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1. 发明授权

US6159688A Methods of producing polynucleotide variants 失效
公开(公告)号：US6159688A
公开(公告)日：2000-12-12
申请号：US40698
申请日：1998-03-18
申请人： Torben Vedel Borchert , Titus Kretzschmar , Joel R. Cherry , Jesper Vind
发明人： Torben Vedel Borchert , Titus Kretzschmar , Joel R. Cherry , Jesper Vind
IPC分类号： C12N9/20 , C12N9/28 , C12N9/56 , C12N15/10 , C12Q1/68
CPC分类号： C12N15/1093 , C12N9/20 , C12N9/2417 , C12N9/54 , C12N15/1027
摘要： A method for the construction of a library of recombined polynucleotides from a number of different starting single or double stranded parental DNA templates is disclosed, wherein the starting single or double stranded parental DNA templates represent discrete points in a population of genes encoding evolutionary or synthetic homologues of a peptide having homologies ranging over a broad spectrum from less than 15% to more than 80%, said population exhibiting at least one identification sequence, and whereby said genes are subjected to a gene shuffling procedure to generate shuffled mutants of said population of genes representing additional discrete points between those of said starting templates.

2. 发明授权

US06291165B1 Shuffling of heterologous DNA sequences 失效
标题翻译：改组异源DNA序列
公开(公告)号：US06291165B1
公开(公告)日：2001-09-18
申请号：US09040025
申请日：1998-03-17
申请人： Torben Vedel Borchert , Titus Kretzschmar , Joel R. Cherry
发明人： Torben Vedel Borchert , Titus Kretzschmar , Joel R. Cherry
IPC分类号： C12Q168
CPC分类号： C12N15/1027
摘要： The present invention relates to a new method of shuffling especially heterologous polynucleotide sequences, screening and/or selection of new recombinant proteins resulting therefrom having a desired biological activity, and especially to production and identification of novel proteases exhibiting desired properties.
摘要翻译：本发明涉及一种混合洗涤方法，特别是异源多核苷酸序列，筛选和/或选择由此产生的新的重组蛋白质具有所需的生物学活性，特别是涉及产生和鉴定出具有所需性质的新型蛋白酶。

3. 发明授权

US06368805B1 Methods of producing polynucleotide variants 失效
标题翻译：产生多核苷酸变体的方法
公开(公告)号：US06368805B1
公开(公告)日：2002-04-09
申请号：US09644953
申请日：2000-08-23
申请人： Torben Vedel Borchert , Titus Kretzschmar , Joel R. Cherry , Vind Jesper
发明人： Torben Vedel Borchert , Titus Kretzschmar , Joel R. Cherry , Vind Jesper
IPC分类号： C12Q168
CPC分类号： C12N15/1093 , C12N9/20 , C12N9/2417 , C12N9/54 , C12N15/1027
摘要： A method for the construction of a library of recombined polynucleotides from a number of different starting single or double stranded parental DNA templates is disclosed, wherein the starting single or double stranded parental DNA templates represent discrete points in a population of genes encoding evolutionary or synthetic homologues of a peptide having homologies ranging over a broad spectrum from less than 15% to more than 80%, said population exhibiting at least one identification sequence, and whereby said genes are subjected to a gene shuffling procedure to generate shuffled mutants of said population of genes representing additional discrete points between those of said starting templates.
摘要翻译：公开了从许多不同的起始单链或双链亲本DNA模板构建重组多核苷酸文库的方法，其中起始单链或双链亲本DNA模板代表编码进化或合成同源物的基因群体中的离散点具有在小于15％至大于80％的广谱范围内的同源性的肽，所述群体显示至少一种鉴定序列，并且其中所述基因经历基因改组程序以产生所述基因群体的混洗突变体代表所述起始模板之间的额外离散点。

