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1. 发明申请

US20050037414A1 PCR primer set for detecting hepatitis B virus, method for detecting hepatitis B using the primer set, and hepatitis B virus detection kit including the primer set 有权
标题翻译：用于检测乙型肝炎病毒的PCR引物组，使用引物组检测乙型肝炎的方法，以及包括引物组的乙型肝炎病毒检测试剂盒
公开(公告)号：US20050037414A1
公开(公告)日：2005-02-17
申请号：US10918000
申请日：2004-08-13
申请人： Young-sun Lee , Jung-im Han , Jung-joo Hwang , Mi-kyung Kim , Yoon-kyoung Cho , Hee-kyun Lim , Young-a Kim
发明人： Young-sun Lee , Jung-im Han , Jung-joo Hwang , Mi-kyung Kim , Yoon-kyoung Cho , Hee-kyun Lim , Young-a Kim
IPC分类号： C12N15/09 , C12Q1/68 , C12Q1/70 , C12P19/34
CPC分类号： C12Q1/706 , C12Q2565/629 , C12Q2565/543 , C12Q2531/113
摘要： Provided are a primer set selected from the group consisting of primers having nucleotide sequences as set forth in SEQ ID NOS: 1-40, a method for detecting hepatitis B virus by polymerase chain reaction (PCR) using the primer set, and a hepatitis B virus detection kit including the primer set.
摘要翻译：提供了选自由SEQ ID NO：1-40所示的核苷酸序列的引物组成的引物组，使用引物组的聚合酶链反应（PCR）检测乙型肝炎病毒的方法，以及乙型肝炎病毒检测试剂盒包括引物组。

2. 发明授权

US07344837B2 PCR primer set for detecting hepatitis B virus, method for detecting hepatitis B using the primer set, and hepatitis B virus detection kit including the primer set 有权
标题翻译：用于检测乙型肝炎病毒的PCR引物组，使用引物组检测乙型肝炎的方法，以及包括引物组的乙型肝炎病毒检测试剂盒
公开(公告)号：US07344837B2
公开(公告)日：2008-03-18
申请号：US10918000
申请日：2004-08-13
申请人： Young-sun Lee , Jung-im Han , Jung-joo Hwang , Mi-kyung Kim , Yoon-kyoung Cho , Hee-kyun Lim , Young-a Kim
发明人： Young-sun Lee , Jung-im Han , Jung-joo Hwang , Mi-kyung Kim , Yoon-kyoung Cho , Hee-kyun Lim , Young-a Kim
IPC分类号： C12Q1/70
CPC分类号： C12Q1/706 , C12Q2565/629 , C12Q2565/543 , C12Q2531/113
摘要： Provided are a primer set selected from the group consisting of primers having nucleotide sequences as set forth in SEQ ID NOS: 1–40, a method for detecting hepatitis B virus by polymerase chain reaction (PCR) using the primer set, and a hepatitis B virus detection kit including the primer set.
摘要翻译：提供了选自由SEQ ID NO：1-40所示的核苷酸序列的引物组成的引物组，使用引物组的聚合酶链反应（PCR）检测乙型肝炎病毒的方法，以及乙型肝炎病毒检测试剂盒包括引物组。

