专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO03003014A9 METHODS AND COMPOSITIONS FOR DETERMINING THE PURITY OF AND PURIFYING CHEMICALLY SYNTHESIZED NUCLEIC ACIDS 审中-公开
标题翻译：用于测定和纯化化学合成核酸的方法和组合物
公开(公告)号：WO03003014A9
公开(公告)日：2004-05-13
申请号：PCT/US0220418
申请日：2002-06-28
申请人： VERI Q INC , PROTEOME SCIENCES PLC , PEARCE CHRISTOPHER D J , MITCHELL LLOYD G
发明人： PEARCE CHRISTOPHER D J , MITCHELL LLOYD G
IPC分类号： C07K16/44 , C12M1/00 , C12M1/34 , C12N15/09 , C12P19/34 , C12Q1/68 , C40B40/06 , C40B50/14 , G01N33/53 , G01N33/543 , G01N33/566 , G01N33/58 , G01N37/00 , C07H21/00 , C07H21/02 , C07H21/04
CPC分类号： B82Y30/00 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00675 , B01J2219/00693 , B01J2219/00722 , C07K16/44 , C12P19/34 , C12Q1/6804 , C12Q1/6837 , C40B40/06 , C40B50/14 , G01N33/5308 , G01N33/543 , G01N2500/00 , Y02P20/55 , C12Q2565/531 , C12Q2563/131
摘要： An antibody microarray is described comprising a plurality of antibodies immobilized on a substrate, wherein each antibody specifically binds to a synthetic oligomer (e.g., an oligonucleotide or oligopeptide) having an organic protecting group covalently bound thereto, which antibody does not bind to that synthetic oligomer when the organic protecting group is not covalently bound thereto. Methods of making and using such antibodies are disclosed, along with cells for making such antibodies. Methods of making and using such antibody microarrays are also disclosed.
摘要翻译：描述了抗体微阵列，其包含固定在底物上的多种抗体，其中每种抗体特异性结合具有与其共价结合的有机保护基的合成低聚物（例如，寡核苷酸或寡肽），该抗体不与该合成寡聚物结合当有机保护基团不共价结合时。公开了制备和使用这些抗体的方法以及用于制备这种抗体的细胞。还公开了制备和使用这种抗体微阵列的方法。

2. 发明专利

JP2009162769A Methods and compositions for determining purity of chemically synthesized nucleic acid and purifying chemically synthesized nucleic acid 有权
标题翻译：用于测定化学合成核酸的纯度和纯化合成核酸的方法和组合物
公开(公告)号：JP2009162769A
公开(公告)日：2009-07-23
申请号：JP2009048133
申请日：2009-03-02
申请人： Proteome Sciences Plc , Veri-Q Inc , ヴェリ‐キュー,インコーポレイテッド , プロテオム・サイエンシズ・パブリック・リミテッド・カンパニー
发明人： PEARCE CHRISTOPHER D J , MITCHELL LLOYD G
IPC分类号： G01N33/53 , C07K16/44 , C12M1/00 , C12M1/34 , C12N15/09 , C12P19/34 , C12Q1/68 , C40B40/06 , C40B50/14 , G01N33/543 , G01N33/566 , G01N33/58 , G01N37/00
CPC分类号： B82Y30/00 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00675 , B01J2219/00693 , B01J2219/00722 , C07K16/44 , C12P19/34 , C12Q1/6804 , C12Q1/6837 , C40B40/06 , C40B50/14 , G01N33/5308 , G01N33/543 , G01N2500/00 , Y02P20/55 , C12Q2565/531 , C12Q2563/131
摘要： PROBLEM TO BE SOLVED: To provide a means for detecting, identifying, and quantifying the grade of deprotection of the synthesized product of nucleic acid sequence, and to provide a means for purifying chemically synthesized nucleic acid. SOLUTION: An antibody microarray is described comprising a plurality of antibodies immobilized on a substrate, wherein each antibody specifically binds to a synthetic oligomer (e.g. an oligonucleotide or oligopeptide) having an organic protecting group covalently bound thereto, which antibody does not bind to that synthetic oligomer when the organic protecting group is not covalently bound thereto. Methods of making and using such antibodies are disclosed, along with cells for making such antibodies. Methods of making and using such antibody microarrays are also disclosed. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供用于检测，鉴定和定量核酸序列的合成产物的脱保护等级的手段，并提供用于纯化化学合成的核酸的手段。描述了包含固定在底物上的多种抗体的抗体微阵列，其中每种抗体特异性结合具有共价结合的有机保护基的合成低聚物（例如寡核苷酸或寡肽），该抗体不结合当有机保护基团不与其共价键合时与该合成低聚物反应。公开了制备和使用这些抗体的方法以及用于制备这种抗体的细胞。还公开了制备和使用这种抗体微阵列的方法。版权所有（C）2009，JPO＆INPIT

