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41. 发明授权

EP2374900B1 POLYNUCLEOTIDES FOR THE AMPLIFICATION AND ANALYSIS OF WHOLE GENOME AND WHOLE TRANSCRIPTOME LIBRARIES GENERATED BY A DNA POLYMERIZATION PROCESS 有权转让
标题翻译：用于扩增和GESAMTGENOM- AND GESAMTTRANSKRIPTOM图书馆分析多核苷酸通过DNA聚合产生WERE
公开(公告)号：EP2374900B1
公开(公告)日：2016-07-13
申请号：EP10011482.6
申请日：2004-03-08
申请人： Rubicon Genomics, Inc.
发明人： Kamberov, Emmanuel , Sun, Tong , Bruening, Eric E. , Pinter, John , Sleptsova, Irina , Kurihara, Takao , Makarov, Vladimir, L.
IPC分类号： C40B40/06 , C40B50/06 , C12Q1/68 , C12N15/10
CPC分类号： C12N15/1093 , C12N15/10 , C12Q1/686 , C40B40/06 , C40B50/06 , C12Q2525/161 , C12Q2525/179 , C12Q2525/15

42. 发明公开

EP3027766A1 SEQUENCE CAPTURE METHOD USING SPECIALIZED CAPTURE PROBES (HEATSEQ) 审中-公开
标题翻译：序列检测方法使用专门的捕获探针（HEATSEQ）
公开(公告)号：EP3027766A1
公开(公告)日：2016-06-08
申请号：EP14745144.7
申请日：2014-07-31
申请人： F. Hoffmann-La Roche AG , Roche Diagnostics GmbH
发明人： ALBERT, Thomas , NORTON, Jason , PATEL, Jigar , BURGESS, Daniel , LYAMICHEV, Victor , BROCKMAN, Michael
IPC分类号： C12Q1/28
CPC分类号： C12Q1/6876 , C12Q1/6813 , C12Q1/6874 , C12Q2525/161 , C12Q2525/179 , C12Q2537/159 , C12Q2563/179
摘要： The present invention is a novel protocol for the massively parallel production of improved MIPs. The molecular improvements to the MIP cover the manufacturing of the probes, the workflow, the addition of unique sequence elements which connote sample specificity, and a sequence tag which uniquely identifies a specific molecule present in the initial sample population. Lastly, this invention also is combined with an empirical optimization strategy that overcomes issues of both locus representation and allelic bias. This improved technique is scalable and can be utilized to amplify targets comprised of a single locus' amplicon up to targeting more than 1 million loci.

43. 发明公开

EP2824180A4 METHOD FOR DETECTING TARGET NUCLEIC ACID 审中-公开
标题翻译：一种用于检测靶核酸
公开(公告)号：EP2824180A4
公开(公告)日：2016-03-02
申请号：EP12870538
申请日：2012-11-15
申请人： NGK INSULATORS LTD
发明人： NIWA KOUSUKE , HIROTA TOSHIKAZU
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6837 , C12Q1/689 , C12Q2600/16 , C12Q2525/161 , C12Q2537/162 , C12Q2565/518
摘要： The disclosure of this Description includes: a step of preparing a solid-phase body provided with a detection probe having a predetermined nucleotide sequence; a step of preparing a signaling target nucleic acid having a signal generating part for detecting the target nucleic acid; a step of bringing the signaling target nucleic acid, the detection probe, and an oligonucleotide being a mediator having a first site that hybridizes specifically with the target nucleic acid and a second site (preferably 20 to 50 nucleotides or more preferably 20 to 25 nucleotides in length) that hybridizes specifically with a part having the predetermined nucleotide sequence of the detection probe into contact with one another so that a hybridization product of these components can be formed; and a step of obtaining data based on the signal generating part of the signaling target nucleic acid on the solid-phase body.

