专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5876942A Process for sexing cow embryos 失效
标题翻译：性交奶牛胚胎的过程
公开(公告)号：US5876942A
公开(公告)日：1999-03-02
申请号：US899811
申请日：1997-07-24
申请人： Winston Teng-Kuei Cheng , Chuan-Mu Chen , Che-Lin Hu , Chih-Hua Wang , Kong-Bung Choo
发明人： Winston Teng-Kuei Cheng , Chuan-Mu Chen , Che-Lin Hu , Chih-Hua Wang , Kong-Bung Choo
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6879
摘要： A rapid, highly reproducible and sensitive technique has been successfully developed for sexing the cow embryos, by method of polymerase chain reaction (PCR) against the amelogenin (bAML) genes located on both X- and Y-chromosomes of the Holstein dairy cattle. Results from DNA sequence analysis showed that there was only 45% homology between the intron 5 of AMLX and AMLY genes. Based on these sequences a pair of sex-specific primers, pbAML5XY(+) and pbAML5XY (-),were designed allowing to amplify a single fragment of 476-bp from the female cattle and two fragments of 476-bp and 341-bp from the male ones, respectively. The most important feature is that the precise sensitivity of sex-determination was confirmed to be reached as minimum template as trace amount of genomic DNA content in either a single lymphocyte or a single blastomere isolated from cow embryo at day-6 to day-7. Moreover, neither those of complicated procedures for purifying the DNA prior the PCR nor any extra pair of primers for serving as internal control is thought to be essential and the sex-determination of over hundred embryos can be completed at once within 4hrs.
摘要翻译：通过聚合酶链反应（PCR）对位于荷斯坦奶牛的X射线和Y染色体上的牙釉蛋白（bAML）基因的方法，已经成功开发了快速，高度重现和敏感的技术，用于性别雌性胚胎。 DNA序列分析结果表明，AMLX的内含子5与AMLY基因之间只有45％的同源性。基于这些序列，设计了一对性别特异性引物pbAML5XY（+）和pbAML5XY（ - ），从而扩增了来自雌性牛的476-bp的单个片段和476bp和341bp的两个片段男性分别。最重要的特征是确定了在第6天至第7天从单纯淋巴细胞或从胚胎中分离的单个卵裂球中的最小模板作为最小模板作为痕量的基因组DNA含量。此外，在PCR前纯化DNA的复杂程序和用作内部对照的任何额外的引物对都不被认为是至关重要的，并且可以在4小时内立即完成100多个胚胎的性别测定。

2. 发明申请

US20100125918A1 ENTEROVIRUS TYPE 71 PROTEIN AND METHOD FOR PRODUCTION 有权
标题翻译： EnterOVIRUS型71蛋白质和生产方法
公开(公告)号：US20100125918A1
公开(公告)日：2010-05-20
申请号：US12274194
申请日：2008-11-19
申请人： Chuan-Mu Chen , Hsiao-Ling Chen
发明人： Chuan-Mu Chen , Hsiao-Ling Chen
IPC分类号： A01K67/027 , C12P21/00 , C07K14/00 , C12N15/11 , C12N15/00 , A61K38/16
CPC分类号： C07K14/005 , A01K67/0275 , A01K2217/052 , A01K2217/206 , A01K2227/105 , A01K2267/01 , A01K2267/0337 , A61K38/00 , A61K39/00 , A61K2039/542 , C07K2319/02 , C12N15/8509 , C12N2770/32322
摘要： The invention provides a new type of a capsid protein VP1 of human enterovirus 71, named as MEL701-VP1 and functional/structural variants thereof, which is used for protection against enterovirus. The transgenic animal producing the protein, the composition comprising the protein and the method for production thereof are also provided.
摘要翻译：本发明提供了一种新型的人肠毒素71的衣壳蛋白VP1，命名为MEL701-VP1及其功能/结构变体，其用于保护免于肠道病毒。还提供了产生蛋白质的转基因动物，包含蛋白质的组合物及其生产方法。

