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1. 发明申请

US20050175997A1 Method of uniformizing dna fragment contents and subtraction method 审中-公开
标题翻译： dna片段内容均匀化和减法方法
公开(公告)号：US20050175997A1
公开(公告)日：2005-08-11
申请号：US10481228
申请日：2002-06-19
申请人： Yuichi Ono , Toshio Imai
发明人： Yuichi Ono , Toshio Imai
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q2531/113 , C12Q2525/191 , C12Q2525/131
摘要： A method of normalizing contents of DNA fragments in a sample among respective DNA fragments, comprising: preparing DNA fragments in which adaptors each comprising an oligodeoxyribonucleotide are attached to both ends of each DNA fragment in the sample to form restriction enzyme recognition sites that do not exist in the DNA fragments in the sample, and at least a part between the restriction enzyme recognition sites at both ends, including the restriction enzyme recognition sites, is double-stranded; denaturing the prepared double-stranded DNA fragments; hybridizing the denatured DNA fragments under a condition that a part of the DNA fragments remains single-stranded; cleaving the hybridized double-stranded DNA fragments with a restriction enzyme having a cleavage site exclusively in the adaptors; and performing PCR using the obtained DNA fragments as templates and using a primer having a nucleotide sequence complementary to a nucleotide sequence of the adaptors before the cleavage; and a subtraction method comprising performing normalization by the method.
摘要翻译：使各DNA片段中的样品中DNA片段的含量正常化的方法，包括：制备DNA片段，其中每个包含寡脱氧核糖核苷酸的衔接子连接到样品中每个DNA片段的两端，以形成不存在的限制酶识别位点在样品中的DNA片段中，两端的限制酶识别位点（包括限制酶识别位点）之间的至少一部分是双链的; 使制备的双链DNA片段变性; 在一部分DNA片段保持单链的条件下使变性DNA片段杂交; 用具有切割位点的限制酶切割杂交的双链DNA片段，仅在适配器中; 并使用所获得的DNA片段作为模板并使用具有与切割前的衔接子的核苷酸序列互补的核苷酸序列的引物进行PCR; 以及减法方法，包括通过该方法执行归一化。

2. 发明授权

US07824676B2 Exocrine gland tight junction-constituting protein jeap family 失效
标题翻译：外分泌腺紧密连接蛋白家族
公开(公告)号：US07824676B2
公开(公告)日：2010-11-02
申请号：US11522043
申请日：2006-09-15
申请人： Miyuki Nishimura , Mayumi Asano , Yuichi Ono , Koji Morimoto , Masakazu Takeuchi , Yoko Inoue , Toshio Imai , Yoshimi Takai
发明人： Miyuki Nishimura , Mayumi Asano , Yuichi Ono , Koji Morimoto , Masakazu Takeuchi , Yoko Inoue , Toshio Imai , Yoshimi Takai
IPC分类号： C07K16/18
CPC分类号： C07K14/47 , C07K14/705
摘要： A mouse cDNA library from gene fragments encoding proteins localizing at cell-cell junctions was screened by a technique visualizing localization of a protein to identify a junction-enriched and -associated protein, JEAP. GenBank homology search was performed based on the sequence. Based on the obtained sequence, a mouse cDNA library was screened to identify JEAP-2. By using prepared antibodies against these proteins, it was revealed that these proteins express specifically at tight junctions, in particular, tight junctions in exocrine glands.
摘要翻译：通过技术可视化蛋白质的定位技术筛选编码在细胞连接处定位的蛋白质的蛋白质的基因片段的小鼠cDNA文库，以鉴定连接蛋白和相关蛋白JEAP。 GenBank同源性检索是根据序列进行的。基于获得的序列，筛选小鼠cDNA文库以鉴定JEAP-2。通过使用针对这些蛋白质的制备的抗体，显示这些蛋白质在紧密连接处特别是在外分泌腺中的紧密连接处特异性表达。

