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11. 发明公开

EP1152054A1 NOVEL CARBONYL REDUCTASE, GENE THEREOF AND METHOD OF USING THE SAME 有权
标题翻译： NEER CARBONYL-REDUKTASE，GEN DAVON UND VERFAHREN DER ANWENDUNG DERSELBEN
公开(公告)号：EP1152054A1
公开(公告)日：2001-11-07
申请号：EP00977938.0
申请日：2000-11-24
申请人： Kaneka Corporation
发明人： KIZAKI, Noriyuki , YAMADA, Yukio , YASOHARA, Yoshihiko , HASEGAWA, Junzo
IPC分类号： C12N9/02 , C12N15/53 , C12N9/04 , C12N1/21 , C12P7/62
CPC分类号： C12N9/0006 , C12P7/62 , C12P41/00 , C12P41/002
摘要： A novel polypeptide for producing tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate, a gene encoding the polypeptide, and a method of using the polypeptide, are provided. The polypeptide has an enzyme activity to asymmetrically reduce tert-butyl (S)-6-chloro-5-hydroxy-3-oxohexanoate to tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate. The polypeptide comprises the amino acid sequence represented by SEQ ID NO. 1 in the Sequence Listing, or the amino acid sequence having at least one amino acid substitution, insertion, deletion, or addition thereof.
摘要翻译：提供了一种用于制备（3R，5S）-6-氯-3,5-二羟基己酸叔丁酯，编码该多肽的基因以及使用该多肽的方法的新型多肽。该多肽具有不对称地将（S）-6-氯-5-羟基-3-氧代己酸叔丁酯还原成（3R，5S）-6-氯-3,5-二羟基己酸叔丁酯的酶活性。多肽包含SEQ ID NO：1所示的氨基酸序列。或者具有至少一个氨基酸取代，插入，缺失或添加的氨基酸序列。

12. 发明公开

EP0987332A1 PROCESS FOR PRODUCING OPTICALLY ACTIVE AMINO COMPOUNDS 失效
标题翻译： Deoxynukleinsäure编码具有立体选择性转氨酶活性，和含有该Deoxynukleinsäure转化的多肽
公开(公告)号：EP0987332A1
公开(公告)日：2000-03-22
申请号：EP98914111.4
申请日：1998-04-20
申请人： Kaneka Corporation
发明人： YAMADA, Yukio , IWASAKI, Akira , KIZAKI, Noriyuki , MATSUMOTO, Keiji , IKENAKA, Yasuhiro , OGURA, Masahiro , HASEGAWA, Junzo
IPC分类号： C12P13/04 , C12N15/54
CPC分类号： C12P13/001 , C12N9/1096
摘要： A process for efficiently producing optically active amino compounds, mainly (R)-amino compounds, at a low cost by means of a microbial enzyme; a polypeptide suitable for use in the production process and having a stereoselective transaminase activity; and a DNA coding for the polypeptide. The process is characterized by comprising causing a transaminase to act on a ketone compound as an amino acceptor in the presence of a primary amine as an amino donor to stereoselectively transfer the amino group to thus obtain an optically active amino compound. The DNA contains a base sequence coding for a polypeptide having a stereoselective transaminase activity. The polypeptide can be obtained by incubating a microorganism belonging to the genus Arthrobacter .
摘要翻译：一种用于有效地制造光学活性氨基化合物，主要（R）的氨基化合物，在通过微生物酶的手段低成本过程; 适合于在生产过程中使用，并且具有立体选择性转氨酶活性的多肽; 和DNA编码的多肽。该过程由包括使转氨酶在伯胺的存在下在酮化合物作为氨基受体的作为氨基供体的立体选择性地转移的氨基，得到因而光学活性氨基化合物为特征的。的DNA含有的碱基序列编码具有立体选择性转氨酶活性的多肽。所述多肽可通过温育属于节杆菌属的微生物来获得。

13. 发明授权

EP1306438B1 NOVEL CARBONYL REDUCTASE, GENE THEREOF AND METHOD OF USING THE SAME 有权
标题翻译：新的羰基，基因和方法的使用
公开(公告)号：EP1306438B1
公开(公告)日：2008-06-25
申请号：EP01956776.7
申请日：2001-08-01
申请人： KANEKA CORPORATION
发明人： KIZAKI, Noriyuki , YASOHARA, Yoshihiko , HASEGAWA, Junzo
IPC分类号： C12N15/53 , C12N9/02 , C12N1/21 , C12P17/10
CPC分类号： C12N9/0008 , C12N9/0006 , C12P17/10 , C12P41/002
摘要： Novel polypeptides capable of forming (S)-N-benzyl-3-pyrrolidinol, DNA encoding the same and a method of using the same. A polypeptide having the following physicochemical properties (1) to (5): (1) function: asymmetrically reducing N-benzyl-3-pyrrolidinone by using NADPH as a coenzyme to form (S)-N-benzyl-3-pyrodinol; (2) optimum functional pH: 4.5 to 5.5; (3) optimum functional temperature: 40 to 45°C; (4) molecular weight: about 29,000 in gel filtration analysis, about 35,000 in SDS polyacrylamide electrophoresis; and (5) inhibitor: being inhibited by divalent copper ion. A polypeptide having the amino acid sequence represented by SEQ ID NO:1, or a polypeptide having an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO:1 by the substitution, insertion, deletion and/or addition of one or more amino acids and having an enzymatic activity of asymmetrically reducing N-benzyl-3-pyrrolidinone to form (S)-N-benzyl-3-pyrrolidinol.

