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1. 发明申请

US20070155013A1 Pluripotent stem cell growing method 有权
标题翻译：多能干细胞生长方法
公开(公告)号：US20070155013A1
公开(公告)日：2007-07-05
申请号：US10593831
申请日：2005-03-23
申请人： Toshihiro Akaike , Keiichi Fukuda , Masato Nagaoka , Uichi Koshimizu
发明人： Toshihiro Akaike , Keiichi Fukuda , Masato Nagaoka , Uichi Koshimizu
IPC分类号： C12N15/09 , C12N5/06 , C12N5/08
CPC分类号： C12N5/0606 , C12N2533/50
摘要： A novel growing method is provided for pluripotent stem cells such as ES cells. The method of the invention is a pluripotent stem cell growing method and gene transfer method in which pluripotent stem cells are cultured under conditions that maintain their undifferentiated state and pluripotency, the method being characterized by using a liquid medium and a culturing vessel having immobilized or coated on a substrate solid phase surface a molecule which is adhesive to the pluripotent stem cells in a fixed concentration, to grow the pluripotent stem cells in a dispersed state while maintaining their undifferentiated state and pluripotency, without using feeder cells, or to transfer and express a gene therein.
摘要翻译：为多能干细胞（如ES细胞）提供了一种新的生长方法。本发明的方法是多能干细胞生长方法和基因转移方法，其中多能干细胞在保持其未分化状态和多能性的条件下培养，该方法的特征在于使用液体培养基和具有固定或涂覆的培养容器在基质固相表面上以固定浓度与多能干细胞粘合的分子，以分散状态生长多能干细胞，同时保持其未分化状态和多能性，而不使用饲养细胞，或转移和表达基因。

2. 发明授权

US07892835B2 Pluripotent stem cell growing method 有权
标题翻译：多能干细胞生长方法
公开(公告)号：US07892835B2
公开(公告)日：2011-02-22
申请号：US10593831
申请日：2005-03-23
申请人： Toshihiro Akaike , Keiichi Fukuda , Masato Nagaoka , Uichi Koshimizu
发明人： Toshihiro Akaike , Keiichi Fukuda , Masato Nagaoka , Uichi Koshimizu
IPC分类号： C12N15/09 , C12N5/06 , C12N5/08
CPC分类号： C12N5/0606 , C12N2533/50
摘要： A novel growing method is provided for pluripotent stem cells such as ES cells. The method of the invention is a pluripotent stem cell growing method and gene transfer method in which pluripotent stem cells are cultured under conditions that maintain their undifferentiated state and pluripotency, the method being characterized by using a liquid medium and a culturing vessel having immobilized or coated on a substrate solid phase surface a molecule which is adhesive to the pluripotent stem cells in a fixed concentration, to grow the pluripotent stem cells in a dispersed state while maintaining their undifferentiated state and pluripotency, without using feeder cells, or to transfer and express a gene therein.
摘要翻译：为多能干细胞（如ES细胞）提供了一种新的生长方法。本发明的方法是多能干细胞生长方法和基因转移方法，其中多能干细胞在保持其未分化状态和多能性的条件下培养，该方法的特征在于使用液体培养基和具有固定或涂覆的培养容器在基质固相表面上以固定浓度与多能干细胞粘合的分子，以分散状态生长多能干细胞，同时保持其未分化状态和多能性，而不使用饲养细胞，或转移和表达基因。

