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1. 发明授权

US08986986B2 Cell lysis device and methods of lysing cells or viruses 有权
标题翻译：细胞裂解装置和裂解细胞或病毒的方法
公开(公告)号：US08986986B2
公开(公告)日：2015-03-24
申请号：US13284364
申请日：2011-10-28
申请人： Kyu-youn Hwang , Joon-ho Kim , Sung-hong Kwon , Chin-sung Park , Hee-kyun Lim , Sun-ok Jung , Won-jong Jung
发明人： Kyu-youn Hwang , Joon-ho Kim , Sung-hong Kwon , Chin-sung Park , Hee-kyun Lim , Sun-ok Jung , Won-jong Jung
IPC分类号： C12M1/33 , C12M3/08 , C12Q1/68 , C12Q1/70 , C12M3/00 , C12M1/00
CPC分类号： C12M1/00 , C12M1/33 , C12M47/06
摘要： A method of lysing at least one of a cell and a virus, the method including: contacting a sample, which includes at least one of a cell and a virus, with a plurality of beads which are disposed in a first chamber to obtain a combination of the sample and the beads; and agitating the combination of the sample and the beads to lyse the at least one of the cell and the virus, wherein in the first chamber a liquid volume fraction is 0.6 or less, and wherein the liquid volume fraction is a value obtained by dividing a liquid volume of the first chamber by a pure void volume equivalent to a sum of the liquid volume of the first chamber and a void volume of the first chamber.
摘要翻译：一种裂解细胞和病毒中的至少一种的方法，所述方法包括：将包括细胞和病毒中的至少一种的样品与设置在第一室中的多个珠接触以获得组合的样品和珠粒; 并搅拌样品和珠的组合以裂解细胞和病毒中的至少一种，其中在第一室中液体体积分数为0.6或更小，并且其中液体体积分数是通过将第一室的液体体积相当于第一室的液体体积和第一室的空隙体积的总和的纯空隙体积。

2. 发明授权

US09044755B2 Gene analysis apparatus and gene analysis method using the same 有权
标题翻译：基因分析仪和基因分析方法采用相同的方法
公开(公告)号：US09044755B2
公开(公告)日：2015-06-02
申请号：US13176777
申请日：2011-07-06
申请人： Chin-sung Park , Won-jong Jung , Joon-ho Kim , Kak Namkoong , Kyu-youn Hwang , Won-seok Chung , Hee-kyun Lim , Sung-hong Kwon , Sun-ok Jung
发明人： Chin-sung Park , Won-jong Jung , Joon-ho Kim , Kak Namkoong , Kyu-youn Hwang , Won-seok Chung , Hee-kyun Lim , Sung-hong Kwon , Sun-ok Jung
IPC分类号： C12Q1/68 , G01N21/75 , B01L7/00 , B01L3/00
CPC分类号： B01L7/52 , B01L3/502715 , B01L2200/027 , B01L2200/028 , B01L2200/04 , B01L2200/0605 , B01L2200/0621 , B01L2200/0684 , B01L2200/10 , B01L2200/16 , B01L2300/0627 , B01L2300/0816 , B01L2300/087 , B01L2300/0874 , B01L2300/0887 , B01L2300/18 , B01L2400/0487 , B01L2400/06 , B01L2400/0655 , C12Q1/68 , G01N21/75
摘要： A gene analysis apparatus includes a sample preparation chip in which a polymerase chain reaction (“PCR”) sample is prepared, a PCR chip in which a PCR is performed on the PCR sample, and a package layer on which the sample preparation chip and the PCR chip are mounted. The package layer includes a channel through which a material flows from the sample preparation chip to the PCR chip. The sample preparation chip and the PCR chip are on a same side or on opposing sides of the package layer.
摘要翻译：基因分析装置包括准备了聚合酶链式反应（“PCR”）样品的样品制备芯片，对PCR样品进行PCR的PCR芯片，以及将样品制备芯片和 PCR芯片安装。封装层包括材料从样品制备芯片流向PCR芯片的通道。样品制备芯片和PCR芯片在封装层的相同侧或相对侧上。

