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1. 发明授权

US5916428A Automated system for multi-capillary electrophoresis having a two-dimensional array of capillary ends 失效
标题翻译：具有毛细管端的二维阵列的多毛细管电泳的自动系统
公开(公告)号：US5916428A
公开(公告)日：1999-06-29
申请号：US110877
申请日：1998-07-07
申请人： Thomas E. Kane , Qingbo China Li , John R. Kernan
发明人： Thomas E. Kane , Qingbo China Li , John R. Kernan
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44704 , G01N27/44782
摘要： An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The capillary cartridge has two embodiments. In one embodiment, the second ends of the capillary tubes are also arranged is such an array. In a second embodiment, the second ends communicate with an interior cavity of pressure cell from which solutions, gels and the like may be introduced. The pressure cell allows for applying high pressure to clean out the capillary tubes. An apparatus for performing automated capillary gel electrophoresis using such a cartridge is also disclosed.
摘要翻译：公开了一种自动电泳系统。该系统采用具有多个毛细管的毛细管盒。盒具有从板的一侧突出的第一阵列的毛细管端。毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。毛细管盒具有两个实施例。在一个实施例中，毛细管的第二端也布置为这样的阵列。在第二实施例中，第二端与压力池的内部空腔连通，可以引入溶液，凝胶等。压力池允许施加高压以清洁毛细管。还公开了使用这种盒进行自动毛细管凝胶电泳的装置。

2. 发明授权

US06375819B1 Fluid delivery system for capillary electrophoretic applications 失效
标题翻译：用于毛细管电泳应用的流体输送系统
公开(公告)号：US06375819B1
公开(公告)日：2002-04-23
申请号：US09373909
申请日：1999-08-13
申请人： Qingbo Li , Changsheng Liu , Thomas E. Kane , John R. Kernan , Bernard Sonnenschein , Michael V. Sharer
发明人： Qingbo Li , Changsheng Liu , Thomas E. Kane , John R. Kernan , Bernard Sonnenschein , Michael V. Sharer
IPC分类号： G01N2726
CPC分类号： G01N27/44721 , G01N27/44782
摘要： An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.
摘要翻译：公开了一种自动电泳系统。该系统采用具有多个毛细管的毛细管盒。盒具有从板的一侧突出的第一阵列的毛细管端。毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。该系统还具有含有凝胶注射器/步进电机或具有泵的高压室的凝胶递送模块，以通过毛细管快速均匀地输送凝胶。该系统还包括多波长光束发生器，以产生产生具有宽波长范围的光束的激光束。离线毛细管修复器彻底清洁毛细管柱，以便同时执行另一个毛细管柱的电泳。离线毛细管再生器的流线型性质提供了增加系统吞吐量的优点，同时最小化了系统成本。

3. 发明授权

US5885430A Capillary tube holder for an electrophoretic apparatus 失效
标题翻译：用于电泳设备的毛细管支架
公开(公告)号：US5885430A
公开(公告)日：1999-03-23
申请号：US726877
申请日：1996-10-04
申请人： John R. Kernan , Thomas E. Kane
发明人： John R. Kernan , Thomas E. Kane
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44704 , G01N27/44782
摘要： An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The capillary cartridge has two embodiments. In one embodiment, the second ends of the capillary tubes are also arranged is such an array. In a second embodiment, the second ends communicate with an interior cavity of pressure cell from which solutions, gels and the like may be introduced. The pressure cell allows for applying high pressure to clean out the capillary tubes. An apparatus for performing automated capillary gel electrophoresis using such a cartridge is also disclosed.
摘要翻译：公开了一种自动电泳系统。该系统采用具有多个毛细管的毛细管盒。盒具有从板的一侧突出的第一阵列的毛细管端。毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。毛细管盒具有两个实施例。在一个实施例中，毛细管的第二端也布置为这样的阵列。在第二实施例中，第二端与压力池的内部空腔连通，可以引入溶液，凝胶等。压力池允许施加高压以清洁毛细管。还公开了使用这种盒进行自动毛细管凝胶电泳的装置。

