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1. 发明授权

US5773299A Methods for the rapid analysis of the reticulocytes 失效
标题翻译：方法快速分析网织红细胞
公开(公告)号：US5773299A
公开(公告)日：1998-06-30
申请号：US777727
申请日：1996-12-20
申请人： Young Ran Kim , Johanna Kantor
发明人： Young Ran Kim , Johanna Kantor
IPC分类号： G01N33/48 , G01N1/30 , G01N15/14 , G01N33/49 , G01N33/80
CPC分类号： G01N33/80 , G01N15/1459 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , Y10S436/80 , Y10T436/10 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331
摘要： A method and reagent for the simultaneous or independent enumeration of reticulocytes in a whole blood sample, without the need to separately incubate the sample and reagent. The reagent contains a reticulocyte staining amount of an unsymmetrical cyanine dye, from about 40 mM to about 60 mM of a buffer selected from the group consisting of imidazole, Tris and Bis-Tris and a dye stabilizing amount of a non-ionic surfactant selected from the group consisting of N, N-bis�3-D-Glucon-amidopropyl! cholamide and a polyoxypropylene-polyoxyethylene block copolymer. The reagent has a pH from about 6.8 to about 7.2 and an osmolarity adjusted to about 280 to about 310 mosm/l with a mono-, or di-, valent alkali salt selected from the group consisting of NaCl, KCl, LiCl, CaCl.sub.2, MgCl.sub.2 and ZnCl.sub.2. The method utilizes the reagent in a no incubation process that also allows for the simultaneous determination of CBC as well as reticulocyte counts and maturity indices.
摘要翻译：一种用于在全血样品中同时或独立计数网织红细胞的方法和试剂，而不需要分别培养样品和试剂。试剂含有网织红细胞染色量的不对称花青染料，约40mM至约60mM的选自咪唑，Tris和Bis-Tris的缓冲液和染料稳定量的非离子表面活性剂，其选自由N，N-双[3-D-Glucon-amidopropyl]胆酰胺和聚氧丙烯 - 聚氧乙烯嵌段共聚物组成的组。试剂的pH为约6.8至约7.2，渗透压调节至约280至约310 mosm / l，其中选自NaCl，KCl，LiCl，CaCl 2， MgCl2和ZnCl2。该方法在无孵育过程中使用试剂，其也允许同时测定CBC以及网织红细胞计数和成熟度指数。

2. 发明授权

US5691204A Compositions and methods for the rapid analysis of reticulocytes 失效
标题翻译：用于快速分析网织红细胞的组合物和方法
公开(公告)号：US5691204A
公开(公告)日：1997-11-25
申请号：US426408
申请日：1995-04-21
申请人： Young Ran Kim , Johanna Kantor
发明人： Young Ran Kim , Johanna Kantor
IPC分类号： G01N33/48 , G01N1/30 , G01N15/14 , G01N33/49 , G01N33/80
CPC分类号： G01N33/80 , G01N15/1459 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , Y10S436/80 , Y10T436/10 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331
摘要： A method and reagent for the simultaneous or independent enumeration of reticulocytes in a whole blood sample, without the need to separately incubate the sample and reagent. The reagent contains a reticulocyte staining amount of an unsymmetrical cyanine dye, from about 40 mM to about 60 mM of a buffer selected from the group consisting of imidazole, Tris and Bis-Tris and a dye stabilizing amount of a non-ionic surfactant selected from the group consisting of N, N-bis�3-D-Glucon-amidopropyl! cholamide and a polyoxypropylene-polyoxyethylene block copolymer. The reagent has a pH from about 6.8 to about 7.2 and an osmolarity adjusted to about 280 to about 310 mOsm/l with a mono-, or di-, valent alkali salt selected from the group consisting of NaCl, KCl, LiCl, CaCl.sub.2, MgCl.sub.2 and ZnCl.sub.2. The method utilizes the reagent in a no incubation process that also allows for the simultaneous determination of CBC as well as reticulocyte counts and maturity indices.
摘要翻译：一种用于在全血样品中同时或独立计数网织红细胞的方法和试剂，而不需要分别培养样品和试剂。试剂含有网织红细胞染色量的不对称花青染料，约40mM至约60mM的选自咪唑，Tris和Bis-Tris的缓冲液和染料稳定量的非离子表面活性剂，其选自由N，N-双[3-D-Glucon-amidopropyl]胆酰胺和聚氧丙烯 - 聚氧乙烯嵌段共聚物组成的组。试剂的pH为约6.8至约7.2，渗透压调节至约280至约310mOsm / l，选自NaCl，KCl，LiCl，CaCl 2， MgCl2和ZnCl2。该方法在无孵育过程中使用试剂，其也允许同时测定CBC以及网织红细胞计数和成熟度指数。