3. 发明申请

WO0149745A9 METHODS AND COMPOSITIONS FOR DETERMINING THE PURITY OF CHEMICALLY SYNTHESIZED NUCLEIC ACIDS 审中-公开
标题翻译：用于测定化学合成核酸的纯度的方法和组合物
公开(公告)号：WO0149745A9
公开(公告)日：2001-09-20
申请号：PCT/US0035600
申请日：2000-12-29
申请人： UNIV NORTH CAROLINA STATE , INTRONN , AGRIS PAUL F , MITCHELL LLOYD G , PEARCE CHRISTOPHER D J
发明人： AGRIS PAUL F , MITCHELL LLOYD G , PEARCE CHRISTOPHER D J
IPC分类号： G01N33/53 , B01J20/281 , C07K16/18 , C07K16/44 , C12M1/00 , C12N5/10 , C12N15/09 , C12P21/08 , C12Q1/68 , G01N30/88 , G01N37/00 , G01N33/531 , G01N33/552 , G01N33/577
CPC分类号： C07K16/44 , B01J2219/00274 , C07K16/18 , C12Q1/6804 , Y02P20/55
摘要： This application describes an antibody that specifically binds to a synthetic oligomer (e.g., an oligonucleotide or oligopeptide) having a organic protecting group covalently bound thereto, which antibody does not bind to that synthetic oligomer when the organic protecting group is not covalently bound thereto. Methods of making and using such antibodies are also disclosed, along with cells for making such antibodies and articles carrying immobilized oligomers that can be used in assay procedures with such antibodies.
摘要翻译：本申请描述了与有机保护基团共价结合的合成寡聚物（例如寡核苷酸或寡肽）特异性结合的抗体，当有机保护基团不与其共价键合时，该抗体不与该合成低聚物结合。还公开了制备和使用这些抗体的方法，以及用于制备此类抗体的细胞和携带固定化寡聚物的制品，其可用于具有此类抗体的测定程序。

4. 发明专利

CA2452690A1 METHODS AND COMPOSITIONS FOR DETERMINING THE PURITY OF AND PURIFYING CHEMICALLY SYNTHESIZED NUCLEIC ACIDS 未知
公开(公告)号：CA2452690A1
公开(公告)日：2003-01-09
申请号：CA2452690
申请日：2002-06-28
申请人： VERI Q INC , PROTEOME SCIENCES PLC
发明人： PEARCE CHRISTOPHER D J , MITCHELL LLOYD G
IPC分类号： C07K16/44 , C12M1/00 , C12M1/34 , C12N15/09 , C12P19/34 , C12Q1/68 , C12Q1/6804 , C12Q1/6837 , C40B40/06 , C40B50/14 , G01N33/53 , G01N33/543 , G01N33/566 , G01N33/58 , G01N37/00 , C07K17/00 , C07H21/00 , C12N15/10
摘要： An antibody microarray is described comprising a plurality of antibodies immobilized on a substrate, wherein each antibody specifically binds to a synthetic oligomer (e.g., an oligonucleotide or oligopeptide) having an organic protecting group covalently bound thereto, which antibody does not bind to that synthetic oligomer when the organic protecting group is not covalently bound thereto. Methods of making and using such antibodies are disclosed, along with cells for making such antibodies. Methods of making an d using such antibody microarrays are also disclosed.

