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61. 发明公开

EP1623025A1 GLUKOSE DEHYDROGENASE UND IHRE HERSTELLUNG 有权
标题翻译：葡萄糖脱氢酶及其生产
公开(公告)号：EP1623025A1
公开(公告)日：2006-02-08
申请号：EP04729624.9
申请日：2004-04-27
申请人： Bayer Technology Services GmbH
发明人： MEISSNER, Ruth , WEICHEL, Walter , KOCH, Rainhard , BACHMATOVA, Irina , MARCINKEVICIENE, Liucija , MESKIENE, Rita , SEMENAITE, Rasa , CASAITE, Vida , MESKYS, Rolandas
IPC分类号： C12N9/04 , C12Q1/32 , C12Q1/54 , C12N15/53 , C12N15/63
CPC分类号： C12N9/0006 , C12Q1/006 , C12Q1/32 , Y10T436/144444
摘要： The invention relates to novel PQQ-dependent soluble glucose dehydrogenases (sPQQGDH) from Acinetobacter and a method for the production thereof by means of overexpression in adequate microbial expression systems.

62. 发明公开

EP1551989A1 RAPID COLIFORM DETECTION SYSTEM 审中-公开
标题翻译：快速检测系统的大肠菌群
公开(公告)号：EP1551989A1
公开(公告)日：2005-07-13
申请号：EP02808004.2
申请日：2002-10-17
申请人： Conestoga-Rovers & Associated Limited
发明人： VAN DYKE, Michele, I. , PALMATEER, Garry, A. , PINTAR, Katarina, D., M.
IPC分类号： C12Q1/00 , C12Q1/04 , C12Q1/54 , C12Q1/22 , C12M1/12
CPC分类号： C12Q1/54 , C12Q1/04 , C12Q1/10
摘要： A system for rapidly determining the presence or quantity of coliform bacteria in a water sample using the enzymes β-D-galactosidase and β-D-glucuronidase. The system includes a first filter means for separating bacteria from the sample and a broth for culturing the bacteria including an inducing agent for inducing enzyme production. A second filter means is used to separate the cultured bacteria from the broth. A lysing agent is exposed to the bacteria on the second filter and incubated with a chemiluminogenic substrate of the enzyme to produce a chemiluminescent product. Light emission is initiated from the second filter means and the emitted light is detected or measured directly from the second filter means using a luminometer adapted to receive the second filter means. The system is especially effective at improving the sensitivity and specificity of the assay by increasing the signal received from encapsulated target organisms and reducing the interference from non-target organisms that may be present in the sample.

63. 发明公开

EP1287160A4 MEASUREMENTS OF ENZYMATIC ACTIVITY IN A SINGLE, INDIVIDUAL CELL IN POPULATION 审中-公开
标题翻译：在每个单个细胞细胞群酶活性的测量
公开(公告)号：EP1287160A4
公开(公告)日：2004-12-29
申请号：EP01934272
申请日：2001-05-17
申请人： UNIV BAR ILAN
发明人： SUNRAY MERAV , ZURGIL NAOMI , DEUTSCH MORDECHAI
IPC分类号： G01N21/77 , C12Q1/00 , C12Q1/02 , C12Q1/26 , C12Q1/28 , C12Q1/37 , C12Q1/44 , C12Q1/527 , G01N21/78 , C12Q1/54 , G01N33/50
CPC分类号： C12Q1/00
摘要： A process for measuring enzymatic activity in an identified, isolated, intact, single, viable cell. Each of the viable cells is placed within individual identified locations on a carrier of a cytometer having means to measure enzymatic activity of a single viable cell placed in an identified location. The identified isolated cell is exposed to a substrate of an enzyme to be measured, and the rate of product formed or released following every exposure of the cell to same or different concentrations of the substrate is measured. The isolated cell may be exposed to a sequence of at least two different concentrations of the substrate, and for each exposure the rate of product formed or released, is measured

64. 发明公开

EP1470133A1 ELECTROCHEMICAL SACCHARIDE SENSOR 有权
标题翻译：电化学糖
公开(公告)号：EP1470133A1
公开(公告)日：2004-10-27
申请号：EP03707635.3
申请日：2003-01-29
申请人： Beckman Coulter, Inc.
发明人： JAMES, Tony, D. , ARIMORI, Susumu
IPC分类号： C07F5/02 , C12Q1/54
CPC分类号： C12Q1/54 , C07F17/02
摘要： The invention provides compounds 1/4/6 for detecting sacchharides by electrochemistry. The compounds have a structure that includes a first boronic acid group attached by a first linker group (B) to a first tertiary amine. The first tertiary amine is attached to a reporter group that includes an organometalic reporter moiety. A spacer group (R2) attaches the first tertiary amine to a second tertiary amine. The second tertiary amine is attached to an R group and a second linker group (B). The second linker group (B) is attached to a second boronic acid group. In preferred embodiments, the compound 1/4/6 selectively detects glucose and has a higher relative stability constant for glucose than for other saccharides.