3. 发明授权

US08895297B2 Micro-device and methods for disrupting cells 有权
标题翻译：用于破坏细胞的微型装置和方法
公开(公告)号：US08895297B2
公开(公告)日：2014-11-25
申请号：US13492179
申请日：2012-06-08
申请人： Kyu-youn Hwang , Joon-ho Kim , Sung-hong Kwon , Chin-sung Park , Hee-kyun Lim , Sun-ok Jung , Won-jong Jung
发明人： Kyu-youn Hwang , Joon-ho Kim , Sung-hong Kwon , Chin-sung Park , Hee-kyun Lim , Sun-ok Jung , Won-jong Jung
IPC分类号： C12N1/06 , C12M1/00 , B01L3/00 , C12M1/33 , C12M3/08 , C12M3/00
CPC分类号： C12M47/06 , B01L3/502753 , B01L2300/0816 , B01L2300/0867 , B01L2400/0481 , C12N1/06
摘要： A micro-device for disrupting cells includes a first chamber in which the cells are disrupted, a second chamber which is pressurized and depressurized, a flexible membrane which separates the first chamber and the second chamber and is vibrated by pressuring and depressurizing the second chamber, and a micro-unit confined in the first chamber, where the micro-unit disrupts the cells in the first chamber.
摘要翻译：用于破坏电池的微型装置包括其中电池被破坏的第一室，被加压和减压的第二室，分离第一室和第二室的柔性膜，并且通过对第二室进行加压和减压而振动，以及限制在第一室中的微单元，其中微单元破坏第一室中的单元。

4. 发明授权

US08580506B2 Method and composition for enhancing efficiency and sensitivity in polymerase chain reaction 有权
标题翻译：用于提高聚合酶链反应的效率和灵敏度的方法和组合物
公开(公告)号：US08580506B2
公开(公告)日：2013-11-12
申请号：US13069969
申请日：2011-03-23
申请人： Sun-ok Jung , Hee-kyun Lim , Kyu-youn Hwang , Joon-ho Kim , Sung-hong Kwon
发明人： Sun-ok Jung , Hee-kyun Lim , Kyu-youn Hwang , Joon-ho Kim , Sung-hong Kwon
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6806 , C12Q1/686 , C12Q2527/125
摘要： Methods and compositions for enhancing reaction efficiency and sensitivity in polymerase chain reaction (PCR) are disclosed.
摘要翻译：公开了用于提高聚合酶链反应（PCR）中的反应效率和灵敏度的方法和组合物。

5. 发明授权

US08859272B2 Micro-device and methods for disrupting cells 有权
标题翻译：用于破坏细胞的微型装置和方法
公开(公告)号：US08859272B2
公开(公告)日：2014-10-14
申请号：US13275871
申请日：2011-10-18
申请人： Kyu-youn Hwang , Joon-ho Kim , Sung-hong Kwon , Chin-sung Park , Hee-kyun Lim , Sun-ok Jung , Won-jong Jung
发明人： Kyu-youn Hwang , Joon-ho Kim , Sung-hong Kwon , Chin-sung Park , Hee-kyun Lim , Sun-ok Jung , Won-jong Jung
IPC分类号： C12M1/33 , C12Q1/68
CPC分类号： C12Q1/6806 , C12M1/33 , C12M47/06
摘要： A micro-device for disrupting cells includes a first chamber in which the cells are disrupted, a second chamber which is pressurized and depressurized, a flexible membrane which separates the first chamber and the second chamber and is vibrated by pressuring and depressurizing the second chamber, and a micro-unit confined in the first chamber, where the micro-unit disrupts the cells in the first chamber
摘要翻译：用于破坏电池的微型装置包括其中电池被破坏的第一室，被加压和减压的第二室，分离第一室和第二室的柔性膜，并且通过对第二室进行加压和减压而振动，以及限制在第一室中的微单元，其中微单元破坏第一室中的单元

