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1. 发明授权

US6030817A Production of non-native bacterial exopolysaccharide in a recombinant bacterial host 失效
标题翻译：在重组细菌宿主中生产非天然细菌外多糖
公开(公告)号：US6030817A
公开(公告)日：2000-02-29
申请号：US96867
申请日：1998-06-11
申请人： Thomas J. Pollock , Marcia Mikolajczak , Motohide Yamazaki , Linda Thorne , Richard W. Armentrout
发明人： Thomas J. Pollock , Marcia Mikolajczak , Motohide Yamazaki , Linda Thorne , Richard W. Armentrout
IPC分类号： C12N15/09 , C08B37/00 , C12N1/21 , C12N15/52 , C12P19/04 , C12P19/06 , C12R1/01 , C12R1/64 , C07H21/04 , C12N1/20 , C12N5/00
CPC分类号： C12P19/04 , C12N15/52 , C12P19/06 , Y10S435/91
摘要： A new recombinant bacteria for the production of exopolysaccharides is disclosed as well as a method for making the recombinant bacteria and making an exopolysaccharide from the bacteria by submerged aerobic fermentation of the bacteria utilizing a sugar substrate. The exopolysaccharides obtained from the inventive bacteria exhibit improved, more desirable or different properties from the exopolysaccharide produced by the non-recombinant bacteria from which the recombinant bacteria was derived. In addition, the present invention provides a method of producing bacterial exopolysaccharides by fermentation from sugar substrates that the bacteria which the exopolysaccharides are native to cannot utilize.
摘要翻译：公开了一种用于生产胞外多糖的新的重组细菌，以及通过使用糖基质的细菌的潜在好氧发酵来制备重组细菌并从细菌制备外多糖的方法。从本发明细菌获得的外多糖从由重组细菌得到的非重组细菌产生的外多糖表现出改进的，更理想的或不同的性质。此外，本发明提供了通过从糖底物的发酵生产细胞外多糖的方法，所述外多糖本身不能利用的细菌。

2. 发明授权

US6027925A Production of non-native bacterial exopolysaccharide in a recombinant bacterial host 失效
标题翻译：在重组细菌宿主中生产非天然细菌外多糖
公开(公告)号：US6027925A
公开(公告)日：2000-02-22
申请号：US96942
申请日：1998-06-12
申请人： Thomas J. Pollock , Marcia Mikolajczak , Motohide Yamazaki , Linda Thorne , Richard W. Armentrout
发明人： Thomas J. Pollock , Marcia Mikolajczak , Motohide Yamazaki , Linda Thorne , Richard W. Armentrout
IPC分类号： C12N15/09 , C08B37/00 , C12N1/21 , C12N15/52 , C12P19/04 , C12P19/06 , C12R1/01 , C12R1/64 , C07H21/04 , C12N1/20 , C12N5/00
CPC分类号： C12P19/06 , C12N15/52 , C12P19/04 , Y10S435/91
摘要： A recombinant bacteria for the production of exopolysaccharides is disclosed as well as a method for making the recombinant bacteria and making an exopolysaccharide from the bacteria by submerged aerobic fermentation of the bacteria utilizing a sugar substrate. In addition, the present invention provides a method of producing bacterial exopolysaccharides by fermentation from sugar substrates that the wild-type bacteria for producing the exopolysaccharide cannot utilize.
摘要翻译：公开了一种用于制备胞外多糖的重组细菌，以及通过使用糖基质的细菌的潜在好氧发酵来制备重组细菌并从细菌制备外多糖的方法。此外，本发明提供了通过发酵从糖底物生产细胞外多糖的方法，用于产生外多糖的野生型细菌不能利用。