5. 发明公开

EP1412752A4 METHODS AND COMPOSITIONS FOR DETERMINING THE PURITY OF AND PURIFYING CHEMICALLY SYNTHESIZED NUCLEIC ACIDS 有权
标题翻译： VERFAHREN UND ZUSAMMENSETZUNGEN ZUR REINHEITSBESTIMMUNG UND REINIGUNG CHEMISCH SYNTHETIERERTERNUKLEINSÄUREN
公开(公告)号：EP1412752A4
公开(公告)日：2004-09-29
申请号：EP02747993
申请日：2002-06-28
申请人： VERI Q INC , PROTEOME SCIENCES PLC
发明人： PEARCE CHRISTOPHER D J , MITCHELL LLOYD G
IPC分类号： C07K16/44 , C12M1/00 , C12M1/34 , C12N15/09 , C12P19/34 , C12Q1/68 , C40B40/06 , C40B50/14 , G01N33/53 , G01N33/543 , G01N33/566 , G01N33/58 , G01N37/00 , C07H21/00 , C07H21/02 , C07H21/04
CPC分类号： B82Y30/00 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00675 , B01J2219/00693 , B01J2219/00722 , C07K16/44 , C12P19/34 , C12Q1/6804 , C12Q1/6837 , C40B40/06 , C40B50/14 , G01N33/5308 , G01N33/543 , G01N2500/00 , Y02P20/55 , C12Q2565/531 , C12Q2563/131
摘要： An antibody microarray is described comprising a plurality of antibodies immobilized on a substrate, wherein each antibody specifically binds to a synthetic oligomer (e.g., an oligonucleotide or oligopeptide) having an organic protecting group covalently bound thereto, which antibody does not bind to that synthetic oligomer when the organic protecting group is not covalently bound thereto. Methods of making and using such antibodies are disclosed, along with cells for making such antibodies. Methods of making and using such antibody microarrays are also disclosed.
摘要翻译：描述了包含固定在底物上的多种抗体的抗体微阵列，其中每种抗体特异性结合具有与其共价结合的有机保护基团的合成寡聚体（例如，寡核苷酸或寡肽），该抗体不结合该合成寡聚体当有机保护基团不与其共价结合时。公开了制备和使用这些抗体的方法，以及用于制备这种抗体的细胞。还公开了制造和使用这种抗体微阵列的方法。

6. 发明专利

CA2452690C METHODS AND COMPOSITIONS FOR DETERMINING THE PURITY OF AND PURIFYING CHEMICALLY SYNTHESIZED NUCLEIC ACIDS 未知
公开(公告)号：CA2452690C
公开(公告)日：2016-08-16
申请号：CA2452690
申请日：2002-06-28
申请人： VERI-Q INC , PROTEOME SCIENCES PLC
发明人： PEARCE CHRISTOPHER D J , MITCHELL LLOYD G
IPC分类号： C40B50/14 , C07H21/00 , C07K16/44 , C07K17/00 , C12M1/00 , C12M1/34 , C12N15/09 , C12N15/10 , C12P19/34 , C12Q1/68 , C12Q1/6804 , C12Q1/6837 , C40B30/04 , C40B40/06 , C40B40/10 , G01N33/53 , G01N33/543 , G01N33/566 , G01N33/58 , G01N37/00
摘要： An antibody microarray is described comprising a plurality of antibodies immobilized on a substrate, wherein each antibody specifically binds to a synthetic oligomer (e.g., an oligonucleotide or oligopeptide) having an organic protecting group covalently bound thereto, which antibody does not bind to that synthetic oligomer when the organic protecting group is not covalently bound thereto. Methods of making and using such antibodies are disclosed, along with cells for making such antibodies. Methods of making and using such antibody microarrays are also disclosed.

