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1. 发明专利

JP2005143379A Method and apparatus for recovering microorganism 审中-公开
标题翻译：用于恢复微生物的方法和装置
公开(公告)号：JP2005143379A
公开(公告)日：2005-06-09
申请号：JP2003384815
申请日：2003-11-14
申请人： Tadashi Matsunaga , Haruko Takeyama , Toshiba Corp , 松永是 , 株式会社東芝 , 竹山春子
发明人： MATSUNAGA TADASHI , TAKEYAMA HARUKO , YOSHINO MASATO , HARAGUCHI SATOSHI , TOYODA HIROKAZU , KANEKO MASAO
IPC分类号： C12M1/12 , B01D63/02 , B01D65/02 , C12N1/02
摘要： PROBLEM TO BE SOLVED: To provide a method and an apparatus for recovering microorganisms, by which a large amount of microorganisms are treated in high efficiency.
SOLUTION: In recovering object microorganisms in water, the object microorganisms in water are separated and concentrated by using a hollow fiber membrane of cross flow filtration type. The object microorganisms separated and concentrated by the hollow fiber membrane are subjected to reversal washing with a solution in an amount smaller than water before the separation and concentration, released from the hollow fiber membrane, further separated, concentrated and immobilized by using a membrane filter from the solution having the object microorganisms concentrated by the reversal washing after the reversal washing.
COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：待解决的问题：提供一种用于回收微生物的方法和装置，通过该方法和装置高效率地处理大量的微生物。解决方案：在水中回收微生物时，通过使用交叉流过滤型中空纤维膜将水中的微生物分离和浓缩。通过中空纤维膜分离和浓缩的目的微生物用分离和浓缩的溶液进行反转洗涤，分离和浓缩，从中空纤维膜释放，进一步分离，浓缩和固定，使用膜过滤器在反转洗涤后，通过反转洗涤将具有物体微生物的溶液浓缩。版权所有（C）2005，JPO＆NCIPI

2. 发明专利

JP2004101361A Method and method for detecting cryptosporidium 有权
标题翻译：检测焦磷酸钠的方法和方法
公开(公告)号：JP2004101361A
公开(公告)日：2004-04-02
申请号：JP2002263318
申请日：2002-09-09
申请人： Tadashi Matsunaga , Haruko Takeyama , Toshiba Corp , 松永是 , 株式会社東芝 , 竹山春子
发明人： MATSUNAGA TADASHI , TAKEYAMA HARUKO , HARAGUCHI SATOSHI , YOSHINO MASATO , IYASU KYOTARO , KANEKO MASAO , FUJISAWA MINORU
IPC分类号： G01N33/569 , C12M1/34 , C12Q1/06 , C12Q1/68 , G01N33/18 , G01N33/48 , G01N33/53 , G01N33/566
CPC分类号： Y02A50/451
摘要： PROBLEM TO BE SOLVED: To provide a highly accurate Cryptosporidium detecting method capable of highly efficiently processing large quantities and to provide an apparatus for implementing the method.
SOLUTION: In the detection method, microorganisms in sample water are isolated through the use of a hollow fiber membrane (11), condensed, further isolated through the use of a membrane filter (14), and condensed. An oocyst outer shell of Cryptosporidium among acquired microorganisms is damaged. By performing both fluorescent emission in-situ hybridization through the use of a probe complementary to nucleic acids contained in the Cryptosporidium and immuno-staining through the use of an antibody to matter related to the Cryptosporidium to perform double-staining, a differential interference image and a fluorescent emission image of the double-stained microorganisms are acquired and analyzed to recognize the Cryptosporidium.
COPYRIGHT: (C)2004,JPO
摘要翻译：要解决的问题：提供能够高效处理大量的高精度隐孢子虫检测方法，并提供实现该方法的装置。解决方案：在检测方法中，通过使用中空纤维膜（11）分离样品水中的微生物，冷凝，通过使用膜过滤器（14）进一步分离，并浓缩。获得性微生物中隐孢子虫的卵囊外壳受损。通过使用与隐孢子虫中包含的核酸互补的探针进行荧光发射原位杂交，通过使用与隐孢子虫相关的物质的抗体进行免疫染色来进行双染色，差分干涉图像和获取并分析双重染色的微生物的荧光发射图像以识别隐孢子虫。版权所有（C）2004，JPO

