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1. 发明授权

US06284516B1 DNA segments and methods for increasing polysaccharide production 有权
标题翻译： DNA片段和增加多糖生产的方法
公开(公告)号：US06284516B1
公开(公告)日：2001-09-04
申请号：US09440530
申请日：1999-11-15
申请人： Thomas J. Pollock , Motohide Yamazaki , Linda Thorne , Marcia Mikolajczak , Richard W. Armentrout
发明人： Thomas J. Pollock , Motohide Yamazaki , Linda Thorne , Marcia Mikolajczak , Richard W. Armentrout
IPC分类号： C12N120
CPC分类号： C12P19/04 , C12N15/52 , Y02P20/582 , Y10S435/822
摘要： The present invention relates to DNA segments isolated from Sphingomonas sp. and involved in the biosynthetic production of sphingan polysaccharides to increase the production of the polysaccharide in engineered microorganisms. The present invention also relates to methods of engineering strains of Sphingomonas to produce bacteria which are hyperproducers of sphingan, methods of identifying and utilizing DNA fragments useful to enhance production of sphingan in bacteria and the hyperproducer bacteria.
摘要翻译：本发明涉及从鞘氨醇单胞菌属（Sphingomonas sp。）分离的DNA片段。并参与了鞘氨醇多糖的生物合成生产，以增加工程微生物中多糖的产生。本发明还涉及施用鞘氨醇单胞菌菌株以产生鞘氨醇过量产生菌的方法，鉴定和利用可用于增强细菌中的鞘氨醇生产的DNA片段和超生产菌的方法。

2. 发明授权

US06605461B2 Production of exopolysaccharides unattached to the surface of bacterial cells 失效
公开(公告)号：US06605461B2
公开(公告)日：2003-08-12
申请号：US09905829
申请日：2001-07-13
申请人： Motohide Yamazaki , Marcia Mikolajczak , Thomas J. Pollock , Richard W. Armentrout
发明人： Motohide Yamazaki , Marcia Mikolajczak , Thomas J. Pollock , Richard W. Armentrout
IPC分类号： C12N120
CPC分类号： C12R1/01 , C08B37/00 , C08B37/006 , C12P19/04
摘要： The present invention is directed to a Sphingomonas bacteria and a method of producing exopolysaccharides by culturing a Shingomonas bacteria in a fermentation broth for a time and temperature effective for providing a sphingan exopolysaccharide in a slime form.

3. 发明授权

US6030817A Production of non-native bacterial exopolysaccharide in a recombinant bacterial host 失效
标题翻译：在重组细菌宿主中生产非天然细菌外多糖
公开(公告)号：US6030817A
公开(公告)日：2000-02-29
申请号：US96867
申请日：1998-06-11
申请人： Thomas J. Pollock , Marcia Mikolajczak , Motohide Yamazaki , Linda Thorne , Richard W. Armentrout
发明人： Thomas J. Pollock , Marcia Mikolajczak , Motohide Yamazaki , Linda Thorne , Richard W. Armentrout
IPC分类号： C12N15/09 , C08B37/00 , C12N1/21 , C12N15/52 , C12P19/04 , C12P19/06 , C12R1/01 , C12R1/64 , C07H21/04 , C12N1/20 , C12N5/00
CPC分类号： C12P19/04 , C12N15/52 , C12P19/06 , Y10S435/91
摘要： A new recombinant bacteria for the production of exopolysaccharides is disclosed as well as a method for making the recombinant bacteria and making an exopolysaccharide from the bacteria by submerged aerobic fermentation of the bacteria utilizing a sugar substrate. The exopolysaccharides obtained from the inventive bacteria exhibit improved, more desirable or different properties from the exopolysaccharide produced by the non-recombinant bacteria from which the recombinant bacteria was derived. In addition, the present invention provides a method of producing bacterial exopolysaccharides by fermentation from sugar substrates that the bacteria which the exopolysaccharides are native to cannot utilize.
摘要翻译：公开了一种用于生产胞外多糖的新的重组细菌，以及通过使用糖基质的细菌的潜在好氧发酵来制备重组细菌并从细菌制备外多糖的方法。从本发明细菌获得的外多糖从由重组细菌得到的非重组细菌产生的外多糖表现出改进的，更理想的或不同的性质。此外，本发明提供了通过从糖底物的发酵生产细胞外多糖的方法，所述外多糖本身不能利用的细菌。