3. 发明授权

US5206318A Styrene derivatives having N-acetylchito-oligosaccharide chains and method for the same 失效
标题翻译：具有N-乙酰氨基寡糖链的苯乙烯衍生物及其制备方法
公开(公告)号：US5206318A
公开(公告)日：1993-04-27
申请号：US855159
申请日：1992-03-20
申请人： Kazukiyo Kobayashi , Toshihiro Akaike , Hosei Shinoda , Kazushi Morimoto
发明人： Kazukiyo Kobayashi , Toshihiro Akaike , Hosei Shinoda , Kazushi Morimoto
IPC分类号： C07H15/04 , C07H15/10 , C07H15/18 , C08F12/26 , C12N5/00
CPC分类号： C07H15/04 , C07H15/10 , C07H15/18 , C08F12/26 , C12N5/0068
摘要： A styrene derivative carrying N-acethylchito-oligosaccharide chains, a polystyrene derivative carrying N-acetylchito-oligosaccharide chains on the side chains as well as a method for preparing these compounds are herein disclosed. The method for preparing the styrene derivative comprises reacting N-acetylchito-oligosaccharide lactone with vinylbenzylamine or a derivative thereof while the method for preparing the polystyrene derivative comprises polymerizing the styrene derivative. These derivatives can be employed as biomedical materials, in particular as materials for cell culture.
摘要翻译：本文公开了携带N-乙酰壳寡糖链的苯乙烯衍生物，侧链上携带N-乙酰基 - 寡糖链的聚苯乙烯衍生物以及这些化合物的制备方法。制备苯乙烯衍生物的方法包括使N-乙酰基壳寡糖内酯与乙烯基苄胺或其衍生物反应，而制备聚苯乙烯衍生物的方法包括使苯乙烯衍生物聚合。这些衍生物可以用作生物医学材料，特别是作为细胞培养的材料。

4. 发明授权

US06372194B1 MRI contrast media recognizing minor environmental changes 失效
标题翻译： MRI造影剂识别轻微的环境变化
公开(公告)号：US06372194B1
公开(公告)日：2002-04-16
申请号：US09381176
申请日：1999-09-17
申请人： Toshihiro Akaike , Masahito Mikawa , Atsushi Maruyama , Naoto Miwa
发明人： Toshihiro Akaike , Masahito Mikawa , Atsushi Maruyama , Naoto Miwa
IPC分类号： A61B5055
CPC分类号： A61K49/085 , A61K49/12 , A61K49/128
摘要： This relates to a contrast medium containing a complex of a gadolinium (Gd) type contrast agent and a polymer. More particularly, the contrast medium wherein the polymer capable of phase transit in response to environmental changes, particularly, pH, light or temperature, to develop a different water solubility, and a method for imaging by the use of this contrast medium. By binding a polymer responsive to microenvironmental changes with a Gd type MRI contrast agent, on-off switching of MRI imaging capability is enabled, wherein imaging occurs only on the target sites. Consequently, a highly specific MRI contrast medium can be obtained, whereby a highly specific MRI diagnosis is made possible, wherein only the target sites such as a tumor and a particular site are imaged and otherwise at the site where imaging is not necessary.
摘要翻译：这涉及含有钆（Gd）型造影剂和聚合物的复合物的造影剂。更具体地，其中响应于环境变化，特别是pH，光或温度而相转移的聚合物以形成不同的水溶性的造影剂，以及通过使用该造影剂进行成像的方法。通过用Gd型MRI造影剂结合响应于微环境变化的聚合物，能够进行MRI成像能力的开 - 关切换，其中成像仅在靶位点上发生。因此，可以获得高度特异性的MRI造影剂，由此可以进行高度特异性的MRI诊断，其中只有目标部位如肿瘤和特定部位成像，否则在不需要成像的部位进行成像。

5. 发明授权

US09745549B2 Cell culture substrate, and cell culturing method using the substrate and method for inducing differentiation of pluripotent stem cells using the substrate 有权
公开(公告)号：US09745549B2
公开(公告)日：2017-08-29
申请号：US13865642
申请日：2013-04-18
申请人： SOMAR Corp. , Toshihiro Akaike
发明人： Amranul Haque , Masato Nagaoka , Toshihiro Akaike
IPC分类号： A61K38/00 , C12N5/0797 , C12N5/079 , C12N5/0735 , C12N5/074
CPC分类号： C12N5/0623 , C12N5/0606 , C12N5/0618 , C12N5/0696 , C12N2506/02 , C12N2506/45 , C12N2533/30 , C12N2533/50
摘要： [Problem] To provide a cell culture substrate, and a cell culturing method using the substrate and a method for inducing differentiation of pluripotent stem cells using the substrate, which allow culturing of pluripotent stem cells and allow differentiation of pluripotent stem cells into a specified cell species, particularly neural and neural progenitor cells, at a high purity.[Means for Solution] A cell culture substrate, characterized in that, onto the surface, one or more selected from the group consisting of N-cadherin, a fusion protein comprising an entire or partial region of N-cadherin, and a fusion protein comprising an entire or partial region of a protein homologous to N-cadherin are immobilized or coated.