4. 发明授权

US06541207B1 Methods for generating recombined polynucleotides 失效
标题翻译：产生重组多核苷酸的方法
公开(公告)号：US06541207B1
公开(公告)日：2003-04-01
申请号：US09687301
申请日：2000-10-13
申请人： Jesper Vind , Torben Vedel Borchert
发明人： Jesper Vind , Torben Vedel Borchert
IPC分类号： C12N1500
CPC分类号： C12N15/1093 , C12N15/1027
摘要： A method for in vitro construction of a library of recombined homologous polynucleotides from a number of different starting DNA templates and primers by induced template shifts during an polynucleotide synthesis is described, whereby A. extended primers are synthesized by a) denaturing the DNA templates b) annealing primers to the templates, c) extending the said primers by use of a polymerase, d) stop the synthesis, and e) separate the extended primers from the templates, B. a template shift is induced by a) isolating the extended primers from the templates and repeating steps A.b) to A.e) using the extended primers as both primers and templates, or b) repeating steps A.b) to A.e), C. this process is terminated after an appropriate number of cycles of process steps A. and B.a), A. and B.b), or combinations thereof. Optionally the polynucleotides are amplified in a standard PCR reaction with specific primers to selectively amplify homologous polynucleotides of interest.
摘要翻译：描述了在多核苷酸合成期间通过诱导的模板位移从许多不同的起始DNA模板和引物体外构建重组的同源多核苷酸文库的方法，其中A. 通过a）使DNA模板变性b）将引物退火到模板来合成延伸的引物，c）使用聚合酶扩增所述引物，d）停止合成，并且将扩展的引物与模板B分开。通过以下步骤诱导模板位移：a）使扩展引物与模板分离，并重复步骤A.b）至A.e），使用扩展引物作为引物和模板，orb）重复步骤A.b）至A.e），C。该方法在方法步骤A和B.a），A.和B.b）或其组合的适当数目的循环后终止。可选地，在与特异性引物的标准PCR反应中扩增多核苷酸以选择性扩增目的同源多核苷酸。

5. 发明授权

US06518042B1 Process for making DNA libraries in filamentous fungal cells using a novel cloned gene involved in the mismatch repair system of filamentous fungal cells 失效
标题翻译：使用参与丝状真菌细胞错配修复系统的新型克隆基因在丝状真菌细胞中制备DNA文库的方法
公开(公告)号：US06518042B1
公开(公告)日：2003-02-11
申请号：US09512250
申请日：2000-02-24
申请人： Torben Vedel Borchert , Lars Christiansen , Jesper Vind
发明人： Torben Vedel Borchert , Lars Christiansen , Jesper Vind
IPC分类号： C12P2102
CPC分类号： C07K14/38 , C12N15/80
摘要： A process for making DNA libraries in filamentous fungal cells using a novel cloned gene involved in the mismatch repair system of filamentous fungal cells.
摘要翻译：使用涉及丝状真菌细胞的错配修复系统的新的克隆基因在丝状真菌细胞中制备DNA文库的方法。

6. 发明授权

US08513175B2 Alpha-amylase mutants 有权
公开(公告)号：US08513175B2
公开(公告)日：2013-08-20
申请号：US13557313
申请日：2012-07-25
申请人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen
发明人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen
IPC分类号： C11D3/386 , C12N9/28
CPC分类号： C12N9/2417 , C11D3/386 , C12P19/02 , C12P19/14
摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to the parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.

7. 发明授权

US08323946B2 Alpha-amylase mutants 失效
标题翻译： α-淀粉酶突变体
公开(公告)号：US08323946B2
公开(公告)日：2012-12-04
申请号：US13161931
申请日：2011-06-16
申请人： Allan Svendsen , Henrik Bisgard-Frantzen , Torben Vedel Borchert
发明人： Allan Svendsen , Henrik Bisgard-Frantzen , Torben Vedel Borchert
IPC分类号： C12N9/00 , C12N9/30 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C11D3/386 , C12N9/2417
摘要： The present invention relates to a method of constructing a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and at least one altered property as compared to the parent alpha-amylase, comprises i) analyzing the structure of the parent Termamyl-like alpha-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like alpha-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like alpha-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like alpha-amylase variant, which as compared to the parent Termamyl-like alpha-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally, iii) testing the resulting Termamyl-like alpha-amylase variant with respect to the property in question.
摘要翻译：本发明涉及一种构建母体类戊酰样α-淀粉酶的变体的方法，该变体具有与母体α-淀粉酶相比具有α-淀粉酶活性和至少一种改变的性质，包括：i）分析鉴定至少一个氨基酸残基或类甲酰样α-淀粉酶结构的至少一个结构部分，所述氨基酸残基或结构部分被认为与改变其性质有关的相关性母体类戊酰样α-淀粉酶（根据结构或功能考虑进行评估），ii）构建与母体类戊酰样α-淀粉酶相比的类戊酰样α-淀粉酶变体，已经在在i）中鉴定的氨基酸残基或结构部分，以改变其性质，以及任选地，iii）相对于所讨论的性质测试所得的类戊酰样α-淀粉酶变体。