3. 发明申请

US20050114911A1 Method for expressing multiple recombinant proteins in milk of transgenic non-human mammals 有权
标题翻译：用于在转基因非人哺乳动物的乳中表达多种重组蛋白的方法
公开(公告)号：US20050114911A1
公开(公告)日：2005-05-26
申请号：US10790720
申请日：2004-03-03
申请人： Shinn-Chih Wu , Teng-Kuei Cheng , Chuan-Mu Chen , Shau-Ping Lin , Chon-Ho Yen , Ping-Cheng Yang
发明人： Shinn-Chih Wu , Teng-Kuei Cheng , Chuan-Mu Chen , Shau-Ping Lin , Chon-Ho Yen , Ping-Cheng Yang
IPC分类号： A01K67/027
CPC分类号： A01K67/0275 , A01K2217/05 , A01K2227/108 , A01K2267/01
摘要： The present invention discloses a method for expressing multiple recombinant proteins in transgenic non-human mammalian milk, characterized in which human clotting factor IX gene and porcine lactoferrin gene are transferred into the mammal by gene injection and embryonic implantation to obtain expression in the milk of transgenic mammal and its filial generation. The method of this invention can maintain the stable expression of multiple recombinant proteins in the transgenic mammal during lactation and stable expression amount proximate to that of the first generation in the offsprings of the transgenic non-human mammal.
摘要翻译：本发明公开了一种在转基因非人哺乳动物乳中表达多种重组蛋白的方法，其特征在于通过基因注射和胚胎移植将人凝血因子IX基因和猪乳铁蛋白基因转移到哺乳动物中，以获得转基因乳中的表达哺乳动物及其子代。本发明的方法可以在哺乳期间保持转基因哺乳动物中多种重组蛋白的稳定表达，并且在转基因非人哺乳动物的后代中可以保持接近于第一代的稳定表达量。

4. 发明申请

US20140120173A1 Novel milk-fermented product and use thereof 审中-公开
标题翻译：新型乳发酵产品及其用途
公开(公告)号：US20140120173A1
公开(公告)日：2014-05-01
申请号：US13661080
申请日：2012-10-26
申请人： Chuan-Mu Chen , Hsiao-Ling Chen
发明人： Chuan-Mu Chen , Hsiao-Ling Chen
IPC分类号： C07K2/00 , A23C9/12 , A61K35/20
CPC分类号： C07K2/00 , A23C9/127 , A61K35/20 , A61K38/018
摘要： The present invention discloses a novel milk-fermented product comprising a group of peptides. The peptides are composed from no. 1 to no. 7 of the figure that is selected from the group consisting of FIG. 11 to FIG. 18. And the novel milk-fermented product is used for suppressing the reduction of bone mineral density. Therefore, the novel milk-fermented product could be a component of foods, nutrient supplement or medicine for treating or preventing osteoporosis.
摘要翻译：本发明公开了一种包含一组肽的新型乳发酵产品。肽由no组成。 1到否该图中的图7选自图1所示的组。图11〜而新型乳发酵产品用于抑制骨矿物质密度的降低。因此，新型乳发酵产品可以作为食品，营养补充剂或治疗或预防骨质疏松症的药物的成分。

5. 发明授权

US07667089B2 Transgenic mammal secreting B-domain deleted human FVII in its milk 有权
标题翻译：转基因哺乳动物在其牛奶中分泌B结构域缺失的人FVII
公开(公告)号：US07667089B2
公开(公告)日：2010-02-23
申请号：US10820777
申请日：2004-04-09
申请人： Winston T. K. Cheng , Chuan-Mu Chen , Shwu-Wha Lin , Chih-Hong Wang , Chih-Jen Lin , Shinn-Chih Wu
发明人： Winston T. K. Cheng , Chuan-Mu Chen , Shwu-Wha Lin , Chih-Hong Wang , Chih-Jen Lin , Shinn-Chih Wu
IPC分类号： C12P21/00 , A01K67/027 , C12N15/00
CPC分类号： C12N15/8509 , A01K67/0275 , A01K2217/05 , A01K2267/01 , C07K14/755 , C12N2830/008
摘要： Hemophilia A is one of the major inherited bleeding disorders caused by a deficiency or abnormality in coagulation factor VIII (FVIII). Hemophiliacs have been treated with whole plasma or purified FVIII concentrates. The risk of transmitting blood-borne viruses and the cost of highly purified FVIII are the major factors that restrict prophylaxis in hemophilia therapy. One of the challenges created by the biotechnology revolution is the development of methods for the economical production of highly purified proteins in large scales. The present invention provides improved mammary expression cassettes useful for the expression of genes at high levels in the milk of transgenic animals. In particular, the present invention provides recombinant signal peptide sequences derived from a-lactalbumin and aS1-casein milk genes suitable for leading protein secretion in the mammary gland. These gene cassettes are capable of delivering different transgenic constructs which result in the production of full-length or B domain-deleted therapeutic levels of biologically active human FVIII in the transgenic animals in vivo. Within the scope of the invention are also method for producing the transgenic non-human mammal, such as mouse, rat, rabbit, goat, sheep, pig and bovine species, capable of expressing human FVIII, and methods of making milk and methods of identifying protein from the transgenic milk.
摘要翻译：血友病A是凝血因子VIII（FVIII）缺乏或异常引起的主要遗传性出血性疾病之一。已经用全血浆或纯化的FVIII浓缩物治疗血友病患者。传播血源性病毒的风险和高度纯化的FVIII的成本是限制血友病治疗预防的主要因素。生物技术革命带来的挑战之一是开发大规模经济生产高纯度蛋白质的方法。本发明提供了可用于在转基因动物的牛奶中高水平表达基因的改进的乳腺表达盒。特别地，本发明提供了衍生自α-乳白蛋白的重组信号肽序列和适合于在乳腺中引导蛋白质分泌的α1酪蛋白乳基因。这些基因盒能够递送不同的转基因构建体，其导致在转基因动物体内产生全长或B结构域缺失的生物活性人类FVIII的治疗水平。在本发明的范围内，也是用于生产能够表达人FVIII的转基因非人哺乳动物如小鼠，大鼠，兔，山羊，绵羊，猪和牛物种的方法，以及制备牛奶的方法和鉴定方法来自转基因乳的蛋白质。