14. 发明授权

EP0857790B1 PROCESS FOR PRODUCING OPTICALLY ACTIVE AMINO COMPOUNDS 失效
标题翻译：工艺生产光学活性的氨基酸债券
公开(公告)号：EP0857790B1
公开(公告)日：2005-08-17
申请号：EP96935392.9
申请日：1996-10-21
申请人： KANEKA CORPORATION
发明人： YAMADA, Yukio , IWASAKI, Akira , KIZAKI, Noriyuki , IKENAKA, Yasuhiro , OGURA, Masahiro , HASEGAWA, Junzo
IPC分类号： C12P13/00 , C12N9/10 , C12P41/00
CPC分类号： C12P13/001 , C12N9/1096 , C12P13/005 , C12P13/008 , C12P13/04 , C12P41/006 , Y10S435/83
摘要： A process for producing optically active (R)-amino compounds which comprises treating an aralkyl alkyl ketone compound (the amino receptor) coexisting with a racemic or (R)-amino compound (the amino donor) with an (R)-specific transaminase produced by a microorganism belonging to the genus Arthrobacter and thus effecting stereoselective transamination. This process makes it possible to facilitate the production in a high yield of optically active (R)-amino compounds, etc., having aryl, etc. at the 1-position which have been difficult to synthesize by the conventional techniques.

15. 发明授权

EP1152054B1 NOVEL CARBONYL REDUCTASE, GENE THEREOF AND METHOD OF USING THE SAME 有权
标题翻译：新羰基还原酶，其基因和给药方法相同
公开(公告)号：EP1152054B1
公开(公告)日：2005-03-23
申请号：EP00977938.0
申请日：2000-11-24
申请人： Kaneka Corporation
发明人： KIZAKI, Noriyuki , YAMADA, Yukio , YASOHARA, Yoshihiko , HASEGAWA, Junzo
IPC分类号： C12N9/02 , C12N15/53 , C12N9/04 , C12N1/21 , C12P7/62
CPC分类号： C12N9/0006 , C12P7/62 , C12P41/00 , C12P41/002
摘要： A novel polypeptide capable of forming tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate; and a polynucleotide encoding the same. Namely, a polypeptide which has an enzymatic activity of assymetrically reducing tert-butyl (S)-6-chloro-5-hydroxy-3-oxohexanoate to give tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate and contains the amino acid sequence represented by SEQ ID NO:1 in Sequence Listing or an amino acid sequence derived from the above amino acid sequence by the substitution, insertion, deletion or addition of at lease one amino acid.

16. 发明公开

EP1055732A1 PROCESS FOR PRODUCING (R)-2-HYDROXY-1-PHENOXYPROPANE DERIVATIVE 审中-公开
标题翻译：生产（R）-2-羟基-1-苯氧基丙烷衍生物的方法
公开(公告)号：EP1055732A1
公开(公告)日：2000-11-29
申请号：EP99959901.2
申请日：1999-12-20
申请人： KANEKA CORPORATION
发明人： TAOKA, Naoaki , KIZAKI, Noriyuki , YASOHARA, Yoshihiko , INOUE, Kenji , HASEGAWA, Junzo
IPC分类号： C12P7/22
CPC分类号： C12P7/22 , C12P41/002
摘要： The present invention is related to producing (R)-2-hydroxy-1-phenoxypropane derivatives, which are of value as intermediates of pharmaceuticals, with high optical purity and good efficiency,

comprising a step of reducing a phenoxyacetone derivative with a carbonyl reductase derived from a microorganism of the genus Candida or a culture broth or processed culture broth of said microorganism having the ability to elaborate the carbonyl reductase.
摘要翻译：本发明涉及生产具有高光学纯度和高效率的作为药物中间体的（R）-2-羟基-1-苯氧基丙烷衍生物，该方法包括用衍生的羰基还原酶还原苯氧基丙酮衍生物的步骤来自假丝酵母属的微生物或培养肉汤或所述微生物的加工过的培养肉汤，其能够精制所述羰基还原酶。

