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1. 发明授权

US5306413A Assay apparatus and assay method 失效
标题翻译：测定仪器和测定方法
公开(公告)号：US5306413A
公开(公告)日：1994-04-26
申请号：US37421
申请日：1993-03-26
申请人： Ryuzo Hayashi , Yukie Inoue , Akio Kariyone
发明人： Ryuzo Hayashi , Yukie Inoue , Akio Kariyone
IPC分类号： C12Q1/00 , G01N27/26
CPC分类号： C12Q1/005 , Y10S435/817
摘要： The present invention relates to a simple and yet multifunctional system for assay employing immobilized enzymes.
摘要翻译：本发明涉及使用固定化酶的简单而又多功能的测定系统。

2. 发明授权

US5320939A Measuring apparatus of two components using enzyme electrodes and the measuring method thereof 失效
标题翻译：使用酶电极的两个组分的测量装置及其测量方法
公开(公告)号：US5320939A
公开(公告)日：1994-06-14
申请号：US953294
申请日：1992-09-30
申请人： Yoshio Hashizume , Ryuzo Hayashi , Akio Kariyone
发明人： Yoshio Hashizume , Ryuzo Hayashi , Akio Kariyone
IPC分类号： G01N27/26 , C12Q1/00 , G01N27/27 , G01N27/403 , G01N27/416
CPC分类号： C12Q1/001 , Y10S435/817
摘要： In a two-substrate measuring apparatus employing a first enzyme electrode for responding only to a first substrate to be measured and a second enzyme electrode for responding to both first and second substrates to be measured, in which the same enzymatic reactions are used for forming detectable substances and the substances to be finally detected by the electrodes are identical, when calculating the calibration curve for the second substrate to be measured, the output portion due to traces of the first substrate contained in the second substrate to be measured is removed, so that the second substrate is measured at a high sensitivity and high accuracy.
摘要翻译：在使用第一酶电极仅响应待测量的第一基底的第二酶电极和用于响应于待测量的第一和第二基底的第二酶电极的双基底测量装置中，其中使用相同的酶反应形成可检测的物质和由电极最终检测到的物质在计算待测量的第二基板的校准曲线时是相同的，除去由于包含在待测量的第二基板中的第一基板的痕迹引起的输出部分，从而以高灵敏度和高精度测量第二基板。

3. 发明授权

US4971901A Process for preparing enzyme electrodes 失效
标题翻译：制备酶电极的方法
公开(公告)号：US4971901A
公开(公告)日：1990-11-20
申请号：US146202
申请日：1988-01-20
申请人： Ryuzo Hayashi , Akio Kariyone
发明人： Ryuzo Hayashi , Akio Kariyone
IPC分类号： G01N27/327 , C12Q1/00
CPC分类号： C12Q1/005 , Y10S435/817
摘要： An enzyme electrode is prepared by a process wherein an albumin solution containing 0.05 to 0.5% by weight of a multifunctional aldehyde is applied onto the surface of an electroconductive substrate while maintaining the atmospheric temperature at 0.degree. to 20.degree. C. to thereby form a crosslinked albumin layer on the surface of the electroconductive substrate. Thereafter a solution containing 0.5% by weight or less of a multifunctional aldehyde and a predetermined amount of an oxidase capable of producing hydrogen peroxide (H.sub.2 O.sub.2) is applied on the surface of the previously formed crosslinked albumin layer to form a H.sub.2 O.sub.2 productive oxidase containing layer.

4. 发明授权

US5992221A Concentration measuring apparatus 失效
标题翻译：浓度测定仪
公开(公告)号：US5992221A
公开(公告)日：1999-11-30
申请号：US703700
申请日：1996-08-27
申请人： Akio Kariyone , Yoshio Hashizume , Ryuzo Hayashi
发明人： Akio Kariyone , Yoshio Hashizume , Ryuzo Hayashi
IPC分类号： G01N1/00 , G01N1/38 , G01N1/16 , G01N15/06
CPC分类号： G01N1/38 , G01N35/1095
摘要： Pipes provided with each switching valve in intermediate portions thereof for feeding sample solutions to a separation mechanism are joined by a multi-way joint, to reach the separation mechanism through a pump. When all valves are closed while driving the pump and thereafter one valve is opened, all bubbles etc. downstream the opened valve are swept and eliminated due to abrupt flow of a liquid since a portion downstream the valve has been decompressed. In switching of a sample in sample measurement, a valve of a pipe for a completely measured sample is left open while a valve of a pipe for a next sample is opened, and then the valve of the pipe for the previously completely measured sample is closed after a while. Thus, pressure fluctuation following sample switching is avoided.
摘要翻译：在其中间部分设置有用于将样品溶液供给到分离机构的每个切换阀的管道通过多向接头连接，以通过泵到达分离机构。当所有阀在驱动泵时关闭，此后一个阀打开，所以打开的阀下游的所有气泡等均由于液体的急剧流动被扫除并消除，因为阀下游的部分已被减压。在样品测量中切换样品时，用于完全测量样品的管道的阀门打开，而用于下一个样品的管道的阀门打开，然后用于先前完全测量的样品的管道阀门关闭过了一会儿。因此，避免了样品切换后的压力波动。