6. 发明授权

US08313750B2 Enterovirus type 71 protein and method for production 有权
公开(公告)号：US08313750B2
公开(公告)日：2012-11-20
申请号：US13034355
申请日：2011-02-24
申请人： Chuan-Mu Chen , Hsiao-Ling Chen
发明人： Chuan-Mu Chen , Hsiao-Ling Chen
IPC分类号： A61K39/12 , C07H21/02 , C07H21/04
CPC分类号： C07K14/005 , A01K67/0275 , A01K2217/052 , A01K2217/206 , A01K2227/105 , A01K2267/01 , A01K2267/0337 , A61K38/00 , A61K39/00 , A61K2039/542 , C07K2319/02 , C12N15/8509 , C12N2770/32322
摘要： The invention provides a new type of a capsid protein VP1 of human enterovirus 71, named as MEL701-VP1 and functional/structural variants thereof, which is used for protection against enterovirus. The transgenic animal producing the protein, the composition comprising the protein and the method for production thereof are also provided.

7. 发明授权

US08168192B2 Enterovirus type 71 protein and method for production 有权
标题翻译：肠病毒71型蛋白及其生产方法
公开(公告)号：US08168192B2
公开(公告)日：2012-05-01
申请号：US12274194
申请日：2008-11-19
申请人： Chuan-Mu Chen , Hsiao-Ling Chen
发明人： Chuan-Mu Chen , Hsiao-Ling Chen
IPC分类号： A61K39/12 , A61K39/00 , A61K39/38
CPC分类号： C07K14/005 , A01K67/0275 , A01K2217/052 , A01K2217/206 , A01K2227/105 , A01K2267/01 , A01K2267/0337 , A61K38/00 , A61K39/00 , A61K2039/542 , C07K2319/02 , C12N15/8509 , C12N2770/32322
摘要： The invention provides a new type of a capsid protein VP1 of human enterovirus 71, named as MEL701-VP1 and functional/structural variants thereof, which is used for protection against enterovirus. The transgenic animal producing the protein, the composition comprising the protein and the method for production thereof are also provided.
摘要翻译：本发明提供了一种新型的人肠毒素71的衣壳蛋白VP1，命名为MEL701-VP1及其功能/结构变体，其用于保护免于肠道病毒。还提供了产生蛋白质的转基因动物，包含蛋白质的组合物及其生产方法。

