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1. 发明授权

US4459213A Fire-extinguisher composition 失效
标题翻译：灭火器组成
公开(公告)号：US4459213A
公开(公告)日：1984-07-10
申请号：US454594
申请日：1982-12-30
申请人： Yasuzo Uchida , Sadayuki Kawai , Koichi Sugita , Hitoshi Nagasaki , Yoichi Shirakawa
发明人： Yasuzo Uchida , Sadayuki Kawai , Koichi Sugita , Hitoshi Nagasaki , Yoichi Shirakawa
IPC分类号： A62D1/02 , A62D1/00
CPC分类号： A62D1/0078
摘要： A fire-extinguisher composition comprising a blend of at least one member selected from the group consisting of protein and protein decomposition products, at least one liquid polyhydroxy compound or an aqueous solution of at least one polyhydroxy compound, and at least one halogenated hydrocarbon. The composition can easily be emulsified or dispersed in water and exhibits a high retention of its fire-extinguishing ability, a satisfactory secondary fire-extinguishing ability, and a foaming ability sufficient to make a foaming device unnecessary.
摘要翻译：一种灭火剂组合物，其包含选自蛋白质和蛋白质分解产物中的至少一种的成分的混合物，至少一种液体多羟基化合物或至少一种多羟基化合物的水溶液和至少一种卤代烃。组合物可以容易地乳化或分散在水中，并且表现出高的保持其灭火能力，令人满意的二次灭火能力和足以使发泡装置不必要的发泡能力。

2. 发明授权

US06319225B1 Injection syringe including device for preparation of injection 有权
标题翻译：注射器包括注射装置
公开(公告)号：US06319225B1
公开(公告)日：2001-11-20
申请号：US09266916
申请日：1999-03-12
申请人： Koichi Sugita , Sei Kirihara , Hiromichi Ishikawa , Yutaka Igarashi
发明人： Koichi Sugita , Sei Kirihara , Hiromichi Ishikawa , Yutaka Igarashi
IPC分类号： A61M3700
CPC分类号： A61M5/2448 , A61M5/31553 , A61M5/31591
摘要： An injection syringe including a device for preparation of an injection in situ, useful to the injection which is liable to suffer chemical changes if left for a long time in the state of solution or dispersion ready to inject. The injection syringe is portable by a patient who may prepare a necessary injection in situ with the use of a device included in the syringe which will automatically perform an injection with a prescribed dosage. The injection syringe includes a device for preparation of an injection, whereby mechanical impacts affecting the medicine and consequently, chemical changes with a medicine would be minimized during the step of dissolving the medicine. The syringe as noted is especially useful for preparation of injection and injection of human growth hormones, interferon and various polypeptides which are of an environmentally sensitive nature and liable to suffer chemical changes if left for a long time in the state of solution or dispersion.
摘要翻译：一种注射器，其包括用于制备原位注射的装置，其用于注射，如果在准备注射的溶液或分散体的状态下长时间存在易于发生化学变化。注射器可以由患者便携，该患者可以使用包括在注射器中的装置在现场准备必要的注射器，其将以规定剂量自动进行注射。注射器包括用于制备注射器的装置，由此在溶解药物的步骤期间，影响药物的机械冲击以及因此的药物的化学变化将被最小化。如上所述，注射器特别适用于制备注射和注射人生长激素，干扰素和各种具有环境敏感性质的多肽，并且如果在溶液或分散状态下长时间使用，则易于遭受化学变化。