7. 发明专利

AU2002318434B2 Methods and compositions for determining the purity of and purifying chemically synthesized nucleic acids 未知
公开(公告)号：AU2002318434B2
公开(公告)日：2008-05-15
申请号：AU2002318434
申请日：2002-06-28
申请人： PROTEOME SCIENCES PLC , VERI Q INC
发明人： PEARCE CHRISTOPHER D J , MITCHELL LLOYD G
IPC分类号： G01N33/543 , C07K16/44 , C12M1/00 , C12M1/34 , C12N15/09 , C12P19/34 , C12Q1/68 , C12Q1/6804 , C12Q1/6837 , C40B40/06 , C40B50/14 , G01N33/53 , G01N33/566 , G01N33/58 , G01N37/00
摘要： An antibody microarray is described comprising a plurality of antibodies immobilized on a substrate, wherein each antibody specifically binds to a synthetic oligomer (e.g., an oligonucleotide or oligopeptide) having an organic protecting group covalently bound thereto, which antibody does not bind to that synthetic oligomer when the organic protecting group is not covalently bound thereto. Methods of making and using such antibodies are disclosed, along with cells for making such antibodies. Methods of making and using such antibody microarrays are also disclosed.

8. 发明专利

ES2401121T3 Dispositivos de recolección de muestras y métodos que utilizan marcadores y el uso de dichos marcadores como controles en la validación de una muestra, evaluación y/o certificación de laboratorio 未知
公开(公告)号：ES2401121T3
公开(公告)日：2013-04-17
申请号：ES97909878
申请日：1997-10-03
申请人： INTRONN LLC , VERI Q INC
发明人： MITCHELL LLOYD G
IPC分类号： B01L3/00 , G01N33/48 , C12N15/09 , C12Q1/02 , C12Q1/68 , C12Q1/6876 , G01N1/00 , G01N1/28 , G01N1/38 , G01N33/487 , G01N35/00
摘要： Un dispositivo apropiado para marcar una muestra recogida, y dicho dispositivo comprende:un medio de recogida que comprende un tubo o un sistema de recogida de membrana para recoger la muestra, enel que el medio de recogida incluye un revestimiento que comprende al menos un marcador detectable en unasuperficie interior del mismo; y en el que al menos un marcador detectable puede entrar en contacto con la muestraen el momento de recogida de la muestra para marcar la muestra recogida tras el contacto de la muestra con lasuperficie interior del medio de recogida, la cual tiene al menos un marcador detectable contenido en la misma; y enel que al menos un marcador detectable es distinto al componente que ya está presente en la muestra y es inerte acualquier componente presente en la muestra; y en el que al menos una parte del marcador detectable pasa a lamuestra recogida tras la recogida de la muestra para marcar dicha muestra, y que se caracteriza porque el marcadordetectable es un marcador que no es de ácido nucleico.

9. 发明申请

WO2006026611A3 REVERSE TRANSCRIPTASE MEDIATED RNA GENE EXPRESSION 审中-公开
标题翻译：逆转录酶介导的RNA基因表达
公开(公告)号：WO2006026611A3
公开(公告)日：2009-04-16
申请号：PCT/US2005030825
申请日：2005-08-30
申请人： MITCHELL LLOYD G
发明人： MITCHELL LLOYD G
IPC分类号： C12Q1/00 , C12Q1/68 , C12Q1/70
CPC分类号： C12N9/1276
摘要： The present invention provides methods and compositions for expressing a polypeptide or nucleic acid of interest in cells expressing reverse transcriptase (RT). The compositions of the invention include reverse transcriptase RNA (RTD-RNA) molecules that are designed to be reverse transcribed into a DNA molecule capable of encoding a polypeptide or nucleic acid of interest. Polypeptides of interest include cytotoxic, therapeutic, enzymatic, or reporter polypeptides. Nucleic acid molecules of interest include those capable of regulating, altering or reprogramming gene expression, for example, those capable of functioning as antisense, aptamer, decoy, inhibitor, ribozyme, or RNAi molecules.
摘要翻译：本发明提供用于在表达逆转录酶（RT）的细胞中表达感兴趣的多肽或核酸的方法和组合物。本发明的组合物包括逆转录酶RNA（RTD-RNA）分子，其被设计为逆转录成能够编码感兴趣的多肽或核酸的DNA分子。感兴趣的多肽包括细胞毒性，治疗性，酶学或报道多肽。感兴趣的核酸分子包括能够调节，改变或重编程基因表达的核酸分子，例如能够作为反义，适体，诱饵，抑制剂，核酶或RNAi分子起作用的核酸分子。