17. 发明公开

EP1024139A1 PROCESS FOR THE PREPARATION OF OPTICALLY ACTIVE 2- 6-(HYDROXYMETHYL)-1,3-DIOXAN-4-YL]ACETIC ACID DERIVATIVES 有权
标题翻译： VERFAHREN ZUR HERSTELLUNG OPTISCH AKTIVER 2-（6-（羟甲基）-1,3-DIOXAN-4-YL）-ESSIGSÄURE-DERIVATE
公开(公告)号：EP1024139A1
公开(公告)日：2000-08-02
申请号：EP99935066.3
申请日：1999-08-05
申请人： KANEKA CORPORATION
发明人： KIZAKI, Noriyuki , YAMADA, Yukio , YASOHARA, Yoshihiko , NISHIYAMA, Akira , MIYAZAKI, Makoto , MITSUDA, Masaru , KONDO, Takeshi , UEYAMA, Noboru , INOUE, Kenji
IPC分类号： C07D319/06 , C07D319/08 , C07C59/90 , C07C51/353 , C07C59/115 , C12P7/04
CPC分类号： C07D319/06 , C07B2200/07 , C07C67/343 , C07C253/30 , C12P7/62 , C07C69/716 , C07C255/21
摘要： This invention provides a process for producing optically active 2-[6-(hydroxymethyl)-1,3-dioxan-4-yl] acetic acid derivatives, which are of value as intermediates of drugs, from inexpensive starting materials without using any special equipment such as that required for super-low temperature reactions.
A process for producing optically active 2-[6-(hydroxymethyl)-1,3-dioxan-4-yl]acetic acid derivatives which comprises

reacting an acetic acid derivative at a temperature of not less than -30°C with an enolate prepared by permitting either a base or a metal having a valency of 0 to act on the derivative to produce a hydroxyoxohexanoic acid derivative,
reducing this compound with the aid of a strain of microorganism to provide a halomethyldioxanylacetic acid derivative,
treating this compound with an acetalizing agent in the presence of an acid catalyst to provide a halomethyldioxanylacetic acid derivative,
reacted with an acyloxylating agent to provide a acyloxymethyldioxanylacetic acid derivative, and
subjecting this compound to solvolysis in the presence of a base.
摘要翻译：本发明提供了一种从廉价起始原料生产具有价值作为药物中间体的光学活性2-δ-（羟甲基）-1,3-二恶烷-4-基乙酸衍生物的方法，而不使用任何特殊设备，例如这是超低温反应所需的。一种制备光学活性的2-δ-（羟甲基）-1,3-二恶烷-4-基乙酸衍生物的方法，其包括使不饱和-30℃的乙酸衍生物与通过使任一种碱或金属，其化合价为0，作用于衍生物以产生羟基己酸衍生物，借助微生物菌株还原该化合物，得到卤代甲基二恶烷基乙酸衍生物，在缩合剂存在下用缩醛化剂处理该化合物的酸催化剂，得到卤代甲基二恶烷基乙酸衍生物，与酰氧基化剂反应，得到酰氧基甲基二恶烷基乙酸衍生物，并在碱的存在下使其溶解。

18. 发明公开

EP0967271A1 NOVEL CARBONYL REDUCTASE, GENE THAT ENCODES THE SAME, AND METHOD OF UTILIZING THESE 失效
标题翻译：新羰基还原酶，基因，供他们使用此代码，方法
公开(公告)号：EP0967271A1
公开(公告)日：1999-12-29
申请号：EP97937861.9
申请日：1997-09-01
申请人： KANEKA CORPORATION
发明人： YASOHARA, Yoshihiko , KIZAKI, Noriyuki , HASEGAWA, Junzo , WADA, Masaru, Usuki-manshon 305 , SHIMIZU, Sakayu, 6-9, Tokiwayamashita-cho , KATAOKA, Michihiko, Haitsukosumo 227 , YAMAMOTO,K. Nihontabaccosangyo Kab. Hiyoshidai-ryo , KAWABATA, Hiroshi, Aburesuto Iwakura 1204 , KITA, Keiko, Godoshukusha Kosanjyutaku 3-105
IPC分类号： C12N9/02 , C12N15/53 , C12N15/63 , C12N1/21 , C12P7/62
CPC分类号： C12P7/62 , C12N9/0006 , C12P41/002
摘要： An enzyme having carbonyl reduction activity of reducing a carbonyl compound asymmetrically to produce an optically active alcohol, a DNA coding the enzyme, a plasmid having the DNA, a transformant which is a cell transformed with the plasmid, and a production method of an optically active alcohol using the enzyme and/or the transformed cell are provided.
摘要翻译：还原羰基化合物的具有酶活羰基还原活性不对称产生对光学活性醇，DNA编码的酶，具有DNA，转化体的所有其是用该质粒转化的细胞，和光学活性的制造方法的质粒同时提供了使用酶和/或转化的细胞醇。

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