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1. 发明公开

EP3388530A1 NUCLEIC ACID MODIFICATION AND IDENTIFICATION METHOD 审中-公开
公开(公告)号：EP3388530A1
公开(公告)日：2018-10-17
申请号：EP17166629.0
申请日：2017-04-13
申请人： IMBA-Institut für Molekulare Biotechnologie GmbH
发明人： AMERES, Stefan L. , REICHHOLF, Brian , HERZOG, Veronika A.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2525/101 , C12Q2525/117
摘要： The invention provides a method of identifying a polynucleic acid (PNA) comprising the steps of providing a PNA; modifying one or more nucleobases of the PNA by addition or removal of a hydrogen bonding partner, thereby altering the base pairing capacity of the one or more nucleobases; base pairing a complementary nucleic acid to the PNA, including base pairing to at least one modified nucleobase; identifying the sequence of the complementary nucleic acid at least at the position that is complementary to at least one modified nucleobase.

2. 发明授权

EP3036343B1 HELICASE DEPENDENT AMPLIFICATION OF DNA MOLECULES USING NUCLEOTIDE ANALOGS 失效
公开(公告)号：EP3036343B1
公开(公告)日：2018-09-19
申请号：EP14837579.3
申请日：2014-08-14
申请人： Benner, Steven A. , Yang, Zunyi
发明人： Benner, Steven A. , Yang, Zunyi
IPC分类号： C12Q1/6853 , C12Q1/6844 , C12P19/34
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q1/6853 , C12Q2521/513 , C12Q2525/101 , C12Q2525/117 , C12Q2521/101
摘要： This invention covers processes for the isothermal amplification of DNA molecules having a preselected sequence. It is based on the unexpected discovery that primers having, at some positions, adenine substituted by 2-aminopurine or diaminopurine, guanine by inosine, thymine by 2-thiothymine, and cytosine by N4-ethylcytosine (“SAMRS nucleotides”) were accepted by enzymes used in the standard helicase-dependent amplification (HDA). Further unexpected was the discovery that target nucleotides are efficiently amplified in an HDA-like process (hereinafter abbreviated as simply HDA) using substituted primers. Also discovered was the diminution of spurious products through the use of SAMRS-substituted primers.

3. 发明公开

EP3330277A2 CYTIDINE-5-CARBOXAMIDE MODIFIED NUCLEOTIDE COMPOSITIONS AND METHODS RELATED THERETO 审中-公开
公开(公告)号：EP3330277A2
公开(公告)日：2018-06-06
申请号：EP18153515.4
申请日：2014-11-19
申请人： Somalogic, Inc.
发明人： ROHLOFF, John , JANJIC, Nebojsa , GAWANDE, BharatNathu
IPC分类号： C07H19/06 , C07H19/073 , C07H19/10 , C07H21/00 , C07H1/00 , C12Q1/6811
CPC分类号： C07H19/06 , C07H1/00 , C07H19/073 , C07H19/10 , C07H21/00 , C12Q1/6811 , C12Q2525/117
摘要： Described herein are 5-position modified cytosines of formula I, nucleotides and nucleosides as well as phosphoramidites and triphosphates derivatives thereof. Further provided are methods of making and using the same, and compositions and uses of the modified nucleosides as part of a nucleic acid molecule (e.g., aptamer). Those modified nucleotides, bearing an N-substituted-carboxamine group at the 5 -position, are valuable tools for improving in vitro selection of protein-binding aptamers (SELEX process).

wherein
R is independently a -(CH 2 ) n , wherein n is 0,1,2,3,4,5,6,7,8,9 or 10;
R X1 is independently selected from the group consisting of

4. 发明公开

EP3284832A3 METHOD FOR GENERATING APTAMERS WITH IMPROVED OFF-RATES 有权转让
公开(公告)号：EP3284832A3
公开(公告)日：2018-03-07
申请号：EP17181688.7
申请日：2008-07-17
申请人： Somalogic, Inc.
发明人： ZICHI, Dominic , WILCOX, Sheri K. , BOCK, Chris , SCHNEIDER, Daniel J. , EATON, Bruce , GOLD, Larry
IPC分类号： C12Q1/68 , C12N15/115 , C12Q1/6811 , C12N15/10 , C12N15/11
CPC分类号： C12Q1/6811 , C12N15/1048 , C12N15/111 , C12N15/115 , C12N2310/16 , C12N2320/13 , C12Q2541/101 , C12Q2525/205 , C12Q2525/117
摘要： The present disclosure describes improved SELEX methods for producing aptamers that are capable of binding to target molecules and improved photoSELEX methods for producing photoreactive aptamers that are capable of both binding and covalently cross linking to target molecules. Specifically, the present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. In addition, the disclosure describes aptamer constructs that include a variety of functionalities, including a cleavable element, a detection element, and a capture or immobilization element.