8. 发明授权

US08263368B2 Alpha-amylase mutants 有权
标题翻译： α-淀粉酶突变体
公开(公告)号：US08263368B2
公开(公告)日：2012-09-11
申请号：US12493860
申请日：2009-06-29
申请人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen , Helle Outtrup , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Nielsen , Lisbeth Hedegaard
发明人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen , Helle Outtrup , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Nielsen , Lisbeth Hedegaard
IPC分类号： C12N9/28 , C12S11/00 , C12P19/14
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38609 , C11D3/38618 , C12Y302/01001
摘要： The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).
摘要翻译：本发明涉及一种新的类戊酰样α-淀粉酶和包含2,3,4,5或6个或6个位置突变的类戊酰样α-淀粉酶。这些变体在酸性pH和/或低Ca 2+浓度（相对于亲本）下具有增加的热稳定性。本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体在酸性pH和/或低Ca 2+浓度（相对于亲本）中表现出增加的热稳定性。

9. 发明授权

US06673589B2 &agr;-amylase mutants 有权
标题翻译： α-淀粉酶突变体
公开(公告)号：US06673589B2
公开(公告)日：2004-01-06
申请号：US09769864
申请日：2001-01-25
申请人： Torben Vedel Borchert , Allan Svendsen , Carsten Andersen , Bjarne Nielsen , Torben Lauesgaard Nissen , Søren Kjærulff
发明人： Torben Vedel Borchert , Allan Svendsen , Carsten Andersen , Bjarne Nielsen , Torben Lauesgaard Nissen , Søren Kjærulff
IPC分类号： C12N928
CPC分类号： C12N9/2417 , C07K14/32 , C11D3/386 , C11D3/38609 , C11D3/38636 , C12Y302/01001
摘要： The invention relates to a variant of a parent Termamyl-like &agr;-amylase, which exhibits an alteration in at least one of the following properties relative to said parent &agr;-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.
摘要翻译：本发明涉及亲本的类戊酰样α-淀粉酶的变体，其相对于所述亲本α-淀粉酶表现出至少一种以下特性的改变：i）在8至10.5的pH下改善的pH稳定性; 和/或ii）在pH8至10.5下提高Ca 2+稳定性，和/或iii）在10至60℃的温度下提高比活性。

10. 发明授权

US08288144B2 Pectate lyase variants 有权
标题翻译：果胶裂解酶变体
公开(公告)号：US08288144B2
公开(公告)日：2012-10-16
申请号：US12481922
申请日：2009-06-10
申请人： Sanne Schroder Glad , Carsten Andersen , Torben Vedel Borchert , Katja Salomon Johansen , Henrik Frisner , Mads Eskelund Bjornvad , Thomas Thisted
发明人： Sanne Schroder Glad , Carsten Andersen , Torben Vedel Borchert , Katja Salomon Johansen , Henrik Frisner , Mads Eskelund Bjornvad , Thomas Thisted
IPC分类号： C12N9/88 , C11D3/386
CPC分类号： C12N9/88 , C11D3/38636
摘要： The present invention relates to pectate lyase variants exhibiting alterations relative to a parent enzyme exhibiting pectate lyase activity as its major enzymatic activity; to a method of producing such enzymes; and to methods for using such enzymes in the textile, detergent and cellulose fiber processing industries. Compared to the parent enzyme, the pectate lyase variants of the present invention exhibit improved stability in detergents.
摘要翻译：本发明涉及相对于显示出果胶裂解酶活性的亲本酶作为其主要酶活性的改变的果胶酸裂解酶变体; 涉及一种生产这种酶的方法; 以及在纺织，洗涤剂和纤维素纤维加工业中使用这些酶的方法。与亲本酶相比，本发明的果胶酸裂解酶变体表现出改进的洗涤剂稳定性。

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