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1. 发明授权

US07795234B2 Newly identified human small intestine extracellular lactase-phlorizin hydrolase (ecLPH) gene 有权
标题翻译：新鉴定的人类小肠细胞外乳糖酶 - 根皮苷水解酶（ecLPH）基因
公开(公告)号：US07795234B2
公开(公告)日：2010-09-14
申请号：US11901989
申请日：2007-09-20
申请人： Chuan-Mu Chen , Winston T. K. Cheng , Hsiao-Ling Chen
发明人： Chuan-Mu Chen , Winston T. K. Cheng , Hsiao-Ling Chen
IPC分类号： A01N43/04 , C07H21/04 , C12N15/00
CPC分类号： C12N9/2405 , A01K67/0275 , A01K2217/05 , A01K2227/101 , A01K2227/102 , A01K2227/103 , A01K2227/105 , A01K2267/02 , C07K2319/02 , C12N15/8509 , C12N2830/008 , C12Y302/01108
摘要： The invention provides a transgenic animal producing low-lactose milk, which is transformed with a gene encoding an extracellular lactase-hydrolyzing enzyme cloned from a human small intestinal cDNA library. The invention also provides a new extracellular lactase-phlorizin hydrolase (ecLPH) gene that can express human lactase-hydrolyzing enzyme in the mammary gland of animals. The invention can be used in the production of low-lactose milk.
摘要翻译：本发明提供一种产生低乳糖的转基因动物，其用编码从人小肠cDNA文库克隆的细胞外乳糖酶水解酶的基因转化。本发明还提供了可以在动物乳腺中表达人乳糖酶水解酶的新的细胞外乳糖酶 - 根皮苷水解酶（ecLPH）基因。本发明可用于生产低乳糖乳。

2. 发明授权

US07667089B2 Transgenic mammal secreting B-domain deleted human FVII in its milk 有权
标题翻译：转基因哺乳动物在其牛奶中分泌B结构域缺失的人FVII
公开(公告)号：US07667089B2
公开(公告)日：2010-02-23
申请号：US10820777
申请日：2004-04-09
申请人： Winston T. K. Cheng , Chuan-Mu Chen , Shwu-Wha Lin , Chih-Hong Wang , Chih-Jen Lin , Shinn-Chih Wu
发明人： Winston T. K. Cheng , Chuan-Mu Chen , Shwu-Wha Lin , Chih-Hong Wang , Chih-Jen Lin , Shinn-Chih Wu
IPC分类号： C12P21/00 , A01K67/027 , C12N15/00
CPC分类号： C12N15/8509 , A01K67/0275 , A01K2217/05 , A01K2267/01 , C07K14/755 , C12N2830/008
摘要： Hemophilia A is one of the major inherited bleeding disorders caused by a deficiency or abnormality in coagulation factor VIII (FVIII). Hemophiliacs have been treated with whole plasma or purified FVIII concentrates. The risk of transmitting blood-borne viruses and the cost of highly purified FVIII are the major factors that restrict prophylaxis in hemophilia therapy. One of the challenges created by the biotechnology revolution is the development of methods for the economical production of highly purified proteins in large scales. The present invention provides improved mammary expression cassettes useful for the expression of genes at high levels in the milk of transgenic animals. In particular, the present invention provides recombinant signal peptide sequences derived from a-lactalbumin and aS1-casein milk genes suitable for leading protein secretion in the mammary gland. These gene cassettes are capable of delivering different transgenic constructs which result in the production of full-length or B domain-deleted therapeutic levels of biologically active human FVIII in the transgenic animals in vivo. Within the scope of the invention are also method for producing the transgenic non-human mammal, such as mouse, rat, rabbit, goat, sheep, pig and bovine species, capable of expressing human FVIII, and methods of making milk and methods of identifying protein from the transgenic milk.
摘要翻译：血友病A是凝血因子VIII（FVIII）缺乏或异常引起的主要遗传性出血性疾病之一。已经用全血浆或纯化的FVIII浓缩物治疗血友病患者。传播血源性病毒的风险和高度纯化的FVIII的成本是限制血友病治疗预防的主要因素。生物技术革命带来的挑战之一是开发大规模经济生产高纯度蛋白质的方法。本发明提供了可用于在转基因动物的牛奶中高水平表达基因的改进的乳腺表达盒。特别地，本发明提供了衍生自α-乳白蛋白的重组信号肽序列和适合于在乳腺中引导蛋白质分泌的α1酪蛋白乳基因。这些基因盒能够递送不同的转基因构建体，其导致在转基因动物体内产生全长或B结构域缺失的生物活性人类FVIII的治疗水平。在本发明的范围内，也是用于生产能够表达人FVIII的转基因非人哺乳动物如小鼠，大鼠，兔，山羊，绵羊，猪和牛物种的方法，以及制备牛奶的方法和鉴定方法来自转基因乳的蛋白质。

3. 发明授权

US07501554B2 Transgenic animals producing low-lactose milk and newly identified human small intestinal extracellular lactase-phlorizin hydrolase (ecLPH) gene 有权
标题翻译：产生低乳糖乳和新鉴定的人小肠细胞外乳糖酶 - 根皮苷水解酶（ecLPH）基因的转基因动物
公开(公告)号：US07501554B2
公开(公告)日：2009-03-10
申请号：US11434332
申请日：2006-05-15
申请人： Chuan-Mu Chen , Winston T. K. Cheng , Hsiao-Ling Chen
发明人： Chuan-Mu Chen , Winston T. K. Cheng , Hsiao-Ling Chen
IPC分类号： A01K67/027 , C12N15/00
CPC分类号： C12N9/2405 , A01K67/0275 , A01K2217/05 , A01K2227/101 , A01K2227/102 , A01K2227/103 , A01K2227/105 , A01K2267/02 , C07K2319/02 , C12N15/8509 , C12N2830/008 , C12Y302/01108
摘要： The invention provides a transgenic animal producing low-lactose milk, which is transformed with a gene encoding an extracellular lactase-hydrolyzing enzyme cloned from a human small intestinal cDNA library. The invention also provides a new extracellular lactase-phlorizin hydrolase (ecLPH) gene that can express human lactase-hydrolyzing enzyme in the mammary gland of animals. The invention can be used in the production of low-lactose milk.
摘要翻译：本发明提供一种产生低乳糖的转基因动物，其用编码从人小肠cDNA文库克隆的细胞外乳糖酶水解酶的基因转化。本发明还提供了可以在动物乳腺中表达人乳糖酶水解酶的新的细胞外乳糖酶 - 根皮苷水解酶（ecLPH）基因。本发明可用于生产低乳糖乳。

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