3. 发明授权

US5648225A Method of using a multi-purpose beagent for subclassification of nucleated blood cells 失效
标题翻译：使用多用途试剂进行有核血细胞分类的方法
公开(公告)号：US5648225A
公开(公告)日：1997-07-15
申请号：US296379
申请日：1994-08-25
申请人： Young Ran Kim , Johanna Kantor , James E. Gill , Sue E. Luptovic
发明人： Young Ran Kim , Johanna Kantor , James E. Gill , Sue E. Luptovic
IPC分类号： G01N33/48 , A01N1/02 , A61K49/00 , C12N1/06 , C12Q1/02 , G01N1/28 , G01N1/30 , G01N15/14 , G01N33/49 , G01N33/50 , G01N33/569 , G01N33/80
CPC分类号： C12N1/06 , G01N15/1459 , G01N33/5094 , G01N33/56972 , G01N33/80 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , Y10S436/805 , Y10S436/808 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331 , Y10T436/25
摘要： A multipurpose reagent system for rapid analysis of a whole blood sample allowing the determination of at least five classes of peripheral white blood cells, nucleated red blood cells, and lymphocyte immunophenotyping on automated hematology instrumentation. The multipurpose reagent system lyses red cells rapidly, while it concurrently fixes white cells and preserves surface antigens on lymphocytes. The multipurpose reagent system comprises from about 3 to 7 grams per liter of a non-quaternary ammonium salt, from about 0.04 to about 0.10 percent by volume of an aliphatic aldehyde with one to four carbons, from about 10 mM to about 20 mM of a non-phosphate buffer which is inert to the aliphatic aldehyde, and a sufficient amount of water to give a pH between 5.5 and 7.5 and an osmolality of between about 160 to about 310 mOsm per liter.
摘要翻译：用于快速分析全血样品的多用途试剂系统，允许在自动血液仪器上测定至少五类外周白细胞，有核红细胞和淋巴细胞免疫表型分析。多用途试剂系统迅速溶解红细胞，同时固定白细胞并保留淋巴细胞上的表面抗原。多用途试剂系统包含约3至7克/升非季铵盐，约0.04至约0.10体积％的具有一至四个碳的脂族醛，约10mM至约20mM的对脂族醛是惰性的非磷酸盐缓冲剂，以及足够量的水以产生5.5至7.5之间的pH和约160至约310mOsm /升的渗透压。