6. 发明授权

US07803539B2 Method of isolating and purifying nucleic acids using immobilized hydrogel or PEG-hydrogel copolymer 失效
标题翻译：使用固定化水凝胶或PEG-水凝胶共聚物分离和纯化核酸的方法
公开(公告)号：US07803539B2
公开(公告)日：2010-09-28
申请号：US11297783
申请日：2005-12-07
申请人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Jun-hong Min
发明人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Jun-hong Min
IPC分类号： C12Q1/68 , C12M1/34 , C07H21/00
CPC分类号： C07H21/04
摘要： Provided is a method of isolating and purifying nucleic acids using an immobilized hydrogel or polyethylene glycol (PEG)-hydrogel copolymer. The method includes: immobilizing a functional group-containing hydrogel or PEG-hydrogel copolymer on a substrate; adding a mixed sample solution containing a salt and nucleic acids to the hydrogel- or PEG-hydrogel copolymer-immobilized substrate to bind the nucleic acids to the hydrogel or the PEG-hydrogel copolymer; washing the nucleic acid-bound hydrogel or PEG-hydrogel copolymer; and eluting the nucleic acids from the hydrogel or the PEG-hydrogel copolymer using an elution solvent. Therefore, binding and elution of nucleic acids can be performed even with no addition of a separate chemical substance, and an effect on a subsequent process such as PCR can be minimized. Furthermore, the amount and intensity for binding nucleic acids can be adjusted according to PEG concentration, and the presence of a hydrogel compound on a substrate enables patterning.
摘要翻译：提供了使用固定的水凝胶或聚乙二醇（PEG） - 水凝胶共聚物来分离和纯化核酸的方法。该方法包括：将含官能团的水凝胶或PEG-水凝胶共聚物固定在基材上; 将含有盐和核酸的混合样品溶液加入到水凝胶或PEG-水凝胶共聚物固定的底物中，以将核酸与水凝胶或PEG-水凝胶共聚物结合; 洗涤核酸结合的水凝胶或PEG-水凝胶共聚物; 并使用洗脱溶剂从水凝胶或PEG-水凝胶共聚物中洗脱核酸。因此，即使没有添加单独的化学物质也可以进行核酸的结合和洗脱，并且可以最小化对后续过程（例如PCR）的影响。此外，可以根据PEG浓度调节结合核酸的量和强度，并且在基材上存在水凝胶化合物能够进行图案化。

7. 发明申请

US20060099711A1 Cell lysis method by immobilized metal-ligand complex 失效
标题翻译：固定化金属配体复合物的细胞裂解法
公开(公告)号：US20060099711A1
公开(公告)日：2006-05-11
申请号：US11267005
申请日：2005-11-04
申请人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Soo-min Ma , Nam Huh , Soo-suk Lee
发明人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Soo-min Ma , Nam Huh , Soo-suk Lee
IPC分类号： G01N1/00
CPC分类号： C12Q1/6806 , Y10T436/25 , C12Q2523/308
摘要： Provided is a lysis method for cells or viruses, including: immobilizing a metal-ligand complex on a solid support; and mixing the complex immobilized on the support with a cell or virus solution. According to the lysis method, by immobilizing a chemical on a solid support to perform cell lysis, the dilution problem according to the addition of a cell lysis solution can be resolved and a separate process of removing the chemical is not required so as to reduce the steps upon LOC implementation. In addition, since a variety of solid supports, such as chips, beads, nanoparticles etc. can be used, cell lysis apparatuses of various forms can be fabricated.
摘要翻译：提供了一种细胞或病毒的裂解方法，包括：将固定支持物上的金属 - 配体复合物固定; 并将固定在载体上的复合物与细胞或病毒溶液混合。根据裂解方法，通过将化学物质固定在固体支持体上以进行细胞裂解，可以解决根据细胞溶解溶液的添加的稀释问题，并且不需要单独的除去化学品的方法，以减少 LOC实施步骤。此外，由于可以使用各种固体支持体，例如芯片，珠粒，纳米粒子等，因此可以制造各种形式的细胞裂解装置。

8. 发明授权

US07648838B2 Cell lysis method by immobilized metal-ligand complex 失效
标题翻译：固定化金属配体复合物的细胞裂解法
公开(公告)号：US07648838B2
公开(公告)日：2010-01-19
申请号：US11267005
申请日：2005-11-04
申请人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Soo-min Ma , Nam Huh , Soo-suk Lee
发明人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Soo-min Ma , Nam Huh , Soo-suk Lee
IPC分类号： G01N33/48 , G01N33/20 , G01N1/00
CPC分类号： C12Q1/6806 , Y10T436/25 , C12Q2523/308
摘要： Provided is a lysis method for cells or viruses, including: immobilizing a metal-ligand complex on a solid support; and mixing the complex immobilized on the support with a cell or virus solution. According to the lysis method, by immobilizing a chemical on a solid support to perform cell lysis, the dilution problem according to the addition of a cell lysis solution can be resolved and a separate process of removing the chemical is not required so as to reduce the steps upon LOC implementation. In addition, since a variety of solid supports, such as chips, beads, nanoparticles etc. can be used, cell lysis apparatuses of various forms can be fabricated.
摘要翻译：提供了一种细胞或病毒的裂解方法，包括：将固定支持物上的金属 - 配体复合物固定; 并将固定在载体上的复合物与细胞或病毒溶液混合。根据裂解方法，通过将化学物质固定在固体支持体上以进行细胞裂解，可以解决根据细胞溶解溶液的添加的稀释问题，并且不需要单独的除去化学品的方法，以减少 LOC实施步骤。此外，由于可以使用各种固体支持物，例如芯片，珠粒，纳米粒子等，因此可以制造各种形式的细胞裂解装置。

