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1. 发明授权

US07588671B2 Microfluidic treatment method and device 失效
标题翻译：微流体处理方法及装置
公开(公告)号：US07588671B2
公开(公告)日：2009-09-15
申请号：US10992770
申请日：2004-11-22
申请人： Tomoyuki Morita , Akiko Miya , Akira Fukuda , Motohiko Nomi , Katsunori Ichiki , Manabu Tsujimura , Shunsuke Shimizu
发明人： Tomoyuki Morita , Akiko Miya , Akira Fukuda , Motohiko Nomi , Katsunori Ichiki , Manabu Tsujimura , Shunsuke Shimizu
IPC分类号： G01N27/447 , B01D57/02
CPC分类号： B01L3/50273 , B01F5/061 , B01F5/0646 , B01F5/0647 , B01F5/0654 , B01F7/00291 , B01F11/0071 , B01F11/0266 , B01F13/0059 , B01F2005/0621 , B01J19/0093 , B01J2219/00783 , B01J2219/00889 , B01J2219/00891 , B01L3/502738 , B01L3/502746 , B01L2300/0816 , B01L2300/0867 , B01L2400/0421 , B01L2400/082 , G01N27/44704 , G01N27/44791
摘要： The present invention relates to a microchip apparatus using liquids. More specifically, the invention provides a liquid mixing apparatus comprising at least two microchannels for introducing liquids and a mixing microchannel that connects to the at least two liquid-introducing microchannels, wherein the liquids are transported from the respective liquid-introducing microchannels toward the mixing microchannel, the apparatus further comprising means for enhancing the mixing of the liquids that converge in the mixing microchannel. The invention also provides an electrophoretic apparatus and a microchip electrophoretic apparatus for denaturing gradient gel electrophoresis.
摘要翻译：本发明涉及使用液体的微芯片装置。更具体地，本发明提供了一种液体混合装置，其包括用于引入液体的至少两个微通道和连接至少两个液体引入微通道的混合微通道，其中液体从相应的液体引入微通道转移到混合微通道该装置还包括用于增强会聚在混合微通道中的液体的混合的装置。本发明还提供了用于变性梯度凝胶电泳的电泳装置和微芯片电泳装置。

2. 发明申请

US20050161326A1 Microfluidic treatment method and device 失效
标题翻译：微流体处理方法及装置
公开(公告)号：US20050161326A1
公开(公告)日：2005-07-28
申请号：US10992770
申请日：2004-11-22
申请人： Tomoyuki Morita , Akiko Miya , Akira Fukuda , Motohiko Nomi , Katsunori Ichiki , Manabu Tsujimura , Shunsuke Shimizu
发明人： Tomoyuki Morita , Akiko Miya , Akira Fukuda , Motohiko Nomi , Katsunori Ichiki , Manabu Tsujimura , Shunsuke Shimizu
IPC分类号： B01F5/06 , B01F7/00 , B01F11/00 , B01F11/02 , B01F13/00 , B01J19/00 , B01L3/00 , G01N27/447 , G01N27/453
CPC分类号： B01L3/50273 , B01F5/061 , B01F5/0646 , B01F5/0647 , B01F5/0654 , B01F7/00291 , B01F11/0071 , B01F11/0266 , B01F13/0059 , B01F2005/0621 , B01J19/0093 , B01J2219/00783 , B01J2219/00889 , B01J2219/00891 , B01L3/502738 , B01L3/502746 , B01L2300/0816 , B01L2300/0867 , B01L2400/0421 , B01L2400/082 , G01N27/44704 , G01N27/44791
摘要： The present invention relates to a microchip apparatus using liquids. More specifically, the invention provides a liquid mixing apparatus comprising at least two microchannels for introducing liquids and a mixing microchannel that connects to the at least two liquid-introducing microchannels, wherein the liquids are transported from the respective liquid-introducing microchannels toward the mixing microchannel, the apparatus further comprising means for enhancing the mixing of the liquids that converge in the mixing microchannel. The invention also provides an electrophoretic apparatus and a microchip electrophoretic apparatus for denaturing gradient gel electrophoresis.
摘要翻译：本发明涉及使用液体的微芯片装置。更具体地，本发明提供了一种液体混合装置，其包括用于引入液体的至少两个微通道和连接至少两个液体引入微通道的混合微通道，其中液体从相应的液体引入微通道转移到混合微通道该装置还包括用于增强会聚在混合微通道中的液体的混合的装置。本发明还提供了用于变性梯度凝胶电泳的电泳装置和微芯片电泳装置。

