecule, and the nucleic acid molecule includes a plurality of fluorescent dye moieties that exhibit an excitonic effect. At least two of the fluorescent dye moieties that exhibit an excitonic effect are bound to the same base or two adjacent bases in the nucleic acid molecule with each fluorescent dye moiety being bound via a linker (a linking atom or a linking atomic group). The extension-side end of the nucleic acid molecule is chemically modified, thereby preventing an extension reaction of the nucleic acid molecule.

4. 发明公开

EP3350347A1 METHODS AND MATERIALS FOR DETECTION OF MUTATIONS 有权
公开(公告)号：EP3350347A1
公开(公告)日：2018-07-25
申请号：EP16777896.8
申请日：2016-09-14
申请人： Pentabase APS
发明人： CHRISTENSEN, Ulf Bech
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q2525/101 , C12Q2525/113 , C12Q2525/117 , C12Q2525/121 , C12Q2525/203 , C12Q2535/125 , C12Q2537/163
摘要： Provided herein are specially modified blocking nucleotides allowing for the sensitive detection of low copies of variant sequences, while significantly reducing signals from non-variant sequences that are similar but not identical to the variant sequence. These nucleotides can be used to detect rare variants in a sample mixture, as described in the present methods.

5. 发明授权

EP2307565B1 REVERSIBLE NUCLEOSIDES AND NUCLEOTIDES TERMINATORS AND THEIR USE IN DNA SEQUENCING 有权转让
标题翻译：可逆核苷酸和核苷酸终止子及其在DNA测序中的应用
公开(公告)号：EP2307565B1
公开(公告)日：2017-11-29
申请号：EP09763641.9
申请日：2009-06-11
申请人： Lasergen, Inc.
发明人： LITOSH, Vladislav, A. , HERSH, Megan, N. , STUPI, Brian, P. , WU, Weidong , METZKER, Michael, L.
IPC分类号： C12Q1/68 , C07H19/14 , C07H19/20
CPC分类号： C12Q1/6869 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C12Q2525/117 , C12Q2563/107

6. 发明公开

EP3234180A1 ALLELE-SPECIFIC AMPLIFICATION OF NUCLEIC ACIDS USING BLOCKING OLIGONUCLEOTIDES FOR WILD TYPE SUPPRESSION 审中-公开
标题翻译：使用阻断寡核苷酸进行野生型抑制的核酸特异性扩增
公开(公告)号：EP3234180A1
公开(公告)日：2017-10-25
申请号：EP15808657.9
申请日：2015-12-15
申请人： Roche Diagnostics GmbH , F. Hoffmann-La Roche AG
发明人： WILL, Stephen G.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q1/6806 , C12Q2525/117 , C12Q2527/107 , C12Q2537/159 , C12Q2537/163
摘要： A method is provided for allele-specific amplification, utilizing a blocking oligonucleotide including at least one nucleotide with a base covalently modified at the exocyclic amino group, the blocking oligonucleotide being perfectly complementary to a wild type (WT) sequence when hybridized forming a first complex having a first melting temperature (Tm), the blocking oligonucleotide being partially non-complementary, at one or more nucleotides, to a target mutant (MT) sequence when hybridized forming a second complex having a second melting temperature (Tm), wherein the first Tm is higher than the second Tm and having at least one nucleotide with a base covalently modified at the exocyclic amino group, wherein the blocking oligonucleotide becomes unhybridized from the target MT sequence during amplification but remains hybridized with the WT sequence inhibiting amplification of the WT sequence utilizing a polymerase lacking 5′-3′ nuclease activity.
摘要翻译：提供了用于等位基因特异性扩增的方法，所述方法利用包含至少一个核苷酸的封闭寡核苷酸以及在环外氨基处共价修饰的碱基，当杂交形成第一复合物时，封闭寡核苷酸与野生型（WT）序列完全互补具有第一解链温度（Tm），当形成具有第二解链温度（Tm）的第二复合物时，所述阻断寡核苷酸在一个或多个核苷酸处与靶突变体（MT）序列部分非互补，其中所述第一解链温度 Tm高于第二Tm且具有至少一个核苷酸，所述核苷酸在环外氨基处共价修饰的碱基，其中所述阻断寡核苷酸在扩增过程中与靶MT序列未杂交，但仍与抑制WT序列扩增的WT序列杂交利用缺乏5'-3'核酸酶活性的聚合酶。

