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1. 发明授权

US07385758B2 Total internal reflection fluorescence microscope 有权
标题翻译：全内反射荧光显微镜
公开(公告)号：US07385758B2
公开(公告)日：2008-06-10
申请号：US10816489
申请日：2004-03-31
申请人： Yasushi Aono , Tsuyoshi Mochizuki , Kazuhiko Osa
发明人： Yasushi Aono , Tsuyoshi Mochizuki , Kazuhiko Osa
IPC分类号： G02B21/06
CPC分类号： G01N21/6458 , G01N21/648 , G02B21/16
摘要： A condenser lens is disposed in a position facing an objective lens via a specimen, a reflection mirror is movably disposed in the vicinity of an outermost part of a transmitted illuminative light path on the side of a transmitted illuminative light source from the condenser lens, and a laser beam output from a laser oscillation unit is reflected by the reflection mirror and is incident upon the condenser lens.
摘要翻译：聚光透镜通过试样设置在面向物镜的位置，反射镜可移动地设置在透镜照明光路的从聚光透镜侧透射的照明光源侧的最外部附近，以及从激光振荡单元输出的激光束被反射镜反射并入射到聚光透镜。

2. 发明授权

US06297904B1 Inverted confocal microscope 失效
标题翻译：倒置共聚焦显微镜
公开(公告)号：US06297904B1
公开(公告)日：2001-10-02
申请号：US09400410
申请日：1999-09-21
申请人： Hisao Kitagawa , Yasushi Aono , Kazuhiko Osa , Yosuke Kishi , Yoshihiro Shimada
发明人： Hisao Kitagawa , Yasushi Aono , Kazuhiko Osa , Yosuke Kishi , Yoshihiro Shimada
IPC分类号： G02B2100
CPC分类号： G02B21/0052 , G02B21/0048 , G02B21/0076 , G02B21/0088
摘要： An inverted microscope is provided that reflects an observation light passing through an image-formation optical system upward using at least one reflection optical system and that leads the observation light to an observation path. The inverted microscope includes a confocal scanner arranged at an image surface position of the image-formation optical system, a light source for applying light onto a sample through the confocal scanner and the image-formation optical system, and a confocal image formation optical system that leads the light passing through the confocal scanner to the sample through the image-formation optical system, returns a return light from the sample to the confocal scanner along a route opposite to that for leading the light to the sample, and obtains a confocal image. A confocal observation optical system is also provided for leading the return light from the sample passing through the optical scanner to the observation optical path.
摘要翻译：提供一种倒置显微镜，其使用至少一个反射光学系统向上反射通过图像形成光学系统的观察光，并将观察光引导到观察路径。倒置显微镜包括布置在图像形成光学系统的图像表面位置处的共焦扫描器，用于通过共焦扫描器和图像形成光学系统将光施加到样本上的光源，以及共焦图像形成光学系统，其中将通过共聚焦扫描器的光引导到通过图像形成光学系统的样品，沿着与将光导向样品的路线相反的路线从样品返回到共聚焦扫描器，并获得共聚焦图像。还提供了共焦观察光学系统，用于将来自通过光学扫描器的样品的返回光引导到观察光路。