3. 发明专利

JP2006262825A Apparatus for separating microorganism 有权
标题翻译：用于分离微生物的装置
公开(公告)号：JP2006262825A
公开(公告)日：2006-10-05
申请号：JP2005088306
申请日：2005-03-25
申请人： Tadashi Matsunaga , Haruko Takeyama , Toshiba Corp , 松永是 , 株式会社東芝 , 竹山春子
发明人： MATSUNAGA TADASHI , TAKEYAMA HARUKO , HARAGUCHI SATOSHI , YOSHINO MASATO , ISHIMORI YOSHIO , KANEKO MASAO , TOYODA HIROKAZU
IPC分类号： C12M1/00 , C12M1/42 , G01N1/04
CPC分类号： C12M47/02 , C12M33/14
摘要： PROBLEM TO BE SOLVED: To provide an apparatus for separating a microorganism designed to selectively capture, separate and recover the microorganism which is the object of detection in a micro-region. SOLUTION: The specific microorganism contained in sample water is captured, separated and recovered. In the process, the following apparatus is provided. The apparatus for separating the microorganism is equipped with a micro-chip 1 having a flow channel 2 for the sample water formed on a substrate 1a and a capturing part 3 provided with a plurality of holes 4 for capturing the microorganism designed to capture the microorganism which is the object of separation by configurational characters thereof and arranged in the course of the flow channel and a sample water feeding means 10 for making the sample water containing the microorganism which is the object of separation flow into the micro-chip. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：提供一种用于分离设计用于在微区域中选择性捕获，分离和回收作为检测对象的微生物的微生物的装置。解决方案：捕获，分离和回收样品水中所含的特定微生物。在该过程中，提供以下装置。用于分离微生物的装置配备有具有用于在基板1a上形成的样品水的流路2的微芯片1和具有多个孔4的捕获部分3，捕获部分3用于捕获设计成捕获微生物的微生物，是通过其结构特征进行分离的目的并且设置在流路的过程中，以及用于使含有作为分离对象的微生物的样品水流入微芯片的样品供水装置10。版权所有（C）2007，JPO＆INPIT

4. 发明专利

JP2006262821A Method for preserving microbial membrane 有权
标题翻译：保存微生物膜的方法
公开(公告)号：JP2006262821A
公开(公告)日：2006-10-05
申请号：JP2005088157
申请日：2005-03-25
申请人： Tadashi Matsunaga , Haruko Takeyama , Toshiba Corp , 松永是 , 株式会社東芝 , 竹山春子
发明人： MATSUNAGA TADASHI , TAKEYAMA HARUKO , HARAGUCHI SATOSHI , KANEKO MASAO , MAOKA TADANORI , SHIROTA AKIHIKO , FUJISAWA MINORU , HIRAMOTO AKIRA
IPC分类号： C12Q1/02 , C12N11/12 , C12R1/01
摘要： PROBLEM TO BE SOLVED: To provide a method for preserving a microbial membrane with which the activity of a microorganism can be maintained over a long period without dipping the microorganism-immobilizing membrane in a liquid.
SOLUTION: The method for preserving the microbial membrane comprises detecting a specific substance using respiratory inhibition of an iron bacterium as an index. Furthermore, the method comprises a step of immobilizing the microorganism 1 in the immobilizing membrane 2, a step of housing the immobilizing membrane 2 in a holder for fixing electrodes, a step of preparing a humectant carrier 3 soaked with a humectant liquid for preventing drying of the immobilizing membrane 2 and a step of housing the holder 7 for fixing the electrodes and the humectant carrier 3 in a hermetically sealed container preventing the entry of oxygen together with an inert gas in such a state that the microorganism 1 immobilized in the immobilizing membrane 2 housed in the holder 7 for fixing the electrodes is kept out of contact with the humectant carrier 3.
COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：待解决的问题：提供一种用于保存微生物膜的方法，微生物膜可以长期保持其活性，而不将微生物固定膜浸没在液体中。解决方案：保存微生物膜的方法包括使用铁细菌的呼吸抑制作为指标检测特定物质。此外，该方法包括将微生物1固定在固定膜2中的步骤，将固定膜2容纳在用于固定电极的保持件中的步骤，制备用用于防止干燥的湿润剂液体浸湿的湿润剂载体3的步骤固定膜2和将电极和保湿剂载体3固定在气密密封容器中的保持器7的步骤，防止在固定在固定膜2中的微生物1的惰性气体中进入氧气容纳在用于固定电极的保持器7中，与保湿剂载体3保持不接触。版权所有（C）2007，JPO＆INPIT

