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1. 发明专利

JP2010115136A Method for creating membrane protein with labelled n-terminal end, and cell having membrane protein with labelled n-terminal end in its outer layer 审中-公开
标题翻译：用标记的N末端端形成膜蛋白的方法，以及具有标记的N末端末端的膜蛋白的细胞外层
公开(公告)号：JP2010115136A
公开(公告)日：2010-05-27
申请号：JP2008290026
申请日：2008-11-12
申请人： Teruyuki Nagamune , 輝行長棟
发明人： NAGAMUNE TERUYUKI , YAMAMOTO AKIYASU , TANAKA TSUTOMU , TSUKIJI MASAYA
IPC分类号： C12N15/09 , A61K35/12 , A61P43/00 , C12N5/10 , C12P21/02 , C12Q1/02 , G01N21/78 , G01N33/48
摘要： PROBLEM TO BE SOLVED: To provide a method for specifically labelling only a target protein by modifying a specific probe to the target protein, a method for labelling a membrane protein in which its N-terminal end exists extracellularly, a cell having a membrane protein specifically modified by the method, and a method for screening using the cell. SOLUTION: There is disclosed a method for creating a membrane protein with a labelled N-terminal end, wherein the method is featured by using a labelled molecular probe in which a membrane protein having LPX 1 X 2 (G) n sequence (X 1 is an optional amino acid, and X 2 represents threonine or alanine. n is any one of an integer from 2 to 8.) in a region of N-terminal side and having an N-terminal end extracellularly existing, a peptide transferring enzyme Sortase A, and LPX 1 X 2 (G) m sequence (X 1 is an optional amino acid, and X 2 represents threonine or alanine. m is 1 or 2.) are added. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：待解决的问题：为了提供通过将特异性探针修饰为靶蛋白来仅特异性标记靶蛋白的方法，用于标记其N-末端存在于细胞外的膜蛋白的方法，具有通过该方法特异性修饰的膜蛋白，以及使用该细胞进行筛选的方法。解决方案：公开了一种用于产生具有标记的N-末端的膜蛋白的方法，其中所述方法的特征在于使用标记的分子探针，其中具有LPX 1的膜蛋白（SP）（G） n 序列（X 1 是任选的氨基酸，X 2 代表苏氨酸或丙氨酸 n为2〜8的整数），N末端侧的区域为胞外存在的N-末端，肽转移酶分选酶A和LPX 1 （SP）（G） m 序列（X 1 是任选的氨基酸，X 2 代表苏氨酸或丙氨酸 m为1或2.）。版权所有（C）2010，JPO＆INPIT

2. 发明专利

JP2004161731A Fixing agent for biorelating substance 审中-公开
公开(公告)号：JP2004161731A
公开(公告)日：2004-06-10
申请号：JP2003091099
申请日：2003-03-28
申请人： Teruyuki Nagamune , Nof Corp , 日本油脂株式会社 , 輝行長棟
发明人： NAGAMUNE TERUYUKI , KATO KOICHI , SHINKAI MASASHIGE , KITANO SHIGERU , YASUKOCHI TORU
IPC分类号： A61C13/08 , A61F2/06 , A61F2/10 , A61F2/28 , A61K6/02 , A61K8/96 , A61K8/98 , A61K9/70 , A61K47/48 , A61L27/00 , C12N5/07 , C12N5/077 , A61K7/00 , C12N5/06
摘要： PROBLEM TO BE SOLVED: To provide a fixing agent for easily fixing a biorelating substance to living cell or living tissue without damaging the living cell and living tissue and provide a fixing method with the fixing agent. SOLUTION: The fixing agent for a biorelating substance contains 1-100 wt.% amphiphilic compound containing (1) one or more 15-23C unsaturated aliphatic hydrocarbon groups on one terminal, (2) one or more parts containing a polyoxyalkylene chain in the molecule and (3) one or more reactive functional groups on the terminal different from the terminal of the item (1). COPYRIGHT: (C)2004,JPO

