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1. 发明授权

US07595202B2 Analysis method using reporter (label) intermolecular interaction 有权
标题翻译：分析方法采用报告（标记）分子间相互作用
公开(公告)号：US07595202B2
公开(公告)日：2009-09-29
申请号：US10475540
申请日：2002-04-23
申请人： Teruyuki Nagamune , Hiroshi Ueda , Yoshiyuki Ohiro , Norio Shibata
发明人： Teruyuki Nagamune , Hiroshi Ueda , Yoshiyuki Ohiro , Norio Shibata
IPC分类号： G01N33/541 , G01N21/76 , G01N33/533 , G01N33/566 , G01N33/53 , G01N33/542
CPC分类号： G01N33/54306 , G01N33/533 , G01N33/542 , G01N33/582
摘要： The present invention provides a method for controlling the spatial arrangement of an energy donor and an energy acceptor in a reaction complex in order to carry out the measurement with good precision and high sensitivity, and a measurement system using the method. Two types of material having affinity for the subject material to be measured are respectively labeled with a combination of reporters that give rise to energy transfer; those that have been thus obtained by labeling these materials are each further labeled with materials that have weak affinity for each other to give reagents, which are then mixed with a sample to give the reaction complex. Each of the materials is brought into spatial proximity by binding based on the affinity among the materials having weak affinity for each other in the reaction complex, and since that condition is stably maintained, a more efficient energy transfer occurs.
摘要翻译：本发明提供了一种用于控制反应复合物中能量供体和能量受体的空间排列的方法，以便以高精度和高灵敏度进行测量，以及使用该方法的测量系统。分别用引起能量转移的记者的组合来标示对被测量物质具有亲和性的两种类型的材料; 通过标记这些材料得到的那些物质每个进一步用彼此具有弱亲和力的材料进行标记，得到试剂，然后将其与样品混合以得到反应络合物。通过基于在反应络合物中彼此之间具有弱亲和力的材料之间的亲和力，通过结合使每种材料变得空间接近，并且由于稳定地保持该条件，因此发生更有效的能量转移。

2. 发明授权

US06482596B1 Method for measuring antigen concentration 有权
标题翻译：抗原浓度测定方法
公开(公告)号：US06482596B1
公开(公告)日：2002-11-19
申请号：US09313350
申请日：1999-05-18
申请人： Hiroshi Ueda , Teruyuki Nagamune , Hajime Nishimura , Izumi Kumagai , Kouhei Tsumoto , Walter C. Mahoney , Greg Winter , Paula A. Schueler
发明人： Hiroshi Ueda , Teruyuki Nagamune , Hajime Nishimura , Izumi Kumagai , Kouhei Tsumoto , Walter C. Mahoney , Greg Winter , Paula A. Schueler
IPC分类号： G01N3353
CPC分类号： G01N33/6845 , C07K16/18 , G01N33/68 , G01N33/6857
摘要： This invention provides a method for measuring an antigen concentration in a sample, which comprises: preparing VH-domain polypeptide and VL-domain polypeptide of an antibody specific to the antigen; labeling one of the polypeptides with a reporter molecule to form labeled polypeptides, and immobilizing the other polypeptide onto solid-phase to form immobilized polypeptides; contacting the antigen-containing sample and the labeled polypeptides with the solid-phase; and measuring the reporter molecule of the labeled polypeptides bound to the immobilized polypeptides. The present invention permits simpler and quicker sandwich ELISA for measurements of an antigen concentration in high sensitivity.
摘要翻译：本发明提供了一种测定样品中抗原浓度的方法，包括：制备抗原特异性抗体的VH结构域多肽和VL结构域多肽; 用报告分子标记多肽之一以形成标记的多肽，并将另一多肽固定在固相上以形成固定的多肽; 使含抗原的样品和标记的多肽与固相接触; 并测量与固定化多肽结合的标记多肽的报道分子。本发明允许更快和更快速的夹心ELISA来测量高灵敏度的抗原浓度。