5. 发明授权

US5225321A Measuring apparatus using enzyme electrodes and the method thereof 失效
标题翻译：使用酶电极的测量装置及其方法
公开(公告)号：US5225321A
公开(公告)日：1993-07-06
申请号：US727341
申请日：1991-07-08
申请人： Ryuzo Hayashi , Akio Kariyone , Yoshio Hashizume
发明人： Ryuzo Hayashi , Akio Kariyone , Yoshio Hashizume
IPC分类号： C12Q1/00 , G01N27/403
CPC分类号： C12Q1/001 , C12Q1/005 , C12Q1/006
摘要： In the case of measuring concentration of, for example, glucose as a first substance to be measured, and that of sucrose as a second substance to be measured, using a measuring apparatus, the apparatus is provided with a first enzyme electrode, a dilution pipeline situated at the downstream of the electrode and for diluting the substances, and a second enzyme electrode situated at the downstream of the dilution pipeline. A sample, which contains the first and second substances, is poured intermittently into a constant flow of a buffer solution. The first enzyme electrode detects the first substance. The second enzyme electrode, which comprises an immobilized enzyme catalizing the conversion of the second substance in to the first substance, detects the first substance, which is originally contained in the sample as well as is formed on the second enzyme electrode. The concentration of the second substance in the sample can be determined by calculating about the outputs of the first and second enzyme electrodes.
摘要翻译：在测量作为第一待测物质的葡萄糖的浓度和作为第二待测物质的蔗糖的浓度的情况下，使用测量装置，设置有第一酶电极，稀释管线位于电极的下游并用于稀释物质，以及位于稀释管道下游的第二酶电极。将含有第一和第二物质的样品间歇地倒入缓冲溶液的恒定流中。第一酶电极检测第一物质。第二酶电极，其包含催化第二物质向第一物质转化的固定化酶，检测原样包含在样品中的第一物质，并形成在第二酶电极上。可以通过计算第一和第二酶电极的输出来确定样品中第二物质的浓度。

6. 发明授权

US5221521A Sample liquid dilution system for analytical measurements 失效
标题翻译：用于分析测量的样品液体稀释系统
公开(公告)号：US5221521A
公开(公告)日：1993-06-22
申请号：US735911
申请日：1991-07-25
申请人： Yoshio Hashizume , Akio Kariyone , Ryuzo Hayashi
发明人： Yoshio Hashizume , Akio Kariyone , Ryuzo Hayashi
IPC分类号： G01N1/38 , G01N35/08
CPC分类号： G01N1/38 , G01N35/085 , Y10T436/117497 , Y10T436/118339 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： A liquid dilution system which dilutes a sample to be analyzed with a carrier to supply a diluted sample-containing liquid to an analytical measurement apparatus of flow type. The inventive dilution system features simplicity and versatility as compared to conventional automatic apparatus with robot concept, and the present system comprises: a first carrier pump for feeding a first carrier; a sample injection unit for injecting the sample into the first carrier; a main passage for flowing a liquid from the sample injection unit to a detector unit of an analytical measurement apparatus; a branching device located downstream of the sample injection unit for forming a branched passage to remove a liquid mass partially from the main passage; a confluence unit located downstream of the branching unit for confluencing a second carrier; and a second carrier pump for feeding the second carrier to the confluence unit. Dilution and mixing of the sample with a carrier is done twice, once at the sample injection and then again at confluence.

7. 发明授权

US5190872A Immobilized alcohol utidase for use in an alcohol measuring apparatus 失效
标题翻译：用于酒精测量仪器的稳定的酒精使用
公开(公告)号：US5190872A
公开(公告)日：1993-03-02
申请号：US573066
申请日：1990-08-27
申请人： Yoshio Hashizume , Akio Kariyone , Ryuzo Hayashi , Minako Oka
发明人： Yoshio Hashizume , Akio Kariyone , Ryuzo Hayashi , Minako Oka
IPC分类号： G01N27/327 , C12N11/00 , C12N11/06 , C12N11/14 , C12Q1/00 , G01N27/416
CPC分类号： C12N11/06 , C12Q1/003 , Y10S435/817
摘要： Alcohol oxidase is immobilized by bonding an aminosilane coupling agent to a carrier, bonding a multifunctional aldehyde such as glutaraldehyde to an amino group of the aminosilane, washing the carrier to remove free multifunctional aldehyde and bonding alcohol oxidase to the multifunctional aldehyde bonded to the aminosilane. Washing to remove free multifunctional aldehyde enables immobilizing a one molecule thickness of alcohol oxidase on the carrier since free multifunctional aldehyde is not present to cross-link alcohol oxidase molecules together. A thin layer of alcohol oxidase is advantageous when assaying for alcohol since a thin layer does not retain hydrogen peroxide that can deactivate alcohol oxidase. The carrier preferably contains hydroxyl groups and is porous, and can be a silicate-containing carrier such as diatomaceous earth or fire brick. The immobilized alcohol oxidase is used in a column as part of an apparatus for measuring alcohol or as part of an apparatus containing immobilized glucose oxidase for measuring glucose and alcohol.