5. 发明公开

EP3286339A2 OLIGONUCLEOTIDE-BASED PROBES FOR DETECTION OF CIRCULATING TUMOR CELL NUCLEASES 审中-公开
公开(公告)号：EP3286339A2
公开(公告)日：2018-02-28
申请号：EP16784083
申请日：2016-04-25
申请人： UNIV OF IOWA RESEARCH FOUNDATION , THE BOARD OF TRUSTEES OF THE UNIV OF ILLINOIS A BODY CORPORATE AND POLITIC OF THE STATE OF ILLINOIS
发明人： GIANGRANDE PALOMA H , MCNAMARA JAMES O , DICKEY DAVID D , OZER HOWARD , KRUSPE SVEN
IPC分类号： C12Q1/68 , G01N33/50
CPC分类号： C12Q1/68 , C12Q1/6816 , C12Q1/6886 , C12Q2525/117 , C12Q2525/125
摘要： The present invention relates to a rapid detection of circulating tumor cell (CTC)-associated nuclease activity with chemically modified nuclease substrate probes and compositions useful in detection assays.

6. 发明公开

EP3283638A1 METHODS FOR DETECTING AGGLUTINATION AND COMPOSITIONS FOR USE IN PRACTICING THE SAME 有权
标题翻译：检测凝血酶的方法和用于治疗凝血酶的组合物
公开(公告)号：EP3283638A1
公开(公告)日：2018-02-21
申请号：EP16780915.1
申请日：2016-04-15
申请人： The Regents of the University of California
发明人： ROBINSON, Peter , TSAI, Cheng-Ting , BERTOZZI, Carolyn
IPC分类号： C12P19/34 , C12Q1/68 , G01N33/53
CPC分类号： C12Q1/6804 , C12Q1/6806 , C12Q1/686 , C12Q2525/117 , C12Q2525/155 , C12Q2525/197 , C12Q2531/113 , G01N33/54306
摘要： Methods are provided for detecting antigen binding agents in samples. Aspects of the methods include detection of the aggregation of antigen binding agents with polynucleotide-bound antigens by sensitive proximity-based association of the antigen-bound polynucleotides. Aspects of the methods also include methods for the detection of such proximity-based association through nucleic acid amplification. In addition, compositions, e.g., reagents, kits, and devices, useful in practicing various embodiments of the methods are provided.

7. 发明公开

EP3283630A1 OLIGONUCLEOTIDE FRAGMENT, AND METHOD AS WELL AS APPLICATION OF SELECTIVE AMPLIFICTION OF VARIANT OF TARGET NUCLEIC ACID SEQUENCE USING THE SAME 有权
标题翻译：寡核苷酸片段和方法以及使用相同核酸序列变体的选择性扩增的应用
公开(公告)号：EP3283630A1
公开(公告)日：2018-02-21
申请号：EP15888914.7
申请日：2015-06-18
申请人： Nuhigh Biotechnologies Co., Ltd
发明人： BI, Wanli , WANG, Zhifeng , XU, Xin
IPC分类号： C12N15/11 , C12N15/10 , C12Q1/68
CPC分类号： C12Q1/6858 , C12Q1/6853 , C12Q2525/186 , C12Q2525/301 , C12Q2525/107 , C12Q2525/125 , C12Q2525/113 , C12Q2525/117
摘要： Provided is a method for selective amplification of a variant of a target nucleic acid sequence. Also provided is an oligonucleotide fragment with a particular structure which is used in the the method, the oligonucleotide fragment includes a primer region and a variant recognition region.

8. 发明公开

EP3036336A4 METHODS AND COMPOSITIONS FOR CDNA SYNTHESIS AND SINGLE-CELL TRANSCRIPTOME PROFILING USING TEMPLATE SWITCHING REACTION 有权转让
标题翻译： VERFAHREN UND ZUSAMMENSETZUNGENFÜRCDNA-SYNTHESE UND EINZELZELLEN-TRANSKRIPTOMPROFILIERUNG MITHILFE EINER SCHABLONENSCHALTREAKTION
公开(公告)号：EP3036336A4
公开(公告)日：2017-06-28
申请号：EP14837396
申请日：2014-08-22
申请人： LUDWIG INST FOR CANCER RES LTD
发明人： SANDBERG RICKARD , PICELLI SIMONE , FARIDANI OMID R
IPC分类号： C12P19/34 , C07H21/04
CPC分类号： C12Q1/6883 , C12P19/34 , C12Q1/6809 , C12Q1/6853 , C12Q2600/158 , C12Q2521/107 , C12Q2525/117
摘要： This application discloses methods for cDN′A synthesis with improved reverse transcription, template switching and preamplification to increase both yield and average length of cDNA libraries generated from individual cells. The new methods include exchanging a single nucleoside residue for a locked nucleic acid (INA) at the TSO 3′ end, using a methyl group donor, and/or a MgCb concentration higher than conventionally used. Single-cell transcriptome analyses incorporating these differences have full-length coverage, improved sensitivity and accuracy, have less bias and are more amendable to cost-effective automation. The invention also provides cDNA molecules comprising a locked nucleic acid at the 3′-end, compositions and cDNA libraries comprising these cDNA molecules, and methods for single-cell transcriptome profiling.
摘要翻译：该申请公开了用于cDN'A合成的方法，其具有改进的逆转录，模板转换和预扩增以增加从单个细胞产生的cDNA文库的产量和平均长度。新方法包括使用甲基供体和/或高于常规使用的MgCl 2浓度，在TSO 3'末端交换单核苷酸残基以获得锁核酸（INA）。包含这些差异的单细胞转录组分析具有全长覆盖范围，提高了灵敏度和准确性，偏差较小，并且对经济高效的自动化更加可修改。本发明还提供了在3'端包含锁定核酸的cDNA分子，包含这些cDNA分子的组合物和cDNA文库，以及用于单细胞转录组特征分析的方法。