3. 发明专利

JP2004113062A Animal cell having chimera receptor and its utilization 审中-公开
公开(公告)号：JP2004113062A
公开(公告)日：2004-04-15
申请号：JP2002279019
申请日：2002-09-25
申请人： Teruyuki Nagamune , 長棟輝行
发明人： NAGAMUNE TERUYUKI , KAWAHARA MASAHIRO , UEDA HIROSHI , SHINKAI MASASHIGE
IPC分类号： G01N33/48 , A61K35/76 , A61K48/00 , A61P35/00 , C07K16/44 , C12N5/10 , C12N15/09 , C12P1/00 , C12Q1/02 , C12Q1/68 , G01N33/15 , G01N33/50 , G01N33/53 , G01N33/566 , G01N33/574
CPC分类号： G01N33/5091 , C07K16/44 , C07K2319/00 , C12N2510/00 , G01N33/5094
摘要： PROBLEM TO BE SOLVED: To provide an animal cell which selectively causes an immune response in the presence of an antigen specifically recognizing a single strand antibody, to provide a vector DNA which holds a recombinant gene for forming the animal cell, to provide a method for producing a useful substance with the animal cell and a vector DNA, to provide a method for measuring the concentration of the antigen, to provide a method for screening a cell response-activating substance, to provide a method for measuring an interprotein interaction, to provide a method for controlling the cell response, to provide a method for screening a chimera receptor, to provide a method for selecting a foreign gene transgenic cell, and to provide a virus vector DNA for treating cancers, and the like. SOLUTION: The transgenic cell which can be proliferated in a state not dependent on a cell proliferation factor by the stimulation of fluorescein dimer is created by transducing a vector DNA holding a mouse erythropoietin receptor gene, and a recombinant gene of a mouse gp 130 gene and a single strand antibody 311J3 gene against fluorescein into a target cell, thereby expressing the chimera receptor on the membrane surface of the cell. COPYRIGHT: (C)2004,JPO

4. 发明授权

US06482596B1 Method for measuring antigen concentration 有权
标题翻译：抗原浓度测定方法
公开(公告)号：US06482596B1
公开(公告)日：2002-11-19
申请号：US09313350
申请日：1999-05-18
申请人： Hiroshi Ueda , Teruyuki Nagamune , Hajime Nishimura , Izumi Kumagai , Kouhei Tsumoto , Walter C. Mahoney , Greg Winter , Paula A. Schueler
发明人： Hiroshi Ueda , Teruyuki Nagamune , Hajime Nishimura , Izumi Kumagai , Kouhei Tsumoto , Walter C. Mahoney , Greg Winter , Paula A. Schueler
IPC分类号： G01N3353
CPC分类号： G01N33/6845 , C07K16/18 , G01N33/68 , G01N33/6857
摘要： This invention provides a method for measuring an antigen concentration in a sample, which comprises: preparing VH-domain polypeptide and VL-domain polypeptide of an antibody specific to the antigen; labeling one of the polypeptides with a reporter molecule to form labeled polypeptides, and immobilizing the other polypeptide onto solid-phase to form immobilized polypeptides; contacting the antigen-containing sample and the labeled polypeptides with the solid-phase; and measuring the reporter molecule of the labeled polypeptides bound to the immobilized polypeptides. The present invention permits simpler and quicker sandwich ELISA for measurements of an antigen concentration in high sensitivity.
摘要翻译：本发明提供了一种测定样品中抗原浓度的方法，包括：制备抗原特异性抗体的VH结构域多肽和VL结构域多肽; 用报告分子标记多肽之一以形成标记的多肽，并将另一多肽固定在固相上以形成固定的多肽; 使含抗原的样品和标记的多肽与固相接触; 并测量与固定化多肽结合的标记多肽的报道分子。本发明允许更快和更快速的夹心ELISA来测量高灵敏度的抗原浓度。

5. 发明授权

US5334497A Method of feeding a substrate into tubular bioreactor 失效
标题翻译：将基质喂入管状生物反应器的方法
公开(公告)号：US5334497A
公开(公告)日：1994-08-02
申请号：US786819
申请日：1991-11-04
申请人： Hideki Inaba , Isao Endo , Teruyuki Nagamune , Susumu Tachikawa
发明人： Hideki Inaba , Isao Endo , Teruyuki Nagamune , Susumu Tachikawa
IPC分类号： C12M1/12 , C12M1/36 , C12Q3/00
CPC分类号： C12M23/06 , C12M29/16 , C12M29/18 , C12M41/00 , C12M41/40 , Y10S435/813
摘要： A method of feeding substrates into a tubular bioreactor incorporating a reacting vessel and a separating membrane in which the reaction and separation or collection can be continuously and surely effected in the same vessel with high efficiency.
摘要翻译：将基材输送到包含反应容器和分离膜的管状生物反应器中的方法，其中反应和分离或收集可以在高效率的同一容器中连续且可靠地进行。