3. 发明授权

US07245426B2 Total internal reflection illumination apparatus and microscope using this total internal reflection illumination apparatus 有权
标题翻译：全内反射照明装置和使用该全内反射照明装置的显微镜
公开(公告)号：US07245426B2
公开(公告)日：2007-07-17
申请号：US11432811
申请日：2006-05-11
申请人： Yasushi Aono , Tsuyoshi Mochizuki
发明人： Yasushi Aono , Tsuyoshi Mochizuki
IPC分类号： G02B21/06
CPC分类号： G02B21/16 , G02B21/06
摘要： A total internal reflection illumination apparatus applied to a microscope which illuminates a sample through an objective having a numerical aperture enabling total internal reflection illumination, comprises a first total internal reflection mirror which is arranged in the vicinity of an outermost peripheral part of an observation optical path of the microscope to reflect an incident illumination light in a direction of the objective, a second total internal reflection mirror which is arranged at a symmetrical position with the first total internal reflection mirror to sandwich an observation optical axis and reflects return light reflected on a surface of the sample in a direction different from the illumination optical path, and a return light dimming part configured to dim the return light reflected by the second total internal reflection mirror.
摘要翻译：应用于通过具有能够进行全内反射照明的数值孔径的物镜照射样品的显微镜的全内反射照明装置包括布置在观察光路的最外周部附近的第一全内反射镜的第二全反射镜，其被布置在与第一全内反射镜对称的位置处以夹持观察光轴并且反射在表面上反射的返回光在与所述照明光路不同的方向上的所述样本的反射光调光部，以及对由所述第二全内反射镜反射的返回光进行调光的返回光调光部。

4. 发明申请

US20060203331A1 Total internal reflection illumination apparatus and microscope using this total internal reflection illumination apparatus 有权
公开(公告)号：US20060203331A1
公开(公告)日：2006-09-14
申请号：US11432811
申请日：2006-05-11
申请人： Yasushi Aono , Tsuyoshi Mochizuki
发明人： Yasushi Aono , Tsuyoshi Mochizuki
IPC分类号： G02B21/06
CPC分类号： G02B21/16 , G02B21/06
摘要： A total internal reflection illumination apparatus applied to a microscope which illuminates a sample through an objective having a numerical aperture enabling total internal reflection illumination, comprises a first total internal reflection mirror which is arranged in the vicinity of an outermost peripheral part of an observation optical path of the microscope to reflect an incident illumination light in a direction of the objective, a second total internal reflection mirror which is arranged at a symmetrical position with the first total internal reflection mirror to sandwich an observation optical axis and reflects return light reflected on a surface of the sample in a direction different from the illumination optical path, and a return light dimming part configured to dim the return light reflected by the second total internal reflection mirror.

5. 发明授权

US06819484B2 Total internal reflection illumination apparatus and microscope using this total internal reflection illumination apparatus 有权
标题翻译：全内反射照明装置和使用该全内反射照明装置的显微镜
公开(公告)号：US06819484B2
公开(公告)日：2004-11-16
申请号：US10286639
申请日：2002-11-01
申请人： Yasushi Aono , Tsuyoshi Mochizuki
发明人： Yasushi Aono , Tsuyoshi Mochizuki
IPC分类号： G02B2100
CPC分类号： G02B21/16 , G02B21/06
摘要： A total internal reflection illumination apparatus applied to a microscope which illuminates a sample through an objective having a numerical aperture enabling total internal reflection illumination, comprises a first total internal reflection mirror which is arranged in the vicinity of an outermost peripheral part of an observation optical path of the microscope to reflect an incident illumination light in a direction of the objective, a second total internal reflection mirror which is arranged at a symmetrical position with the first total internal reflection mirror to sandwich an observation optical axis and reflects return light reflected on a surface of the sample in a direction different from the illumination optical path, and a return light dimming part configured to dim the return light reflected by the second total internal reflection mirror.
摘要翻译：应用于通过具有能够进行全内反射照明的数值孔径的物镜照射样品的显微镜的全内反射照明装置包括布置在观察光路的最外周部附近的第一全内反射镜的第二全反射镜，其被布置在与第一全内反射镜对称的位置处以夹持观察光轴并且反射在表面上反射的返回光在与所述照明光路不同的方向上的所述样本的反射光调光部，以及对由所述第二全内反射镜反射的返回光进行调光的返回光调光部。