3. 发明授权

US5085350A Pump assembly with container for storing liquid in isolation from ambient air 失效
标题翻译：泵组件带有用于与环境空气隔离的液体的容器
公开(公告)号：US5085350A
公开(公告)日：1992-02-04
申请号：US494534
申请日：1990-03-16
申请人： Koichi Sugita
发明人： Koichi Sugita
IPC分类号： B05B11/00
CPC分类号： B05B11/0048 , B05B11/0056 , B05B11/0097 , B05B11/3049 , B05B11/3026
摘要： A pump assembly for storing and discharging a liquid such as a chemical solution includes a substantially cylindrical container for storing the liquid therein. A pump is mounted on one end of the container. The pump has a suction port for drawing the liquid from the container and an outlet port for discharging the liquid. A gasket is slidably inserted in the opposite end of the container in contact with the liquid stored in the container, for sealing the liquid in the container. The gasket is slidable toward the pump when the liquid stored in the container is reduced by the pump. A slanted portion is disposed in one end of the container and has a slanted surface extending continuously from an inner peripheral surface of the container and converging away from the gasket. The suction port of the pump communicates with the interior space of the container substantially at a converging end of the slanted surface. Any trapped air is collected between the slanted surface and the liquid contained in the container, and can be discharged from the container by the pump before the liquid is discharged from the container by the pump.
摘要翻译：用于储存和排出诸如化学溶液的液体的泵组件包括用于在其中储存液体的基本上圆柱形的容器。泵安装在容器的一端。泵具有用于从容器抽出液体的抽吸口和用于排出液体的出口。垫圈可滑动地插入与容器中存储的液体接触的容器的相对端，用于密封容器中的液体。当储存在容器中的液体被泵减少时，垫圈可朝向泵滑动。倾斜部分设置在容器的一端，并且具有从容器的内周面连续延伸的倾斜表面，并从垫圈收敛。泵的吸入口基本上在倾斜表面的收敛端与容器的内部空间连通。任何被捕获的空气被收集在倾斜表面和容纳在容器中的液体之间，并且在液体通过泵从容器排出之前可以通过泵从容器中排出。

4. 发明申请

US20120054923A1 METHOD FOR CULTIVATION OF GENETICALLY-MODIFIED PLANT 有权
标题翻译：基因改造植物的培育方法
公开(公告)号：US20120054923A1
公开(公告)日：2012-03-01
申请号：US13147436
申请日：2009-08-26
申请人： Saori Kasahara , Masafumi Wasai , Koichi Sugita , Teruhisa Shimada
发明人： Saori Kasahara , Masafumi Wasai , Koichi Sugita , Teruhisa Shimada
IPC分类号： A01H5/10 , A01G31/00 , A01H5/00
CPC分类号： C12N15/8261 , C12N15/8234 , C12N15/8238 , C12N15/8251 , C12N15/8257 , Y02A40/146
摘要： The present invention provides a method for cultivation of a genetically-modified plant that can highly produce a desired protein. More specifically, the present invention provides a method for cultivation of a genetically-modified plant comprising: cultivating the genetically-modified plant in a medium, wherein the genetically-modified plant is transformed by introducing an expression vector comprising a promoter regulating expression of RNA and a seed storage protein isolated from a plant that highly expresses RNA and the seed storage protein under a high nitrogen condition; and a polynucleotide encoding an objective protein, and wherein the medium is adjusted so that nitrate nitrogen is 70 mg/L to 750 mg/L and/or ammonium nitrogen is 70 mg/L to 750 mg/L for a definite period in a period from 30 days before an expected flowering date to a date on or before flowering of the genetically-modified plant.
摘要翻译：本发明提供了能够高度产生所需蛋白质的遗传修饰植物的培养方法。更具体地说，本发明提供了一种遗传修饰植物的培养方法，包括：在培养基中培养经遗传修饰的植物，其中转基因修饰植物通过引入包含调节RNA表达的启动子的表达载体和在高氮条件下从高度表达RNA和种子储存蛋白的植物中分离的种子储存蛋白; 和编码目标蛋白质的多核苷酸，并且调节培养基使得硝酸盐氮在70mg / L至750mg / L和/或铵氮为70mg / L至750mg / L的条件下在一定期间内从预计开花日期前30天到转基因植物开花前后的日期。