8. 发明申请

US20050229261A1 Method for producing biologically active human factor VIII in the milk of transgenic animals driven by mammary-specific expression cassettes 有权
标题翻译：在乳腺特异性表达盒驱动的转基因动物的乳中生产生物活性人因子VIII的方法
公开(公告)号：US20050229261A1
公开(公告)日：2005-10-13
申请号：US10820777
申请日：2004-04-09
申请人： Winston Cheng , Chuan-Mu Chen , Shwu-Wha Lin , Chih-Hong Wang , Chih-Jen Lin , Shinn-Chih Wu
发明人： Winston Cheng , Chuan-Mu Chen , Shwu-Wha Lin , Chih-Hong Wang , Chih-Jen Lin , Shinn-Chih Wu
IPC分类号： A01K67/027 , C07K14/755 , C12N15/85
CPC分类号： C12N15/8509 , A01K67/0275 , A01K2217/05 , A01K2267/01 , C07K14/755 , C12N2830/008
摘要： Hemophilia A is one of the major inherited bleeding disorders caused by a deficiency or abnormality in coagulation factor VIII (FVIII). Hemophiliacs have been treated with whole plasma or purified FVIII concentrates. The risk of transmitting blood-borne viruses and the cost of highly purified FVIII are the major factors that restrict prophylaxis in hemophilia therapy. One of the challenges created by the biotechnology revolution is the development of methods for the economical production of highly purified proteins in large scales. The present invention provides improved mammary expression cassettes useful for the expression of genes at high levels in the milk of transgenic animals. In particular, the present invention provides recombinant signal peptide sequences derived from a-lactalbumin and aS1-casein milk genes suitable for leading protein secretion in the mammary gland. These gene cassettes are capable of delivering different transgenic constructs which result in the production of full-length or B domain-deleted therapeutic levels of biologically active human FVIII in the transgenic animals in vivo. Within the scope of the invention are also method for producing the transgenic non-human mammal, such as mouse, rat, rabbit, goat, sheep, pig and bovine species, capable of expressing human FVIII, and methods of making milk and methods of identifying protein from the transgenic milk.
摘要翻译：血友病A是凝血因子VIII（FVIII）缺乏或异常引起的主要遗传性出血性疾病之一。已经用全血浆或纯化的FVIII浓缩物治疗血友病患者。传播血源性病毒的风险和高度纯化的FVIII的成本是限制血友病治疗预防的主要因素。生物技术革命带来的挑战之一是开发大规模经济生产高纯度蛋白质的方法。本发明提供了可用于在转基因动物的牛奶中高水平表达基因的改进的乳腺表达盒。特别地，本发明提供了衍生自α-乳白蛋白的重组信号肽序列和适合于在乳腺中引导蛋白质分泌的α1酪蛋白乳基因。这些基因盒能够递送不同的转基因构建体，其导致在转基因动物体内产生全长或B结构域缺失的生物活性人类FVIII的治疗水平。在本发明的范围内，也是用于生产能够表达人FVIII的转基因非人哺乳动物如小鼠，大鼠，兔，山羊，绵羊，猪和牛物种的方法，以及制备牛奶的方法和鉴定方法来自转基因乳的蛋白质。

9. 发明授权

US08247644B2 Method for manufacturing animal model for researching pulmonary tumor and use thereof 有权
标题翻译：制造肺肿瘤研究动物模型的方法及其应用
公开(公告)号：US08247644B2
公开(公告)日：2012-08-21
申请号：US12828786
申请日：2010-07-01
申请人： Chuan-Mu Chen
发明人： Chuan-Mu Chen
IPC分类号： A01K67/033 , A01K67/00 , C12N15/00
CPC分类号： A01K67/0275 , A01K2217/052 , A01K2227/105 , A01K2267/03 , A01K2267/0325
摘要： The present invention is a method for manufacturing an animal model for researching a pulmonary tumor and a use thereof. A transgenic non-human animal of the present invention is prepared by embryonic gene microinjection and possesses a tissue-specific expression of vascular endothelial growth factor A165 (VEGF-A165) in lung. Through the expression of vascular endothelial growth factor A165, the lung cells in the transgenic non-human animal of the present invention have inflammatory, vascularogenesis and angiogenesis responses or induce lung tumors. Thus, the non-human animal of the present invention can serve as an animal model for analyzing the regulation and the anti-tumor drugs screening of pulmonary adenocarcinoma.
摘要翻译：本发明是一种用于制造用于研究肺肿瘤的动物模型及其用途的方法。通过胚胎基因显微注射制备本发明的转基因非人动物，并且在肺中具有血管内皮生长因子A165（VEGF-A165）的组织特异性表达。通过血管内皮生长因子A165的表达，本发明的转基因非人动物中的肺细胞具有炎性，血管发生和血管生成反应或诱导肺肿瘤。因此，本发明的非人动物可以作为用于分析肺腺癌的调节和抗肿瘤药物筛选的动物模型。

10. 发明授权

US07763281B2 Antihypertensive peptide and use thereof 有权
标题翻译：抗高血压肽及其用途
公开(公告)号：US07763281B2
公开(公告)日：2010-07-27
申请号：US11856262
申请日：2007-09-17
申请人： Hsiao-Ling Chen , Chuan-Mu Chen
发明人： Hsiao-Ling Chen , Chuan-Mu Chen
IPC分类号： A61K35/20 , A61K38/00 , A01N37/18 , C07K5/00
CPC分类号： C07K14/4732 , A23C9/127 , A23L33/18 , A61K38/00
摘要： The present invention provides a novel antihypertensive peptide. Also provided are pharmaceutical and food compositions containing the antihypertensive peptide for treating hypertension or cardiovascular disease.
摘要翻译：本发明提供了一种新的抗高血压肽。还提供含有用于治疗高血压或心血管疾病的抗高血压肽的药物和食品组合物。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式