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61. 发明公开

EP0665754A4 ANTIMICROBIAL PEPTIDES. 失效
标题翻译：抗微生物多肽。
公开(公告)号：EP0665754A4
公开(公告)日：1996-06-12
申请号：EP93923208
申请日：1993-10-01
申请人： UNIV EMORY , UNIV GEORGIA RES FOUND
发明人： SHAFER WILLIAM , POHL JAN
IPC分类号： A61K38/00 , A01N37/46 , A61P1/02 , A61P31/04 , C07K7/06 , C07K7/08 , C07K14/435 , C07K14/46 , C07K14/47 , C12N9/64 , C12N9/76 , C07K14/00
CPC分类号： C07K7/06 , A61K38/00 , C07K7/08 , C12N9/64 , C12N9/6424 , C12N9/6427
摘要： Peptides which exhibit antimicrobial activity comparable to certain known antibiotics are provided. These peptides are related in sequence to amino acid sequences within Cathepsin G. A broad spectrum bactericidal peptide disclosed herein is RPGTLCTVAGWGRVSMRRGT (SEQ ID NO:22). It is active against Pseudomonas aeruginosa, Neisseria gonorrhoeae and Staphylococcus aureus. RRENTQQHITARRAIRHPQY (SEQ ID NO:19) and GKSSGVPPEVFTRFVSSFLPWIRTTMR (SEQ ID NO:26) also exhibited potent activity against P. aeruginosa strains, including clinical isolates. IIGGR (SEQ ID NO:1) and IVGGR (SEQ ID NO:2) act against both gram-negative and gram-positive bacterial strains. HPQYNQR (SEQ ID NO:3) and certain related peptides are also active against both gram-negative and gram-positive bacteria, including, but not limited to, strains of Escherichia coli, Neisseria gonorrhoeae, Staphylococcus aureus, Capnocytophage sputigena and Pseudomonas aeruginosa. The peptides of the present invention will be useful in pharmaceutical compositions useful in the treatment of prophylaxis of infections.

62. 发明公开

EP0511221A1 VERFAHREN ZUR ANREICHERUNG BZW. REINIGUNG VON BIOMOLEKÜLEN 失效
标题翻译： PROCESS FOR富集或清洁水蛭素。
公开(公告)号：EP0511221A1
公开(公告)日：1992-11-04
申请号：EP91900759.0
申请日：1990-12-20
申请人： BASF Aktiengesellschaft
发明人： LANG, Kurt , Kreimeyer, Andreas
IPC分类号： C12N15 , C07D475 , C07K1 , C07K14 , C12N9 , C12P21 , C12R1
CPC分类号： C12N9/6427 , C07D475/04 , C07K1/30 , C07K14/815 , C12N9/00 , Y10S530/855
摘要： Il est décrit un procédé pour l'enrichissement ou la purification de biomolécules consistant ce que l'on introduit la biomolécule dans un système de solvants organiques aqueux et que l'on provoque par l'adjonction de sels une séparation de phase. A cette occasion, la valeur du pH à laquelle a lieu l'enrichissement, les solvants appropriés aux système de solvants organiques aqueux et le type de sel sont déterminés au cours d'une série d'essais.

63. 发明授权

EP3052623B1 HUMAN TRYPSINOGEN WITH REDUCED AUTOACTIVATION AND ITS USE IN AN IMMUNOASSAY 有权
标题翻译：减少自体激活的人胰蛋白酶原及其在免疫测定中的应用
公开(公告)号：EP3052623B1
公开(公告)日：2018-02-21
申请号：EP14781104.6
申请日：2014-09-25
申请人： Life Science Inkubator GmbH Bonn
发明人： BÜTTNER, Karin , PRASSE, Agneta , ZÜCHNER, Thole
IPC分类号： C12N9/76 , C12Q1/37 , G01N33/542 , G01N33/58
CPC分类号： C12N9/6427 , C12Q1/37 , C12Y304/21004 , G01N33/542
摘要： The present invention relates to a polypeptide consisting of or comprising a variant of human trypsinogen-1, wherein the variant is characterized in that an amino acid residue selected from A16, A17, K64, K138, N139, L142 and L147 is replaced by an amino acid residue with a negatively charged side chain and/or H18 is replaced by a glutamine or asparagine residue and/or L107 is replaced by an amino acid residue with a positively charged side chain and/or N139 is replaced by an amino acid residue with an aromatic side chain, wherein the variant is cleavable into a polypeptide having a native-like enzymatic activity when compared to human trypsin-1. The present invention further relates to means and method for manufacturing the polypeptide as well as the use of the polypeptide, particularly in an immunoassay.