65. 发明公开

EP1466007A1 NON-OR MINIMALLY INVASIVE MONITORING METHODS 审中-公开
标题翻译：无创或微创监测方法
公开(公告)号：EP1466007A1
公开(公告)日：2004-10-13
申请号：EP02786772.0
申请日：2002-12-13
申请人： Powderject Research Limited
发明人： BURKOTH, Terry, L.
IPC分类号： C12Q1/00 , C12Q1/54
CPC分类号： G01N33/54313 , A61B5/14514 , A61B5/14532 , A61B5/1455 , A61B5/1486 , A61B5/411 , A61B10/0064 , A61B2010/008 , A61B2017/00765 , A61B2562/0295 , C12Q1/54 , G01N33/66
摘要： Methods for detecting the presence or amount of an analyte present beneath a target skin or mucosal surface of an individual are provided. The methods entail disruption of the target skin or mucosal surface, for example using a particle delivery method to provide micro-passages in the tissue. The methods further provide a resealable occlusive dressing or patch for protecting the target site from outside agents as well as maintaining hydration of the sample area. Maintaining hydration over the sampling site allows for continuous diffusion of the analyte of interest from beneath the target site to the target site. Multiple samples over time may then be taken, allowing the user to monitor for the presence of analyte over time. In a preferred embodiment, the methods are used to monitor blood glucose levels. Figure 1 is a perspective view of resealable, occlusive dressing, with an aperture cover in a closed position.
摘要翻译：提供了用于检测存在于个体的目标皮肤或粘膜表面下方的分析物的存在或量的方法。该方法需要破坏目标皮肤或粘膜表面，例如使用颗粒递送方法在组织中提供微通道。该方法进一步提供了用于保护靶位点免受外界因子影响以及维持样品区域水合作用的可重新密封的封闭敷料或贴剂。保持采样点上的水合可以使目标分析物从目标点下方连续扩散到目标点。随后可以采集多个样品，允许用户随时监测分析物的存在。在一个优选的实施方案中，该方法用于监测血糖水平。图1是可重新密封的封闭敷料的透视图，其中孔盖处于关闭位置。

66. 发明公开

EP1445329A2 Method and test strip for determining glucose in blood 审中-公开
标题翻译：方法和Teststreifen zur Bestimmung von Glukose在Blut
公开(公告)号：EP1445329A2
公开(公告)日：2004-08-11
申请号：EP04001906.9
申请日：2004-01-29
申请人： Bayer HealthCare, LLC
发明人： Marfurt, Karen L.
IPC分类号： C12Q1/54 , G01N33/52
CPC分类号： A61B5/1486 , A61B5/14532 , A61B2562/0295 , C12Q1/54 , C12Q2326/00 , G01N33/66 , Y10T436/144444
摘要： A reagent strip for measuring the concentration of glucose in whole blood has a polymer on at least one side thereof to increase the opacity thereof, thereby reducing the effect of hematocrit on the glucose determination.
摘要翻译：用于测量全血中葡萄糖浓度的试剂条在其至少一侧具有聚合物以增加其不透明度，从而降低血细胞比容对葡萄糖测定的影响。

67. 发明公开

EP1434872A1 BBC3 GENE PROMOTER AND METHODS FOR IDENTIFYING MODULATORS OF APOPTOSIS AND BBC3 GENE EXPRESSION USING A BBC3 GENE PROMOTER 审中-公开
标题翻译： BBC3基因启动子和一种用于识别凋亡和BBC3基因表达的调节剂使用一个BBC3基因启动子
公开(公告)号：EP1434872A1
公开(公告)日：2004-07-07
申请号：EP02742258.3
申请日：2002-07-10
申请人： Immunogen, Inc.
发明人： HAN, Jia-Wen , CHITTENDEN, Thomas
IPC分类号： C12Q1/10 , C12Q1/42 , C12Q1/48 , C12Q1/54 , C12Q1/66 , C12Q1/68 , G01N33/53
CPC分类号： G01N33/5023 , G01N33/5008 , G01N33/5011 , G01N2500/20 , G01N2510/00
摘要： The present invention is directed to methods for identifying compounds that modulate apoptosis, directly or indirectly, as measured by changes in expression of the bbc3 gene or a reporter gene, or by changes in the apoptotic character of a cell. The present invention is also directed to methods for identifying compounds that modulate expression of the bbc3 gene and a method for identifying compounds that interact with a promoter of the bbc3 gene. The present invention is further directed to a method for modulating apoptosis in a cell, a method for modulating bbc3 gene expression in a cell, a process for making a modulator of apoptosis, and a process for making a modulator of bbc3 gene expression. Finally, the present invention is directed to compounds identified by each of the methods of this invention, and to promoters of the bbc3 gene and sequences homologous thereto.

