专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20080044926A1 Reagent for Assaying Antigen and Method of Assaying Antigen 审中-公开
标题翻译：用于测定抗原的试剂和抗原的测定方法
公开(公告)号：US20080044926A1
公开(公告)日：2008-02-21
申请号：US11793980
申请日：2005-12-22
申请人： Yuki Honjo , Yuka Akimoto , Kazuo Kotani , Hirokazu Yago , Masanao Matsuo , Osamu Miyazaki , Kazunori Saito
发明人： Yuki Honjo , Yuka Akimoto , Kazuo Kotani , Hirokazu Yago , Masanao Matsuo , Osamu Miyazaki , Kazunori Saito
IPC分类号： G01N33/566
CPC分类号： G01N33/54333 , G01N33/54313 , G01N33/57434
摘要： It is intended to provide a reagent and an assay method whereby an antigen can be conveniently and accurately assayed without resorting to any special antibody. Using two or more carriers having different particle sizes, a carrier having a smaller particle size is sensitized with at least one monoclonal antibody, which is selected from among three monoclonal antibodies being reactive with both of a free antigen and a complex antigen and having different recognition sites from each other, while a carrier having a larger particle size is sensitized with the remainder monoclonal antibodies, thereby providing an assay reagent and an assay method whereby the reactivities of a free antigen and a complex antigen can be controlled.
摘要翻译：旨在提供一种试剂和测定方法，其中可以方便和准确地测定抗原而不诉诸任何特殊的抗体。使用两种或更多种具有不同粒度的载体，具有较小粒度的载体用至少一种单克隆抗体敏化，所述单克隆抗体选自与游离抗原和复合抗原两者具有不同识别性的三种单克隆抗体而具有较大粒径的载体则与剩余的单克隆抗体敏感，从而提供测定试剂和测定方法，由此可以控制游离抗原和复合抗原的反应性。

2. 发明申请

US20070065891A1 Method of pretreating sample and immunological assay method using the same 有权
标题翻译：预处理样品的方法和使用其的免疫测定方法
公开(公告)号：US20070065891A1
公开(公告)日：2007-03-22
申请号：US10575711
申请日：2004-10-15
申请人： Hiroyuki Ebinuma , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi
发明人： Hiroyuki Ebinuma , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi
IPC分类号： G01N33/569 , G01N33/554
CPC分类号： G01N33/74 , G01N2333/72 , Y10T436/25
摘要： The invention provides a method of pretreating a sample for conveniently, quickly and accurately measuring the total amount of adiponectin present in a biological sample contaminated with various adiponectin multimers. The method of measuring an sample for immunologically assaying the total amount of adiponectin present in the sample comprises reacting, with an adiponectin-containing sample, at least one of a reducing agent, an acid or a salt thereof, a surfactant, and a protease.
摘要翻译：本发明提供一种方法，用于方便，快速和准确地测量存在于受各种脂联素多聚体污染的生物样品中的脂连蛋白的总量的样品。用于免疫测定样品中存在的脂联素总量的样品的测定方法包括使含有脂联素的样品与还原剂，其酸或其盐，表面活性剂和蛋白酶中的至少一种反应。

3. 发明授权

US08039223B2 Method of selectively assaying adiponectin multimers 有权
标题翻译：选择性测定脂连蛋白多聚体的方法
公开(公告)号：US08039223B2
公开(公告)日：2011-10-18
申请号：US12468440
申请日：2009-05-19
申请人： Hiroyuki Ebinuma , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi , Kazuo Hara
发明人： Hiroyuki Ebinuma , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi , Kazuo Hara
IPC分类号： G01N33/53
CPC分类号： G01N33/74 , G01N2800/042 , G01N2800/32
摘要： Kits and methods for selectively assaying a target adiponectin multimer in a biological sample. Such methods accurately evaluate the relationship between a disease and adiponectin through selective assay of adiponectin multimers and provide information that cannot be obtained through measurement of the total amount of adiponectin alone. A method for selectively assaying a target adiponectin multimer in a biological sample comprising distinguishing target adiponectin multimer from the other adiponectin multimers by using a protease and/or an antibody.
摘要翻译：用于选择性测定生物样品中目标脂联素多聚体的试剂盒和方法。这种方法通过脂联素多聚体的选择性测定来准确地评估疾病与脂联素之间的关系，并提供通过单独测量脂连蛋白的总量不能获得的信息。一种用于选择性测定生物样品中的目标脂联素多聚体的方法，包括通过使用蛋白酶和/或抗体将目标脂连蛋白多聚体与其它脂联素多聚体区分开。