64. 发明公开

EP2857507A1 Human trypsinogen with reduced autoactivation and its use in an immunoassay 审中-公开
标题翻译： Menschliches胰蛋白酶抑制剂Autoaktivierung und dessen Verwendung in einem Immuntest
公开(公告)号：EP2857507A1
公开(公告)日：2015-04-08
申请号：EP13187194.9
申请日：2013-10-02
申请人： Universität Leipzig
发明人： Büttner, Karin , Prasse, Agneta , Züchner, Thole
IPC分类号： C12N9/76 , C12Q1/37 , G01N33/542 , G01N33/58
CPC分类号： C12N9/6427 , C12Q1/37 , C12Y304/21004 , G01N33/542
摘要： The present invention relates to a polypeptide consisting of or comprising a variant of human trypsinogen-1, wherein the variant is characterized in that an amino acid residue selected from A16, A17, K64, K138, N139, L142 and L147 is replaced by an amino acid residue with a negatively charged side chain and/or H18 is replaced by a glutamine or asparagine residue and/or L107 is replaced by an amino acid residue with a positively charged side chain and/or N139 is replaced by an amino acid residue with an aromatic side chain, wherein the variant is cleavable into a polypeptide having a native-like enzymatic activity when compared to human trypsin-1. The present invention further relates to means and method for manufacturing the polypeptide as well as the use of the polypeptide, particularly in an immunoassay.
摘要翻译：本发明涉及由胰蛋白酶原-1变体组成或包含人胰蛋白酶原-1变体的多肽，其特征在于，选自A16，A17，K64，K138，N139，L142和L147的氨基酸残基被氨基酸具有带负电荷侧链和/或H18的酸残基被谷氨酰胺或天冬酰胺残基替代和/或L107被具有带正电荷侧链的氨基酸残基替代，和/或N139被氨基酸残基替换，芳族侧链，其中当与人胰蛋白酶-1相比时，该变体可切割成具有天然样酶活性的多肽。本发明还涉及用于制备多肽的方法和方法以及多肽的使用，特别是在免疫测定中。

65. 发明公开

EP2413720A1 PROCESS FOR MAKING A MILK-BASED PROTEIN HYDROLYSATE 有权
标题翻译： VERFAHREN ZUR HERSTELLUNG EINES蛋白质蛋白酶AUF MILCHBASIS
公开(公告)号：EP2413720A1
公开(公告)日：2012-02-08
申请号：EP10715146.6
申请日：2010-03-31
申请人： Novozymes A/S
发明人： OESTERGAARD, Peter R. , ERNST, Steffen , LYNGLEV, Gitte, B.
IPC分类号： A23L1/305 , A61K38/01 , C12N9/52 , C12N9/58 , C12N9/64 , C12N9/76
CPC分类号： A23L33/18 , A23L33/19 , A23L33/40 , C12N9/52 , C12N9/58 , C12N9/6424 , C12N9/6427
摘要： The present invention relates to an enzymatic process for making a milk-based protein hydrolysate and use of such hydrolysate, e.g., in an infant formula composition.
摘要翻译：本发明涉及用于制备基于乳的蛋白质水解产物的酶法和使用这种水解产物，例如在婴儿配方食品组合物中。

66. 发明授权

EP1926749B1 CLEAVAGE OF PRECURSORS OF INSULINS BY A VARIANT OF TRYPSIN 有权转让
标题翻译：胰岛素前体由胰蛋白酶SPLIT
公开(公告)号：EP1926749B1
公开(公告)日：2011-07-27
申请号：EP06791678.3
申请日：2006-08-26
申请人： Sanofi-Aventis Deutschland GmbH , F.Hoffmann-La Roche AG , Roche Diagnostics GmbH
发明人： GEIPEL, Frank , GLASER, Stephan , HOH, Christoph , KOPETZKI, Erhard , MUELLER, Rainer , ZOCHER, Frank
IPC分类号： C07K14/81
CPC分类号： C07K14/62 , C12N9/6427
摘要： The present invention relates to the production of a variant of recombinant trypsin with increased substrate specificity for arginine versus lysine in non-animal host organisms. Moreover, the present invention relates to a variant of recombinant trypsin and their production. Also provided are use of recombinant porcine pancreatic trypsin variants for cleavage of precursors of insulins, and kits containing the variant of trypsin.

67. 发明授权

EP1678296B1 PROTEASE WITH IMPROVED STABILITY IN DETERGENTS 有权
标题翻译：稳定性改进洗涤剂蛋白酶
公开(公告)号：EP1678296B1
公开(公告)日：2011-07-13
申请号：EP04790060.0
申请日：2004-10-22
申请人： Novozymes A/S
发明人： WU, Wenping, Er Dan Yuan, Bldg 3, Room 13 , JOKUMSEN, Kirsten, Vaever , STRINGER, Mary, Ann
IPC分类号： C12N9/58 , C12N9/76 , C11D3/386
CPC分类号： C12N9/6427 , C11D3/386 , C12N9/58
摘要： The present invention relates to proteases having improved stability in detergent compositions. Further, the present invention relates to cleaning and detergent compositions comprising the proteases of the invention as well as to use of such protease in detergent compositions.