6. 发明授权

US07198945B2 Cell having modified cell membrane 有权
标题翻译：细胞具有改变的细胞膜
公开(公告)号：US07198945B2
公开(公告)日：2007-04-03
申请号：US10211587
申请日：2002-08-05
申请人： Teruyuki Nagamune , Chika Itoh , Tohru Yasukohchi , Syunsuke Ohhashi , Kazuhiro Kubo
发明人： Teruyuki Nagamune , Chika Itoh , Tohru Yasukohchi , Syunsuke Ohhashi , Kazuhiro Kubo
IPC分类号： C12N5/00
CPC分类号： C12N5/0006
摘要： A cell in which a reaction product of a substance to be modified and an amphipathic compound is non-covalently bound to a cell membrane, wherein said compound has the following features: (1) having one or more aliphatic hydrocarbon groups at one end; (2) having one or more portions containing a hydrophilic group in a molecule; and (3) having one or more reactive functional groups which are capable of covalently binding with the substance to be modified at an end different from the end in the above (1).
摘要翻译：其中待修饰物质和两亲性化合物的反应产物与细胞膜非共价结合的细胞，其中所述化合物具有以下特征

7. 发明申请

US20050208644A1 Method of immobilizing cells on solid-phase surface 审中-公开
标题翻译：将细胞固定在固相表面上的方法
公开(公告)号：US20050208644A1
公开(公告)日：2005-09-22
申请号：US11085184
申请日：2005-03-22
申请人： Hiroshi Takiguchi , Hitoshi Fukushima , Takashi Masuda , Teruyuki Nagamune , Masashige Shinkai , Kengo Yamawaki
发明人： Hiroshi Takiguchi , Hitoshi Fukushima , Takashi Masuda , Teruyuki Nagamune , Masashige Shinkai , Kengo Yamawaki
IPC分类号： C12M1/40 , C10G32/00 , C12M3/00 , C12N11/00 , C12N11/02
CPC分类号： B82Y30/00 , B01J2219/00659 , B01J2219/00743 , C12N11/00
摘要： Aspects of the invention can provide a method of immobilizing a chemical compound having the affinity for the cell membrane on the solid-phase surface in a desired pattern. The method of immobilizing a cell in a desired pattern on a solid-phase surface by use of a first chemical compound having an affinity for the cell and can include a step of immobilizing a second chemical compound, which is more easily immobilized on the solid-phase surface than the first chemical compound dose and has a molecular binding site that can bind to the first chemical compound, on the solid-phase surface according to the pattern.
摘要翻译：本发明的方面可以提供一种将所述细胞膜具有亲和性的化合物以期望的图案固定在固相表面上的方法。通过使用对细胞具有亲和力的第一化合物，将固定相的表面上的细胞以期望的图案固定的方法，其可以包括将更容易固定在固相上的第二化合物固定化的步骤，相表面比第一化合物剂量，并且具有可以根据图案在固相表面上结合第一化合物的分子结合位点。

8. 发明授权

US5185255A Cell culture method 失效
标题翻译：细胞培养法
公开(公告)号：US5185255A
公开(公告)日：1993-02-09
申请号：US504694
申请日：1990-04-04
申请人： Isao Endo , Teruyuki Nagamune , Minoru Nishimura , Tetsuo Kobayashi
发明人： Isao Endo , Teruyuki Nagamune , Minoru Nishimura , Tetsuo Kobayashi
IPC分类号： C12M1/16 , C12M3/00
CPC分类号： C12M33/16 , C12M25/16 , C12M29/26 , C12M41/40 , C12M47/02 , Y10S435/822 , Y10S435/911 , Y10S435/918
摘要： A cell culture method proliferates microorganisms, particularly fungi such as molds, actinomycetes, animal cells, plant cells and so on to produce useful substances such as antibiotics, enzymes, proteins, polysaccharides, physiological active substances, and animal and plant hormones. Porous material including medium and cells to be proliferated is moved, whereby air is supplied to the surface of the porous material.
摘要翻译：细胞培养方法增殖微生物，特别是真菌如霉菌，放线菌，动物细胞，植物细胞等，以产生有用的物质，如抗生素，酶，蛋白质，多糖，生理活性物质和动植物激素。包括培养基在内的多孔材料和要增殖的细胞移动，由此向多孔材料的表面供应空气。

