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    • 65. 发明公开
    • SERS NANOTAG ASSAYS
    • SERS-NANOETIKETT-AUSRICHTUNGEN
    • EP2147295A4
    • 2012-04-04
    • EP08826290
    • 2008-04-18
    • BECTON DICKINSON CO
    • NATAN MICHAEL J
    • G01N21/65C40B70/00
    • C40B70/00B01J2219/00572B01J2219/00576B01J2219/00722B01J2219/00725B82Y20/00B82Y30/00G01N21/658G01N2201/06193Y10S977/773
    • A method of producing a surface enhanced Raman scattering spectrum which is useful for certain types of assays, in particular proximity assays. The method includes providing two SERS-active nanoparticles. The first SERS-active nanoparticle will absorb a photon at a first wavelength and emit a Raman-shifted photon at a second wavelength. The second SERS-active nanoparticle will absorb a photon at the second wavelength and emit a Raman-shifted photon at a third wavelength. Accordingly, when the first and second SERS-active nanoparticles are proximate to one another and the first SERS-active nanoparticle is illuminated at the first wavelength a Raman-shifted photon at the second wavelength may be emitted. This photon can be absorbed by the second SERS-active nanoparticle causing detectable emission of a second Raman-shifted photon at the third wavelength. Various assays may be designed based upon the above. Proximity assays using two SERS-active nanoparticles will have advantageous background signal characteristics.
    • 一种产生表面增强拉曼散射光谱的方法,其可用于某些类型的测定,特别是邻近测定。 该方法包括提供两种SERS活性纳米粒子。 第一SERS活性纳米颗粒将吸收第一波长的光子并且在第二波长处发射拉曼移动的光子。 第二SERS活性纳米粒子将吸收第二波长的光子,并在第三波长处发射拉曼光子。 因此,当第一和第二SERS活性纳米颗粒彼此靠近并且第一SERS活性纳米颗粒在第一波长被照射时,可以发射第二波长的拉曼移动的光子。 该光子可以被第二SERS活性纳米粒子吸收,导致在第三波长处的第二拉曼光子的可检测发射。 可以基于上述设计各种测定。 使用两个SERS活性纳米粒子的接近测定将具有有利的背景信号特征。