专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5559037A Method for rapid and simultaneous analysis of nucleated red blood cells 失效
标题翻译：快速同时分析有核红细胞的方法
公开(公告)号：US5559037A
公开(公告)日：1996-09-24
申请号：US356932
申请日：1994-12-15
申请人： Young R. Kim , Michael W. Yee , Suresh N. Mehta , Josefino C. Sagala , Johanna Kantor
发明人： Young R. Kim , Michael W. Yee , Suresh N. Mehta , Josefino C. Sagala , Johanna Kantor
IPC分类号： G01N21/64 , G01N15/00 , G01N15/14 , G01N21/53 , G01N33/49 , G01N33/48
CPC分类号： G01N15/1456 , Y10T436/101666 , Y10T436/107497 , Y10T436/25125
摘要： A method and a device for the simultaneous and quantitative, flow cytometric analysis of nucleated red blood cells (NRBC) and white blood cells (WBC) in a whole blood sample. The method includes the lysis of red blood cells (RBC) and NRBC cytoplasm from an aliquot of a whole blood sample to eliminate RBC and to expose the NRBC to a vital nuclear stain and the inhibition of the permeation of the nuclear stain into the WBC, subjecting the stained aliquot to flow cytometric light measurements, obtaining at least one signal for parameters including scattered light at a first and a second range of scatter angles and fluorescence (FL), qualifying the obtained signals by using the combination logic wherein a qualified signal must be greater than the second scatter signal threshold, while at the same time it must be greater than either the first scatter signal threshold or the FL threshold {[(first scatter angle signal OR FL signals) AND second scatter angle signal]}, constructing a three-dimensional plot of qualified intensity signals of fluorescence and scattered light from the detected signals, and differentiating the NRBC and WBC from the constructed three-dimensional plot and determining the number of cells of each.
摘要翻译：一种用于全血样品中有核红细胞（NRBC）和白细胞（WBC）的同时和定量流式细胞术分析的方法和装置。该方法包括从全血样品的等分试样中分离红细胞（RBC）和NRBC细胞质，以消除RBC并将NRBC暴露于重要的核染色和抑制核染料渗透到WBC中，对染色的等分试样进行流式细胞计数测量，获得至少一个参数信号，包括在第一和第二散射角范围内的散射光和荧光（FL），通过使用组合逻辑来限定所获得的信号，其中合格信号必须大于第二散射信号阈值，而同时它必须大于第一散射信号阈值或FL阈值{[（第一散射角信号OR FL信号）AND第二散射角信号]}，构成来自检测信号的荧光和散射光的合格强度信号的三维图，并将NRBC和WBC与构建的三分之一区分开来确定每个细胞的数量。

2. 发明授权

US5879900A Method for simultaneous analysis of cell viability, nucleated red blood cells and white blood cell differentials 失效
标题翻译：同时分析细胞活力，有核红细胞和白细胞差异的方法
公开(公告)号：US5879900A
公开(公告)日：1999-03-09
申请号：US851526
申请日：1997-05-05
申请人： Young Ran Kim , Michael W. Yee , Suresh N. Mehta , Josefino C. Sagala
发明人： Young Ran Kim , Michael W. Yee , Suresh N. Mehta , Josefino C. Sagala
IPC分类号： G01N15/00 , G01N15/14 , G01N33/569
CPC分类号： G01N15/1459 , G01N2015/1006 , G01N2015/1402
摘要： A method for the simultaneous and quantitative, flow cytometric analysis of nucleated red blood cells (NRBC), white blood cells (WBC), damaged white blood cells and a white blood cell subclass differential (WBC/Diff) is provided. The method includes mixing an aliquot of a whole blood sample with a reagent system comprising a red blood cell (RBC) lysing component which lyses RBCs and NRBCs while minimizing damage to WBC cellular membranes and a membrane-impermeant nucleic acid stain which stains exposed NRBC nuclei and damaged WBCs, subjecting the stained aliquot to flow cytometric light measurements, obtaining at least one signal for the parameters of fluorescence (FL) and scattered light at a first and a second range of scatter angles, qualifying the obtained signals using AND/OR logic wherein to be qualified, a signal must be greater than the second scatter signal threshold AND also greater than either the first scatter signal threshold OR the FL threshold, constructing a three-dimensional plot of qualified intensity signals of fluorescence and scattered light from the detected signals, and differentiating the NRBC, WBC, damaged WBC and WBC/Diff from the constructed three-dimensional plot and determining the number of cells of each.
摘要翻译：提供了有色红细胞（NRBC），白细胞（WBC），受损白细胞和白血细胞亚类差异（WBC / Diff）的同步和定量流式细胞术分析方法。该方法包括将全血样品的等分试样与包含红细胞（RBC）裂解组分的试剂系统混合，所述红细胞裂解组分裂解RBC和NRBC，同时使对WBC细胞膜的损伤最小化，以及污染暴露的NRBC核的膜不透核酸染色和损坏的WBC，染色的等分试样流式细胞计数测量，在第一和第二散射角范围内获得荧光参数（FL）和散射光的至少一个信号，使用AND / OR逻辑其中要被限定，信号必须大于第二散射信号阈值AND，并且还大于第一散射信号阈值OR FL阈值，构成来自检测信号的荧光和散射光的合格强度信号的三维图，并从构造的三维图中区分NRBC，WBC，损坏的WBC和WBC / Diff，并确定每个细胞数。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式