5. 发明授权

US5150823A Combination container and pump having a conical piston for venting 失效
标题翻译：组合容器和泵具有用于通风的活塞式活塞
公开(公告)号：US5150823A
公开(公告)日：1992-09-29
申请号：US603618
申请日：1990-10-26
申请人： Koichi Sugita
发明人： Koichi Sugita
IPC分类号： B05B11/00 , B65D83/00
CPC分类号： B05B11/0048 , B05B11/0056 , B65D83/0033
摘要： A pump is mounted in one end of a cylindrical container for storing a liquid, for drawing the liquid stored in the cylindrical container and discharging the drawn liquid out of the cylindrical container. A piston is slidably disposed in the opposite end of the container in keeping the liquid sealed in the cylindrical container. The piston is movable toward the pump as the amount of the liquid stored in the cylindrical container is reduced when the liquid is discharged from the cylindrical container by the pump. The piston has a slanted surface facing the pump for contact with the liquid stored in the cylindrical container, the slanted surface being inclined progressively toward the second axial end. The piston also has a cylindrical surface defining a communication hole defined axially therethrough and having an inner end opening at the slanted surface. The communication hole provides communication between the interior and the exterior of the cylindrical container. A cap comprises a cap body inserted in the communication hole and a flange closes an outer end of the communication hole. At least either one of the cap body and the cylindrical surface has at least one axial groove. Air bubbles which may have been trapped in the container when the liquid is charged into the container move along slanted surface into the communication hole and then is escaped from the container through the groove.
摘要翻译：泵安装在用于储存液体的圆柱形容器的一端中，用于抽出存储在圆筒形容器中的液体并将抽出的液体排出圆筒形容器。活塞可滑动地设置在容器的相对端中，以保持液体密封在圆柱形容器中。当液体通过泵从圆筒形容器排出时，随着储存在圆筒形容器中的液体的量减少，活塞可朝向泵移动。活塞具有面向泵的倾斜表面，用于与存储在圆柱形容器中的液体接触，倾斜表面朝着第二轴向端逐渐倾斜。活塞还具有圆柱形表面，该圆柱形表面限定了轴向穿过其并且在倾斜表面具有内端开口的连通孔。连通孔提供圆柱形容器的内部和外部之间的连通。盖包括插入连通孔中的盖体，凸缘封闭连通孔的外端。盖主体和圆柱形表面中的至少一个具有至少一个轴向凹槽。当液体装入容器时可能被捕获在容器中的气泡沿倾斜的表面移动到连通孔中，然后通过凹槽从容器中逸出。

6. 发明申请

US20080292732A1 Plant and Plant Storage Organ Having Glp-1 Derivative Accumulated Therein and Method of Producing the Same 失效
标题翻译：含有Glp-1衍生物的植物和植物储存器官及其生产方法
公开(公告)号：US20080292732A1
公开(公告)日：2008-11-27
申请号：US11662650
申请日：2004-09-14
申请人： Koichi Sugita , Saori Kasahara , Hiroyasu Ebinuma , Fumio Takaiwa , Hiroshi Yasuda , Takahito Jomori , Yuji Hayashi , Akira Tashita
发明人： Koichi Sugita , Saori Kasahara , Hiroyasu Ebinuma , Fumio Takaiwa , Hiroshi Yasuda , Takahito Jomori , Yuji Hayashi , Akira Tashita
IPC分类号： A61K36/00 , C12N15/82 , A01H5/00 , A61P31/10
CPC分类号： A61K38/26 , A23L33/105 , C12N15/8257
摘要： The present invention is to provide a plant and a plant storage organ thereof in which GLP-1 derivatives are accumulated, and a method of producing them in order to develop a method for ingesting orally GLP-1 derivatives at a low cost and to make use of it in diabetic treatment. A transgenic plant or a plant storage organ thereof in which GLP-1 derivatives are accumulated cleavable with a digestive enzyme is produced by a method comprising: integrating into a vector a linked GLP-1s-DNA which comprises tandem repeated “n” DNAs (“n” being an integer of 3 or more) encoding a GLP-1 derivative consisting of GLP-1 (7-36) or of an amino acid sequence of GLP-1 (7-36) in which one or a few amino acids are deleted, substituted and/or added, and C-terminal consists of Arg or Lys, and having GLP-1 activity; introducing the vector into a plant cell; and redifferentiating the obtained transformant. The transgenic plant and the plant storage organ are useful as a pharmaceutical composition or a food or drink for treating or preventing diabetes.
摘要翻译：本发明提供一种其中积聚有GLP-1衍生物的植物和植物贮藏器官及其生产方法，以便开发低成本摄取口服GLP-1衍生物的方法并使用的糖尿病治疗。 GLP-1衍生物可以用消化酶积聚可切割的转基因植物或植物贮藏器官是通过以下方法产生的：将连接的GLP-1s-DNA整合到载体中，所述连接的GLP-1s-DNA包含串联重复的“n”DNA（“ 编号由GLP-1（7-36）或GLP-1（7-36）的氨基酸序列组成的GLP-1衍生物的n“为3以上的整数，其中一个或几个氨基酸为缺失，取代和/或添加，C-末端由Arg或Lys组成，具有GLP-1活性; 将载体导入植物细胞; 并对所得转化体进行重新分化。转基因植物和植物贮藏器官可用作药物组合物或用于治疗或预防糖尿病的食物或饮料。