6. 发明申请

US20070077306A1 Apatite particle, method of producing the same, apatite particle-gene complex, and method of gene transfection 审中-公开
标题翻译：磷灰石颗粒，其制备方法，磷灰石颗粒 - 基因复合物和基因转染方法
公开(公告)号：US20070077306A1
公开(公告)日：2007-04-05
申请号：US10584722
申请日：2004-12-27
申请人： Toshihiro Akaike , Ezharul Chowdhury
发明人： Toshihiro Akaike , Ezharul Chowdhury
IPC分类号： A61K48/00 , C12N15/87 , A61K9/14
CPC分类号： C12N15/87 , C01B25/32
摘要： When a solution of an inorganic phosphoric acid, added to with calcium ions and magnesium ions, is incubated for a preset time duration, an apatite particle obtained is reduced in size, depending on the concentration of the magnesium ions. By employing an apatite particle, for which the concentration of the magnesium ions and the incubation time duration are controlled adequately, it is possible to improve the gene transfection efficiency into the cells and the gene expression efficiency in the cells.
摘要翻译：当加入钙离子和镁离子的无机磷酸溶液温育预设的时间时，根据镁离子的浓度，获得的磷灰石颗粒尺寸减小。通过使用磷灰石颗粒，镁离子的浓度和孵育时间适当地控制，可以提高细胞的基因转染效率和细胞中的基因表达效率。

7. 发明授权

US6150461A Carriers targettable to organ 失效
标题翻译：携带者指向器官
公开(公告)号：US6150461A
公开(公告)日：2000-11-21
申请号：US863564
申请日：1997-05-27
申请人： Yoshiyuki Takei , Atsushi Maruyama , Toshihiro Akaike , Sunao Kawano , Shigetoshi Okumura , Masayuki Nogawa , Shoichiro Asayama
发明人： Yoshiyuki Takei , Atsushi Maruyama , Toshihiro Akaike , Sunao Kawano , Shigetoshi Okumura , Masayuki Nogawa , Shoichiro Asayama
IPC分类号： A61K38/14 , C08G63/48 , C07K1/00 , C08G63/91
CPC分类号： A61K38/14
摘要： A carrier for delivering a foreign substance to a target organ which comprises a graft copolymer in which hyaluronic acid is grafted on a polymer composing main chain, wherein the main chain has a part capable of binding to the foreign substance electrostatically, and contains one or more monomer unit having side chain with amino or imino group capable of coupling to hyarulonic acid, which is useful for delivering a foreign substance to a target organ, especially liver.
摘要翻译：一种用于将异物输送到目标器官的载体，该载体包括其中透明质酸接枝在构成主链的聚合物上的接枝共聚物，其中所述主链具有能够静电结合异物的部分，并且包含一种或多种具有侧链的单体单元，其具有能够与hyarulonic酸偶联的氨基或亚氨基，其可用于将异物递送至靶器官，特别是肝。

8. 发明授权

US5124437A Galactosamine substitute of poly-.omega.-substituted-L-glutamic acid (or aspartic acid) 失效
标题翻译：聚（OMEGA）-SUBSTITUTED-L-谷氨酸（或偶氮酸）的乳糖胺替代物
公开(公告)号：US5124437A
公开(公告)日：1992-06-23
申请号：US690496
申请日：1991-04-23
申请人： Toshihiro Akaike , Ichirou Kitada , Megumi Kunou
发明人： Toshihiro Akaike , Ichirou Kitada , Megumi Kunou
IPC分类号： A61K38/00 , C07K14/00
CPC分类号： C07K14/00 , A61K38/00
摘要： A galactosamine substituted poly-.omega.-alkyl (or benzyl)-L-glutamic acid (or aspartic acid) is provided and comprises a polypeptide having the recurring unit represented by the formula: ##STR1## wherein X has a value of 60 to 250; n is 1 or 2; and R represents a lower alkyl group or benzyl group, in which a part or all of the peptide in said polypeptide is substituted by an .omega.-galactosamyl-L-glutamic acid (or aspartic acid) residue represented by the general formula: ##STR2## wherein n is as indicated.

