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1. 发明授权

US5034330A Preparation of a selectively permeable membrane for an enzyme electrode 失效
标题翻译：制备用于酶电极的选择性渗透膜
公开(公告)号：US5034330A
公开(公告)日：1991-07-23
申请号：US301032
申请日：1989-01-24
申请人： Tsunefumi Yamori , Eiji Yuasa , Ryuzo Hayashi
发明人： Tsunefumi Yamori , Eiji Yuasa , Ryuzo Hayashi
IPC分类号： G01N27/327 , C12N11/08 , C12Q1/00 , G01N27/30 , G01N27/416
CPC分类号： C12Q1/002 , Y10S435/817
摘要： A selectively permeable membrane for an enzyme electrode is prepared by forming a mixture of an emulsion of resin containing at least one prepolymer or monomer having ethylenic unsaturation, and a water-soluble polymer material, and subjecting the mixture to irradiation with ionizing or ultraviolet irradiation. The membrane is permeable to substances produced by an enzyme reaction while preventing the permeation of higher molecular weight materials. An immobilized enzyme membrane is formed on an outer surface of the permeable membrane, and the resultant membrane is mounted on an electrically conductive base to form an enzyme electrode. In another embodiment, the selectively permeable membrane is formed on the electrically conductive base, and then the immobilized enzyme membrane is formed. The selectively permeable membrane is situated between the immobilized enzyme membrane, which is in contact with a sample solution, and the electrically conductive base.

2. 发明授权

US5306413A Assay apparatus and assay method 失效
标题翻译：测定仪器和测定方法
公开(公告)号：US5306413A
公开(公告)日：1994-04-26
申请号：US37421
申请日：1993-03-26
申请人： Ryuzo Hayashi , Yukie Inoue , Akio Kariyone
发明人： Ryuzo Hayashi , Yukie Inoue , Akio Kariyone
IPC分类号： C12Q1/00 , G01N27/26
CPC分类号： C12Q1/005 , Y10S435/817
摘要： The present invention relates to a simple and yet multifunctional system for assay employing immobilized enzymes.
摘要翻译：本发明涉及使用固定化酶的简单而又多功能的测定系统。

3. 发明授权

US08790156B2 Tooth-profile management system for shaving-cutter grinding machine 有权
标题翻译：剃须刀磨床牙齿管理系统
公开(公告)号：US08790156B2
公开(公告)日：2014-07-29
申请号：US13502752
申请日：2010-10-06
申请人： Yoshikoto Yanase , Ryuzo Hayashi
发明人： Yoshikoto Yanase , Ryuzo Hayashi
IPC分类号： B24B49/18
CPC分类号： B23F19/06 , B23F21/28 , B24B49/02 , B24B53/085
摘要： A personal computer has correction coefficients (α) for tooth-profile error correction and correction coefficients (β) for meshing position correction which are set for each number of times a shaving cutter is sharpened, and for each cutter feature of the shaving cutter. Target tooth-profile data (Do), tooth-profile error data (ΔD) which is the difference between the target tooth-profile data (Do) and measured tooth-profile data (Dm), and the correction coefficient (α) and the correction coefficient (β) captured in correspondence with the number of times sharpening is performed, and the cutter features are applied to an equation Dcc=Do+α·ΔD+β to find aimed tooth-profile data (Dcc). Shaving-cutter tooth-profile data (ds) is found from the aimed tooth-profile data (Dcc). Thus, the shaving cutter can be sharpened appropriately even when the outer diameter and tooth thickness of the shaving cutter are reduced by sharpening the shaving cutter.
摘要翻译：个人计算机具有用于齿廓修正的校正系数（α）和针对剃须刀被削尖的每个次数设定的啮合位置校正的校正系数（＆bgr），以及剃须刀的每个切割器特征。目标齿廓数据（Do），作为目标齿廓数据（Do）与测量齿廓数据（Dm）之间的差异的齿廓误差数据（＆Dgr; D）和校正系数（α）并且与执行锐化次数相对应地捕获的校正系数（＆bgr），并且切割器特征被应用于等式Dcc = Do +α·＆Dgr; D +＆bgr; 找到目标牙齿轮廓数据（Dcc）。从目标牙齿轮廓数据（Dcc）中可以看到剃刀齿廓数据（ds）。因此，即使通过磨削剃须刀来减少剃须刀的外径和齿厚度，也可以适当地削尖剃须刀。