3. 发明授权

US07722751B2 Apparatus for regulating salt concentration using electrodialysis, lab-on-a-chip including the same, and method of regulating salt concentration using the apparatus 有权
标题翻译：使用电渗析法调节盐浓度的装置，包括该盐浓度的实验室的方法以及使用该装置调节盐浓度的方法
公开(公告)号：US07722751B2
公开(公告)日：2010-05-25
申请号：US11440872
申请日：2006-05-25
申请人： Jung-im Han , Young-sun Lee , Joon-ho Kim
发明人： Jung-im Han , Young-sun Lee , Joon-ho Kim
IPC分类号： B01D61/48
CPC分类号： B01D61/422 , B01D61/46 , B01L3/5027 , G01N2001/4011
摘要： An apparatus for regulating salt concentration, a lab-on-a-chip including the same and a method of regulating salt concentration using the apparatus are provided. The apparatus includes: a reaction chamber that is defined by a cation exchange membrane and an anion exchange membrane and is selected from the group consisting of a biomolecule extraction chamber, an amplification chamber, a hybridization chamber, and a detection chamber; a first electrode chamber that is defined by the anion exchange membrane and a first electrode and includes an ion exchange medium; and a second electrode chamber that is defined by the cation exchange membrane and a second electrode and includes an ion exchange medium. Even without injecting solutions with different salt concentrations into the reaction chamber by operating pumps and valves for each operation stage, the salt concentration can be reversibly regulated in situ by adjusting the polarity, intensity and application time of a voltage.
摘要翻译：提供了一种调节盐浓度的装置，包括该盐浓度的实验室，以及使用该装置调节盐浓度的方法。该装置包括：由阳离子交换膜和阴离子交换膜限定的反应室，其选自生物分子提取室，扩增室，杂交室和检测室; 由阴离子交换膜和第一电极限定并包括离子交换介质的第一电极室; 以及由阳离子交换膜和第二电极限定并包括离子交换介质的第二电极室。即使在每个操作阶段通过操作泵和阀都不会将具有不同盐浓度的溶液注入反应室中，可以通过调节电压的极性，强度和施加时间来原位调节盐浓度。

4. 发明授权

US07579151B2 Isolation and purification method of biomolecules using hydrogel 有权
标题翻译：使用水凝胶分离和纯化生物分子的方法
公开(公告)号：US07579151B2
公开(公告)日：2009-08-25
申请号：US11191221
申请日：2005-07-27
申请人： Young-sun Lee , Jung-im Han , Hyo-yeon Lee , Kak Namkoong , Kwang-wook Oh
发明人： Young-sun Lee , Jung-im Han , Hyo-yeon Lee , Kak Namkoong , Kwang-wook Oh
IPC分类号： C12Q1/68 , C07H21/00
CPC分类号： C12N15/1006
摘要： Provided is a method of isolating and purifying biomolecules using a hydrogel, the method including: bring a sample containing charged biomolecules into contact with a hydrogel to bind the biomolecules to the hydrogel; washing the hydrogel bound with the biomolecules; and eluting the bound biomolecules using an elution solvent. According to the method, the use of a hydrogel with a large surface area reduces the isolation time of biomolecules to 5 min or less, an external device such as an electromagnet is not required, and small-sized systems or LOC can be easily implemented due to applicability to microsystems through a polymer patterning technique.
摘要翻译：提供了使用水凝胶分离和纯化生物分子的方法，该方法包括：使含有带电生物分子的样品与水凝胶接触以将生物分子结合至水凝胶; 洗涤与生物分子结合的水凝胶; 并使用洗脱溶剂洗脱结合的生物分子。根据该方法，使用具有大表面积的水凝胶将生物分子的隔离时间减少到5分钟以下，不需要诸如电磁体的外部装置，并且可以容易地实现小尺寸系统或LOC 通过聚合物图案化技术适用于微系统。

5. 发明申请

US20060169339A1 Microvalve device and apparatus adopting the same 审中-公开
标题翻译：微阀装置及其采用的装置
公开(公告)号：US20060169339A1
公开(公告)日：2006-08-03
申请号：US11346452
申请日：2006-02-02
申请人： Kwang-wook Oh , Young-sun Lee , Jung-im Han
发明人： Kwang-wook Oh , Young-sun Lee , Jung-im Han
IPC分类号： F15C1/04
CPC分类号： F16K99/0003 , B01L3/502738 , B01L2300/0861 , B01L2400/0622 , B01L2400/0672 , F16K99/0001 , F16K99/0034 , F16K99/0042 , F16K2099/0069 , F16K2099/0074 , F16K2099/0084 , Y10T137/2191
摘要： A microvalve device is provided. The microvalve device uses electrolysis and uses a hydrogel swelling and deswelling in response to anions or cations as an actuator for controlling the path of a flowing fluid. The microvalve device does not require a buffer solution but uses the transfer fluid flowing in a valve as a source driving the actuator. To generate the anions or cations, an electrode is needed for electrolysis of the fluid near the hydrogel. The microvalve is easy to manufacture and has a simple structure. In addition, the micro valve is useful to manufacture fluid channel arrays having various multi-channel structures.
摘要翻译：提供微型阀装置。微阀装置使用电解，并且响应于阴离子或阳离子作为用于控制流动流体的路径的致动器，使用水凝胶溶胀和溶解。微阀装置不需要缓冲溶液，而是使用流过阀的转移流体作为驱动致动器的源。为了产生阴离子或阳离子，需要电极来电解水凝胶附近的流体。微型阀门易于制造，结构简单。此外，微阀可用于制造具有各种多通道结构的流体通道阵列。