4. 发明授权

US6063251A Electrically insulated capillary arrays for electrophoretic applications 失效
标题翻译：用于电泳应用的电绝缘毛细管阵列
公开(公告)号：US6063251A
公开(公告)日：2000-05-16
申请号：US866308
申请日：1997-05-30
申请人： Thomas E. Kane , John R. Kernan , Changsheng Liu , Qingbo Li
发明人： Thomas E. Kane , John R. Kernan , Changsheng Liu , Qingbo Li
IPC分类号： G01N27/447
CPC分类号： G01N27/44782
摘要： An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. Electrical crosstalk between neighboring capillaries is attenuated using a protective tubing formed from an electrically insulative material. This crosstalk attenuation is provided in the form of sheathing, either around individual capillaries, or around bundles of spaced apart capillaries. Crosstalk attenuation may be enhanced by passing a nonconductive gas or liquid through lumens formed in the protective tubing, in which lumens the capillary tubes are present.
摘要翻译：公开了一种自动电泳系统。该系统采用具有多个毛细管的毛细管盒。盒具有从板的一侧突出的第一阵列的毛细管端。毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。使用由电绝缘材料形成的保护管，相邻毛细管之间的电气串扰被衰减。这种串扰衰减以围绕单个毛细管或围绕分开的毛细管束的护套形式提供。可以通过使非导电气体或液体通过在保护管中形成的内腔（其中存在毛细管的管腔）来增强串扰衰减。

5. 发明授权

US6027627A Automated parallel capillary electrophoretic system 失效
标题翻译：自动并行毛细管电泳系统
公开(公告)号：US6027627A
公开(公告)日：2000-02-22
申请号：US105988
申请日：1998-06-29
申请人： Qingbo Li , Thomas E. Kane , Changsheng Liu , Bernard Sonnenschein , Michael V. Sharer , John R. Kernan
发明人： Qingbo Li , Thomas E. Kane , Changsheng Liu , Bernard Sonnenschein , Michael V. Sharer , John R. Kernan
IPC分类号： G01N27/447 , G01N27/26 , G01N27/477
CPC分类号： G01N27/44782
摘要： An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.
摘要翻译：公开了一种自动电泳系统。该系统采用具有多个毛细管的毛细管盒。盒具有从板的一侧突出的第一阵列的毛细管端。毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。该系统还具有含有凝胶注射器/步进电机或具有泵的高压室的凝胶递送模块，以通过毛细管快速均匀地输送凝胶。该系统还包括多波长光束发生器，以产生产生具有宽波长范围的光束的激光束。离线毛细管修复器彻底清洁毛细管柱，以便同时执行另一个毛细管柱的电泳。离线毛细管再生器的流线型性质提供了增加系统吞吐量的优点，同时最小化了系统成本。

6. 发明授权

US06365024B1 Motorized positioning apparatus having coaxial carrousels 有权
标题翻译：具有同轴转盘的电动定位装置
公开(公告)号：US06365024B1
公开(公告)日：2002-04-02
申请号：US09372151
申请日：1999-08-11
申请人： Qingbo Li , Thomas E. Kane , Changsheng Liu , Bernard Sonnenschein , Michael V. Sharer , John R. Kernan
发明人： Qingbo Li , Thomas E. Kane , Changsheng Liu , Bernard Sonnenschein , Michael V. Sharer , John R. Kernan
IPC分类号： G01N2726
CPC分类号： G01N27/44721 , G01N27/44782
摘要： An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.
摘要翻译：公开了一种自动电泳系统。该系统采用具有多个毛细管的毛细管盒。盒具有从板的一侧突出的第一阵列的毛细管端。毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。该系统还具有含有凝胶注射器/步进电机或具有泵的高压室的凝胶递送模块，以通过毛细管快速均匀地输送凝胶。该系统还包括多波长光束发生器，以产生产生具有宽波长范围的光束的激光束。离线毛细管修复器彻底清洁毛细管柱，以便同时执行另一个毛细管柱的电泳。离线毛细管再生器的流线型性质提供了增加系统吞吐量的优点，同时最小化了系统成本。

7. 发明授权

US06982029B2 Electrophoretic method and system having internal lane standards for color calibration 有权
公开(公告)号：US06982029B2
公开(公告)日：2006-01-03
申请号：US09849239
申请日：2001-05-07
申请人： Walter R. McVey , Thomas E. Kane
发明人： Walter R. McVey , Thomas E. Kane
IPC分类号： B01D59/42 , C07K1/26
CPC分类号： G01N27/44721 , G01N27/44726
摘要： An electrophoretic system having a plurality of separation lanes is provided with an automatic color calibration feature in which each lane is separately calibrated. For each lane, a dye matrix standard is created using reference fragments which migrate either before, or after, the sample fragments being electrophoresced, during the same electrophoresis run. These dye standards are detected automatically for the purpose of determining coefficients used to identify sample fragments mixed together with the reference fragments. This allows for calibrating the dye standard matrix for each of a plurality of lanes each time the electrophoresis system is used.