3. 发明申请

US20060252028A1 Method of assaying interaction between proteins 审中-公开
标题翻译：测定蛋白质之间相互作用的方法
公开(公告)号：US20060252028A1
公开(公告)日：2006-11-09
申请号：US10524564
申请日：2003-08-15
申请人： Hiroshi Ueda , Teruyuki Nagamune
发明人： Hiroshi Ueda , Teruyuki Nagamune
IPC分类号： C12Q1/70 , C07H21/04 , C12P21/06 , C12N1/21 , C07K16/18 , C12N15/74
CPC分类号： C40B40/02 , C12N15/1037 , C12N15/1055 , C12N15/70
摘要： A novel method for determining interaction between VH fragment and VL fragment of the variable region of an antibody is provided by the present invention. The method according to this invention can be widely used for detection of protein interactions. If a phagemid vector containing an amber codon is used for transformation of an amber suppressor strain of E. coli according to the method of the invention to produce phages, both of the VH fragment and the VL fragment will be displayed on the phage particles. In contrast, if the same vector is used to transformation of an amber non-suppressing strain of E. coli to produce phages, for the presence of the amber codon, only the VH fragment will be displayed on the phage particles, while the VL fragment will be secreted into the culture medium by the E. coli. Thus, display switch occurs. The interaction between the VH fragment and the VL fragment can be determined, by immobilizing the VL fragment secreted into the culture medium on a solid phase and quantifying binding between the VH fragment displayed by phages and the VL fragment.
摘要翻译：本发明提供了用于确定抗体可变区的VH片段和VL片段之间相互作用的新方法。根据本发明的方法可广泛用于蛋白质相互作用的检测。如果含有琥珀密码子的噬菌体载体用于根据本发明的方法转化大肠杆菌的琥珀抑制菌株以产生噬菌体，那么VH片段和VL片段都将显示在噬菌体颗粒上。相比之下，如果使用相同的载体转化大肠杆菌的琥珀非抑制菌株以产生噬菌体，则对于琥珀密码子的存在，只有VH片段将显示在噬菌体颗粒上，而VL片段将被大肠杆菌分泌到培养基中。因此，显示切换发生。可以通过将分泌到培养基中的VL片段固定在固相上并定量噬菌体展示的VH片段与VL片段之间的结合来确定VH片段和VL片段之间的相互作用。

4. 发明授权

US5981199A Method for measuring antigen concentration 失效
标题翻译：抗原浓度测定方法
公开(公告)号：US5981199A
公开(公告)日：1999-11-09
申请号：US663922
申请日：1996-06-14
申请人： Hiroshi Ueda , Teruyuki Nagamune , Hajime Nishimura , Izumi Kumagai , Kouhei Tsumoto , Walter C. Mahoney , Greg Winter , Paula A. Schueler
发明人： Hiroshi Ueda , Teruyuki Nagamune , Hajime Nishimura , Izumi Kumagai , Kouhei Tsumoto , Walter C. Mahoney , Greg Winter , Paula A. Schueler
IPC分类号： C07K16/18 , G01N33/68 , G01N33/573 , G01N33/53 , G01N33/543
CPC分类号： G01N33/6845 , C07K16/18 , G01N33/68 , G01N33/6857
摘要： This invention provides a method for measuring an antigen concentration in a sample, which comprises: preparing VH-domain polypeptide and VL-domain polypeptide of an antibody specific to the antigen; labeling one of the polypeptides with a reporter molecule to form labeled polypeptides, and immobilizing the other polypeptides onto solid-phase to form immobilized polypeptides; contacting the antigen-containing sample and the labeled polypeptides with the solid-phase; and measuring the reporter molecule of the labeled polypeptides bound to the immobilized polypeptides. The present invention permits simpler and quicker sandwich ELISA for measurements of an antigen concentration in high sensitivity.
摘要翻译：本发明提供了一种测定样品中抗原浓度的方法，其包括：制备抗原特异性抗体的VH结构域多肽和VL结构域多肽; 用报告分子标记多肽之一以形成标记的多肽，并将其它多肽固定在固相上以形成固定的多肽; 使含抗原的样品和标记的多肽与固相接触; 并测量与固定化多肽结合的标记多肽的报道分子。本发明允许更快和更快速的夹心ELISA来测量高灵敏度的抗原浓度。