8. 发明授权

US5081015A Enzyme electrode and method for determination of alcohol content using the same 失效
标题翻译：酶的电极和使用该醇的醇的测定方法
公开(公告)号：US5081015A
公开(公告)日：1992-01-14
申请号：US401132
申请日：1989-08-30
申请人： Ryuzo Hayashi , Akio Kariyone , Yoshio Hashizume
发明人： Ryuzo Hayashi , Akio Kariyone , Yoshio Hashizume
IPC分类号： C12Q1/00
CPC分类号： C12Q1/005 , Y10S435/817 , Y10S435/921 , Y10S435/938
摘要： An enzyme electrode possessing an immobilized enzyme membrane or an immobilized enzyme layer composed by applying a solution containing alcohol oxidase and a crosslinking agent, in which the solution further contains reduced glutathione.According to the invention it is possible to immobilize alcohol oxidase stably, and an excellent immobilized enzyme electrode for alcohol measurement is obtained. Furthermore, by this invention, a highly sensitive and stable measuring method is conducted quite easily.

9. 发明授权

US5248597A Method and apparatus for analyzing starch and related carbohydrates 失效
标题翻译：分析淀粉和相关碳水化合物的方法和装置
公开(公告)号：US5248597A
公开(公告)日：1993-09-28
申请号：US682953
申请日：1991-04-10
申请人： Ryuzo Hayashi , Yoshio Hasizume , Akio Kariyone
发明人： Ryuzo Hayashi , Yoshio Hasizume , Akio Kariyone
IPC分类号： C12M1/40 , C12Q1/00 , C12Q1/26 , C12Q1/40 , C12Q1/54 , G01N33/66
CPC分类号： G01N33/66 , C12Q1/005
摘要： A method and apparatus for the determination of the average molecular weight, average chain length or dextrose equivalent of starch and related carbohydrates containing glucose units. The starch is analyzed by determining the free glucose amount (Gf) contained in starch or related carbohydrate sample, determining a gross glucose amount (Gt) rendered by hydrolysis of starch or related carbohydrates, and further determining a glucose amount (Gr) rendered by hydrolysis of a reduced product which is obtained by reduction of reducing terminals contained in starch or related carbohydrate.The average molecular weight, average chain length or dextrose equivalent of the starch is determined using the formulas stated below from the measured amounts of the free glucose amount (Gf), and the glucose amount (Gr). The apparatus preferably utilized immobilized enzymes to conduct the hydrolysis of the starch sample, contains a device for recognizing and storing the measured values and computed data, and contains further a device for computing average molecular weight (Ma), average chain length (Dp) or dextrose equivalent value (DE) according to the following expressions: Dp=2.multidot.Gt/(Gt-Gr+Gf), Ma=18(19.multidot.Gt+Gf-Gr)/(Gt-Gr+Gf), DE=1000(Gt-Gr+Gf)/(19.multidot.Gt-Gr+Gf), or DE=1000/(9.multidot.Dp+1).

10. 发明授权

US5242793A Selective permeable membrane and electrode using the same 失效
标题翻译：选择性渗透膜和使用其的电极
公开(公告)号：US5242793A
公开(公告)日：1993-09-07
申请号：US490709
申请日：1990-03-07
申请人： Akio Kariyone , Ryuzo Hayashi , Yoshio Hashizume
发明人： Akio Kariyone , Ryuzo Hayashi , Yoshio Hashizume
IPC分类号： B01D69/14 , C12N11/02 , C12N11/10 , C12Q1/00 , G01N27/40
CPC分类号： C12Q1/003 , B01D69/141 , C12N11/02 , C12N11/10 , C12Q1/002 , G01N27/3271 , Y10S435/817
摘要： An electrode comprising an electrically conductive base, and a selectively permeable membrane which is produced by forming a membrane from a mixed solution comprising (a) albumin, (b) at least one type of crosslinking agent and (c) chitosan. The selectively permeable membrane is useful, e.g., in conjuction with an electrode. An enzyme electrode is also provided having the selectively permeable membrane disposed on the electrode and an immobilized enzyme membrane disposed on the selectively permeable membrane. The immobilized enzyme membrane is prepared by incorporating a carboxylate into the solution containing the enzyme and an aldehyde as the crosslinking agent. The crosslinking is effected by heating or drying.

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