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1. 发明申请

WO2004038380A2 SCREENING METHODS FOR IDENTIFICATION OF EFFICIENT PRE-TRANS-SPLICING MOLECULES 审中-公开
标题翻译：用于识别有效预分离分子的筛选方法
公开(公告)号：WO2004038380A2
公开(公告)日：2004-05-06
申请号：PCT/US0334102
申请日：2003-10-23
申请人： INTRONN INC , MITCHELL LLOYD G , PUTTARAJU MADAIAH , GARCIA-BLANCO MARIANO , OTTO EDWARD , YANG YANPING
发明人： MITCHELL LLOYD G , PUTTARAJU MADAIAH , GARCIA-BLANCO MARIANO , OTTO EDWARD , YANG YANPING
IPC分类号： C07H21/04 , C12Q1/68 , G01N20060101 , G01N33/50 , G01N33/53 , G01N
CPC分类号： C12N15/1027 , C12N15/1086 , G01N33/5023
摘要： The present invention provides methods and compositions for rapid high capacity functional screening to identify optimal pre-trans-splicing molecules (PTMs). The compositions of the invention include PTM expression libraries capable of encoding candidate PTMs designed to interact with a target precursor messenger RNA molecule (target pre-mRNA) and mediate a trans-splicing reaction resulting in the generation of a novel chimeric RNA molecule (chimeric RNA). The candidate PTMs of the invention encode a portion of a first reporter molecule and may encode one or more other reporter molecules, which can be used to select for cells expressing optimal PTMs (efficient and specific). The compositions of the invention also include cells that express a target pre-mRNA encoding the remaining portion of the first reporter molecule. The screening methods of the invention encompass (i) contacting a PTM expression library with cells expressing a target pre-mRNA under conditions in which a trans-splicing reaction will occur in the presence of an optimal PTM (expressed by the library vector) resulting in the formation of a chimeric repaired RNA molecule capable of encoding at least one reporter molecule; (ii) selecting for cells expressing the repaired reporter molecule wherein expression of the reporter molecule indicates the presence of an optimal PTM in the selected cell; and (iii) identifying the optimal PTM expressed in the selected cell(s). The additional reporter molecule(s) can be used to assess both specific and non-specific trans-splicing, as well direct PTM expression.
摘要翻译：本发明提供用于快速高容量功能筛选以鉴定最佳预转录分子（PTM）的方法和组合物。本发明的组合物包括能够编码设计成与靶前体信使RNA分子（靶前体mRNA）相互作用的候选PTM的PTM表达文库，并介导产生新型嵌合RNA分子的转拼反应（嵌合RNA ）。本发明的候选PTM编码第一报告分子的一部分并且可以编码一个或多个其它报告分子，其可用于选择表达最佳PTM（有效和特异性）的细胞。本发明的组合物还包括表达编码第一报告分子的剩余部分的靶mRNA的细胞。本发明的筛选方法包括（i）在最优PTM（由文库载体表达）存在下在其中将发生转拼反应的条件下使PTM表达文库与表达靶mRNA的细胞接触，导致形成能够编码至少一个报告分子的嵌合修复的RNA分子; （ii）选择表达修复的报告分子的细胞，其中报道分子的表达指示所选细胞中存在最佳PTM; 和（iii）鉴定在所选择的细胞中表达的最佳PTM。另外的报告分子可用于评估特异性和非特异性转拼，以及直接PTM表达。

2. 发明公开

EP2889658A4 SURFACE STRUCTURE FOR LIGHT GUIDE PLATE AND APPLICATION AND MANUFACTURING METHOD THEREFOR 审中-公开
标题翻译：表面结构的调光电路板及其用途及其制造方法
公开(公告)号：EP2889658A4
公开(公告)日：2016-07-20
申请号：EP12883153
申请日：2012-08-21
申请人： ZHANG DAHAI , YANG YANPING
发明人： YANG YANPING , ZHANG DAHAI
IPC分类号： G02B6/10 , B43L1/10 , G02F1/13357
CPC分类号： G02B6/0036 , B43L1/10 , G02B6/0035 , G02B6/0038 , G02F1/133615