5. 发明授权

US09023835B2 Agent for ameliorating blood-brain barrier disorders 有权
标题翻译：改善血脑屏障障碍的药剂
公开(公告)号：US09023835B2
公开(公告)日：2015-05-05
申请号：US13390058
申请日：2010-08-09
申请人： Hiroshi Ueda
发明人： Hiroshi Ueda
IPC分类号： A61K38/22 , A61K45/06
CPC分类号： A61K38/2292 , A61K45/06 , A61K2300/00
摘要： The invention provides a method of ameliorating a blood-brain barrier disorder, as well as a method of treating a disease accompanied by a blood-brain barrier disorder and a method of treating a cerebral ischemic disease, by administering a prothymosin α, or a protein or polypeptide possessing the same function as the prothymosin α. The invention also provides polypeptides useful in the context of the aforesaid methods.
摘要翻译：本发明提供一种改善血脑屏障障碍的方法，以及治疗伴有血脑屏障障碍的疾病的方法和治疗脑缺血疾病的方法，通过施用孕激素α或蛋白质或具有与胸腺素α相同功能的多肽。本发明还提供了在上述方法的上下文中有用的多肽。

6. 发明申请

US20130203738A1 USE OF COMPOUND BINDING TO MSIN3B THAT SPECIFICALLY BINDS TO NEURON RESTRICTIVE SILENCER FACTOR (NRSF) 有权
标题翻译：使用化合物结合到MSIN3B，特异性结合到神经元限制性沉默因子（NRSF）
公开(公告)号：US20130203738A1
公开(公告)日：2013-08-08
申请号：US13578230
申请日：2011-02-09
申请人： Yoshifumi Nishimura , Aritaka Nagadoi , Yuuka Hirao , Yoshio Goshima , Naoya Yamashita , Naoki Miyata , Takayoshi Suzuki , Ryuta Hiraishi , Hiroshi Ueda
发明人： Yoshifumi Nishimura , Aritaka Nagadoi , Yuuka Hirao , Yoshio Goshima , Naoya Yamashita , Naoki Miyata , Takayoshi Suzuki , Ryuta Hiraishi , Hiroshi Ueda
IPC分类号： C07D211/16 , C07D211/10 , C07D265/30 , C07D207/06 , C07D223/04 , C07D225/02 , C07D211/14 , C07C233/65
CPC分类号： C07D211/16 , A61K31/445 , C07C233/65 , C07C233/66 , C07C2601/14 , C07D207/06 , C07D211/10 , C07D211/14 , C07D211/96 , C07D223/04 , C07D225/02 , C07D265/30 , C07D295/192
摘要： The present invention identifies a compound which binds to the PAH1 domain of mSin3B that specifically binds to neural restrictive silencer factor NRSF, and uses the compound as a prophylactic and/or a therapeutic for diseases associated with abnormal expression of neural restrictive silencer factor NRSF/REST or abnormal expression of genes targeted by NRSF/REST, such as Huntington's disease, medulloblastoma and neuropathic pain.The present invention provides a pharmaceutical composition comprising a substance capable of binding to the PAH1 domain of mSin3B, e.g., a compound represented by the following formula (I), a pharmacologically acceptable salt thereof, or a pharmacologically acceptable ester thereof: wherein n represents 0 or 1; R1, R2, R3, R4 and R5 each independently represent a hydrogen atom, a hydrocarbon group or a functional group; Y represents a single bond, a carbonyl group, —CONH—, —NHCO— or a sulfonyl group; and Z represents a nitrogen-containing heterocyclic group which may have a substituent, an amino group which may have a hydrocarbon group or an aromatic hydrocarbon group, or a nitrogen and oxygen-containing heterocyclic group which may have a substituent.
摘要翻译：本发明鉴定了与特异性结合神经限制性沉默因子NRSF的mSin3B的PAH1结构域结合的化合物，并且将该化合物用作与神经限制性消音因子NRSF / REST的异常表达相关的疾病的预防和/或治疗或NRSF / REST靶向的基因异常表达，如亨廷顿氏病，成神经管细胞瘤和神经性疼痛。本发明提供一种药物组合物，其包含能够结合mSin3B的PAH1结构域的物质，例如下式（I）表示的化合物，其药理学上可接受的盐或其药理学上可接受的酯：其中n表示0 或1; R1，R2，R3，R4和R5各自独立地表示氢原子，烃基或官能团; Y表示单键，羰基，-CONH-，-NHCO-或磺酰基; Z表示可以具有取代基的含氮杂环基，可以具有烃基或芳香族烃基的氨基或可以具有取代基的含氮和含氧杂环基。