4. 发明申请

US20090291461A1 METHOD OF SELECTIVELY ASSAYING ADIPONECTIN MULTIMERS 有权
标题翻译：选择ADIPONECTIN多聚体的方法
公开(公告)号：US20090291461A1
公开(公告)日：2009-11-26
申请号：US12468440
申请日：2009-05-19
申请人： Hiroyuki EBINUMA , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi , Kazuo Hara
发明人： Hiroyuki EBINUMA , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi , Kazuo Hara
IPC分类号： G01N33/53
CPC分类号： G01N33/74 , G01N2800/042 , G01N2800/32
摘要： Kits and methods for selectively assaying a target adiponectin multimer in a biological sample. Such methods accurately evaluate the relationship between a disease and adiponectin through selective assay of adiponectin multimers and provide information that cannot be obtained through measurement of the total amount of adiponectin alone. A method for selectively assaying a target adiponectin multimer in a biological sample comprising distinguishing target adiponectin multimer from the other adiponectin multimers by using a protease and/or an antibody.
摘要翻译：用于选择性测定生物样品中目标脂联素多聚体的试剂盒和方法。这种方法通过脂联素多聚体的选择性测定来准确地评估疾病与脂联素之间的关系，并提供通过单独测量脂连蛋白的总量不能获得的信息。一种用于选择性测定生物样品中的目标脂联素多聚体的方法，包括通过使用蛋白酶和/或抗体将目标脂连蛋白多聚体与其它脂联素多聚体区分开。

5. 发明授权

US07608405B2 Method of selectively assaying adiponectin multimers 有权
标题翻译：选择性测定脂连蛋白多聚体的方法
公开(公告)号：US07608405B2
公开(公告)日：2009-10-27
申请号：US10575931
申请日：2004-10-15
申请人： Hiroyuki Ebinuma , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi , Kazuo Hara
发明人： Hiroyuki Ebinuma , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi , Kazuo Hara
IPC分类号： G01N33/53 , G01N33/543 , A61K39/395
CPC分类号： G01N33/74 , G01N2800/042 , G01N2800/32
摘要： Methods for selectively assaying a target adiponectin multimer in a biological sample. Such methods accurately evaluate the relationship between a disease and adiponectin through selective assay of adiponectin multimers and provide information that cannot be obtained through measurement of the total amount of adiponectin alone. A method for selectively assaying of a target adiponectin multimer in a biological sample comprising distinguishing target adiponectin multimer from the other adiponectin multimers by using a protease and/or an antibody.
摘要翻译：用于选择性测定生物样品中目标脂联素多聚体的方法。这种方法通过脂联素多聚体的选择性测定来准确地评估疾病与脂联素之间的关系，并提供通过单独测量脂连蛋白的总量不能获得的信息。一种用于选择性测定生物样品中的目标脂联素多聚体的方法，包括通过使用蛋白酶和/或抗体将目标脂连蛋白多聚体与其他脂联素多聚体区分开。