9. 发明授权

US4725148A Turbidimeter employing a semiconductor laser diode and a photodiode 失效
标题翻译：浊度计采用半导体激光二极管和光电二极管
公开(公告)号：US4725148A
公开(公告)日：1988-02-16
申请号：US739319
申请日：1985-05-30
申请人： Isao Endo , Teruyuki Nagamune , Ichiro Inoue , Kozo Inoue , Tadashi Nohira , Ikuzo Kagami , Tatsuya Iwakura
发明人： Isao Endo , Teruyuki Nagamune , Ichiro Inoue , Kozo Inoue , Tadashi Nohira , Ikuzo Kagami , Tatsuya Iwakura
IPC分类号： G01N21/59 , G01N21/53 , G01N21/85 , G01N21/84
CPC分类号： G01N21/8507 , G01N21/534 , G01N2201/0621 , G01N2201/0631 , G01N2201/064
摘要： In a turbidimeter for measuring a turbidity of a test solution to be measured i.e. culture solution in a fermentation apparatus, a semiconductor laser diode and a semiconductor photodiode are integrally arranged in a detection portion of the turbidimeter in such a manner that a laser beam emitted from the semiconductor laser diode is made incident upon the semiconductor photodiode through the test solution. Moreover, a protection circuit for the semiconductor laser diode and the semiconductor photodiode is also arranged in the turbidimeter to cut off a current flowed therethrough when an environmental temperature becomes above a predetermined temperature. Therefore, the turbidimeter can be made small in size and light in weight, and the turbidity can be measured accurately over wide range.
摘要翻译：在浊度计中，用于测量待测试的测试溶液的浊度，即发酵设备中的培养溶液，半导体激光二极管和半导体光电二极管一体地布置在浊度计的检测部分中，使得从半导体激光二极管通过测试溶液入射到半导体光电二极管上。此外，当环境温度高于预定温度时，用于半导体激光二极管和半导体光电二极管的保护电路也布置在浊度计中以截断流过其中的电流。因此，可以使浊度计尺寸小，重量轻，并且可以在宽范围内精确地测量浊度。

10. 发明授权

US4501161A Autosampler 失效
标题翻译：自动进样器
公开(公告)号：US4501161A
公开(公告)日：1985-02-26
申请号：US459598
申请日：1983-01-20
申请人： Isao Endo , Teruyuki Nagamune , Ichiro Inoue
发明人： Isao Endo , Teruyuki Nagamune , Ichiro Inoue
IPC分类号： G01N1/14
CPC分类号： G01N1/14
摘要： This invention relates to an apparatus for automatically sampling a clarified test solution from a suspension to be tested, which comprises two filtering tubes being immersed in the suspension, a circulator connected to the one tube, and a sampling cell connected to the circulator and the other tube. The suspension is sucked by the circulator and filtered through the one tube. The filtered and clarified test solution is sampled in the sampling cell and the residual test solution is returned to the suspension through the other tube. A flow direction of the test solution is reversed at intervals so that the filtering tubes are not blocked by suspending solids.
摘要翻译：本发明涉及一种用于从待测试的悬浮液中自动采样澄清的测试溶液的装置，其包括浸入悬浮液中的两个过滤管，连接到一个管的循环器和连接到循环器的另一个采样单元管。悬浮液被循环器吸入并通过一个管过滤。将过滤和澄清的测试溶液在采样池中取样，并将残留的测试溶液通过另一个管返回到悬浮液。测试溶液的流动方向间隔地反向，使得过滤管不被悬浮固体堵塞。

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