3. 发明申请

US20080166770A1 METHOD AND APPARATUS FOR AMPLIFYING AND SYNTHESISIZING NUCLEIC ACID WITH DENATURANT 审中-公开
标题翻译：用于放大和合成核酸的方法和装置与脱氧核糖核酸
公开(公告)号：US20080166770A1
公开(公告)日：2008-07-10
申请号：US11864142
申请日：2007-09-28
申请人： Tomoyuki MORITA , Takashi Matsumura , Akiko Miya , Motohiko Nohmi , Shunsuke Shimizu
发明人： Tomoyuki MORITA , Takashi Matsumura , Akiko Miya , Motohiko Nohmi , Shunsuke Shimizu
IPC分类号： C12P19/34
CPC分类号： C12P19/34
摘要： The invention relates generally to the field of treating a nucleic acid. More particularly, the invention provides a method for amplifying a nucleic acid and an apparatus for amplifying a nucleic acid, as well as method and apparatus for synthesizing nucleic acid to be used for the amplification.
摘要翻译：本发明一般涉及处理核酸的领域。更具体地，本发明提供了用于扩增核酸的方法和用于扩增核酸的装置，以及用于合成用于扩增的核酸的方法和装置。

4. 发明申请

US20090294287A1 MICROCHIP ELECTROPHORESIS METHOD AND DEVICE 审中-公开
标题翻译： MICROCHIP电泳方法和装置
公开(公告)号：US20090294287A1
公开(公告)日：2009-12-03
申请号：US11915491
申请日：2006-05-10
申请人： Tomoyuki Morita , Takashi Matsumura , Akiko Miya , Hiroyuki Yamada
发明人： Tomoyuki Morita , Takashi Matsumura , Akiko Miya , Hiroyuki Yamada
IPC分类号： C07K1/26
CPC分类号： G01N27/44704 , G01N27/44747 , G01N27/44791
摘要： An separation method comprising a temperature control process useful for microchip electrophoresis such as microchip DGGE is provided along with a device therefor.The present invention relates to a microchip electrophoresis method for separating double-stranded nucleic acids by means of differences in nucleotide sequence while maintaining a preset temperature, wherein the temperature during separation of double-stranded nucleic acids in an separation region comprising an separation microchannel is controlled to within ±2.5° C. of the preset temperature.
摘要翻译：提供了包括用于微芯片电泳的微控制器电路的温度控制过程的分离方法，如微芯片DGGE及其装置。本发明涉及一种用于通过核苷酸序列差异分离双链核酸同时保持预设温度的微芯片电泳方法，其中在包含分离微通道的分离区域中双链核酸分离过程中的温度受到控制到预设温度的±2.5°C以内。