3. 发明专利

AU2003285009A1 SCREENING METHODS FOR IDENTIFICATION OF EFFICIENT PRE-TRANS-SPLICING MOLECULES 未知
公开(公告)号：AU2003285009A1
公开(公告)日：2004-05-13
申请号：AU2003285009
申请日：2003-10-23
申请人： INTRONN INC
发明人： YANG YANPING , MITCHELL LLOYD G , PUTTARAJU MADAIAH , GARCIA-BLANCO MARIANO , OTTO EDWARD
IPC分类号： C07H21/04 , C12Q1/68 , G01N20060101 , G01N33/50 , G01N33/53
摘要： The present invention provides methods and compositions for rapid high capacity functional screening to identify optimal pre-trans-splicing molecules (PTMs). The compositions of the invention include PTM expression libraries capable of encoding candidate PTMs designed to interact with a target precursor messenger RNA molecule (target pre-mRNA) and mediate a trans-splicing reaction resulting in the generation of a novel chimeric RNA molecule (chimeric RNA). The candidate PTMs of the invention encode a portion of a first reporter molecule and may encode one or more other reporter molecules, which can be used to select for cells expressing optimal PTMs (efficient and specific). The compositions of the invention also include cells that express a target pre-mRNA encoding the remaining portion of the first reporter molecule. The screening methods of the invention encompass (i) contacting a PTM expression library with cells expressing a target pre-mRNA under conditions in which a trans-splicing reaction will occur in the presence of an optimal PTM (expressed by the library vector) resulting in the formation of a chimeric repaired RNA molecule capable of encoding at least one reporter molecule; (ii) selecting for cells expressing the repaired reporter molecule wherein expression of the reporter molecule indicates the presence of an optimal PTM in the selected cell; and (iii) identifying the optimal PTM expressed in the selected cell(s). The additional reporter molecule(s) can be used to assess both specific and non-specific trans-splicing, as well direct PTM expression.

4. 发明专利

CA2503247A1 SCREENING METHODS FOR IDENTIFICATION OF EFFICIENT PRE-TRANS-SPLICING MOLECULES 未知
公开(公告)号：CA2503247A1
公开(公告)日：2004-05-06
申请号：CA2503247
申请日：2003-10-23
申请人： INTRONN INC
发明人： MITCHELL LLOYD G , YANG YANPING , PUTTARAJU MADAIAH , GARCIA-BLANCO MARIANO , OTTO EDWARD
IPC分类号： C07H21/04 , C12Q1/68 , G01N20060101 , G01N33/50 , G01N33/53
摘要： The present invention provides methods and compositions for rapid high capacity functional screening to identify optimal pre-trans-splicing molecul es (PTMs). The compositions of the invention include PTM expression libraries capable of encoding candidate PTMs designed to interact with a target precursor messenger RNA molecule (target pre-mRNA) and mediate a trans- splicing reaction resulting in the generation of a novel chimeric RNA molecu le (chimeric RNA). The candidate PTMs of the invention encode a portion of a first reporter molecule and may encode one or more other reporter molecules, which can be used to select for cells expressing optimal PTMs (efficient and specific). The compositions of the invention also include cells that express a target pre-mRNA encoding the remaining portion of the first reporter molecul e. The screening methods of the invention encompass (i) contacting a PTM expression library with cells expressing a target pre-mRNA under conditions in which a trans-splicing reaction will occur in the presence of an optimal PTM (expressed by the library vector) resulting in the formation of a chimeric repaired RNA molecule capable of encoding at least one reporter molecule; (i i) selecting for cells expressing the repaired reporter molecule wherein expression of the reporter molecule indicates the presence of an optimal PTM in the selected cell; and (iii) identifying the optimal PTM expressed in the selected cell(s). The additional reporter molecule(s) can be used to assess both specific and non-specific trans-splicing, as well direct PTM expression .

5. 发明公开

EP1579004A4 SCREENING METHODS FOR IDENTIFICATION OF EFFICIENT PRE-TRANS-SPLICING MOLECULES 有权
标题翻译：筛选方法，确定有效的PRE-TRANS-SPLEISSMOLEKÜLE
公开(公告)号：EP1579004A4
公开(公告)日：2006-06-28
申请号：EP03779324
申请日：2003-10-23
申请人： INTRONN INC
发明人： MITCHELL LLOYD G , PUTTARAJU MADAIAH , GARCIA-BLANCO MARIANO , OTTO EDWARD , YANG YANPING
IPC分类号： C07H21/04 , C12Q1/68 , G01N20060101 , G01N33/50 , G01N33/53
CPC分类号： C12N15/1027 , C12N15/1086 , G01N33/5023
摘要： The present invention provides methods and compositions for rapid high capacity functional screening to identify optimal pre-trans-splicing molecules (PTMs). The compositions of the invention include PTM expression libraries capable of encoding candidate PTMs designed to interact with a target precursor messenger RNA molecule (target pre-mRNA) and mediate a trans-splicing reaction resulting in the generation of a novel chimeric RNA molecule (chimeric RNA). The candidate PTMs of the invention encode a portion of a first reporter molecule and may encode one or more other reporter molecules, which can be used to select for cells expressing optimal PTMs (efficient and specific). The compositions of the invention also include cells that express a target pre-mRNA encoding the remaining portion of the first reporter molecule. The screening methods of the invention encompass (i) contacting a PTM expression library with cells expressing a target pre-mRNA under conditions in which a trans-splicing reaction will occur in the presence of an optimal PTM (expressed by the library vector) resulting in the formation of a chimeric repaired RNA molecule capable of encoding at least one reporter molecule; (ii) selecting for cells expressing the repaired reporter molecule wherein expression of the reporter molecule indicates the presence of an optimal PTM in the selected cell; and (iii) identifying the optimal PTM expressed in the selected cell(s). The additional reporter molecule(s) can be used to assess both specific and non-specific trans-splicing, as well direct PTM expression.

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