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    • 3. 发明申请
    • PROCESS FOR PREPARING D-ALANINE
    • 制备D-ALANINE的方法
    • WO1988006188A1
    • 1988-08-25
    • PCT/JP1988000139
    • 1988-02-12
    • TORAY INDUSTRIES, INC.ITO, NorikoIMAMURA, ShinzoSATO, Haruyo
    • TORAY INDUSTRIES, INC.
    • C12P41/00
    • C12P13/06C12P41/001
    • A process for preparing D-alanine, which comprises culturing a particular yeast belonging to any of the genera Candida, Saccharomycopsis, Pichia, Torulopsis, Cryptococcus, Hansenula or Trichosporon and capable of digesting L-alanine and substantially not digesting D-alanine under acidic conditions using a medium containing DL-alanine as a substantially sole carbon and nitrogen source. This process permits supply of a high concentration of DL-alanine to the medium and enables D-alanine to be obtained efficiently in a high yield in a short time. After the completion of the culture, the medium contains scarcely any other organic by-products and organic impurities, thus facilitating isolation and purification of D-alanine.
    • 一种制备D-丙氨酸的方法,其包括培养属于念珠菌属,酵母菌属,毕赤酵母属,Torulopsis,隐球菌属,汉逊酵母属或Trichosporon中的任何一种的特定酵母,并且能够在酸性条件下消化L-丙氨酸并且基本上不消化D-丙氨酸 使用含有DL-丙氨酸的培养基作为基本上唯一的碳源和氮源。 该方法允许向培养基中提供高浓度的DL-丙氨酸,并能够在短时间内以高产率有效地获得D-丙氨酸。 培养完成后,培养基中几乎不含任何其他有机副产物和有机杂质,从而促进D-丙氨酸的分离和纯化。