10. 发明申请

WO2004038380A2 SCREENING METHODS FOR IDENTIFICATION OF EFFICIENT PRE-TRANS-SPLICING MOLECULES 审中-公开
标题翻译：用于识别有效预分离分子的筛选方法
公开(公告)号：WO2004038380A2
公开(公告)日：2004-05-06
申请号：PCT/US0334102
申请日：2003-10-23
申请人： INTRONN INC , MITCHELL LLOYD G , PUTTARAJU MADAIAH , GARCIA-BLANCO MARIANO , OTTO EDWARD , YANG YANPING
发明人： MITCHELL LLOYD G , PUTTARAJU MADAIAH , GARCIA-BLANCO MARIANO , OTTO EDWARD , YANG YANPING
IPC分类号： C07H21/04 , C12Q1/68 , G01N20060101 , G01N33/50 , G01N33/53 , G01N
CPC分类号： C12N15/1027 , C12N15/1086 , G01N33/5023
摘要： The present invention provides methods and compositions for rapid high capacity functional screening to identify optimal pre-trans-splicing molecules (PTMs). The compositions of the invention include PTM expression libraries capable of encoding candidate PTMs designed to interact with a target precursor messenger RNA molecule (target pre-mRNA) and mediate a trans-splicing reaction resulting in the generation of a novel chimeric RNA molecule (chimeric RNA). The candidate PTMs of the invention encode a portion of a first reporter molecule and may encode one or more other reporter molecules, which can be used to select for cells expressing optimal PTMs (efficient and specific). The compositions of the invention also include cells that express a target pre-mRNA encoding the remaining portion of the first reporter molecule. The screening methods of the invention encompass (i) contacting a PTM expression library with cells expressing a target pre-mRNA under conditions in which a trans-splicing reaction will occur in the presence of an optimal PTM (expressed by the library vector) resulting in the formation of a chimeric repaired RNA molecule capable of encoding at least one reporter molecule; (ii) selecting for cells expressing the repaired reporter molecule wherein expression of the reporter molecule indicates the presence of an optimal PTM in the selected cell; and (iii) identifying the optimal PTM expressed in the selected cell(s). The additional reporter molecule(s) can be used to assess both specific and non-specific trans-splicing, as well direct PTM expression.
摘要翻译：本发明提供用于快速高容量功能筛选以鉴定最佳预转录分子（PTM）的方法和组合物。本发明的组合物包括能够编码设计成与靶前体信使RNA分子（靶前体mRNA）相互作用的候选PTM的PTM表达文库，并介导产生新型嵌合RNA分子的转拼反应（嵌合RNA ）。本发明的候选PTM编码第一报告分子的一部分并且可以编码一个或多个其它报告分子，其可用于选择表达最佳PTM（有效和特异性）的细胞。本发明的组合物还包括表达编码第一报告分子的剩余部分的靶mRNA的细胞。本发明的筛选方法包括（i）在最优PTM（由文库载体表达）存在下在其中将发生转拼反应的条件下使PTM表达文库与表达靶mRNA的细胞接触，导致形成能够编码至少一个报告分子的嵌合修复的RNA分子; （ii）选择表达修复的报告分子的细胞，其中报道分子的表达指示所选细胞中存在最佳PTM; 和（iii）鉴定在所选择的细胞中表达的最佳PTM。另外的报告分子可用于评估特异性和非特异性转拼，以及直接PTM表达。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式