9. 发明授权

US4836928A Separation method, separation device and separation apparatus for separating body fluid into respective components 失效
标题翻译：分离方法，用于将体液分离成各个部件的分离装置和分离装置
公开(公告)号：US4836928A
公开(公告)日：1989-06-06
申请号：US727905
申请日：1985-04-26
申请人： Juuro Aoyagi , Yuichi Yamamoto , Toshihiro Akaike , Yasuhiro Nozaki
发明人： Juuro Aoyagi , Yuichi Yamamoto , Toshihiro Akaike , Yasuhiro Nozaki
IPC分类号： A61M1/36 , A61K9/127 , A61K35/14 , B01D15/08 , B01D67/00 , B01D69/14 , C12N5/06 , G01N33/48 , G01N33/543 , G01N33/552
CPC分类号： G01N33/552 , B01D15/08 , B01D67/0088 , B01D69/141 , G01N33/48 , G01N33/54366 , B01D15/3804 , Y10T436/255
摘要： A body fluid is separated into respective components by passing the body fluid through a separation device. The separation device includes a lipid having lipophilic and hydrophilic groups, and a support immobilizing the lipid. The lipid is immobilized on the support such that only its hydrophilic groups contact the body fluid introduced in the separation device. Only body fluid components having a strong adherent property adhere to the hydrophilic groups of the lipid.
摘要翻译：通过使体液通过分离装置将体液分离成各个部件。分离装置包括具有亲脂性和亲水基团的脂质和固定脂质的载体。将脂质固定在载体上，使得只有其亲水基团与引入分离装置的体液接触。只有具有强粘附性的体液组分粘附于脂质的亲水基团。

10. 发明授权

US09295640B2 Pharmaceutical composition for treating cancer using carbonate apatite nanoparticles 有权
标题翻译：使用碳酸盐磷灰石纳米颗粒治疗癌症的药物组合物
公开(公告)号：US09295640B2
公开(公告)日：2016-03-29
申请号：US14010878
申请日：2013-08-27
申请人： Hirofumi Yamamoto , Toshihiro Akaike , Masaki Mori , Yuichiro Doki , Xin Wu , IJUNKAI
发明人： Hirofumi Yamamoto , Hiroyuki Nakanishi , Toshihiro Akaike , Masaki Mori , Yuichiro Doki , Xin Wu
IPC分类号： A61K48/00 , C12N15/11 , C07H21/02 , C07H21/04 , A61K9/00 , A61K9/51
CPC分类号： A61K9/0019 , A61K9/5115 , C12N15/111 , C12N2320/32
摘要： A principal object of the present invention is to provide a pharmaceutical composition that can produce a high antitumor effect by efficiently delivering a drug with antitumor activity to tumor tissues with the aid of carbonate apatite nanoparticles. The present invention provides a pharmaceutical composition including carbonate apatite nanoparticles with an average particle size of at most 50 nm containing a drug with antitumor activity and a pharmacologically acceptable solvent in which the carbonate apatite nanoparticles containing the drug are dispersed.
摘要翻译：本发明的主要目的是提供一种药物组合物，其可以借助于碳酸盐磷灰石纳米颗粒，有效地向肿瘤组织递送具有抗肿瘤活性的药物，从而产生高抗肿瘤效果。本发明提供一种药物组合物，其包含平均粒径为50nm以下的碳酸磷灰石纳米粒子，其含有具有抗肿瘤活性的药物和药物可接受的溶剂，其中分散有所述药物的碳酸盐磷灰石纳米颗粒。

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