6. 发明申请

US20070042424A1 Method of selectively assaying adiponectin multimers 有权
标题翻译：选择性测定脂连蛋白多聚体的方法
公开(公告)号：US20070042424A1
公开(公告)日：2007-02-22
申请号：US10575931
申请日：2004-10-15
申请人： Hiroyuki Ebinuma , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi , Kazuo Hara
发明人： Hiroyuki Ebinuma , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi , Kazuo Hara
IPC分类号： G01N33/53 , C12Q1/37
CPC分类号： G01N33/74 , G01N2800/042 , G01N2800/32
摘要： We have discovered a “human adiponectin ELISA kit”, which can specifically measure adiponectin in human blood serum (or blood plasma) or in extracted fluid from lipocytes or culture supernatant fluid. After fractioning human serum by use of gel filtration column, each fraction was measured by the present kit, and as a result, adiponectin immunity activity was detected as a plurality of peaks at above 670 kD of molecular weight. From the results, it is presumed that adiponectin is present in the blood as a polymer or forms a complex with polymer proteins.
摘要翻译：我们已经发现了一种“人类脂联素ELISA试剂盒”，可以特异性地测量人血清（或血浆）中的脂联素或来自脂肪细胞或培养上清液的提取液。通过使用凝胶过滤柱对人血清进行分馏后，通过本试剂盒测定各级分，结果，在670kD以上的分子量下检测出多个峰的脂联素免疫活性。从结果可以推测，脂联素作为聚合物存在于血液中，或与聚合物蛋白形成复合物。

7. 发明授权

US07781171B2 Mehod of pretreating sample and immunological assay method using the same 有权
标题翻译：预处理样品的方法和使用其的免疫测定方法
公开(公告)号：US07781171B2
公开(公告)日：2010-08-24
申请号：US10575711
申请日：2004-10-15
申请人： Hiroyuki Ebinuma , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi
发明人： Hiroyuki Ebinuma , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi
IPC分类号： G01N33/53
CPC分类号： G01N33/74 , G01N2333/72 , Y10T436/25
摘要： The invention provides a method of pretreating a sample for conveniently, quickly and accurately measuring the total amount of adiponectin present in a biological sample contaminated with various adiponectin multimers.The method of measuring an sample for immunologically assaying the total amount of adiponectin present in the sample comprises reacting, with an adiponectin-containing sample, at least one of a reducing agent, an acid or a salt thereof, a surfactant, and a protease.
摘要翻译：本发明提供一种方法，用于方便，快速和准确地测量存在于受各种脂联素多聚体污染的生物样品中的脂连蛋白的总量的样品。用于免疫测定样品中存在的脂联素总量的样品的测定方法包括使含有脂联素的样品与还原剂，其酸或其盐，表面活性剂和蛋白酶中的至少一种反应。

8. 发明授权

US5843677A Method of assaying asialoglycoprotein receptor and assay reagent therefor 失效
标题翻译：测定脱唾液酸糖蛋白受体的方法及其试剂
公开(公告)号：US5843677A
公开(公告)日：1998-12-01
申请号：US722153
申请日：1996-10-18
申请人： Yoshiro Niitsu , Yutaka Kohgo , Junji Kato , Hirokazu Yago , Hisashi Hanada , Koji Ushizawa
发明人： Yoshiro Niitsu , Yutaka Kohgo , Junji Kato , Hirokazu Yago , Hisashi Hanada , Koji Ushizawa
IPC分类号： G01N33/53 , G01N33/531 , G01N33/543 , G01N33/577 , G01N33/68 , C07K16/28
CPC分类号： G01N33/68
摘要： The present invention is directed to a method of immunoassay of asialoglycoprotein receptors (AGPR) by bringing a specimen into contact with a monoclonal antibody that recognizes AGPR, wherein the pH of a diluted specimen solution is adjusted from 5 to 7 or phenol is added to a solution of an enzyme-labeled antibody. This method is excellent in the sensitivity and accuracy of AGPR assay and can be applied also to the screening of hepatopathy remedies.
摘要翻译： PCT No.PCT / JP95 / 00770 Sec。 371日期：1996年10月18日 102（e）日期1996年10月18日PCT提交1995年4月19日PCT公布。 WO95 / 29403 PCT出版物日期：1995年11月2日本发明涉及一种通过使样品与识别AGPR的单克隆抗体接触的免疫测定来测定脱唾液酸糖蛋白受体（AGPR）的方法，其中将稀释样品溶液的pH调节为5〜7或将苯酚加入到酶标记抗体的溶液中。该方法在AGPR测定的灵敏度和准确性方面优异，也可用于筛查肝病治疗。