6. 发明授权

US07315413B2 Illumination apparatus for microscope 有权
标题翻译：显微镜照明装置
公开(公告)号：US07315413B2
公开(公告)日：2008-01-01
申请号：US11269408
申请日：2005-11-08
申请人： Atsushi Miyawaki , Takashi Fukano , Yasushi Aono
发明人： Atsushi Miyawaki , Takashi Fukano , Yasushi Aono
IPC分类号： G02B21/06 , G02B21/00
CPC分类号： G02B27/144 , G02B21/16 , G02B27/1006 , G02B27/145
摘要： An illumination apparatus for a microscope includes a light source portion that projects a light beam, two splitting elements that split the light beam into three, wavelength selection elements that independently select transmission wavelengths of the three light beams, shutters that independently shield or guide the three light beams, a first combining element that combines optical paths of two light beams, a second combining element that combines an optical path of the remaining light beam with a combined optical path, a pinhole that is located on an optical path between the first and second combining elements and has an aperture that selectively transmits only part of a light beam, and a projection optical system that applies a light beam from the second combining element to a sample and, when applying a light beam from the pinhole to the sample, projects the aperture of the pinhole onto the sample.
摘要翻译：用于显微镜的照明装置包括投影光束的光源部分，将光束分成三个的分离元件，独立地选择三个光束的透射波长的波长选择元件，独立地屏蔽或引导三个光束的百叶窗光束，组合两个光束的光路的第一组合元件，将剩余光束的光路与组合光路组合的第二组合元件，位于第一和第二光束之间的光路上的针孔组合元件并且具有仅选择性地仅透射光束的一部分的孔径;以及投影光学系统，其将来自第二组合元件的光束施加到样本，并且当将来自针孔的光束施加到样品时，针孔的孔径到样品上。

7. 再颁专利

USRE38847E1 Reflected fluorescence microscope with multiple laser and excitation light sources 有权
标题翻译：反射荧光显微镜与多个激光和激发光源
公开(公告)号：USRE38847E1
公开(公告)日：2005-10-25
申请号：US10170874
申请日：2002-06-13
申请人： Yukio Nonoda , Yasushi Aono , Katsuyuki Abe
发明人： Yukio Nonoda , Yasushi Aono , Katsuyuki Abe
IPC分类号： G02B21/00 , G02B21/06 , G02B21/16 , G02B21/32
CPC分类号： G02B21/16 , G02B21/0088 , G02B21/32 , G02B2207/113
摘要： Caged reagent release experiments are carried out by introducing laser light of a laser light source for the Caged reagent release of a first reflected illumination optical system light path into a sample side through a first dichroic mirror on a cube turret and also introducing excitation light of a mercury lamp for the excitation light irradiation of a third reflected illumination optical system light path into the sample side through a first dichroic mirror on a slider. Laser trap experiments are carried out by introducing excitation light of a mercury lamp for the excitation light irradiation of a second reflected illumination optical system light path into the sample side through a second dichroic mirror on the cube turrent and also introducing laser light from the laser light source for the laser trap of the third reflected illumination optical system light path into the sample side through a second dichroic mirror on the slider.
摘要翻译：笼式试剂释放实验是通过将通过第一分色镜将第一反射照明光学系统光路的笼式试剂释放到样品侧中的激光源的激光引入立方体转台上的第一分色镜，并且还引入水银灯用于通过滑块上的第一分色镜将激发光照射到第三反射照明光学系统光路进入样品侧。激光阱实验是通过在立方体轮上引入第二反射照明光学系统光路的激发光照射到样品侧，并且还引入激光的激光来引入汞灯的激发光用于通过滑块上的第二分色镜将第三反射照明光学系统的激光阱的光源送入样品侧。