3. 发明申请

US20080199437A1 Lrp4/Corin Dopaminergic Neuron Progenitor Cell Markers 审中-公开
标题翻译： Lrp4 / Corin多巴胺能神经元祖细胞标记物
公开(公告)号：US20080199437A1
公开(公告)日：2008-08-21
申请号：US10552485
申请日：2005-07-22
申请人： Yoshimasa Sakamoto , Yuichi Ono , Toshio Imai , Yasuko Nakagawa
发明人： Yoshimasa Sakamoto , Yuichi Ono , Toshio Imai , Yasuko Nakagawa
IPC分类号： A61K35/12 , C07H21/04 , C12N5/06 , C12Q1/68 , A61P25/16 , C07K17/02 , C12Q1/02 , C07K16/18
CPC分类号： C12N5/0623 , A61K35/30 , C07K16/286 , C12Q1/6881 , C12Q1/6883 , C12Q2600/158 , G01N33/5023 , G01N33/56966 , G01N33/6896 , G01N2500/00 , G01N2800/2835
摘要： The present invention relates to polynucleotide probes and antibodies for detecting Lrp4/Corin dopaminergic neuron progenitor cell markers, which enable the efficient separation of dopaminergic neuron progenitor cells; and methods for selecting the progenitor cells by the use thereof.
摘要翻译：本发明涉及用于检测Lrp4 / Corin多巴胺能神经元祖细胞标记物的多核苷酸探针和抗体，其能够有效分离多巴胺能神经元祖细胞; 以及通过其使用来选择祖细胞的方法。

4. 发明申请

US20080119641A1 Exocrine gland tight junction-constituting protein JEAP family 失效
标题翻译：外分泌腺紧密连接蛋白JEAP家族
公开(公告)号：US20080119641A1
公开(公告)日：2008-05-22
申请号：US11522043
申请日：2006-09-15
申请人： Miyuki Nishimura , Mayumi Asano , Yuichi Ono , Koji Morimoto , Masakazu Takeuchi , Yoko Inoue , Toshio Imai , Yoshimi Takai
发明人： Miyuki Nishimura , Mayumi Asano , Yuichi Ono , Koji Morimoto , Masakazu Takeuchi , Yoko Inoue , Toshio Imai , Yoshimi Takai
IPC分类号： C07K16/18
CPC分类号： C07K14/47 , C07K14/705
摘要： A mouse cDNA library from gene fragments encoding proteins localizing at cell—cell junctions was screened by a technique visualizing localization of a protein to identify a junction-enriched and -associated protein, JEAP. GenBank homology search was performed based on the sequence. Based on the obtained sequence, a mouse cDNA library was screened to identify JEAP-2. By using prepared antibodies against these proteins, it was revealed that these proteins express specifically at tight junctions, in particular, tight junctions in exocrine glands.
摘要翻译：通过技术可视化蛋白质的定位技术筛选编码在细胞连接处定位的蛋白质的蛋白质的基因片段的小鼠cDNA文库，以鉴定连接蛋白和相关蛋白JEAP。 GenBank同源性检索是根据序列进行的。基于获得的序列，筛选小鼠cDNA文库以鉴定JEAP-2。通过使用针对这些蛋白质的制备的抗体，显示这些蛋白质在紧密连接处特别是在外分泌腺中的紧密连接处特异性表达。

5. 发明授权

US07129063B2 Exocrine gland tight junction-constituting protein jeap family 失效
公开(公告)号：US07129063B2
公开(公告)日：2006-10-31
申请号：US10298417
申请日：2002-11-15
申请人： Miyuki Nishimura , Mayumi Asano , Yuichi Ono , Koji Morimoto , Masakazu Takeuchi , Yoko Inoue , Toshio Imai , Yoshimi Takai
发明人： Miyuki Nishimura , Mayumi Asano , Yuichi Ono , Koji Morimoto , Masakazu Takeuchi , Yoko Inoue , Toshio Imai , Yoshimi Takai
IPC分类号： C12P21/02 , C12N15/63 , C12N1/21 , C12N1/19 , C12N5/10 , C07H21/04
CPC分类号： C07K14/47 , C07K14/705
摘要： A mouse cDNA library from gene fragments encoding proteins localizing at cell-cell junctions was screened by a technique visualizing localization of a protein to to identify a junction-enriched and -associated protein, JEAP. GenBank homology search was performed based on the sequence. Based on the obtained sequence, a mouse cDNA library was screened to identify JEAP-2. By using prepared antibodies against these proteins, it was revealed that these proteins express specifically at tight junctions, in particular, tight junctions in exocrine glands.