5. 发明申请

US20090297890A1 Anode for Bioelectric Power Generation And Power Generation Method And Apparatus Utilizing Same 审中-公开
标题翻译：生物发电阳极和发电方法及其利用方法
公开(公告)号：US20090297890A1
公开(公告)日：2009-12-03
申请号：US11992678
申请日：2006-09-26
申请人： Tatsuo Shimomura , Masanori Adachi , Makoto Komatsu , Akiko Miya
发明人： Tatsuo Shimomura , Masanori Adachi , Makoto Komatsu , Akiko Miya
IPC分类号： H01M8/16 , B05D5/12
CPC分类号： H01M8/16 , H01M4/86 , H01M4/8657 , H01M4/8807 , H01M4/8817 , H01M8/0245 , H01M8/04186 , H01M2004/8684 , Y02E60/527
摘要： A method and a device for obtaining electric energy efficiently from a hydrous organic substance by suppressing the activation overvoltage of an anode low and thereby obtaining a sufficiently low anode potential. The power generating device comprises an anaerobic region (4) including microorganisms which can grow under anaerobic conditions, solution or suspension containing an organic substance, an electron mediator and an anode (1), an aerobic region (5) including molecular oxygen and a cathode (3), and a diaphragm (2) defining the anaerobic region (4) and the aerobic region (5), wherein a closed circuit (6) is formed by connecting the anode (1) and the cathode (3) electrically with a power utilization apparatus, and oxidation reaction of microorganisms using the organic substance in the anaerobic region (4) as electron donor and a reduction reaction using oxygen in the aerobic region (5) as electron acceptor are utilized. The anode (1) includes a conductive substrate having a surface coated at least partly with a hydrophilic polymer layer, an electron mediator is introduced into the hydrophilic polymer layer with chemical bond, and the anode (1) has a standard electrode potential (E0′) at pH 7 in a range of −0.13 V to −0.28 V.
摘要翻译：通过将阳极的活化过电压抑制得低，从而获得足够低的阳极电位，从含水有机物质有效地获得电能的方法和装置。发电装置包括厌氧区（4），其包括能够在厌氧条件下生长的微生物，包含有机物质的溶液或悬浮液，电子介体和阳极（1），包含分子氧和阴极的需氧区（5）（3）和限定所述厌氧区（4）和好氧区（5）的隔膜（2），其中通过将阳极（1）和阴极（3）电连接而形成闭合回路（6）使用厌氧区（4）中的有机物质作为电子给体的微生物的氧化反应和作为电子受体的需氧区（5）中的氧的还原反应。阳极（1）包括具有至少部分地具有亲水性聚合物层的表面的导电性基材，通过化学键将电子介体引入亲水性聚合物层，阳极（1）具有标准电极电位（E0' ）在pH7下在-0.13V至-0.28V的范围内

6. 发明申请

US20090142627A1 Biological Power Generator, and Method of Treating Organic Solid Pollutant-Containing Waste, a Method of Treating Organic Polymeric Substance-Containing Wastewater, a Method of Treating Organic Substance-Containing Wastewater, as Well as Apparatuses for Implementing These Treatment Methods 审中-公开
标题翻译：生物发电机及处理含有机固体污染物的废物的处理方法，含有机聚合物废水的处理方法，含有机物废水的处理方法以及实施这些处理方法的设备
公开(公告)号：US20090142627A1
公开(公告)日：2009-06-04
申请号：US11992666
申请日：2006-09-27
申请人： Tatsuo Shimomura , Masanori Adachi , Akiko Miya
发明人： Tatsuo Shimomura , Masanori Adachi , Akiko Miya
IPC分类号： H01M8/16 , C02F11/02 , H01M8/04
CPC分类号： H01M8/16 , C02F1/283 , C02F1/46 , C02F1/52 , C02F3/005 , C02F3/12 , C02F3/286 , C02F3/30 , C02F3/342 , C02F2101/30 , C02F2103/20 , C02F2103/32 , H01M8/06 , H01M8/225 , H01M2004/8684 , Y02E60/527 , Y02W10/15
摘要： Disclosed are a biological power generator comprising an anaerobic region containing microorganisms capable of growth under anaerobic conditions and an anode having an electron mediator immobilized thereon and having a standard electrode potential (E0′) in a range of −0.13 V to −0.28 V at pH 7, an aerobic region containing molecular oxygen and a cathode, and a diaphragm that defines the anaerobic region and the aerobic region, as well as a method of treating organic waste by making use of the biological power generator.
摘要翻译：公开了一种生物发电装置，其包括含有能够在厌氧条件下生长的微生物的厌氧区域和固定有电子介体的阳极，并且在pH为-0.13V至-0.28V的范围内具有标准电极电位（E0'）为-0.13V至-0.28V 7，含有分子氧和阴极的需氧区域和限定厌氧区域和需氧区域的隔膜以及利用生物发电机处理有机废弃物的方法。

