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1. 发明授权

US5648225A Method of using a multi-purpose beagent for subclassification of nucleated blood cells 失效
标题翻译：使用多用途试剂进行有核血细胞分类的方法
公开(公告)号：US5648225A
公开(公告)日：1997-07-15
申请号：US296379
申请日：1994-08-25
申请人： Young Ran Kim , Johanna Kantor , James E. Gill , Sue E. Luptovic
发明人： Young Ran Kim , Johanna Kantor , James E. Gill , Sue E. Luptovic
IPC分类号： G01N33/48 , A01N1/02 , A61K49/00 , C12N1/06 , C12Q1/02 , G01N1/28 , G01N1/30 , G01N15/14 , G01N33/49 , G01N33/50 , G01N33/569 , G01N33/80
CPC分类号： C12N1/06 , G01N15/1459 , G01N33/5094 , G01N33/56972 , G01N33/80 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , Y10S436/805 , Y10S436/808 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331 , Y10T436/25
摘要： A multipurpose reagent system for rapid analysis of a whole blood sample allowing the determination of at least five classes of peripheral white blood cells, nucleated red blood cells, and lymphocyte immunophenotyping on automated hematology instrumentation. The multipurpose reagent system lyses red cells rapidly, while it concurrently fixes white cells and preserves surface antigens on lymphocytes. The multipurpose reagent system comprises from about 3 to 7 grams per liter of a non-quaternary ammonium salt, from about 0.04 to about 0.10 percent by volume of an aliphatic aldehyde with one to four carbons, from about 10 mM to about 20 mM of a non-phosphate buffer which is inert to the aliphatic aldehyde, and a sufficient amount of water to give a pH between 5.5 and 7.5 and an osmolality of between about 160 to about 310 mOsm per liter.
摘要翻译：用于快速分析全血样品的多用途试剂系统，允许在自动血液仪器上测定至少五类外周白细胞，有核红细胞和淋巴细胞免疫表型分析。多用途试剂系统迅速溶解红细胞，同时固定白细胞并保留淋巴细胞上的表面抗原。多用途试剂系统包含约3至7克/升非季铵盐，约0.04至约0.10体积％的具有一至四个碳的脂族醛，约10mM至约20mM的对脂族醛是惰性的非磷酸盐缓冲剂，以及足够量的水以产生5.5至7.5之间的pH和约160至约310mOsm /升的渗透压。

2. 发明授权

US5516695A Multipurpose reagent system for rapid lysis of whole blood 失效
标题翻译：多用途试剂系统，用于全血快速溶解
公开(公告)号：US5516695A
公开(公告)日：1996-05-14
申请号：US297662
申请日：1994-08-29
申请人： Young R. Kim , Johanna Kantor , James E. Gill , Sue E. Luptovic
发明人： Young R. Kim , Johanna Kantor , James E. Gill , Sue E. Luptovic
IPC分类号： G01N33/48 , A01N1/02 , A61K49/00 , C12N1/06 , C12Q1/02 , G01N1/28 , G01N1/30 , G01N15/14 , G01N33/49 , G01N33/50 , G01N33/569 , G01N33/80
CPC分类号： C12N1/06 , G01N15/1459 , G01N33/5094 , G01N33/56972 , G01N33/80 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , Y10S436/805 , Y10S436/808 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331 , Y10T436/25
摘要： A multipurpose reagent system for rapid analysis of a whole blood sample allowing the determination of at least five classes of peripheral white blood cells, nucleated red blood cells, and lymphocyte immunophenotyping on automated hematology instrumentation. The multipurpose reagent system lyses red cells rapidly, while it concurrently fixes white cells and preserves surface antigens on lymphocytes. The multipurpose reagent system comprises from about 3 to 7 grams per liter of a non-quaternary ammonium salt, from about 0.04 to about 0.10 percent by volume of an aliphatic aldehyde with one to four carbons, from about 10 mM to about 20 mM of a non-phosphate buffer which is inert to the aliphatic aldehyde, and a sufficient amount of water to give a pH between 5.5 and 7.5 and an osmolality of between about 160 to about 310 mOsm per liter.
摘要翻译：用于快速分析全血样品的多用途试剂系统，允许在自动血液仪器上测定至少五类外周白细胞，有核红细胞和淋巴细胞免疫表型分析。多用途试剂系统迅速溶解红细胞，同时固定白细胞并保留淋巴细胞上的表面抗原。多用途试剂系统包含约3至7克/升非季铵盐，约0.04至约0.10体积％的具有一至四个碳的脂族醛，约10mM至约20mM的对脂族醛是惰性的非磷酸盐缓冲剂，以及足够量的水以产生5.5至7.5之间的pH和约160至约310mOsm /升的渗透压。