68. 发明公开

EP1373506A2 HEFESTAMM VON SACCHAROMYCES CEREVISIAE MIT FUNKTIONELLER EXPRESSION EINES GLUT-TRANSPORTERS 有权
标题翻译：含有A GLUT转运体功能性表达酿酒酵母的酵母菌株
公开(公告)号：EP1373506A2
公开(公告)日：2004-01-02
申请号：EP02719792.0
申请日：2002-02-09
申请人： Aventis Pharma Deutschland GmbH
发明人： MÜLLER, Günter , KOLLER, Klaus-Peter , BOLES, Eckhard , WIECZORKE, Roman , DLUGAI, Silke
IPC分类号： C12N15/12 , C12N15/81 , C12N1/18 , C07K14/62 , C12Q1/02 , C12Q1/54
CPC分类号： C12R1/865 , C12N1/18
摘要： The invention relates to a yeast strain of Saccharomyces cerevisiae which, due to deletion of the genomic sequences, does not produce any hexose transporters and which as a consequence cannot propagate on substrates that have hexose as the only carbon source. The capability of the strain of propagating on substrates that have hexose as the only carbon source can be re-established by expression of a GLUT4 gene.

69. 发明公开

EP1342790A2 REAGENT AND METHOD FOR MEASURING AMYLASE ACTIVITY 有权
标题翻译： Methode zur Messung von淀粉酶Aktivität
公开(公告)号：EP1342790A2
公开(公告)日：2003-09-10
申请号：EP03005163.5
申请日：2003-03-07
申请人： Toyama University , Nipro Corporation , YAMAHA HATSUDOKI KABUSHIKI KAISHA
发明人： YOSHIDA, Hiroshi , KUSABA, Nobutaka , MIZUNO, Yasufumi
IPC分类号： C12Q1/40 , C12Q1/54 , G01N33/48
CPC分类号： C12Q1/40 , C12Q1/54
摘要： An object of the present invention is to provide a means for the measurement of amylase activity that exists in a biological sample such as saliva in a more convenient manner and particularly to provide a means (method and reagent) where a sample containing amylase in high concentration is directly measured without dilution. In the present invention, there has been found a method, which is an enzymatic method using a modified oligosaccharide substrate, comprising adding saccharide such as oligosaccharide that is competitive to the oligosaccharide substrate whereupon amylase activity in an amylase sample having a high activity value can be directly measured without dilution, and a result, the present invention has been achieved.
摘要翻译：本发明的目的是提供一种用于以更方便的方式测量存在于生物样品如唾液中的淀粉酶活性的方法，特别是提供一种方法（方法和试剂），其中含有高浓度淀粉酶的样品直接测量而不稀释。在本发明中，已经找到了使用改性寡糖底物的酶法的方法，其包括向寡糖底物添加竞争性低聚糖等糖类，由此具有高活性值的淀粉酶样品中的淀粉酶活性可以为没有稀释直接测量，结果已经实现了本发明。

70. 发明授权

EP0760003B1 SIMULTANEOUS ENUMERATION OF E. COLI AND TOTAL COLIFORMS 失效
标题翻译：大肠杆菌和所有法同时计数FORMS COLI
公开(公告)号：EP0760003B1
公开(公告)日：2003-09-03
申请号：EP95917174.5
申请日：1995-04-26
申请人： The Regents of the University of California
发明人： CHANG, George University of California, Berkeley , LUM, Rosalind University of California, Berkeley
IPC分类号： C12Q1/02 , C12Q1/04 , C12Q1/06 , C12Q1/10 , C12Q1/34 , C12Q1/54 , C12N1/20
CPC分类号： C12Q1/10 , G01N2333/245
摘要： The general method involves contacting a bacterial colony with a beta -D-galactosidase substrate and a carbon source (e.g. adonitol, esculin, salicin, amygdalin, cellobiose, etc.) metabolizable by a plurality of coliform species but not metabolizable by E. coli, and detecting reaction product signals of the substrate and carbon source. The absence of both reaction product signals indicates the presence of non-coliform bacteria; the presence of both reaction product signals indicates the presence of non-E. coli coliform bacteria; and the presence of the beta -D-galactosidase substrate reaction product signal and absence of the carbon source reaction product signal indicates the presence of E. coli. A signal may identify a reaction product per se (e.g. a colored product derived from a chromogenic form of the substrate or from a chromogenic reagent which interacts with the product, etc.), or an effect of a reaction product on or at the colony (e.g. catabolite repression, growth, localized pH change, etc.). In a preferred embodiment, the beta -D-galactosidase substrate reaction product signal is provided directly by a beta -D-galactosidase reaction product and the carbon source reaction product signal is provided by carbon source reaction product-induced catabolite repression of the beta -D-galactosidase substrate reaction product signal at a portion of the colony.

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