44. 发明授权

EP2531618B1 METHOD TO DETECT REPEAT SEQUENCE MOTIFS IN NUCLEIC ACID 有权转让
标题翻译：方法重复序列模体在DNA检测
公开(公告)号：EP2531618B1
公开(公告)日：2016-01-20
申请号：EP11740443.4
申请日：2011-02-04
申请人： Quest Diagnostics Investments Incorporated
发明人： HANTASH, Feras , SUN, Weimin , TSAO, David, C. , ROOT, Dana, Marie , STROM, Charles, M.
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6883 , C12Q1/6827 , C12Q1/683 , C12Q1/6858 , C12Q2600/154 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , G06F19/10 , C12Q2525/151 , C12Q2525/155 , C12Q2525/161 , C12Q2537/16

45. 发明公开

EP2841598A4 METHOD FOR COMPETITIVE ALLELE-SPECIFIC CDNA SYNTHESIS AND DIFFERENTIAL AMPLIFICATION OF THE CDNA PRODUCTS 失效
标题翻译： VERFAHRENFÜRKONKURRENZFÄHIGEALLELSPEZIFISCHE CDNA-SYNTHESE UND DIFFERENZIELLE AMPLIFATIONATION DER CDNA-PRODUKTE
公开(公告)号：EP2841598A4
公开(公告)日：2016-01-13
申请号：EP13781361
申请日：2013-04-25
申请人： HO THO HUU
发明人： HO THO HUU
IPC分类号： C12Q1/68 , C12N15/11
CPC分类号： C12Q1/6858 , C12Q2521/107 , C12Q2525/155 , C12Q2525/161 , C12Q2531/113 , C12Q2537/161
摘要： The present invention provides a method for the detection of the presence of RNA variants comprising the step of performing a competitive cDNA synthesis comprising a first primer specific to a first RNA variant, a second primer specific to a second RNA variant, an RNA-dependent DNA polymerase, and RNA from said sample as a template, wherein said first primer and said second primer comprise an allele-specific nucleotide portion, a target-specific sequence and tag units with a common sequence and/or a discriminating sequence so that the sequence of said tag units is not complementary to said first or second RNA variant, wherein each of the cDNA products obtained from said competitive cDNA synthesis consists of the sequence of only one primer extended by the sequence complementary to one of the target RNA variants.
摘要翻译：本发明提供了检测RNA变体存在的方法，其包括进行竞争性cDNA合成的步骤，其包括对第一RNA变体特异性的第一引物，对第二RNA变体特异性的第二引物，RNA依赖性DNA 聚合酶和RNA作为模板，其中所述第一引物和所述第二引物包含等位基因特异性核苷酸部分，靶特异性序列和具有共同序列和/或鉴别序列的标签单位，使得序列所述标签单元与所述第一或第二RNA变体不互补，其中从所述竞争性cDNA合成获得的每种cDNA产物由仅与一种靶RNA变体互补的序列延伸的一个引物的序列组成。

46. 发明公开

EP2836611A4 DETECTION AND QUANTITATION OF SAMPLE CONTAMINATION IN IMMUNE REPERTOIRE ANALYSIS 审中-公开
标题翻译：美国国际药物管理局在欧洲免疫缺陷病毒
公开(公告)号：EP2836611A4
公开(公告)日：2015-12-30
申请号：EP13775514
申请日：2013-04-09
申请人： SEQUENTA INC
发明人： ASBURY THOMAS , CARLTON VICTORIA , FAHAM MALEK , MACEVICZ STEPHEN , HEAD MARTIN , WILLIS THOMAS , ZHENG JIANBIAO
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , C12Q2525/161 , C12Q2535/122
摘要： The invention is directed to methods for detecting and quantifying nucleic acid contamination in a tissue sample of an individual containing T cells and/or B cells, which is used for generating a sequence-based clonotype profile. In one aspect, the invention is implemented by measuring the presence and/or level of an endogenous or exogenous nucleic acid tag by which nucleic acid from an intended individual can be distinguished from that of unintended individuals. Endogenous tags include genetic identity markers, such as short tandem repeats, rare clonotypes or the like, and exogenous tags include sequence tags employed to determine clonotype sequences from sequence reads.
摘要翻译：本发明涉及用于检测和定量含有T细胞和/或B细胞的个体的组织样品中的核酸污染的方法，其用于产生基于序列的克隆型谱。在一个方面，本发明通过测量内源或外源核酸标签的存在和/或水平来实现，通过该核酸标签，来自预期个体的核酸可以与非预期个体的核酸区别。内源性标签包括遗传同一性标记，例如短串联重复，罕见克隆型等，外源标签包括用于从序列读数确定克隆型序列的序列标签。

