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    • 4. 发明申请
    • METHOD FOR DETERMINING EQUIVALENT THERMAL CONDITIONS BETWEEN LIQUID CHROMATOGRAPHY SYSTEMS
    • 用于确定液相色谱系统之间等效热条件的方法
    • WO2011091224A1
    • 2011-07-28
    • PCT/US2011/021999
    • 2011-01-21
    • WATERS TECHNOLOGIES CORPORATIONANDREWS, Richard, W.BEALS, Peyton, C.
    • ANDREWS, Richard, W.BEALS, Peyton, C.
    • B01D15/10
    • G01N30/04B01D15/424G01N30/8672G01N2030/047G01N2030/3007
    • Described is a method of transferring a chromatographic method between liquid chromatography (LC) systems. The method is based on a determination of an isoretention temperature at which two solutes co-elute. The method enables separations to be performed using different LC systems with reproducible and equivalent results. For example, the method allows for a chromatography method developed for HPLC to be more readily transferred to a UPLC system and for a chromatography method developed for a UPLC system to be more readily transferred to a HPLC system. The method addresses LC systems having column ovens of different design in which the internal column temperatures are not equal although the operating temperatures of the column ovens may be accurately controlled to equal values. The retention behavior and resolution of different LC systems is caused to be substantially the same so that equivalent separation results are obtained.
    • 描述了在液相色谱(LC)系统之间转移色谱方法的方法。 该方法基于两个溶质共洗脱的等离子体停留温度的测定。 该方法可以使用具有可重现性和等效结果的不同液相色谱系统进行分离。 例如,该方法允许为HPLC开发的色谱方法更容易转移到UPLC系统中,并且为UPLC系统开发的色谱方法更容易转移到HPLC系统。 该方法解决了具有不同设计的列炉的LC系统,其中内部柱温度不相等,尽管柱式炉的操作温度可以被精确地控制为相等的值。 导致不同LC系统的保持行为和分辨率基本相同,从而获得等效的分离结果。