7. 发明公开

EP3227462A1 METHOD FOR IN SITU DETERMINATION OF NUCLEIC PROXIMITY 有权转让
标题翻译：原位测定核接近度的方法
公开(公告)号：EP3227462A1
公开(公告)日：2017-10-11
申请号：EP15865948.2
申请日：2015-12-01
申请人： The Broad Institute Inc. , Baylor College of Medicine
发明人： LIEBERMAN-AIDEN, Erez , RAO, Suhas , STAMENOVA, Elena , DUDCHENKO, Olga , LANDER, Eric
IPC分类号： C12Q1/68 , C40B20/00 , C40B30/04
CPC分类号： C12Q1/6869 , C12Q1/6806 , C12Q2521/501 , C12Q2525/117 , C12Q2535/122 , C12Q2563/131 , C40B20/00 , C40B30/04
摘要： Disclosed is an in situ method for detecting spatial proximity relationships between nucleic acid sequences, such as DNA, in a cell. The method includes: providing a sample of one or more cells comprising nucleic acids; fragmenting the nucleic acids present in the cells that leaves 5′ overhanging ends; filling in the overhanging ends with at least one labeled nucleotide; joining the filled in end of the fragmented nucleic acids that are in close physical proximity to create one or more end joined nucleic acid fragments having a junction; isolating the one or more end joined nucleic acid fragments using the labeled nucleotide; and determining the sequence at the junction of the one or more end joined nucleic acid fragments.
摘要翻译：公开了用于检测细胞中的核酸序列例如DNA之间的空间邻近关系的原位方法。该方法包括：提供一个或多个包含核酸的细胞的样品; 将存在于存在于5'突出末端的细胞中的核酸片段化; 用至少一个标记的核苷酸填充突出端; 连接紧密物理接近的片段化核酸的填充末端以产生具有连接处的一个或多个末端连接的核酸片段; 使用标记的核苷酸分离一个或多个末端连接的核酸片段; 并确定一个或多个末端连接的核酸片段连接处的序列。

8. 发明公开

EP3071588A4 CYTIDINE-5-CARBOXAMIDE MODIFIED NUCLEOTIDE COMPOSITIONS AND METHODS RELATED THERETO 审中-公开
标题翻译： CYTIDIN-5-CARBOXAMID-MODIFIZIERTE NUKLEOTIDZUSAMMETETZUNGEN UNDZUGEHÖRIGEVERFAHREN
公开(公告)号：EP3071588A4
公开(公告)日：2017-08-02
申请号：EP14864118
申请日：2014-11-19
申请人： SOMALOGIC INC
发明人： ROHLOFF JOHN , JANJIC NEBOJSA , GAWANDE BHARATNATHU
IPC分类号： C07H19/06 , C07H1/00 , C07H21/00 , C12Q1/68
CPC分类号： C07H19/06 , C07H1/00 , C07H21/00 , C12Q1/6811 , C12Q2525/117
摘要： Described herein are 5-position modified cytosines of formula I, nucleotides and nucleosides as well as phosphoramidites and triphosphates derivatives thereof. Further provided are methods of making and using the same, and compositions and uses of the modified nucleosides as part of a nucleic acid molecule (e.g., aptamer). Those modified nucleotides, bearing an N-substituted-carboxamine group at the 5 -position, are valuable tools for improving in vitro selection of protein-binding aptamers (SELEX process). wherein R is independently a -(CH 2 ) n , wherein n is 0,1,2,3,4,5,6,7,8,9 or 10; R X1 is independently selected from the group consisting of
摘要翻译：本文描述了5位修饰的胞嘧啶核苷酸和核苷以及其亚磷酰胺和三磷酸酯衍生物。进一步提供了制备和使用它们的方法，以及修饰的核苷作为核酸分子（例如适体）的一部分的组合物和用途。

9. 发明公开

EP3187598A1 METHOD FOR MODIFYING NUCLEIC ACIDS 有权
标题翻译：修饰核酸的方法
公开(公告)号：EP3187598A1
公开(公告)日：2017-07-05
申请号：EP17155621.0
申请日：2012-01-20
申请人： Takara Bio Inc.
发明人： Yamamoto, Junko , Kubo, Keiko , Uemori, Takashi , Mukai, Hiroyuki , Asada, Kiyozo
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： C12N15/01 , C12Q1/6848 , C12Q1/686 , C12Q1/689 , C12Q2523/101 , C12Q2525/117
摘要： The present invention pertains to: a method for modifying a nucleic acid contained in a sample, said method including a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, said method including the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.
摘要翻译：本发明涉及修饰样品中所含核酸的方法，所述方法包括在酸性多糖和/或核苷酸存在下使样品与核酸修饰剂接触的步骤; 所述方法包括以下步骤：（a）根据用于修饰包含在所述样品中的核酸的方法对样品中包含的核酸进行修饰的步骤; 样品，其包括在酸性多糖和/或核苷酸存在下使样品与核酸修饰剂接触的步骤; 和（b）在步骤（a）之后选择性检测来自样品的未修饰核酸的步骤。本发明还涉及用于这些方法的试剂盒和组合物。

10. 发明公开

EP3174996A1 IMPROVED NUCLEIC ACID SAMPLE ANALYSIS USING CONVERTIBLE TAGS 审中-公开
标题翻译：用可转换标记改进核酸样品分析
公开(公告)号：EP3174996A1
公开(公告)日：2017-06-07
申请号：EP15744329.2
申请日：2015-07-28
申请人： Cambridge Epigenetix Limited
发明人： OST, Tobias William Barr , HAMILTON, Russell Smith
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6869 , C12Q1/6876 , C12Q2600/154 , C12Q2523/125 , C12Q2525/117 , C12Q2525/191 , C12Q2563/185
摘要： The inventors have developed a set of molecular tag adaptors allowing the history of a sample to be followed. The adaptors have a region having more than one type of cytosine base.
摘要翻译：本发明人开发了一组允许遵循样品历史的分子标签适配器。衔接子具有一个以上一种胞嘧啶碱基的区域。

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