6. 发明申请

US20140193836A1 NOVEL MARKERS FOR DOPAMINERGIC NEURON PROGENITOR CELLS 有权
标题翻译： DOPAMINERGIC神经元祖细胞的新标志
公开(公告)号：US20140193836A1
公开(公告)日：2014-07-10
申请号：US14234361
申请日：2012-07-27
申请人： Jun Takahashi , Daisuke Doi , Bumpei Samata , Yuichi Ono
发明人： Jun Takahashi , Daisuke Doi , Bumpei Samata , Yuichi Ono
IPC分类号： G01N33/569
CPC分类号： G01N33/56966 , C12N5/0619 , C12N5/0623 , C12N2501/155 , C12N2501/16 , C12N2501/727 , C12N2502/1394 , C12N2506/02 , C12N2506/45 , C12N2509/00
摘要： The present invention provides a method for selecting dopaminergic neuron progenitor cells, which comprises detecting any one or more of markers selected from the group consisting of CD15 (SSEA-1), CD24, CD46, CD47, CD49b, CD57, CD58, CD59, CD81, CD90, CD98, CD147, CD184, Disalogangliosid GD2, SSEA-4, CD49f, SERINC4, CCR9, PHEX, TMPRSS11E, HTR1E, SLC25A2, Ctxn3, Cc17, Chrnb4, Chrna3, Kcnv2, Grm2, Syt2, Lim2, Mboat1, St3ga16, Slc39a12, Tacr1, Lrtm1, Dscam and CD201.
摘要翻译：本发明提供了选择多巴胺能神经元祖细胞的方法，其包括检测选自CD15（SSEA-1），CD24，CD46，CD47，CD49b，CD57，CD58，CD59，CD81的任何一种或多种标志物，CD90，CD98，CD147，CD184，Disalogangliosid GD2，SSEA-4，CD49f，SERINC4，CCR9，PHEX，TMPRSS11E，HTR1E，SLC25A2，Ctxn3，Cc17，Chrnb4，Chrna3，Kcnv2，Grm2，Syt2，Lim2，Mboat1，St3ga16， Slc39a12，Tacr1，Lrtm1，Dscam和CD201。

7. 发明申请

US20120252021A1 DOPAMINERGIC NEURON PROGENITOR CELL MARKER 187A5 有权
标题翻译：多巴胺神经元细胞标记187A5
公开(公告)号：US20120252021A1
公开(公告)日：2012-10-04
申请号：US13492733
申请日：2012-06-08
申请人： Yuichi Ono , Yoshimasa Sakamoto
发明人： Yuichi Ono , Yoshimasa Sakamoto
IPC分类号： C12Q1/68 , C07H21/00
CPC分类号： C07K14/705 , A61K35/30 , A61K38/00 , C07K16/28 , C12Q1/6881 , C12Q2600/158 , G01N33/5058 , G01N33/5073 , G01N33/56966 , G01N2333/70571 , Y10S530/81
摘要： An object of the present invention is to provide a probe, a primer, a primer set and an antibody for use in the detection or selection of a dopaminergic neuron progenitor cell. The present invention provides a probe, a primer and a primer set for use in the detection or selection of a mesencephalon dopaminergic neuron progenitor cell, and preferably a dopaminergic neuron proliferative progenitor cell, which can hybridize with a nucleotide sequence of a 187A5 gene, or a complementary sequence thereto, and an antibody for use in the detection or selection of a mesencephalon dopaminergic neuron progenitor cell, and preferably a dopaminergic neuron progenitor cell, which is capable of binding to a 187A5 protein.
摘要翻译：本发明的目的是提供用于检测或选择多巴胺能神经元祖细胞的探针，引物，引物组和抗体。本发明提供了用于检测或选择中脑多巴胺能神经元祖细胞的探针，引物和引物组，优选多巴胺能神经元增殖祖细胞，其可以与187A5基因的核苷酸序列杂交，或其互补序列，以及用于检测或选择能够结合187A5蛋白的中脑多巴胺能神经元祖细胞，优选多巴胺能神经元祖细胞的抗体。

8. 发明申请

US20120178083A1 SPECIFIC MARKER Lmx1a ON DOPAMINERGIC NEURONS 有权
标题翻译：特异性标记Lmx1a在多巴胺神经元上
公开(公告)号：US20120178083A1
公开(公告)日：2012-07-12
申请号：US13352241
申请日：2012-01-17
申请人： Yuichi Ono , Yasuko Nakagawa , Tomoya Nakatani
发明人： Yuichi Ono , Yasuko Nakagawa , Tomoya Nakatani
IPC分类号： C12Q1/68 , C07K16/18 , G01N33/566
CPC分类号： C12Q1/6881 , C12Q1/6883 , C12Q2600/158 , G01N33/6896 , G01N33/9413 , G01N2800/2835
摘要： The present invention identified Lmx1a genes, which are expressed in dopaminergic neurons at all differentiation stages, from proliferating dopaminergic neuron progenitor cells before cell cycle exit to cells after cell cycle exit. Lmx1a expression in cells can be used as an indicator when selecting cells suitable for transplantation therapy for neurodegenerative diseases such as Parkinson's disease, and is useful as a marker for screening agents involved in the induction of dopaminergic neuron differentiation.
摘要翻译：本发明鉴定了在细胞周期退出至细胞周期后出现的细胞周期性增殖的多巴胺能神经元祖细胞中，在所有分化阶段的多巴胺能神经元中表达的Lmx1a基因。当选择适合于帕金森病等神经变性疾病的移植治疗的细胞时，细胞中的Lmx1a表达可用作指示剂，并且可用作参与多巴胺能神经元分化诱导的筛选剂的标记。