6. 发明申请

US20060266650A1 Apparatus for regulating salt concentration using electrodialysis, lab-on-a-chip including the same, and method of regulating salt concentration using the apparatus 有权
标题翻译：使用电渗析法调节盐浓度的装置，包括该盐浓度的实验室的方法以及使用该装置调节盐浓度的方法
公开(公告)号：US20060266650A1
公开(公告)日：2006-11-30
申请号：US11440872
申请日：2006-05-25
申请人： Jung-im Han , Young-sun Lee , Joon-ho Kim
发明人： Jung-im Han , Young-sun Lee , Joon-ho Kim
IPC分类号： B01D61/42
CPC分类号： B01D61/422 , B01D61/46 , B01L3/5027 , G01N2001/4011
摘要： An apparatus for regulating salt concentration, a lab-on-a-chip including the same and a method of regulating salt concentration using the apparatus are provided. The apparatus includes: a reaction chamber that is defined by a cation exchange membrane and an anion exchange membrane and is selected from the group consisting of a biomolecule extraction chamber, an amplification chamber, a hybridization chamber, and a detection chamber; a first electrode chamber that is defined by the anion exchange membrane and a first electrode and includes an ion exchange medium; and a second electrode chamber that is defined by the cation exchange membrane and a second electrode and includes an ion exchange medium. Even without injecting solutions with different salt concentrations into the reaction chamber by operating pumps and valves for each operation stage, the salt concentration can be reversibly regulated in situ by adjusting the polarity, intensity and application time of a voltage.
摘要翻译：提供了一种调节盐浓度的装置，包括该盐浓度的实验室，以及使用该装置调节盐浓度的方法。该装置包括：由阳离子交换膜和阴离子交换膜限定的反应室，其选自生物分子提取室，扩增室，杂交室和检测室; 由阴离子交换膜和第一电极限定并包括离子交换介质的第一电极室; 以及由阳离子交换膜和第二电极限定并包括离子交换介质的第二电极室。即使在每个操作阶段通过操作泵和阀都不会将具有不同盐浓度的溶液注入反应室中，可以通过调节电压的极性，强度和施加时间来原位调节盐浓度。

7. 发明申请

US20060127887A1 Isolation and purification method of biomolecules using hydrogel 有权
公开(公告)号：US20060127887A1
公开(公告)日：2006-06-15
申请号：US11191221
申请日：2005-07-27
申请人： Young-sun Lee , Jung-im Han , Hyo-yeon Lee , Kak Namkoong , Kwang-wook Oh
发明人： Young-sun Lee , Jung-im Han , Hyo-yeon Lee , Kak Namkoong , Kwang-wook Oh
IPC分类号： C12Q1/70 , C12Q1/68 , C12N1/20 , C12N5/00 , C07K1/26
CPC分类号： C12N15/1006
摘要： Provided is a method of isolating and purifying biomolecules using a hydrogel, the method including: bring a sample containing charged biomolecules into contact with a hydrogel to bind the biomolecules to the hydrogel; washing the hydrogel bound with the biomolecules; and eluting the bound biomolecules using an elution solvent. According to the method, the use of a hydrogel with a large surface area reduces the isolation time of biomolecules to 5 min or less, an external device such as an electromagnet is not required, and small-sized systems or LOC can be easily implemented due to applicability to microsystems through a polymer patterning technique.