7. 发明授权

US08411196B2 Digital camera with movable mirror for AF in live view mode and optical viewfinder mode 有权
标题翻译：带实时取景模式和光学取景器模式的AF的可移动镜头的数码相机
公开(公告)号：US08411196B2
公开(公告)日：2013-04-02
申请号：US12095818
申请日：2006-11-30
申请人： Hiroshi Ueda , Kenichi Honjo , Naoto Yumiki , Toshio Makabe , Kenji Maeda , Kaoru Mokunaka , Kazuhiko Ishimaru
发明人： Hiroshi Ueda , Kenichi Honjo , Naoto Yumiki , Toshio Makabe , Kenji Maeda , Kaoru Mokunaka , Kazuhiko Ishimaru
IPC分类号： H04N5/232
CPC分类号： H04N5/23203 , G03B19/00 , G03B19/12 , H04N5/2254 , H04N5/23212 , H04N5/23245 , H04N5/23293 , H04N5/238
摘要： A digital camera of the present invention includes a microcomputer 110 having a live view mode controlling so that image data generated by a CMOS sensor 130 or image data obtained by subjecting the image data generated by the CMOS sensor 130 to predetermined processing is displayed on a liquid crystal monitor 150 as a moving image in real time, wherein when a release button 141 receives an instruction regarding start of an autofocus operation in a live view mode, the microcomputer 110 controls a movable mirror to enter an optical path to measure by an AF sensor 132, and thereafter, allow the movable mirror to retract from the optical path to return the digital camera to the live view mode. Due to this configuration, in a digital camera that includes a movable mirror and is capable of displaying a subject image in a live view through an electronic viewfinder, the operability thereof can be enhanced.
摘要翻译：本发明的数码相机包括具有实时取景模式控制的微型计算机110，使得由CMOS传感器130生成的图像数据或通过对由CMOS传感器130生成的图像数据进行预定处理而获得的图像数据显示在液体晶体监视器150作为运动图像实时地实现，其中当释放按钮141接收到关于在实时取景模式中的自动对焦操作的开始的指令时，微型计算机110控制可移动反射镜进入光学路径以由AF传感器 132，然后允许可移动镜从光路缩回以将数字照相机返回到实时取景模式。由于这种结构，在包括可移动镜并且能够通过电子取景器在实时取景中显示被摄体图像的数码相机中，可以提高其可操作性。

8. 发明申请

US20120270338A1 FLUOROIMMUNOASSAY METHOD 审中-公开
标题翻译：荧光分光法
公开(公告)号：US20120270338A1
公开(公告)日：2012-10-25
申请号：US13510105
申请日：2010-11-19
申请人： Hiroshi Ueda , Ryoji Abe , Masaki Ihara , Hiroaki Takagi
发明人： Hiroshi Ueda , Ryoji Abe , Masaki Ihara , Hiroaki Takagi
IPC分类号： G01N21/76 , C07K17/02
CPC分类号： G01N33/582 , A61K49/0058 , C07K16/18 , C07K16/44 , C07K2317/56 , C07K2317/622 , C07K2317/92 , G01N21/6428 , G01N33/6857 , G01N2021/6439
摘要： An object is to provide an immunoassay method requiring neither a solid-phase immobilization step nor a washing step, enabling quick and simple quantitative measurement of a target substance in a liquid phase and capable of visualizing an antigen. Such an object is attained by measuring the concentration of a target antigen present in a test substance by sequentially performing a step (a) of bringing an antibody light-chain variable region polypeptide and an antibody heavy-chain variable region polypeptide labeled with a fluorescent dye into contact with an antigen in a test substance in a liquid phase; or bringing an antibody heavy-chain variable region polypeptide and an antibody light-chain variable region polypeptide labeled with a fluorescent dye into contact with an antigen in a test substance in a liquid phase; a step (b) of measuring the fluorescence intensity of the fluorescent dye; and a step (c) of computationally obtaining the level of the antigen contained in the test substance with reference to a positive correlation between the concentration of the antigen in a liquid phase and the fluorescence intensity of the fluorescent dye.
摘要翻译：本发明的目的是提供一种不需要固相固定步骤和洗涤步骤的免疫测定方法，能够快速和简单地定量测定液相中的目标物质并能够使抗原呈现。通过依次进行使荧光染料标记的抗体轻链可变区多肽和抗体重链可变区多肽的步骤（a），测定待测物质中存在的靶抗原的浓度，与液相中测试物质中的抗原接触; 或使用荧光染料标记的抗体重链可变区多肽和抗体轻链可变区多肽与液相中的测试物质中的抗原接触; 测量荧光染料的荧光强度的步骤（b）; 和（c）参考在液相中的抗原浓度与荧光染料的荧光强度之间的正相关性来计算获得测试物质中含有的抗原水平的步骤（c）。