4. 发明授权

US5516695A Multipurpose reagent system for rapid lysis of whole blood 失效
标题翻译：多用途试剂系统，用于全血快速溶解
公开(公告)号：US5516695A
公开(公告)日：1996-05-14
申请号：US297662
申请日：1994-08-29
申请人： Young R. Kim , Johanna Kantor , James E. Gill , Sue E. Luptovic
发明人： Young R. Kim , Johanna Kantor , James E. Gill , Sue E. Luptovic
IPC分类号： G01N33/48 , A01N1/02 , A61K49/00 , C12N1/06 , C12Q1/02 , G01N1/28 , G01N1/30 , G01N15/14 , G01N33/49 , G01N33/50 , G01N33/569 , G01N33/80
CPC分类号： C12N1/06 , G01N15/1459 , G01N33/5094 , G01N33/56972 , G01N33/80 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , Y10S436/805 , Y10S436/808 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331 , Y10T436/25
摘要： A multipurpose reagent system for rapid analysis of a whole blood sample allowing the determination of at least five classes of peripheral white blood cells, nucleated red blood cells, and lymphocyte immunophenotyping on automated hematology instrumentation. The multipurpose reagent system lyses red cells rapidly, while it concurrently fixes white cells and preserves surface antigens on lymphocytes. The multipurpose reagent system comprises from about 3 to 7 grams per liter of a non-quaternary ammonium salt, from about 0.04 to about 0.10 percent by volume of an aliphatic aldehyde with one to four carbons, from about 10 mM to about 20 mM of a non-phosphate buffer which is inert to the aliphatic aldehyde, and a sufficient amount of water to give a pH between 5.5 and 7.5 and an osmolality of between about 160 to about 310 mOsm per liter.
摘要翻译：用于快速分析全血样品的多用途试剂系统，允许在自动血液仪器上测定至少五类外周白细胞，有核红细胞和淋巴细胞免疫表型分析。多用途试剂系统迅速溶解红细胞，同时固定白细胞并保留淋巴细胞上的表面抗原。多用途试剂系统包含约3至7克/升非季铵盐，约0.04至约0.10体积％的具有一至四个碳的脂族醛，约10mM至约20mM的对脂族醛是惰性的非磷酸盐缓冲剂，以及足够量的水以产生5.5至7.5之间的pH和约160至约310mOsm /升的渗透压。

5. 外观设计

USD818748S1 Sample rack 有权
公开(公告)号：USD818748S1
公开(公告)日：2018-05-29
申请号：US29598505
申请日：2017-03-27
申请人： William F. Stewart , Johanna Kantor
设计人： William F. Stewart , Johanna Kantor

6. 发明授权

US5559037A Method for rapid and simultaneous analysis of nucleated red blood cells 失效
标题翻译：快速同时分析有核红细胞的方法
公开(公告)号：US5559037A
公开(公告)日：1996-09-24
申请号：US356932
申请日：1994-12-15
申请人： Young R. Kim , Michael W. Yee , Suresh N. Mehta , Josefino C. Sagala , Johanna Kantor
发明人： Young R. Kim , Michael W. Yee , Suresh N. Mehta , Josefino C. Sagala , Johanna Kantor
IPC分类号： G01N21/64 , G01N15/00 , G01N15/14 , G01N21/53 , G01N33/49 , G01N33/48
CPC分类号： G01N15/1456 , Y10T436/101666 , Y10T436/107497 , Y10T436/25125
摘要： A method and a device for the simultaneous and quantitative, flow cytometric analysis of nucleated red blood cells (NRBC) and white blood cells (WBC) in a whole blood sample. The method includes the lysis of red blood cells (RBC) and NRBC cytoplasm from an aliquot of a whole blood sample to eliminate RBC and to expose the NRBC to a vital nuclear stain and the inhibition of the permeation of the nuclear stain into the WBC, subjecting the stained aliquot to flow cytometric light measurements, obtaining at least one signal for parameters including scattered light at a first and a second range of scatter angles and fluorescence (FL), qualifying the obtained signals by using the combination logic wherein a qualified signal must be greater than the second scatter signal threshold, while at the same time it must be greater than either the first scatter signal threshold or the FL threshold {[(first scatter angle signal OR FL signals) AND second scatter angle signal]}, constructing a three-dimensional plot of qualified intensity signals of fluorescence and scattered light from the detected signals, and differentiating the NRBC and WBC from the constructed three-dimensional plot and determining the number of cells of each.
摘要翻译：一种用于全血样品中有核红细胞（NRBC）和白细胞（WBC）的同时和定量流式细胞术分析的方法和装置。该方法包括从全血样品的等分试样中分离红细胞（RBC）和NRBC细胞质，以消除RBC并将NRBC暴露于重要的核染色和抑制核染料渗透到WBC中，对染色的等分试样进行流式细胞计数测量，获得至少一个参数信号，包括在第一和第二散射角范围内的散射光和荧光（FL），通过使用组合逻辑来限定所获得的信号，其中合格信号必须大于第二散射信号阈值，而同时它必须大于第一散射信号阈值或FL阈值{[（第一散射角信号OR FL信号）AND第二散射角信号]}，构成来自检测信号的荧光和散射光的合格强度信号的三维图，并将NRBC和WBC与构建的三分之一区分开来确定每个细胞的数量。

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