8. 发明授权

US07517690B2 Cell lysis method using free radical 有权
标题翻译：细胞裂解法使用自由基
公开(公告)号：US07517690B2
公开(公告)日：2009-04-14
申请号：US11291053
申请日：2005-11-30
申请人： Jung-im Han , Young-sun Lee , Kak Namkoong , Kwang-wook Oh
发明人： Jung-im Han , Young-sun Lee , Kak Namkoong , Kwang-wook Oh
IPC分类号： G01N33/48
CPC分类号： C12N1/06 , Y10T436/107497 , Y10T436/206664 , Y10T436/25 , Y10T436/2575
摘要： Provided is a method of lysing a cell or a virus using a free radical. The method includes: applying an electric field to a mixture of a metal ion, a peroxide, and a cell or virus solution to increase the free radical generation, thereby lysing a cell or a virus. In the present method, cell lysis may be efficiently performed using a low electrical energy (several mV to several V). When the present method is applied to a microsystem, cell lysis can occur at a desired time and in a desired space by controlling the electrical energy, thus being suitable to realize a lab-on-a-chip (LOC).
摘要翻译：提供了使用自由基裂解细胞或病毒的方法。该方法包括：向金属离子，过氧化物和细胞或病毒溶液的混合物施加电场以增加自由基产生，从而裂解细胞或病毒。在本方法中，可以使用低电能（几mV至几V）有效地进行细胞裂解。当本方法应用于微系统时，细胞裂解可以通过控制电能在期望的时间和期望的空间中发生，从而适于实现芯片上的实验室（LOC）。

9. 发明申请

US20060115902A1 Cell lysis method using free radical 有权
公开(公告)号：US20060115902A1
公开(公告)日：2006-06-01
申请号：US11291053
申请日：2005-11-30
申请人： Jung-im Han , Young-sun Lee , Kak Namkoong , Kwang-wook Oh
发明人： Jung-im Han , Young-sun Lee , Kak Namkoong , Kwang-wook Oh
IPC分类号： G01N1/10
CPC分类号： C12N1/06 , Y10T436/107497 , Y10T436/206664 , Y10T436/25 , Y10T436/2575
摘要： Provided is a method of lysing a cell or a virus using a free radical. The method includes: applying an electric field to a mixture of a metal ion, a peroxide, and a cell or virus solution to increase the free radical generation, thereby lysing a cell or a virus. In the present method, cell lysis may be efficiently performed using a low electrical energy (several mV to several V). When the present method is applied to a microsystem, cell lysis can occur at a desired time and in a desired space by controlling the electrical energy, thus being suitable to realize a lab-on-a-chip (LOC).

10. 发明授权

US08052855B2 Carbon nanotube gas sensor and method of manufacturing the same 有权
标题翻译：碳纳米管气体传感器及其制造方法
公开(公告)号：US08052855B2
公开(公告)日：2011-11-08
申请号：US11745522
申请日：2007-05-08
申请人： Jung-im Han , Soo-hyung Choi , Jeong-hee Lee , Soo-suk Lee , Jeong-na Heo
发明人： Jung-im Han , Soo-hyung Choi , Jeong-hee Lee , Soo-suk Lee , Jeong-na Heo
IPC分类号： G01N27/403 , G01N27/414
CPC分类号： G01N27/127 , Y10S977/742 , Y10S977/743 , Y10S977/953 , Y10S977/957
摘要： A carbon nanotube (“CNT”) gas sensor includes a substrate, an insulating layer formed on the substrate, electrodes formed on the insulating layer, and CNT barriers that protrude higher than the electrodes in spaces between the electrodes to form gas detecting spaces. A method of manufacturing the gas sensor includes forming an insulating layer on a substrate, forming an electrode pattern on the insulating layer, coating CNT paste having a thickness greater than a thickness of electrodes in the electrode pattern on the electrodes and the insulating layer, and patterning and firing the carbon nanotube paste, including using a photolithography method, to retain only portions of the CNT paste coated on spaces between the electrodes.
摘要翻译：碳纳米管（“CNT”）气体传感器包括基板，形成在基板上的绝缘层，形成在绝缘层上的电极和在电极之间的空间中比电极突出的CNT势垒，以形成气体检测空间。制造气体传感器的方法包括在基板上形成绝缘层，在绝缘层上形成电极图案，在电极和绝缘层上涂覆厚度大于电极图案的电极厚度的CNT浆料，以及包括使用光刻法构图和烧制碳纳米管糊，仅保留涂覆在电极之间的间隙上的部分CNT浆料。

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