4. 发明授权

US5320939A Measuring apparatus of two components using enzyme electrodes and the measuring method thereof 失效
标题翻译：使用酶电极的两个组分的测量装置及其测量方法
公开(公告)号：US5320939A
公开(公告)日：1994-06-14
申请号：US953294
申请日：1992-09-30
申请人： Yoshio Hashizume , Ryuzo Hayashi , Akio Kariyone
发明人： Yoshio Hashizume , Ryuzo Hayashi , Akio Kariyone
IPC分类号： G01N27/26 , C12Q1/00 , G01N27/27 , G01N27/403 , G01N27/416
CPC分类号： C12Q1/001 , Y10S435/817
摘要： In a two-substrate measuring apparatus employing a first enzyme electrode for responding only to a first substrate to be measured and a second enzyme electrode for responding to both first and second substrates to be measured, in which the same enzymatic reactions are used for forming detectable substances and the substances to be finally detected by the electrodes are identical, when calculating the calibration curve for the second substrate to be measured, the output portion due to traces of the first substrate contained in the second substrate to be measured is removed, so that the second substrate is measured at a high sensitivity and high accuracy.
摘要翻译：在使用第一酶电极仅响应待测量的第一基底的第二酶电极和用于响应于待测量的第一和第二基底的第二酶电极的双基底测量装置中，其中使用相同的酶反应形成可检测的物质和由电极最终检测到的物质在计算待测量的第二基板的校准曲线时是相同的，除去由于包含在待测量的第二基板中的第一基板的痕迹引起的输出部分，从而以高灵敏度和高精度测量第二基板。

5. 发明授权

US4971901A Process for preparing enzyme electrodes 失效
标题翻译：制备酶电极的方法
公开(公告)号：US4971901A
公开(公告)日：1990-11-20
申请号：US146202
申请日：1988-01-20
申请人： Ryuzo Hayashi , Akio Kariyone
发明人： Ryuzo Hayashi , Akio Kariyone
IPC分类号： G01N27/327 , C12Q1/00
CPC分类号： C12Q1/005 , Y10S435/817
摘要： An enzyme electrode is prepared by a process wherein an albumin solution containing 0.05 to 0.5% by weight of a multifunctional aldehyde is applied onto the surface of an electroconductive substrate while maintaining the atmospheric temperature at 0.degree. to 20.degree. C. to thereby form a crosslinked albumin layer on the surface of the electroconductive substrate. Thereafter a solution containing 0.5% by weight or less of a multifunctional aldehyde and a predetermined amount of an oxidase capable of producing hydrogen peroxide (H.sub.2 O.sub.2) is applied on the surface of the previously formed crosslinked albumin layer to form a H.sub.2 O.sub.2 productive oxidase containing layer.

6. 发明申请

US20120252318A1 TOOTH-PROFILE MANAGEMENT SYSTEM FOR SHAVING-CUTTER GRINDING MACHINE 有权
标题翻译：切割机研磨机的牙齿型材管理系统
公开(公告)号：US20120252318A1
公开(公告)日：2012-10-04
申请号：US13502752
申请日：2010-10-06
申请人： Yoshikoto Yanase , Ryuzo Hayashi
发明人： Yoshikoto Yanase , Ryuzo Hayashi
IPC分类号： B24B3/12
CPC分类号： B23F19/06 , B23F21/28 , B24B49/02 , B24B53/085
摘要： A personal computer has correction coefficients (α) for tooth-profile error correction and correction coefficients (β) for meshing position correction which are set for each number of times a shaving cutter is sharpened, and for each cutter feature of the shaving cutter. Target tooth-profile data (Do), tooth-profile error data (ΔD) which is the difference between the target tooth-profile data (Do) and measured tooth-profile data (Dm), and the correction coefficient (α) and the correction coefficient (β) captured in correspondence with the number of times sharpening is performed, and the cutter features are applied to an equation Dcc=Do+α·ΔD+β to find aimed tooth-profile data (Dcc). Shaving-cutter tooth-profile data (ds) is found from the aimed tooth-profile data (Dcc). Thus, the shaving cutter can be sharpened appropriately even when the outer diameter and tooth thickness of the shaving cutter are reduced by sharpening the shaving cutter.
摘要翻译：个人计算机具有用于齿廓修正的校正系数（α）和针对剃须刀被削尖的每个次数设定的啮合位置校正的校正系数（＆bgr），以及剃须刀的每个切割器特征。目标齿廓数据（Do），作为目标齿廓数据（Do）与测量齿廓数据（Dm）之间的差异的齿廓误差数据（＆Dgr; D）和校正系数（α）并且与执行锐化次数相对应地捕获的校正系数（＆bgr），并且切割器特征被应用于等式Dcc = Do +α·＆Dgr; D +＆bgr; 找到目标牙齿轮廓数据（Dcc）。从目标牙齿轮廓数据（Dcc）中可以看到剃刀齿廓数据（ds）。因此，即使通过磨削剃须刀来减少剃须刀的外径和齿厚度，也可以适当地削尖剃须刀。