5. 发明专利

JP2004101360A Method and apparatus for detecting microorganism 有权
公开(公告)号：JP2004101360A
公开(公告)日：2004-04-02
申请号：JP2002263299
申请日：2002-09-09
申请人： Tadashi Matsunaga , Haruko Takeyama , Toshiba Corp , 松永是 , 株式会社東芝 , 竹山春子
发明人： MATSUNAGA TADASHI , TAKEYAMA HARUKO , HARAGUCHI SATOSHI , YOSHINO MASATO , IYASU KYOTARO , KANEKO MASAO , FUJISAWA MINORU
IPC分类号： G01N21/64 , C12M1/34 , C12M1/40 , C12Q1/04 , C12Q1/68 , C12R1/90 , G01N21/77 , G01N21/78 , G01N33/53 , G01N33/543 , G01N33/566 , G01N33/569
摘要： PROBLEM TO BE SOLVED: To provide a method and an apparatus for detecting microorganisms capable of improving accuracy in consideration of indeterminate factors such as false positive without complicated processing.
SOLUTION: The detection method is provided with: a step for capturing solid matter of a microorganism to be detected in sample water containing the microorganism; a step for fixing the captured solid matter onto a fixing plate; a step for bringing a labelling antibody, for specifically dying the microorganism to be detected, into contact with the solid matter on the fixing plate; a step for acquiring a two-dimensional image by irradiating light for sorting out the dyed solid matter; a step for forming double strands by in-situ hybridization using a nucleic acid probe, which has a base sequence complementary to nucleic acids contained in the microorganism to be detected and is labeled differently from the labeling antibody; a step for acquiring a two-dimensional image by irradiating light for sorting out the solid matter which forms the double strands; and a step for recognizing an image which appears in the same coordinates among the two-dimensional images as the microorganism to be detected.
COPYRIGHT: (C)2004,JPO

6. 发明专利

JP2004097087A Method and system for recovering microorganism 有权
公开(公告)号：JP2004097087A
公开(公告)日：2004-04-02
申请号：JP2002263082
申请日：2002-09-09
申请人： Tadashi Matsunaga , Haruko Takeyama , Toshiba Corp , 松永是 , 株式会社東芝 , 竹山春子
发明人： MATSUNAGA TADASHI , TAKEYAMA HARUKO , YOSHINO MASATO , HARAGUCHI SATOSHI , IYASU KYOTARO , KANEKO MASAO , FUJISAWA MINORU
IPC分类号： C12M1/26 , B01D61/14 , B01D61/16 , B01D61/20 , B01D61/22 , B01D61/58 , B03C1/00 , C02F1/32 , C02F1/50 , C02F1/78 , C12N1/04 , C12Q1/24
CPC分类号： C12M47/02 , C12M29/16 , C12M33/14 , C12M47/04
摘要： PROBLEM TO BE SOLVED: To provide a method and a related system for recovering microorganisms in high efficiency on a large scale.
SOLUTION: The method for recovering chlorine-tolerant pathogenic microorganisms as the object microbes in a sample water comprises the steps of separating and concentrating the object microbes in the sample water using a hollow fiber membrane(11), back-washing the object microbes with a solution less than the sample water to release the microbes, further separating and concentrating the object microbes using a membrane filter(14) from the resultant back-washing solution where the object microbes are concentrated by the above back-washing operation, and releasing the resultant microbes with a solution less than the above back-washing solution.
COPYRIGHT: (C)2004,JPO

7. 发明专利

JP2004024981A Detaching device for disposable chip for dispensing device 审中-公开
公开(公告)号：JP2004024981A
公开(公告)日：2004-01-29
申请号：JP2002183061
申请日：2002-06-24
申请人： Juki Corp , Tadashi Matsunaga , ジューキ株式会社 , 松永是
发明人： UDAGAWA YUJI , NEMOTO ETSUO , MARUYAMA KOHEI , MATSUNAGA TADASHI
IPC分类号： G01N35/10 , B01J4/00 , B01L3/02
摘要： PROBLEM TO BE SOLVED: To provide a detaching device of disposable chip for dispensing device which can efficiently detach a disposable chip from a dispensing nozzle part using upward and downward movement of the nozzle part. SOLUTION: The disposable chip is inserted by descending it into a through-hole of a substrate having the through-hole of larger diameter than the outside diameter of the disposable chip in the course of descending of the nozzle part fitting and holding the disposable chip, while a chip insertion control mechanism preventing the disposable chip from ascending from the through-hole in the course of ascending of the nozzle part. COPYRIGHT: (C)2004,JPO