9. 发明申请

US20060270031A1 Cell separation method and kit using phase separation 审中-公开
标题翻译：细胞分离方法和试剂盒使用相分离
公开(公告)号：US20060270031A1
公开(公告)日：2006-11-30
申请号：US11439916
申请日：2006-05-24
申请人： Kyu-youn Hwang , Hee-kyun Lim , Joon-ho Kim
发明人： Kyu-youn Hwang , Hee-kyun Lim , Joon-ho Kim
IPC分类号： C12N7/00 , C12N5/00 , C12N7/01 , C12N5/02
CPC分类号： C12N1/02 , C12N7/00 , C12N2795/00051
摘要： A method of separating cells or viruses from a mixture using phase separation is provided. The method includes: suspending a sample containing cells or viruses in a kosmotropic salt solution having a pH of 3-6; aggregating the cells or viruses by adding a cationic polymer to the suspension; adhering the cells or viruses to a solid substrate with the aid of phase separation of polymer by placing the suspension in contact with the solid substrate; and separating the solid substrate on which the cells or viruses are adhered from the suspension. Accordingly, it is possible to separate a high concentration of cells, even when using a flow control system. In addition, the method can be conveniently applied to lab-on-a-chip technology.
摘要翻译：提供了使用相分离从混合物中分离细胞或病毒的方法。该方法包括：将含有细胞或病毒的样品悬浮在pH为3-6的去离子水溶液中; 通过向悬浮液中加入阳离子聚合物来聚集细胞或病毒; 借助于通过将悬浮液与固体基质接触来使聚合物相分离，将细胞或病毒粘附到固体基质上; 并从悬浮液中分离其上粘附细胞或病毒的固体底物。因此，即使使用流量控制系统，也可以分离高浓度的电池。此外，该方法可以方便地应用于片上实验室技术。

10. 发明申请

US20060134675A1 Method of isolating and purifying nucleic acids using immobilized hydrogel or PEG-hydrogel copolymer 失效
标题翻译：使用固定化水凝胶或PEG-水凝胶共聚物分离和纯化核酸的方法
公开(公告)号：US20060134675A1
公开(公告)日：2006-06-22
申请号：US11297783
申请日：2005-12-07
申请人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Jun-hong Min
发明人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Jun-hong Min
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C07H21/04
摘要： Provided is a method of isolating and purifying nucleic acids using an immobilized hydrogel or polyethylene glycol (PEG)-hydrogel copolymer. The method includes: immobilizing a functional group-containing hydrogel or PEG-hydrogel copolymer on a substrate; adding a mixed sample solution containing a salt and nucleic acids to the hydrogel- or PEG-hydrogel copolymer-immobilized substrate to bind the nucleic acids to the hydrogel or the PEG-hydrogel copolymer; washing the nucleic acid-bound hydrogel or PEG-hydrogel copolymer; and eluting the nucleic acids from the hydrogel or the PEG-hydrogel copolymer using an elution solvent. Therefore, binding and elution of nucleic acids can be performed even with no addition of a separate chemical substance, and an effect on a subsequent process such as PCR can be minimized. Furthermore, the amount and intensity for binding nucleic acids can be adjusted according to PEG concentration, and the presence of a hydrogel compound on a substrate enables patterning.
摘要翻译：提供了使用固定的水凝胶或聚乙二醇（PEG） - 水凝胶共聚物来分离和纯化核酸的方法。该方法包括：将含官能团的水凝胶或PEG-水凝胶共聚物固定在基材上; 将含有盐和核酸的混合样品溶液加入到水凝胶或PEG-水凝胶共聚物固定的底物中，以将核酸与水凝胶或PEG-水凝胶共聚物结合; 洗涤核酸结合的水凝胶或PEG-水凝胶共聚物; 并使用洗脱溶剂从水凝胶或PEG-水凝胶共聚物中洗脱核酸。因此，即使没有添加单独的化学物质也可以进行核酸的结合和洗脱，并且可以最小化对后续过程（例如PCR）的影响。此外，可以根据PEG浓度调节结合核酸的量和强度，并且在基材上存在水凝胶化合物能够进行图案化。

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