7. 发明授权

US06326192B1 Vector for gene transfer into plant allowing optional deletion of marker gene 失效
标题翻译：用于基因转移到植物中的载体，允许标记基因的任选缺失
公开(公告)号：US06326192B1
公开(公告)日：2001-12-04
申请号：US09147241
申请日：1998-11-09
申请人： Koichi Sugita , Mikiko Uesugi , Etsuko Matsunaga , Hiroyasu Ebinuma
发明人： Koichi Sugita , Mikiko Uesugi , Etsuko Matsunaga , Hiroyasu Ebinuma
IPC分类号： C12N1582
CPC分类号： C12N15/8205 , C12N15/8201 , C12N15/8209 , C12N15/8213 , C12N15/8242
摘要： The present invention relates to a vector for introducing a desired gene into a plant, wherein a selectable marker gene introduced into a plant cell along with a desired gene is optionally removable from the DNA such as chromosome or the like where it exists and functions, then disappeared the function thereof after its expression, and the expression of the selectable marker gene and the disappearance of the function thereof are detectable by morphological change of the tissue derived from the plant cell into which the selectable marker gene is introduced. Also, the present invention constitutes a vector using a morphological abnormality induction gene as a selectable marker gene, while putting a removable DNA element under control of an inducible promoter, wherein the morphological abnormality induction gene is positioned such that it behaves integrally with the removable DNA element, and wherein a desired gene is positioned such that it does not behave integrally with the removable DNA element.
摘要翻译：本发明涉及用于将期望的基因导入植物中的载体，其中与所需基因一起导入植物细胞的选择性标记基因可任选地从其存在并起作用的染色体等DNA脱除，然后其表达后的功能消失，并且可选择标记基因的表达及其功能的消失可以通过导入选择性标记基因的植物细胞衍生的组织的形态学变化来检测。此外，本发明构成使用形态异常诱导基因作为选择性标记基因的载体，同时将可移除的DNA元件置于诱导型启动子的控制下，其中形态异常诱导基因定位成使其与可除去的DNA整体地起作用元件，并且其中将期望的基因定位成使得其不与可移除的DNA元件整体表现。

8. 发明授权

US5965791A Vector for introducing a gene into a plant, and methods for producing transgenic plants and multitudinously introducing genes into a plant using the vector 失效
标题翻译：用于将基因导入植物的载体，以及使用载体生产转基因植物并将基因大量引入植物的方法
公开(公告)号：US5965791A
公开(公告)日：1999-10-12
申请号：US555760
申请日：1995-11-09
申请人： Hiroyasu Ebinuma , Koichi Sugita , Etsuko Matsunaga , Mikiko Yamakado
发明人： Hiroyasu Ebinuma , Koichi Sugita , Etsuko Matsunaga , Mikiko Yamakado
IPC分类号： A01H1/00 , A01H5/00 , C07H21/04 , C12N1/21 , C12N5/00 , C12N5/10 , C12N15/09 , C12N15/29 , C12N15/52 , C12N15/82 , C12R1/01 , C12R1/91 , A01H4/00
CPC分类号： C12N15/821 , C12N15/8201 , C12N15/8209 , C12N15/8213 , C12N15/8242
摘要： A vector for introducing a desired gene into a planet, which comprises the desired gene and at least one morphological abnormally induction (MAI) gene as a marker gene, or which comprises the desired gene, at least one MAI gene and a removable element. A method for producing a transgenic plant free from the influence of a marker gene. A method for multitudinously introducing desired genes into one plant.
摘要翻译：用于将期望的基因引入行星的载体，其包含所需基因和至少一种形态异常诱导（MAI）基因作为标记基因，或包含所需基因，至少一种MAI基因和可移除元件。一种不受标记基因影响的转基因植物的制备方法。将所需基因大量引入一种植物的方法。