8. 发明专利

JP2004028683A Automatic dispensing apparatus and liquid dispense method 有权
公开(公告)号：JP2004028683A
公开(公告)日：2004-01-29
申请号：JP2002183064
申请日：2002-06-24
申请人： Juki Corp , Tadashi Matsunaga , ジューキ株式会社 , 松永是
发明人： NEMOTO ETSUO , MARUYAMA KOHEI , UDAGAWA YUJI , MATSUNAGA TADASHI
IPC分类号： G01N1/00 , G01N35/10
摘要： PROBLEM TO BE SOLVED: To smoothly deliver a liquid sample or specimen into a well without allowing inclusion of bubbles. SOLUTION: An automatic dispensing apparatus comprises a dispensing head having a nozzle for sucking/delivering a liquid in a sample container having a plurality of the wells, a shaft driving means for transferring the dispensing head in X, Y and Z axis directions, pistons for sucking/delivering the liquid through the nozzle, piston driving means for driving the pistons and a control data storage section storing control data including speed change profile of the driving means calculated on the basis of data on delivery amounts and well shapes. The apparatus is further provided with a drive control means for raising a distal end of a tip mounted on the nozzle when the liquid is delivered into the well according to rise of a liquid level in the well in response to the amount of delivery and moving the same along an internal profile line of the well by reading the control data from the storage section and driving the respective driving means. COPYRIGHT: (C)2004,JPO

9. 发明专利

JP2004028681A Head for liquid dispensing apparatus 审中-公开
公开(公告)号：JP2004028681A
公开(公告)日：2004-01-29
申请号：JP2002183062
申请日：2002-06-24
申请人： Juki Corp , Tadashi Matsunaga , ジューキ株式会社 , 松永是
发明人： UDAGAWA YUJI , NEMOTO ETSUO , MARUYAMA KOHEI , MATSUNAGA TADASHI
IPC分类号： G01N35/10
摘要： PROBLEM TO BE SOLVED: To provide a head for a liquid dispensing apparatus which overcomes a defect due to displacement of a plate. SOLUTION: The dispensing head is mounted to the liquid dispensing apparatus, equipped with a plurality of tip nozzles and has a mechanism of mounted tips for sucking or discharging a solution in the reagent plate, a movement mechanism for freely moving along a Z axis and an X or Y axis and a positioning guide for contacting a side face of the reagent plate and moving the plate to a predetermined position in a XY plane while the head vertically and downwardly moves. COPYRIGHT: (C)2004,JPO

10. 发明专利

JP2004121231A Method for detecting target nucleic acid 有权
公开(公告)号：JP2004121231A
公开(公告)日：2004-04-22
申请号：JP2003304175
申请日：2003-08-28
申请人： Juki Corp , Tadashi Matsunaga , ジューキ株式会社 , 松永是
发明人： MARUYAMA KOHEI , NEMOTO ETSUO , YODA SEI , MATSUNAGA TADASHI
IPC分类号： G01N33/53 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N33/58 , G01N37/00
摘要： PROBLEM TO BE SOLVED: To simply and efficiently detect a target nucleic acid in a sample, by clearly distinguishing the nucleic acid from another nucleic acid which has variation in one or more bases.
SOLUTION: An apparatus is so structured as to detect the target nucleic acid in a solution of the sample by distinguishing the nucleic acid from a variant nucleic acid having the variation in one or more bases, wherein the apparatus is used in conducting a hybridization method in which beads are used as a solid phase and a fluorescent or luminescent labeled probe is used as a nucleic acid probe. Further, the apparatus is so structured that a labeled probe which is not hybridized is washed and removed after hybridization reaction is completed, a solution for releasing the probe is added thereto, the target nucleic acid which is hybridized and fastened to the beads is made to be settled on a bottom surface of a container, the labeled probe is released from the beads to a supernatant by gradually raising a temperature of a reaction solution up to a temperature higher than a denaturation temperature of the target nucleic acid, and a quantity of the fluorescent light or the luminescent light is successively measured.
COPYRIGHT: (C)2004,JPO

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