47. 发明公开

EP2859123A4 METHOD OF SEQUENCE DETERMINATION USING SEQUENCE TAGS 审中-公开
标题翻译：顺序确定顺序的程序
公开(公告)号：EP2859123A4
公开(公告)日：2015-12-16
申请号：EP13804085
申请日：2013-06-11
申请人： SEQUENTA INC
发明人： FAHAM MALEK , MOORHEAD MARTIN , WILLIS THOMAS , ZHENG JIANBIAO
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6869 , C12Q2565/514 , C12Q2565/537 , C12Q2525/161 , C12Q2525/179 , C12Q2525/191

48. 发明授权

EP2710145B1 METHODS AND COMPOSITIONS FOR DETECTING TARGET NUCLEIC ACIDS 有权
标题翻译：方法和组合物靶核酸的检测
公开(公告)号：EP2710145B1
公开(公告)日：2015-12-09
申请号：EP12723799.8
申请日：2012-05-17
申请人： Dxterity Diagnostics Incorporated
发明人： TERBRUEGGEN, Robert , LIU, Yenbou , CHILDERS, John, Ray , KIM, Chang, Hee , ABEDI, Majid R.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6813 , C12Q1/6809 , C12Q1/6834 , C12Q1/6855 , C12Q1/6862 , C12Q2523/109 , C12Q2525/155 , C12Q2525/161 , C12Q2525/197 , C12Q2525/204 , C12Q2533/107 , C12Q2537/143 , C12Q2563/131 , C12Q2563/179 , C12Q2565/125 , C12Q2525/15
摘要： The present invention provides compositions, apparatuses and methods for detecting one or more nucleic acid targets present in a sample. Methods of the invention include utilizing two or more ligation probes that reversibly bind a target nucleic acid in close proximity to each other and possess complementary reactive ligation moieties. When such probes have bound to the target in the proper orientation, they are able to undergo a spontaneous chemical ligation reaction that yields a ligation product that is directly detected or that is amplified to produce amplicons that are then detected. The present invention also provides methods to stabilize sample RNA so that degradation does not significantly affect the results of the analysis.

49. 发明公开

EP2949759A1 Multiplexed homogeneous oligonucleotide detection 有权
标题翻译：多重均质寡核苷酸检测
公开(公告)号：EP2949759A1
公开(公告)日：2015-12-02
申请号：EP14305794.1
申请日：2014-05-27
申请人： UNIVERSITE PARIS-SUD 11 , Centre National de la Recherche Scientifique , Lumiphore, Inc.
发明人： Hildebrandt, Niko , Jin, Zongwen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , G01N21/6428 , G01N2021/6439 , C12Q2525/161 , C12Q2525/207 , C12Q2533/107
摘要： The present invention concerns a new kit for detecting or quantifying the amount of one or multiple nucleic acid targets in a biological sample, and a detection or quantification method using this kit. Said kit is constituted by a universal energy donor probe, a universal energy acceptor probe and a couple of adaptors.
摘要翻译：本发明涉及用于检测或定量生物样品中一个或多个核酸靶标的量的新型试剂盒以及使用该试剂盒的检测或定量方法。所述试剂盒由通用能量供体探针，通用能量受体探针和一对衔接头构成。

50. 发明公开

EP2823303A4 MOLECULAR DIAGNOSTIC SCREENING ASSAY 审中-公开
标题翻译：分子诊断检测
公开(公告)号：EP2823303A4
公开(公告)日：2015-09-30
申请号：EP13746127
申请日：2013-02-11
申请人： RAINDANCE TECHNOLOGIES INC
发明人： WATSON ANDREW , LINK DARREN ROY , OLSON JEFFREY CHARLES , SAMUELS MICHAEL
IPC分类号： G01N33/53 , C07H21/00 , C12Q1/68
CPC分类号： C12N15/1072 , C12Q1/6827 , C12Q1/6883 , C12Q1/6886 , C12Q2600/156 , C12Q2525/155 , C12Q2525/161 , C12Q2563/159 , C12Q2563/179 , C12Q2565/629
摘要： The invention generally relates to method for screening for a condition in a subject. In certain embodiments, methods of the invention involve obtaining a pool of nucleic acids from a sample, incubating the nucleic acids with first and second sets of binders, in which the first set binds uniquely to different regions of a target nucleic acid in the pool, the second set binds uniquely to different regions of a reference nucleic acid in the pool, and the first and second sets include different detectable labels, removing unbound binders, detecting the labels, and screening for a condition based upon results of the detecting step.

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