4. 发明授权

US6027925A Production of non-native bacterial exopolysaccharide in a recombinant bacterial host 失效
标题翻译：在重组细菌宿主中生产非天然细菌外多糖
公开(公告)号：US6027925A
公开(公告)日：2000-02-22
申请号：US96942
申请日：1998-06-12
申请人： Thomas J. Pollock , Marcia Mikolajczak , Motohide Yamazaki , Linda Thorne , Richard W. Armentrout
发明人： Thomas J. Pollock , Marcia Mikolajczak , Motohide Yamazaki , Linda Thorne , Richard W. Armentrout
IPC分类号： C12N15/09 , C08B37/00 , C12N1/21 , C12N15/52 , C12P19/04 , C12P19/06 , C12R1/01 , C12R1/64 , C07H21/04 , C12N1/20 , C12N5/00
CPC分类号： C12P19/06 , C12N15/52 , C12P19/04 , Y10S435/91
摘要： A recombinant bacteria for the production of exopolysaccharides is disclosed as well as a method for making the recombinant bacteria and making an exopolysaccharide from the bacteria by submerged aerobic fermentation of the bacteria utilizing a sugar substrate. In addition, the present invention provides a method of producing bacterial exopolysaccharides by fermentation from sugar substrates that the wild-type bacteria for producing the exopolysaccharide cannot utilize.
摘要翻译：公开了一种用于制备胞外多糖的重组细菌，以及通过使用糖基质的细菌的潜在好氧发酵来制备重组细菌并从细菌制备外多糖的方法。此外，本发明提供了通过发酵从糖底物生产细胞外多糖的方法，用于产生外多糖的野生型细菌不能利用。

5. 发明授权

US5985623A DNA segments and methods for increasing polysaccharide production 失效
标题翻译： DNA片段和增加多糖生产的方法
公开(公告)号：US5985623A
公开(公告)日：1999-11-16
申请号：US377440
申请日：1995-01-24
申请人： Thomas J. Pollock , Motohide Yamazaki , Linda Thorne , Marcia Mikolajczak , Richard W. Armentrout
发明人： Thomas J. Pollock , Motohide Yamazaki , Linda Thorne , Marcia Mikolajczak , Richard W. Armentrout
IPC分类号： C12N15/52 , C12P19/04 , C07H21/04 , C12N1/20
CPC分类号： C12P19/04 , C12N15/52 , Y02P20/582 , Y10S435/822
摘要： The present invention relates to DNA segments isolated from Sphingomonas sp. and involved in the biosynthetic production of sphingan polysaccharides to increase the production of the polysaccharide in engineered microorganisms. The present invention also relates to methods of engineering strains of Sphingomonas to produce bacteria which are hyperproducers of sphingan, methods of identifying and utilizing DNA fragments useful to enhance production of sphingan in bacteria and the hyperproducer bacteria.
摘要翻译：本发明涉及从鞘氨醇单胞菌属（Sphingomonas sp。）分离的DNA片段。并参与了鞘氨醇多糖的生物合成生产，以增加工程微生物中多糖的产生。本发明还涉及施用鞘氨醇单胞菌菌株以产生鞘氨醇过量产生菌的方法，鉴定和利用可用于增强细菌中的鞘氨醇生产的DNA片段和超生产菌的方法。