9. 发明公开

EP3180445A1 MULTIPLEX TRANSCRIPTOME ANALYSIS 审中-公开
标题翻译：多重复制文本分析
公开(公告)号：EP3180445A1
公开(公告)日：2017-06-21
申请号：EP15753859.6
申请日：2015-08-14
申请人： Life Technologies Corporation
发明人： BRAMLETT, Kelli , LEAMON, John , ANDERSEN, Mark , THORNTON, Michael
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6846 , C12Q1/6855 , C12Q2525/117 , C12Q2535/10 , C12Q2537/143 , C12Q2525/191 , C12Q2563/185
摘要： In some embodiments, the disclosure relates generally to methods, compositions, systems, apparatuses and kits comprising a multiplex nucleic acid amplification reaction that employs a plurality (e.g., hundreds, thousands, tens-of-thousands or hundreds-of-thousands) of different target-specific primer pairs that enable substantially simultaneous amplification of a plurality of different target sequences-of-interest in a single reaction mixture. In some embodiments, the multiplex nucleic acid amplification reaction generates a plurality of amplicons having sequences derived from a sample containing RNA or DNA, including whole transcriptome or genomic samples. In some embodiments, the sequences and abundances of at least some of the plurality of amplicons are characterized, optionally simultaneously or through a single assay, by suitable detection methods, including sequencing or other procedures known in the art.
摘要翻译：在一些实施方案中，本公开内容一般涉及包含多重核酸扩增反应的方法，组合物，系统，装置和试剂盒，所述多重核酸扩增反应使用多个（例如数百，数千，数万或数百）目标特异性引物对，其能够基本上同时扩增单个反应混合物中的多个不同的目标靶序列。在一些实施方案中，多重核酸扩增反应产生多个扩增子，所述扩增子具有来自含有RNA或DNA的样品的序列，包括全转录组或基因组样品。在一些实施方案中，通过合适的检测方法，包括测序或本领域已知的其他程序，任选地同时或通过单个测定来表征多个扩增子中的至少一些的序列和丰度。

10. 发明公开

EP3036343A4 HELICASE DEPENDENT AMPLIFICATION OF DNA MOLECULES USING NUCLEOTIDE ANALOGS 失效
标题翻译： HELICASE-ABHÄNGIGEAMPLIFIKATION VON DNAMOLEKÜLENMITHILFE VON NUKLEOTIDANALOGA
公开(公告)号：EP3036343A4
公开(公告)日：2017-05-31
申请号：EP14837579
申请日：2014-08-14
申请人： BENNER STEVEN A , YANG ZUNYI
发明人： BENNER STEVEN A , YANG ZUNYI
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q1/6853 , C12Q2521/513 , C12Q2525/101 , C12Q2525/117 , C12Q2521/101
摘要： This invention covers processes for the isothermal amplification of DNA molecules having a preselected sequence. It is based on the unexpected discovery that primers having, at some positions, adenine substituted by 2-aminopurine or diaminopurine, guanine by inosine, thymine by 2-thiothymine, and cytosine by N4-ethylcytosine (“SAMRS nucleotides”) were accepted by enzymes used in the standard helicase-dependent amplification (HDA). Further unexpected was the discovery that target nucleotides are efficiently amplified in an HDA-like process (hereinafter abbreviated as simply HDA) using substituted primers. Also discovered was the diminution of spurious products through the use of SAMRS-substituted primers.
摘要翻译：本发明涵盖了具有预选序列的DNA分子的等温扩增过程。它基于意想不到的发现，即在某些位置具有由2-氨基嘌呤或二氨基嘌呤取代的腺嘌呤，由肌苷取代鸟嘌呤，由2-硫代胸腺嘧啶取代胸腺嘧啶和由N4-乙基胞嘧啶取代胞嘧啶（“SAMRS核苷酸”）的引物被酶接受用于标准解旋酶依赖性扩增（HDA）。更令人意想不到的是，发现目标核苷酸在使用取代引物的HDA样方法（以下简称为HDA）中被有效扩增。还发现通过使用SAMRS取代的引物减少了假产物。

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