9. 发明授权

US09353379B2 Method for cultivation of genetically-modified plant 有权
标题翻译：转基因植物栽培方法
公开(公告)号：US09353379B2
公开(公告)日：2016-05-31
申请号：US13147436
申请日：2009-08-26
申请人： Saori Kasahara , Masafumi Wasai , Koichi Sugita , Teruhisa Shimada
发明人： Saori Kasahara , Masafumi Wasai , Koichi Sugita , Teruhisa Shimada
IPC分类号： A01H1/00 , C12N15/82 , A01H5/00 , A01H5/10 , C12N15/00
CPC分类号： C12N15/8261 , C12N15/8234 , C12N15/8238 , C12N15/8251 , C12N15/8257 , Y02A40/146
摘要： The present invention provides a method for cultivation of a genetically-modified plant that can highly produce a desired protein. More specifically, the present invention provides a method for cultivation of a genetically-modified plant comprising: cultivating the genetically-modified plant in a medium, wherein the genetically-modified plant is transformed by introducing an expression vector comprising a promoter regulating expression of RNA and a seed storage protein isolated from a plant that highly expresses RNA and the seed storage protein under a high nitrogen condition; and a polynucleotide encoding an objective protein, and wherein the medium is adjusted so that nitrate nitrogen is 70 mg/L to 750 mg/L and/or ammonium nitrogen is 70 mg/L to 750 mg/L for a definite period in a period from 30 days before an expected flowering date to a date on or before flowering of the genetically-modified plant.
摘要翻译：本发明提供了能够高度产生所需蛋白质的遗传修饰植物的培养方法。更具体地说，本发明提供了一种遗传修饰植物的培养方法，包括：在培养基中培养经遗传修饰的植物，其中转基因修饰植物通过引入包含调节RNA表达的启动子的表达载体和在高氮条件下从高度表达RNA和种子储存蛋白的植物中分离的种子储存蛋白; 和编码目标蛋白质的多核苷酸，并且调节培养基使得硝酸盐氮在70mg / L至750mg / L和/或铵氮为70mg / L至750mg / L的条件下在一定期间内从预计开花日期前30天到转基因植物开花前后的日期。

10. 发明授权

US07294761B2 Method for efficiently producing transgenic plant using auxin precursor 失效
标题翻译：使用生长素前体有效生产转基因植物的方法
公开(公告)号：US07294761B2
公开(公告)日：2007-11-13
申请号：US10626609
申请日：2003-07-25
申请人： Etsuko Matsunaga , Koichi Sugita , Hiroyasu Ebinuma
发明人： Etsuko Matsunaga , Koichi Sugita , Hiroyasu Ebinuma
IPC分类号： C12N15/82 , C12N15/83 , C12N15/84 , C12N15/31 , C12N15/54 , C12N15/55
CPC分类号： C12N15/821
摘要： A method for producing a transgenic plant, which comprises: (A) introducing a vector into a plant cell, wherein the vector is a vector for gene introduction into a plant and comprises: a desired gene, and a selectable marker gene comprising a gene encoding an enzyme which synthesizes auxin from an auxin precursor; (B) culturing the plant cell into which the genes are introduced by the vector, in the presence of an auxin precursor and/or an analogue thereof to thereby prepare a redifferentiated tissue, and detecting and selecting the redifferentiated tissues; and (C) culturing the redifferentiated tissue selected in (B) to redifferentiate a plant individual, and a vector for gene introduction into a plant, which comprises: a desired gene, and a selectable marker gene comprising an indoleacetamide hydrolase, iaaH, gene and an isopentenyl transferase, ipt, gene and being free of an tryptophan monooxygenase, iaaM, gene.
摘要翻译：一种生产转基因植物的方法，其包括：（A）将载体导入植物细胞，其中所述载体是用于将基因导入植物的载体，并且包含：所需基因和选择标记基因，所述选择标记基因包含编码从生长素前体合成生长素的酶; （B）在生长素前体和/或其类似物的存在下培养载体引入基因的植物细胞，由此制备再分化组织，并检测并选择再分化组织; （C）培养在（B）中选择的再分化组织以重新分化植物个体和将基因导入植物的载体，其包含：所需基因和包含吲哚乙酰胺水解酶，iaaH基因和异戊烯基转移酶，ipt，基因，并且不含色氨酸单加氧酶，iaaM基因。

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