3. 发明授权

US5985623A DNA segments and methods for increasing polysaccharide production 失效
标题翻译： DNA片段和增加多糖生产的方法
公开(公告)号：US5985623A
公开(公告)日：1999-11-16
申请号：US377440
申请日：1995-01-24
申请人： Thomas J. Pollock , Motohide Yamazaki , Linda Thorne , Marcia Mikolajczak , Richard W. Armentrout
发明人： Thomas J. Pollock , Motohide Yamazaki , Linda Thorne , Marcia Mikolajczak , Richard W. Armentrout
IPC分类号： C12N15/52 , C12P19/04 , C07H21/04 , C12N1/20
CPC分类号： C12P19/04 , C12N15/52 , Y02P20/582 , Y10S435/822
摘要： The present invention relates to DNA segments isolated from Sphingomonas sp. and involved in the biosynthetic production of sphingan polysaccharides to increase the production of the polysaccharide in engineered microorganisms. The present invention also relates to methods of engineering strains of Sphingomonas to produce bacteria which are hyperproducers of sphingan, methods of identifying and utilizing DNA fragments useful to enhance production of sphingan in bacteria and the hyperproducer bacteria.
摘要翻译：本发明涉及从鞘氨醇单胞菌属（Sphingomonas sp。）分离的DNA片段。并参与了鞘氨醇多糖的生物合成生产，以增加工程微生物中多糖的产生。本发明还涉及施用鞘氨醇单胞菌菌株以产生鞘氨醇过量产生菌的方法，鉴定和利用可用于增强细菌中的鞘氨醇生产的DNA片段和超生产菌的方法。

4. 发明授权

US06284516B1 DNA segments and methods for increasing polysaccharide production 有权
标题翻译： DNA片段和增加多糖生产的方法
公开(公告)号：US06284516B1
公开(公告)日：2001-09-04
申请号：US09440530
申请日：1999-11-15
申请人： Thomas J. Pollock , Motohide Yamazaki , Linda Thorne , Marcia Mikolajczak , Richard W. Armentrout
发明人： Thomas J. Pollock , Motohide Yamazaki , Linda Thorne , Marcia Mikolajczak , Richard W. Armentrout
IPC分类号： C12N120
CPC分类号： C12P19/04 , C12N15/52 , Y02P20/582 , Y10S435/822
摘要： The present invention relates to DNA segments isolated from Sphingomonas sp. and involved in the biosynthetic production of sphingan polysaccharides to increase the production of the polysaccharide in engineered microorganisms. The present invention also relates to methods of engineering strains of Sphingomonas to produce bacteria which are hyperproducers of sphingan, methods of identifying and utilizing DNA fragments useful to enhance production of sphingan in bacteria and the hyperproducer bacteria.
摘要翻译：本发明涉及从鞘氨醇单胞菌属（Sphingomonas sp。）分离的DNA片段。并参与了鞘氨醇多糖的生物合成生产，以增加工程微生物中多糖的产生。本发明还涉及施用鞘氨醇单胞菌菌株以产生鞘氨醇过量产生菌的方法，鉴定和利用可用于增强细菌中的鞘氨醇生产的DNA片段和超生产菌的方法。

5. 发明授权

US5854034A DNA segments and methods for increasing polysaccharide production 失效
公开(公告)号：US5854034A
公开(公告)日：1998-12-29
申请号：US592874
申请日：1996-01-24
申请人： Thomas J. Pollock , Motohide Yamazaki , Linda Thorne , Marcia Mikolajczak , Richard W. Armentrout
发明人： Thomas J. Pollock , Motohide Yamazaki , Linda Thorne , Marcia Mikolajczak , Richard W. Armentrout
IPC分类号： C12N15/52 , C12P19/04 , C12N1/21 , C12P19/06 , C12P21/04
CPC分类号： C12P19/04 , C12N15/52 , Y02P20/582 , Y10S435/822
摘要： The present invention relates to DNA segments isolated from Sphingomonas sp. and involved in the biosynthetic production of sphingan polysaccharides to increase the production of the polysaccharide in engineered microorganisms. The present invention also relates to methods of engineering strains of Sphingomonas to produce bacteria which are hyperproducers of sphingan, methods of identifying and utilizing DNA fragments useful to enhance production of sphingan in bacteria and the hyperproducer bacteria.