6. 发明授权

US07390644B2 Production of exopolysaccharides unattached to the surface of bacterial cells 失效
标题翻译：生产不附着于细菌细胞表面的外多糖
公开(公告)号：US07390644B2
公开(公告)日：2008-06-24
申请号：US10617371
申请日：2003-07-10
申请人： Motohide Yamazaki , Marcia Mikolajczak , Thomas J. Pollock , Richard W. Armentrout
发明人： Motohide Yamazaki , Marcia Mikolajczak , Thomas J. Pollock , Richard W. Armentrout
IPC分类号： C08B37/00 , C12N1/20 , C12N1/12 , C12P17/02 , C12P19/04 , C12P1/04
CPC分类号： C12R1/01 , C08B37/00 , C08B37/006 , C12P19/04
摘要： The present invention is directed to a Sphingomonas bacteria and a method of producing exopolysaccharides by culturing a Shpingomonas bacteria in a fermentation broth for a time and temperature effective for providing a sphingan exopolysaccharide in a slime form.
摘要翻译：本发明涉及一种鞘氨醇单胞菌和通过在发酵液中培养沙门氏菌属细菌的时间和温度来生产胞外多糖的方法，所述时间和温度有效地提供了一种以泥状形式的鞘氨醇胞外多糖。

7. 发明授权

US5854034A DNA segments and methods for increasing polysaccharide production 失效
公开(公告)号：US5854034A
公开(公告)日：1998-12-29
申请号：US592874
申请日：1996-01-24
申请人： Thomas J. Pollock , Motohide Yamazaki , Linda Thorne , Marcia Mikolajczak , Richard W. Armentrout
发明人： Thomas J. Pollock , Motohide Yamazaki , Linda Thorne , Marcia Mikolajczak , Richard W. Armentrout
IPC分类号： C12N15/52 , C12P19/04 , C12N1/21 , C12P19/06 , C12P21/04
CPC分类号： C12P19/04 , C12N15/52 , Y02P20/582 , Y10S435/822
摘要： The present invention relates to DNA segments isolated from Sphingomonas sp. and involved in the biosynthetic production of sphingan polysaccharides to increase the production of the polysaccharide in engineered microorganisms. The present invention also relates to methods of engineering strains of Sphingomonas to produce bacteria which are hyperproducers of sphingan, methods of identifying and utilizing DNA fragments useful to enhance production of sphingan in bacteria and the hyperproducer bacteria.

8. 发明授权

US06602997B2 Whole cell and cell-debris polysaccharide 失效
标题翻译：全细胞和细胞碎片多糖
公开(公告)号：US06602997B2
公开(公告)日：2003-08-05
申请号：US09838650
申请日：2001-04-19
申请人： Marcia Mikolajczak , Motohide Yamazaki , Thomas J. Pollock
发明人： Marcia Mikolajczak , Motohide Yamazaki , Thomas J. Pollock
IPC分类号： C08B3700
CPC分类号： C08B37/0003 , C08B37/006 , C12P19/04
摘要： The present invention provides for a method for obtaining a polysaccharide substantially free from whole bacterial cells and bacterial cell debris including: (a) fermenting a Sphingomonas bacterium, which produces the polysaccharide, to produce an aqueous fermentation broth having the polysaccharide dissolved therein; (b) diluting the fermentation broth with an equal volume of deionized water; (c) partially hydrolyzing the polysaccharide by exposing the fermentation broth resulting from step (b) to a temperature in excess of 100° C. for a time period from about ten minutes to about one hour; (d) removing bacterial cells from the polysaccharide by centrifugation of the fermentation broth and recovery of a supernatant aqueous liquid; (e) precipitating the polysaccharide from the supernatant aqueous liquid by adding a second liquid to the supernatant aqueous liquid, wherein the second liquid is miscible with water, non-reactive with the polysaccharide, and in which the polysaccharide is substantially insoluble; and (f) harvesting the polysaccharide resulting from step (e) by separating it from the supernatant aqueous liquid.
摘要翻译：本发明提供一种获得基本上不含整个细菌细菌和细菌细胞碎片的多糖的方法，包括：（a）发酵产生多糖的鞘氨醇单胞菌，产生其中溶解有多糖的水性发酵液;（b ）用等体积的去离子水稀释发酵肉汤;（c）通过将由步骤（b）得到的发酵液暴露于超过100℃的温度下将部分水解约10分钟至约1小时;（d）通过离心发酵肉汤并回收上清液含水液体从多糖中除去细菌细胞;（e）通过向上清液含水液体中加入第二液体而使上清液含水液体中的多糖沉淀，其中第二液体与水混溶，与多糖不反应，多糖是其中的抗溶性; 和（f）从上清液含水液体中分离出步骤（e）得到的多糖。

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