7. 发明授权

US5792650A Method for treating waste water 失效
标题翻译：废水处理方法
公开(公告)号：US5792650A
公开(公告)日：1998-08-11
申请号：US556636
申请日：1995-11-13
申请人： Hisao Ohtake , Akiko Miya , Hisashi Shinjo
发明人： Hisao Ohtake , Akiko Miya , Hisashi Shinjo
IPC分类号： C02F3/12 , C02F3/34 , C02F3/00 , C02F11/02
CPC分类号： C02F3/1215 , C02F3/34 , Y02W10/15
摘要： The present invention relates to a method of treatment of waste water which is characterized by removal of both organic substances and phosphorus from phosphorus-containing organic waste water by novel microorganisms that are fortified in phosphorus-incorporating and/or accumulating abilities. The method for treatment of waste water according to the present invention allows to efficiently remove both organic substances and phosphorus from organic waste water by genetically-engineered microorganisms that have acquired improved abilities in phosphorus incorporation and/or accumulation by genetic transformation using one or more vector plasmids carrying, together with a self-replicative DNA fragment, one of the DNA fragment combinations which include at least one member of the group of (a) a DNA fragment containing a gene involved in phosphate transport through cell membranes; (b) a DNA fragment containing a polyphosphate kinase structure gene; and (c) a DNA fragment containing a structure gene of an enzyme involved in intracellular ATP supply, excluding the sole use of the DNA fragment containing a polyphosphate kinase structure gene.
摘要翻译：本发明涉及一种处理废水的方法，其特征在于通过以磷掺入和/或累积能力强化的新型微生物除去含磷有机废水中的有机物质和磷。根据本发明的废水的处理方法允许通过遗传工程化的微生物有效地从有机废水中除去有机物质和磷，这些微生物通过使用一种或多种载体的遗传转化获得了磷掺入和/或积累的改进能力携带与自身复制DNA片段一起的一个DNA片段组合，其中包括以下组中的至少一个成员：（a）含有参与通过细胞膜的磷酸盐转运的基因的DNA片段; （b）含有多磷酸激酶结构基因的DNA片段; （c）含有参与细胞内ATP供应的酶的结构基因的DNA片段，不包括含有多磷酸激酶结构基因的DNA片段的唯一用途。

8. 发明授权

US4431543A Method of removing phosphorus from organic waste liquids 失效
标题翻译：从有机废液中除磷的方法
公开(公告)号：US4431543A
公开(公告)日：1984-02-14
申请号：US366302
申请日：1982-04-07
申请人： Yoshitaka Matsuo , Toshihiro Tanaka , Akiko Miya
发明人： Yoshitaka Matsuo , Toshihiro Tanaka , Akiko Miya
IPC分类号： C02F5/14 , C02F3/12 , C02F3/30 , C02F9/00
CPC分类号： C02F3/1215 , C02F3/308 , C02F2301/043 , Y02W10/15 , Y10S210/903 , Y10S210/906
摘要： Phosphorus, nitrogen and BOD are removed from organic waste liquid containing the same by mixing the organic waste liquid with activated sludge which is substantially free of oxygen, nitric acid and nitrous acid and subjecting the mixture to the action of the activated sludge under anaerobic conditions which forms a mixture of a liquid rich in soluble phosphorus and an activated sludge low in phosphorus. The sludge and liquid are separated. A portion of the liquid is subjected to solid-liquid separation to form a liquid high in phosphorus and a sludge low in phosphorus. This latter sludge is, along with the other portion of the liquid and the sludge from which the liquid has been initially separated are mixed with oxygen, in the presence of nitric acid or nitrous acid and subjected to aerobic biological oxidation. This results in the formation of a liquid low in phosphorus and an activated sludge. The activated sludge is used as the activated sludge free of oxygen, nitric acid and nitrous acid in the first stage of the process. The liquid high in phosphorus from the portion subjected to solid-liquid separation of the separated liquid is subjected to chemical removal of the soluble phosphorus therein.
摘要翻译：通过将有机废液与基本上不含氧，硝酸和亚硝酸的活性污泥混合，并将该混合物在厌氧条件下进行活性污泥的作用，从含有其的有机废液中除去磷，氮和BOD，形成富含可溶性磷的液体和磷含量低的活性污泥的混合物。污泥和液体分离。将一部分液体进行固液分离以形成高磷的液体和低磷的污泥。在硝酸或亚硝酸的存在下，后一种污泥与液体的最初分离液体和其中最初分离液体的污泥一起与氧气混合并进行有氧生物氧化。这导致磷和活性污泥中液体低的形成。在该过程的第一阶段，活性污泥用作无氧，硝酸和亚硝酸的活性污泥。来自分离液体的固液分离部分的高磷的液体经受化学除去其中的可溶性磷。

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