6. 发明授权

US06605461B2 Production of exopolysaccharides unattached to the surface of bacterial cells 失效
公开(公告)号：US06605461B2
公开(公告)日：2003-08-12
申请号：US09905829
申请日：2001-07-13
申请人： Motohide Yamazaki , Marcia Mikolajczak , Thomas J. Pollock , Richard W. Armentrout
发明人： Motohide Yamazaki , Marcia Mikolajczak , Thomas J. Pollock , Richard W. Armentrout
IPC分类号： C12N120
CPC分类号： C12R1/01 , C08B37/00 , C08B37/006 , C12P19/04
摘要： The present invention is directed to a Sphingomonas bacteria and a method of producing exopolysaccharides by culturing a Shingomonas bacteria in a fermentation broth for a time and temperature effective for providing a sphingan exopolysaccharide in a slime form.

7. 发明授权

US07390644B2 Production of exopolysaccharides unattached to the surface of bacterial cells 失效
标题翻译：生产不附着于细菌细胞表面的外多糖
公开(公告)号：US07390644B2
公开(公告)日：2008-06-24
申请号：US10617371
申请日：2003-07-10
申请人： Motohide Yamazaki , Marcia Mikolajczak , Thomas J. Pollock , Richard W. Armentrout
发明人： Motohide Yamazaki , Marcia Mikolajczak , Thomas J. Pollock , Richard W. Armentrout
IPC分类号： C08B37/00 , C12N1/20 , C12N1/12 , C12P17/02 , C12P19/04 , C12P1/04
CPC分类号： C12R1/01 , C08B37/00 , C08B37/006 , C12P19/04
摘要： The present invention is directed to a Sphingomonas bacteria and a method of producing exopolysaccharides by culturing a Shpingomonas bacteria in a fermentation broth for a time and temperature effective for providing a sphingan exopolysaccharide in a slime form.
摘要翻译：本发明涉及一种鞘氨醇单胞菌和通过在发酵液中培养沙门氏菌属细菌的时间和温度来生产胞外多糖的方法，所述时间和温度有效地提供了一种以泥状形式的鞘氨醇胞外多糖。

8. 发明授权

US5190866A Process for producing antibiotic streptovaricin 失效
标题翻译：生产抗生素链脲霉素的方法
公开(公告)号：US5190866A
公开(公告)日：1993-03-02
申请号：US826371
申请日：1992-01-27
申请人： Kaname Inoue , Motohide Yamazaki , Richard W. Armentrout
发明人： Kaname Inoue , Motohide Yamazaki , Richard W. Armentrout
IPC分类号： C12N1/02 , C12P1/00 , C12P17/18
CPC分类号： C12P17/18 , C12N1/02 , C12P1/00 , Y10S435/803 , Y10S435/886
摘要： A method for separation and recovery of polymeric beads from an antibiotic fermentation broth comprising suspending a mixture of the beads and any inherent mold from the fermentation in an aqueous solution having a specific gravity which is effective to cause the beads to form a discrete layer at or on the surface of the solution, separate and apart from the mold. The separate layer of the beads may then be easily removed from the liquid by a conventional physical methods.
摘要翻译：一种用于从抗生素发酵液中分离和回收聚合物珠的方法，其包括将珠粒和任何固有的霉菌的混合物从发酵中悬浮在具有比重的水溶液中，该水溶液有效地使珠粒在或在溶液的表面上，与模具分离并分开。然后可以通过常规的物理方法将珠的单独层容易地从液体中除去。