9. 发明授权

US08223263B2 Digital camera 有权
公开(公告)号：US08223263B2
公开(公告)日：2012-07-17
申请号：US12831001
申请日：2010-07-06
申请人： Hiroshi Ueda , Kenichi Honjo , Naoto Yumiki , Toshio Makabe , Kenji Maeda , Kaoru Mokunaka , Kazuhiko Ishimaru
发明人： Hiroshi Ueda , Kenichi Honjo , Naoto Yumiki , Toshio Makabe , Kenji Maeda , Kaoru Mokunaka , Kazuhiko Ishimaru
IPC分类号： H04N5/225
CPC分类号： H04N5/23203 , G03B19/00 , G03B19/12 , H04N5/2254 , H04N5/23212 , H04N5/23245 , H04N5/23293 , H04N5/238
摘要： A digital camera of the present invention includes a microcomputer 110 having a live view mode controlling so that image data generated by a CMOS sensor 133 or image data obtained by subjecting the image data generated by the CMOS sensor 133 to predetermined processing is displayed on a liquid crystal monitor 150 as a moving image in real time, wherein the microcomputer 110 controls so that, when the live view mode is set, the digital camera comes out of the live view mode, and setting information on the digital camera is displayed on the liquid crystal monitor 150, in accordance with the manipulation of the manipulation portion 140. Due to this configuration, in a digital camera that includes a movable mirror and is capable of displaying a subject image in a live view through an electronic viewfinder, the operability thereof can be enhanced.

10. 发明授权

US08077560B2 Additionally recordable information recording medium, information recording method, information reproducing method, information recording apparatus and information reproducing apparatus 有权
标题翻译：信息记录介质，信息记录方法，信息再现方法，信息记录装置和信息再现装置
公开(公告)号：US08077560B2
公开(公告)日：2011-12-13
申请号：US12608173
申请日：2009-10-29
申请人： Hiroshi Ueda , Motoshi Ito
发明人： Hiroshi Ueda , Motoshi Ito
IPC分类号： G11B7/00
CPC分类号： G11B27/105 , G11B7/00736 , G11B20/1217 , G11B27/329 , G11B2220/218 , G11B2220/2562
摘要： An additionally recordable information recording medium has a complicated management structure for managing an unrecorded area. Therefore, there are problems that it takes a long time for a disk to startup and it is difficult to build a recording/reproducing system. The additionally recordable information recording medium according to the present invention includes a simple data structure for managing a location information for a recorded area located at the rearmost location in a user data area. An additionally recordable apparatus according to the present invention also includes means for updating the location information of the recorded area so that the recorded area located at the rearmost location in the user data area is always managed.
摘要翻译：另外可记录的信息记录介质具有用于管理未记录区域的复杂管理结构。因此，存在磁盘启动需要很长时间并且难以构建记录/再现系统的问题。根据本发明的另外可记录的信息记录介质包括用于管理位于用户数据区域中最后位置的记录区域的位置信息的简单数据结构。根据本发明的另外可记录的装置还包括用于更新记录区域的位置信息的装置，使得位于用户数据区域中的最后位置的记录区域总是被管理。

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