8. 发明申请

US20100282077A1 Gas chromatography inlet liner having at least one indicator 有权
标题翻译：具有至少一个指示剂的气相色谱入口衬套
公开(公告)号：US20100282077A1
公开(公告)日：2010-11-11
申请号：US12660241
申请日：2010-02-23
申请人： Brian A. Jones , Thomas E. Kane
发明人： Brian A. Jones , Thomas E. Kane
IPC分类号： B44F1/08 , B01D53/02
CPC分类号： G01N30/12
摘要： A gas chromatography inlet liner comprises a glass tube formed by a glass wall, the glass wall having pore channels formed therein, and an indicator present in the pore channels in at least part of the inlet liner. The indicator may be any element, compound, dopant, or mixture additive that modifies color or transparency of the inlet liner.
摘要翻译：气相色谱入口衬套包括由玻璃壁形成的玻璃管，其中形成有孔隙通道的玻璃壁和存在于入口衬里的至少一部分中的孔通道中的指示器。指示剂可以是改变入口衬里的颜色或透明度的任何元素，化合物，掺杂剂或混合物添加剂。

9. 发明申请

US20090090630A1 Automated parallel capillary electrophoresis system with hydrodynamic sample injection 审中-公开
标题翻译：自动平行毛细管电泳系统与流体动力学样品注射
公开(公告)号：US20090090630A1
公开(公告)日：2009-04-09
申请号：US12290087
申请日：2008-10-27
申请人： Changsheng Liu , Thomas E. Kane , Qingbo Li
发明人： Changsheng Liu , Thomas E. Kane , Qingbo Li
IPC分类号： G01N27/453
CPC分类号： C07K1/26 , G01N27/44704 , G01N27/44743 , G01N27/44782 , Y10T436/2575
摘要： An automated capillary zone electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends is spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a container connected to the detection end of the capillaries. The container is provided with valving which facilitate cleaning the capillaries, loading buffer into the capillaries, introducing samples to be electrophoresed into the capillaries, and performing capillary zone electrophoresis on the thus introduced samples.
摘要翻译：公开了一种自动化毛细管区域电泳系统。该系统采用具有多个毛细管的毛细管盒。盒具有从板的一侧突出的第一阵列的毛细管端。毛细管端部的第一阵列以与标准尺寸的微量滴定塔的孔基本相同的方式间隔开。这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。该系统还具有连接到毛细管的检测端的容器。该容器设置有阀门，其有助于清洁毛细管，将缓冲液加载到毛细管中，将待电泳的样品引入毛细管中，并在这样引入的样品上进行毛细管区带电泳。

10. 发明授权

US07435545B2 Method for sizing polynucleotides using electrophoresis with non-DNA size standards 有权
标题翻译：使用非DNA大小标准的电泳定型多核苷酸的方法
公开(公告)号：US07435545B2
公开(公告)日：2008-10-14
申请号：US11103356
申请日：2005-04-11
申请人： Zhaowei Liu , Thomas E. Kane
发明人： Zhaowei Liu , Thomas E. Kane
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , C12Q2565/125 , C12Q2565/102 , C12Q2545/101
摘要： According to a method of determining a size of a sample polynucleotide, a sample polynucleotide is subjected to electrophoresis in the presence of a fluorescent compound having a first fluorescence spectrum. Detection of light of the first fluorescence spectrum is indicative of the presence of the sample polynucleotide. One or more size standards are also subjected to electrophoresis, optionally in the presence of the sample polynucleotide. If more than one size standard is used, the different size standards typically have different mobilities. The size standards are generally essentially or completely free of polynucleotides. Migration coordinates, e.g., migration times, of the sample polynucleotide and size standard(s) are determined. A size of the sample polynucleotide can be determined using the migration coordinate of the sample polynucleotide and the migration coordinate(s) of the size standard(s).
摘要翻译：根据测定样品多核苷酸的大小的方法，在具有第一荧光光谱的荧光化合物的存在下对样品多核苷酸进行电泳。第一荧光光谱的光的检测指示样品多核苷酸的存在。也可以在样品多核苷酸的存在下，一个或多个尺寸标准品进行电泳。如果使用多于一个尺寸的标准，则不同尺寸的标准通常具有不同的移动性。大小标准通常基本上或完全不含多核苷酸。测定样品多核苷酸的迁移坐标，例如迁移时间和大小标准。可以使用样品多核苷酸的迁移坐标和尺寸标准的迁移坐标来确定样品多核苷酸的大小。

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