68. 发明授权

EP1546302B1 METHODS FOR PRODUCING MAMMALIAN TRYPSINS 有权
标题翻译：用于生产哺乳动物胰蛋白酶
公开(公告)号：EP1546302B1
公开(公告)日：2009-08-12
申请号：EP03755759.2
申请日：2003-08-29
申请人： Novozymes, Inc.
发明人： BERKA, Randy , BROWN, Kimberly
IPC分类号： C12N9/76 , C12N1/15 , C12N15/52 , C12N15/62 , C12P21/02
CPC分类号： C12N9/6427 , C12N9/6421 , C12N15/80 , C12Y304/21004
摘要： The present invention relates to methods for producing a mammalian trypsin in a Fusarium venenatum host strain, comprising (a) cultivating the Fusarium venenatum host strain in a culture medium under conditions suitable for expression of the mammalian trypsin and secretion thereof into the medium, wherein the Fusarium venenatum host strain comprises a nucleic acid construct comprising a nucleic acid sequence encoding the mature coding sequence of a mammalian trypsin operably linked to nucleotides 58 to 129 of SEQ ID NO: 1 encoding the signal peptide and propeptide of Fusarium oxysporum trypsinogen; and (b) recovering the mammalian trypsin from the medium. The present invention also relates to constructs comprising a nucleic acid sequence encoding the mature coding sequence of a mammalian trypsin operably linked to nucleotides 58 to 129 of SEQ ID NO: 1 encoding the signal peptide and propeptide of Fusarium oxysporum trypsinogen, and to vectors and Fusarium venenatum host strains comprising such constructs.

69. 发明授权

EP1778839B1 C-TERMINAL MODIFICATION OF POLYPEPTIDES 有权转让
标题翻译：多肽的C-端修饰
公开(公告)号：EP1778839B1
公开(公告)日：2008-07-09
申请号：EP05774367.6
申请日：2005-08-12
申请人： Roche Diagniostics GMBH , F.HOFFMANN-LA ROCHE AG
发明人： HOESS, Eva , BORDUSA, Frank , HERRMANN, Rupert , JAKUBKE, Hans-Dieter , TISCHER, Wilhelm
IPC分类号： C12N9/76 , C12Q1/37 , C12N15/10
CPC分类号： C12N9/6424 , C12N9/6427 , C12P21/06
摘要： The invention relates to a mutated trypsin comprising an amino acid substitution both at position K60 and D189, and at least one more amino acid substitution by histidine at position N143 or position E151. Such trypsin mutant has a preferred cleavage site comprising the amino acids Xaal-Xaaz His, wherein Xaal is L, Y or F and Xaa2 is R or K. The invention also relates to a man-made polypeptide comprising a target peptide and the above cleavage site as well as to a method of producing C-terminally modified target peptides by using this mutated trypsin.

70. 发明公开

EP1868989A2 INHIBITORS OF NEUROTRYPSIN AND DETERMINATION THEREOF 审中-公开
标题翻译：抑制剂并决心于神经
公开(公告)号：EP1868989A2
公开(公告)日：2007-12-26
申请号：EP06725408.6
申请日：2006-03-29
申请人： UNIVERSITY OF ZURICH
发明人： SONDEREGGER, Peter , HETTWER, Stefan , BOLLIGER, Marc, F. , DREIER, Birgit , KUNZ, Beat , LÜSCHER, Daniel , REIF, Raymond , SALES, Susanne
IPC分类号： C07C311/47 , A61K31/63 , A61P25/18
CPC分类号： C12N9/6427 , C07C311/64 , C12Q1/37 , G01N2333/976 , G01N2500/02
摘要： The invention relates to a method for determining whether a compound is a neurotrypsin inhibitor, characterized in that the compound is incubated together with neurotrypsin, a variant thereof or a fragment comprising the protease domain and with a protein or peptide comprising agrin, a variant thereof or a fragment comprising the - or the - cleavage site of agrin, in an aqueous buffer solution, and the amount of cleavage of agrin is measured. Additionally, the invention relates to inhibitors of neurotrypsin found by this method, in particular to compounds of formula (1) wherein Hal
1 and Hal
2 are fluorine, chlorine or bromine, and the use of such inhibitors for the treatment and/or prophylaxis of diseases caused by deficiency of synapses, for example skeletal muscle atrophy, schizophrenia, and cognitive disturbance.

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