9. 发明授权

US5258291A Process for producing antibiotics 失效
标题翻译：生产抗生素的方法
公开(公告)号：US5258291A
公开(公告)日：1993-11-02
申请号：US17495
申请日：1993-02-12
申请人： Kaname Inoue , Motohide Yamazaki , Richard W. Armentrout
发明人： Kaname Inoue , Motohide Yamazaki , Richard W. Armentrout
IPC分类号： C12N1/02 , C12P1/00 , C12P17/18 , C12N1/36 , C12P17/08 , C12R1/465
CPC分类号： C12P1/00 , C12N1/02 , C12P17/18 , Y10S435/803 , Y10S435/886
摘要： A method for separation and recovery of polymeric beads from an antibiotic fermentation broth comprising suspending a mixture of the beads and any inherent mold from the fermentation in an aqueous solution having a specific gravity which is effective to cause the beads to form a discrete layer at or on the surface of the solution, separate and apart from the mold. The separate layer of the beads may then be easily removed from the liquid by a conventional physical methods.
摘要翻译：一种用于从抗生素发酵液中分离和回收聚合物珠的方法，其包括将珠粒和任何固有的霉菌的混合物从发酵中悬浮在具有比重的水溶液中，该水溶液有效地使珠粒在或在溶液的表面上，与模具分离并分开。然后可以通过常规的物理方法将珠的单独层容易地从液体中除去。

10. 发明授权

US5210033A Process for preparing and selecting hyperproducing microorganism strains used for the process for producing streptovaricin C 失效
标题翻译：制备和选择用于生产链脲霉素C的方法的超生产微生物菌株的方法
公开(公告)号：US5210033A
公开(公告)日：1993-05-11
申请号：US872519
申请日：1992-04-23
申请人： Kaname Inoue , Motohide Yamazaki , Kanji Murofushi , Richard W. Armentrout
发明人： Kaname Inoue , Motohide Yamazaki , Kanji Murofushi , Richard W. Armentrout
IPC分类号： C12P17/18
CPC分类号： C12R1/465 , C12P17/18 , Y10S435/803 , Y10S435/886
摘要： Disclosed is a method for selecting a mutant strain belonging to the genus Streptomyces which is a hyper-producer of Streptovaricin C superior to those know heretofore. This is accomplished by first subjecting a natural strain of Streptomyces spectabilis to conditions so as to isolate organisms which are streptovaricin resistant. The streptovaricin resistant organisms thus isolated are then subjected to mutagenesis and then cultured. The colonies which are asporogenous are individually cultured in fermentation batches such that the strains take the form of pellets of varying sizes and colors. From the batch having the most heterogeneous mixture of pellets, the smallest pellet or the pellet(s) having the deepest color (usually deep red or crimson) is isolated. We have discovered that the strain of this pellet has a high likelihood of being a hyperproducer of streptovaricin. The strain of this pellet may then be subjected to fermentation conditions to produce streptovaricin. The nutrient broth containing fumaric acid or water-soluble salts thereof, and adsorbent polymer beads and the streptovaricin produced are recovered in the usual manner. In another embodiment, the fermentation may be carried out in a nutrient broth wherein fumaric acid or its water-soluble salts are deliberately excluded to achieve even higher productivity.
摘要翻译：公开了一种选择属于链霉菌属（Streptomyces）属的突变菌株的方法，其是链节链球菌C的超级生产者，其比迄今所知道的更高。这是通过首先对天然链霉菌链霉菌进行条件来分离的，以分离链霉素抗性的生物体。然后将如此分离的链脲霉素抗性生物体进行诱变，然后进行培养。在发酵批次中单独培养异源菌落，使得菌株呈不同大小和颜色的颗粒的形式。从具有最不均匀的颗粒混合物的批料中，分离出具有最深颜色（通常深红色或深红色）的最小颗粒或颗粒。我们已经发现，这种颗粒的菌株很可能成为链球菌素的超生产菌。然后可以将该颗粒的菌株进行发酵条件以产生链脲霉素。以通常的方式回收含有富马酸或其水溶性盐的营养液，以及生成的吸附性聚合物珠粒和链球菌素。在另一个实施方案中，发酵可以在营养肉汤中进行，其中富马酸或其水溶性盐被有意排除以达到更高的生产率。

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