7. 发明授权

US5248597A Method and apparatus for analyzing starch and related carbohydrates 失效
标题翻译：分析淀粉和相关碳水化合物的方法和装置
公开(公告)号：US5248597A
公开(公告)日：1993-09-28
申请号：US682953
申请日：1991-04-10
申请人： Ryuzo Hayashi , Yoshio Hasizume , Akio Kariyone
发明人： Ryuzo Hayashi , Yoshio Hasizume , Akio Kariyone
IPC分类号： C12M1/40 , C12Q1/00 , C12Q1/26 , C12Q1/40 , C12Q1/54 , G01N33/66
CPC分类号： G01N33/66 , C12Q1/005
摘要： A method and apparatus for the determination of the average molecular weight, average chain length or dextrose equivalent of starch and related carbohydrates containing glucose units. The starch is analyzed by determining the free glucose amount (Gf) contained in starch or related carbohydrate sample, determining a gross glucose amount (Gt) rendered by hydrolysis of starch or related carbohydrates, and further determining a glucose amount (Gr) rendered by hydrolysis of a reduced product which is obtained by reduction of reducing terminals contained in starch or related carbohydrate.The average molecular weight, average chain length or dextrose equivalent of the starch is determined using the formulas stated below from the measured amounts of the free glucose amount (Gf), and the glucose amount (Gr). The apparatus preferably utilized immobilized enzymes to conduct the hydrolysis of the starch sample, contains a device for recognizing and storing the measured values and computed data, and contains further a device for computing average molecular weight (Ma), average chain length (Dp) or dextrose equivalent value (DE) according to the following expressions: Dp=2.multidot.Gt/(Gt-Gr+Gf), Ma=18(19.multidot.Gt+Gf-Gr)/(Gt-Gr+Gf), DE=1000(Gt-Gr+Gf)/(19.multidot.Gt-Gr+Gf), or DE=1000/(9.multidot.Dp+1).

8. 发明授权

US5242793A Selective permeable membrane and electrode using the same 失效
标题翻译：选择性渗透膜和使用其的电极
公开(公告)号：US5242793A
公开(公告)日：1993-09-07
申请号：US490709
申请日：1990-03-07
申请人： Akio Kariyone , Ryuzo Hayashi , Yoshio Hashizume
发明人： Akio Kariyone , Ryuzo Hayashi , Yoshio Hashizume
IPC分类号： B01D69/14 , C12N11/02 , C12N11/10 , C12Q1/00 , G01N27/40
CPC分类号： C12Q1/003 , B01D69/141 , C12N11/02 , C12N11/10 , C12Q1/002 , G01N27/3271 , Y10S435/817
摘要： An electrode comprising an electrically conductive base, and a selectively permeable membrane which is produced by forming a membrane from a mixed solution comprising (a) albumin, (b) at least one type of crosslinking agent and (c) chitosan. The selectively permeable membrane is useful, e.g., in conjuction with an electrode. An enzyme electrode is also provided having the selectively permeable membrane disposed on the electrode and an immobilized enzyme membrane disposed on the selectively permeable membrane. The immobilized enzyme membrane is prepared by incorporating a carboxylate into the solution containing the enzyme and an aldehyde as the crosslinking agent. The crosslinking is effected by heating or drying.

9. 发明授权

US5081037A Enzyme electrode for measuring malto-oligosaccharide 失效
标题翻译：用于测量MALTO-OLIGOSACCHARIDE的酶电极
公开(公告)号：US5081037A
公开(公告)日：1992-01-14
申请号：US321165
申请日：1989-03-09
申请人： Akio Kariyone , Yoshio Hashizume , Ryuzo Hayashi
发明人： Akio Kariyone , Yoshio Hashizume , Ryuzo Hayashi
IPC分类号： G01N27/327 , C12M1/40 , C12Q1/00 , G01N27/30 , G01N27/416
CPC分类号： C12Q1/005 , Y10S435/817 , Y10S435/913 , Y10S435/939
摘要： An enzyme electrode for measuring malto-oligosaccharides having a co-immobilized enzyme membrane containing glucoamylase and a glucose oxidizing enzyme such as glucose oxidase or pyranose oxidase.The glucoamylase and glucose oxidizing enzyme are immobilized at a ratio of ##EQU1## where Va is the maximum reaction rate of glucoamylase as expressed in the glucose formation rate from maltose, and Vo is the maximum reaction rate of glucose oxidizing enzyme as expressed in the hydrogen peroxide formation rate from glucose.In such enzyme electrode, malto-oligosaccharides are detected at the response value proportional to their degree of polymerization. Therefore, the malto-oligosaccharides can be measured at the glucose converted concentration.

10. 发明授权

US08128802B2 Analysis apparatus and analysis method for glycosylated hemoglobin 有权
标题翻译：糖化血红蛋白的分析仪器及分析方法
公开(公告)号：US08128802B2
公开(公告)日：2012-03-06
申请号：US11913367
申请日：2006-05-02
申请人： Yoko Nanjo , Ryuzo Hayashi
发明人： Yoko Nanjo , Ryuzo Hayashi
IPC分类号： G01N27/327
CPC分类号： C12Q1/005 , G01N33/723
摘要： Disclosed is a method for calculating a ratio of glycosylated hemoglobin with high accuracy by electrochemically detecting the concentration of fructosyl valine or fructosyl valyl-histidine in a sample. Also disclosed is an apparatus for assaying glucose and glycosylated hemoglobin simultaneously. Further disclosed are a method and an apparatus for removing hydrogen peroxide in a sample.
摘要翻译：公开了通过电化学检测样品中果糖基缬氨酸或果糖基缬氨酰基 - 组氨酸的浓度来计算糖基化血红蛋白的比例高的方法。还公开了同时测定葡萄糖和糖基化血红蛋白的装置。还公开了一种用于去除样品中的过氧化氢的方法和装置。

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