3. 发明授权

US5891734A Method for performing automated analysis 失效
标题翻译：执行自动分析的方法
公开(公告)号：US5891734A
公开(公告)日：1999-04-06
申请号：US682334
申请日：1996-07-17
申请人： James E. Gill , Vernon L. Chupp , Luc Van Hove
发明人： James E. Gill , Vernon L. Chupp , Luc Van Hove
IPC分类号： G01N33/536 , B01F5/04 , G01N1/00 , G01N15/00 , G01N15/14 , G01N35/00 , G01N35/10 , G01N33/533
CPC分类号： G01N35/1095 , B01F5/0453 , B01F5/046 , G01N15/14 , G01N15/1404 , G01N35/0092 , G01N35/1004 , G01N35/109 , G01N1/14 , G01N15/1459 , G01N2015/0069 , G01N2015/0076 , G01N2015/0084 , G01N2015/1413 , G01N2035/00356 , G01N2035/00524 , G01N2035/1032 , G01N35/10 , Y10S435/973 , Y10S436/805 , Y10T436/11 , Y10T436/25 , Y10T436/25125 , Y10T436/2575
摘要： Provided are automated methods for distinguishing and differentiating cells in a whole blood sample. In one of the methods, a whole blood sample is provided. One or more tests to be performed on the whole blood sample is selected. The tests to be performed on the whole blood sample are correlated. A volume of the whole blood sample is aspirated into an automated instrument system which automatically performs conventional hematology analysis and fluorescent cytometry analysis on the whole blood sample. A first aliquot of the whole blood sample is dispensed into at least one sample receiving vessel. The first aliquot of the whole blood sample is mixed with a fluorescent reagent. The first aliquot of the whole blood sample mixed with fluorescent reagent is diluted and transported through a flow transducer system. The flow transducer system detects multi-angle light scatter and fluorescence from the first aliquot of the whole blood sample mixed with fluorescent reagent and counts and differentiates platelets or platelet clumps or both in the sample. Detecting and differentiation data for the one or more tests performed on the whole blood sample are stored. Results of the one or more tests performed on the whole blood sample are reported in a quantitative manner if so requested. The instrument system automatically performs all method steps without physically separating cells from the whole blood sample or an aliquot of the sample and results of a conventional hematology analysis may be utilized in at least reporting of results of the fluorescent cytometry testing.
摘要翻译：提供了用于区分和分化全血样品中的细胞的自动化方法。在其中一种方法中，提供了全血样品。选择对全血样进行一次或多次测试。对全血样进行的检测是相关的。将全血样品的体积吸入自动化仪器系统中，自动进行全血样品的常规血液学分析和荧光细胞分析。将全血样品的第一等分试样分配到至少一个样品接收容器中。将全血样品的第一等份与荧光试剂混合。与荧光试剂混合的全血样品的第一等份被稀释并通过流量传感器系统传输。流量传感器系统检测来自与荧光试剂混合的全血样品的第一等分试样的多角度光散射和荧光，并对样品中的血小板或血小板结块或两者进行计数和微分。存储对全血样品进行的一次或多次测试的检测和分化数据。如果要求，则以定量方式报告对全血样品进行的一项或多项测试的结果。仪器系统自动执行所有方法步骤，而不用从全血样品或样品的等分试样物质分离细胞，并且常规血液学分析的结果可以用于至少报告荧光细胞测定结果。

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