8. 发明申请

US20050231798A1 System microscope 有权
标题翻译：系统显微镜
公开(公告)号：US20050231798A1
公开(公告)日：2005-10-20
申请号：US11104844
申请日：2005-04-12
申请人： Susumu Honda , Yasushi Aono
发明人： Susumu Honda , Yasushi Aono
IPC分类号： G02B21/18 , G02B21/00 , G02B21/06
CPC分类号： G02B21/0088
摘要： A system microscope has a specimen mounting part which mounts a specimen, an inverted microscope including an illumination path which radiates illumination light from under the specimen mounting part, and an observation optical path which acquires detection light emitted from the specimen to which the illumination light is radiated, a microscope with upright frame including an illumination path which radiates illumination light from above the specimen mounting part, and an observation optical path which acquires detection light emitted from the specimen to which the illumination light is radiated, and a driving unit which moves at least one of the inverted microscope and the microscope with upright frame in a plane perpendicular to the observation optical path of the inverted microscope or the microscope with upright frame.
摘要翻译：系统显微镜具有安装试件的试样安装部，具有从试样安装部下方照射照明光的照明路径的倒置显微镜，以及取得从照明光所在的检体发出的检测光的观察光路具有直立框架的显微镜，包括从样本安装部分上方照射照明光的照明路径，以及取得从照射光照射的被检体发射的检测光的观察光路;以及驱动单元，倒置显微镜和显微镜中的至少一个具有直立框架的平面垂直于倒置显微镜的观察光路的平面或具有直立框架的显微镜。

9. 发明申请

US20070183030A1 TOTAL REFLECTION FLUORESCENT MICROSCOPE 审中-公开
标题翻译：总反射荧光显微镜
公开(公告)号：US20070183030A1
公开(公告)日：2007-08-09
申请号：US11734208
申请日：2007-04-11
申请人： Atsuhiro TSUCHIYA , Kenichi Kusaka , Yasushi Aono
发明人： Atsuhiro TSUCHIYA , Kenichi Kusaka , Yasushi Aono
IPC分类号： G02B21/06
CPC分类号： G02B21/0088 , G02B21/10 , G02B21/16
摘要： A fluorescent microscope comprises a light source, an optical illumination system which forms an optical path to irradiate a specimen with a light beam from the light source, an objective lens which condenses the light beam of the optical illumination system onto the specimen, an optical device which is disposed on the optical path of the optical illumination system and which decenters the light beam by decentering an optical axis of the optical path, and a slit which passes the light beam decentered by the optical device through a total reflection illumination region on an emission pupil surface of the objective lens.
摘要翻译：荧光显微镜包括光源，光学照明系统，其形成用于从来自光源的光束照射样本的光路;将光学照明系统的光束会聚到样本上的物镜，光学装置其布置在光学照明系统的光路上，并且通过使光路的光轴偏心而使光束偏心;以及狭缝，其通过光学装置偏振的光束通过发射的全反射照明区域物镜的瞳孔表面。

10. 发明申请

US20050002092A1 Total internal reflection illumination apparatus and microscope using this total internal reflection illumination apparatus 审中-公开
标题翻译：全内反射照明装置和使用该全内反射照明装置的显微镜
公开(公告)号：US20050002092A1
公开(公告)日：2005-01-06
申请号：US10902967
申请日：2004-07-29
申请人： Yasushi Aono , Tsuyoshi Hochizuki
发明人： Yasushi Aono , Tsuyoshi Hochizuki
IPC分类号： G02B21/06 , G02B21/00
CPC分类号： G02B21/16 , G02B21/06
摘要： A total internal reflection illumination apparatus applied to a microscope which illuminates a sample through an objective having a numerical aperture enabling total internal reflection illumination, comprises a first total internal reflection mirror which is arranged in the vicinity of an outermost peripheral part of an observation optical path of the microscope to reflect an incident illumination light in a direction of the objective, a second total internal reflection mirror which is arranged at a symmetrical position with the first total internal reflection mirror to sandwich an observation optical axis and reflects return light reflected on a surface of the sample in a direction different from the illumination optical path, and a return light dimming part configured to dim the return light reflected by the second total internal reflection mirror.
摘要翻译：应用于通过具有能够进行全内反射照明的数值孔径的物镜照射样品的显微镜的全内反射照明装置包括布置在观察光路的最外周部附近的第一全内反射镜的第二全反射镜，其被布置在与第一全内反射镜对称的位置处以夹持观察光轴并且反射在表面上反射的返回光在与所述照明光路不同的方向上的所述样本的反射光调光部，以及对由所述第二全内反射镜反射的返回光进行调光的返回光调光部。

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