9. 发明申请

US20090211916A1 Method and apparatus for producing metal by electrolysis of molton salt 审中-公开
标题翻译：通过电解钼矿盐生产金属的方法和设备
公开(公告)号：US20090211916A1
公开(公告)日：2009-08-27
申请号：US11631364
申请日：2005-06-27
申请人： Masanori Yamaguchi , Yuichi Ono , Susumu Kosemura , Eiji Nishimura
发明人： Masanori Yamaguchi , Yuichi Ono , Susumu Kosemura , Eiji Nishimura
IPC分类号： C25C3/00 , C25C7/00
CPC分类号： C25C7/007 , C25C3/00 , C25C7/08
摘要： A process for production of a metal includes a step of filling a metal chloride in an electrolysis vessel having positive and negative electrodes, a step of heating and fusing the metal chloride to make an electrolytic bath, and a step of electrolyzing the electrolytic bath to deposit metal on the negative electrode in a solid state. In addition, in an apparatus for production of a metal in which a metal chloride is filled in an electrolysis vessel having positive and negative electrodes, the metal chloride is heated and molten to make an electrolytic bath and the electrolytic bath is electrolyzed to deposit the metal on the negative electrode in a solid state, the electrolytic bath is divided into an electrolysis chamber and a dissolution chamber by a dividing wall, the positive electrode is arranged in the electrolysis chamber, the negative electrode is arranged to enable orbital movement in a circle through the electrolysis chamber and dissolution chamber, and the metal deposited on the negative electrode in the electrolysis chamber is separated and recovered in the dissolution chamber.
摘要翻译：金属的制造方法包括在具有正极和负极的电解容器中填充金属氯化物的步骤，对金属氯化物进行加热和熔融以形成电解槽的步骤，以及电解槽沉积步骤金属在负极上固态。另外，在具有正极和负极的电解容器中填充有金属氯化物的金属的制造装置中，对金属氯化物进行加热熔融，形成电解槽，电解槽被电解以沉积金属在固态的负极上，通过分隔壁将电解槽分为电解室和溶解室，正极配置在电解室中，负极配置成能够以圆周方式轨道运动电解室和溶解室，并且沉积在电解室中的负极上的金属在溶解室中分离回收。

10. 发明申请

US20080280301A1 Lrp4/Corin DOPAMINERGIC NEURON PROLIFERATIVE PROGENITOR CELL MARKERS 有权
标题翻译： Lrp4 / Corin DOPAMINERGIC神经元增殖型细胞标志物
公开(公告)号：US20080280301A1
公开(公告)日：2008-11-13
申请号：US12110111
申请日：2008-04-25
申请人： Yuichi Ono , Yasuko Nakagawa , Yoshimasa Sakamoto
发明人： Yuichi Ono , Yasuko Nakagawa , Yoshimasa Sakamoto
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q2600/158
摘要： In neuron transplantation therapy, in terms of safety, it is preferable to use a cell population consisting only of a desired type of cells, and to use postmitotic neurons in consideration to avoid the risk of tumorigenesis. Moreover, greater therapeutic effects would be expected through the use of earlier progenitor cells in consideration of post-transplantation viability, proper network formation ability, and such.According to the present invention, Lrp4, a gene that is specifically expressed in dopaminergic neuron proliferative progenitor cells prior to cell cycle exit, was identified. The use of Lrp4 expression in cells as an index allows for the isolation. of cells suitable for transplantation therapy of neurodegenerative diseases such as Parkinson's disease in terms of safety, survival rate, and network formation ability.
摘要翻译：在神经元移植治疗中，在安全性方面，优选使用仅由期望类型的细胞组成的细胞群，并考虑使用神经元神经元以避免肿瘤发生的风险。此外，考虑到移植后的生存力，适当的网络形成能力等，通过使用较早的祖细胞可以预期更大的治疗效果。根据本发明，鉴定了在细胞周期退出之前在多巴胺能神经元增殖祖细胞中特异性表达的基因Lrp4。在细胞中使用Lrp4表达作为指标，可以进行分离。的细胞在安全性，存活率和网络形成能力方面适用于帕金森病等神经变性疾病的移植治疗。

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