9. 发明授权

US08580532B2 Method for stabilizing α-thrombin in thrombin-containing solution 有权
标题翻译：在凝血酶溶液中稳定α-凝血酶的方法
公开(公告)号：US08580532B2
公开(公告)日：2013-11-12
申请号：US12519191
申请日：2007-12-06
申请人： Remi Ikeda , Chizuru Morikawa , Hirokazu Yago
发明人： Remi Ikeda , Chizuru Morikawa , Hirokazu Yago
IPC分类号： A61K38/43 , A61K9/19
CPC分类号： G01N33/86
摘要： To provide a method for stabilizing unstable α-thrombin in a thrombin-containing solution, a solution containing stabilized α-thrombin, and a liquid fibrinogen assay reagent containing the solution. The method for stabilizing α-thrombin in a thrombin-containing solution, which includes adjusting the percentage of α-thrombin to 70% or more with respect to the amount of total thrombin in the thrombin-containing solution.
摘要翻译：提供一种用于在含凝血酶的溶液中稳定不稳定的α-凝血酶的方法，含有稳定的α-凝血酶的溶液和含有该溶液的液体纤维蛋白原测定试剂。用于在凝血酶溶液中稳定α-凝血酶的方法，其包括相对于含凝血酶的溶液中的总凝血酶的量调节α-凝血酶的百分比为70％以上。

10. 发明申请

US20110129862A1 METHOD FOR DETERMINING CAUSE OF THE PROLONGATION OF BLOOD COAGULATION TIME 审中-公开
标题翻译：用于确定血液凝聚时间延长的原因的方法
公开(公告)号：US20110129862A1
公开(公告)日：2011-06-02
申请号：US12999527
申请日：2009-06-16
申请人： Remi Nakamura , Chizuru Morikawa , Hiroyuki Sunaga , Hirokazu Yago
发明人： Remi Nakamura , Chizuru Morikawa , Hiroyuki Sunaga , Hirokazu Yago
IPC分类号： C12Q1/56 , G01N33/86
CPC分类号： G01N33/86
摘要： Provided is a method of accurately and easily determining a cause of the prolongation of blood coagulation time.The method of determining a cause of the prolongation of blood coagulation time in test plasma includes (1) measuring the blood coagulation time of samples including (a) the test plasma only, (b) normal plasma only, and (c) the test plasma and the normal plasma mixed at least at a mixing ratio, (2) drawing a polygonal line graph by plotting the measurement results of the samples (a), (b) and (c), with the vertical axis representing the blood coagulation time or the prolongation ratio of blood coagulation time and the horizontal axis representing the mixing ratio or mixing proportion of the test plasma or the normal plasma, and thereby determining the area A under the polygonal line and the area B under a line segment that connects the plotted measurement results of the samples (a) and (b), (3) calculating the ratio of the area (A−B) obtained by subtracting the area B from the area A, with respect to the area B, ((A−B)/(B)):area ratio X), (4) performing the steps of (1) and (2) for a coagulation factor inhibitor-positive plasma, and thereby determining in advance a standard area ratio Y, and (5) comparing the area ratio X and the standard area ratio Y, and determining the test plasma as a coagulation factor inhibitor type in the case where Y≦X, or as a coagulation factor deficient type in the case where Y>X.
摘要翻译：提供了准确且容易地确定凝血时间延长的原因的方法。测定血浆凝血时间延长的原因的方法包括：（1）测定样品的凝血时间，包括（a）仅测试血浆，（b）只有正常血浆，（c）测试血浆和至少以混合比混合的正常等离子体，（2）通过绘制样品（a），（b）和（c）的测量结果绘制折线图，垂直轴代表凝血时间，或血液凝固时间的延长率和横轴表示试验等离子体或正常等离子体的混合比例或混合比例，由此确定在折线下面积A和连接图形测量值的线段下的面积B 样品（a）和（b）的结果，（3）计算通过从面积A减去面积B而获得的面积（A-B）相对于面积B的比例（（A-B） /（B））：面积比X），（4）进行（1）和（2）的步骤凝血因子抑制剂阳性血浆，由此预先确定标准面积比Y，和（5）比较面积比X和标准面积比Y，并将测试血浆确定为凝血因子抑制剂类型， Y，X，或作为Y> X的情况下的凝血因子缺陷型。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式