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1. 发明授权

US4940668A Method for increasing agglutination of groups of cells to produce improved cell layer interface in centrifuged blood sample 失效
标题翻译：增加细胞组凝集以在离心血液样品中产生改善的细胞层界面的方法
公开(公告)号：US4940668A
公开(公告)日：1990-07-10
申请号：US98370
申请日：1987-09-18
申请人： Stephen C. Wardlaw , Robert A. Levine , Rodolfo R. Rodriguez , Michael R. Loken
发明人： Stephen C. Wardlaw , Robert A. Levine , Rodolfo R. Rodriguez , Michael R. Loken
IPC分类号： G01N33/49 , G01N33/50 , G01N33/80
CPC分类号： G01N33/80 , G01N33/491 , G01N33/5002 , Y10T436/107497 , Y10T436/25 , Y10T436/25375
摘要： In order to produce a more well defined interface between adjacent cell layers in a centrifuged sample of anticoagulated whole blood, a material which will bond one group of cells together is added to the blood sample prior to centrifugation. The bonding material must produce a high strength bond between one group of cells, but not effect the other cell types. The material is added prior to centrifugation of the blood sample.
摘要翻译：为了在离心的抗凝全血样品中产生相邻细胞层之间更加界定的界面，将离心之前将一组细胞结合在一起的物质加入到血液样品中。接合材料必须在一组电池之间产生高强度的结合，但不影响其他电池类型。在血液样品离心之前加入材料。

2. 发明授权

US4695553A Method for increasing agglutination of groups of cells to produce improved cell layer interface in centrifuged blood sample using antibodies 失效
标题翻译：增加细胞组凝集的方法，以使用抗体在离心血液样品中产生改善的细胞层界面
公开(公告)号：US4695553A
公开(公告)日：1987-09-22
申请号：US794127
申请日：1985-11-01
申请人： Stephen C. Wardlaw , Robert A. Levine , Rodolfo R. Rodriguez , Michael R. Loken
发明人： Stephen C. Wardlaw , Robert A. Levine , Rodolfo R. Rodriguez , Michael R. Loken
IPC分类号： G01N33/53 , C12Q1/00 , G01N33/48 , G01N33/49 , G01N33/50 , G01N33/80 , G01N1/18
CPC分类号： G01N33/5002 , G01N33/491 , G01N33/80 , Y10T436/25375
摘要： In order to produce a more well defined interface between adjacent cell layers in a centrifuged sample of anticoagulated whole blood, a material which will bond one group of cells together is added to the blood sample prior to centrifugation. The bonding material must produce a high strength bond between one group of cells, but not effect the other cell types. The material is added prior to centrifugation of the blood sample. An example of a bonding agent is a monoclonal antibody.
摘要翻译：为了在离心的抗凝全血样品中产生相邻细胞层之间更加界定的界面，将离心之前将一组细胞结合在一起的物质加入到血液样品中。接合材料必须在一组电池之间产生高强度的结合，但不影响其他电池类型。在血液样品离心之前加入材料。粘合剂的实例是单克隆抗体。

3. 发明授权

US5047321A Method for analysis of cellular components of a fluid 失效
标题翻译：分析流体细胞成分的方法
公开(公告)号：US5047321A
公开(公告)日：1991-09-10
申请号：US207099
申请日：1988-06-15
申请人： Michael R. Loken , Leon W. M. M. Terstappen
发明人： Michael R. Loken , Leon W. M. M. Terstappen
IPC分类号： G01N33/48 , A61B5/145 , A61B5/1459 , G01N15/14 , G01N33/49 , G01N33/53 , G01N33/569 , G01N33/574 , G01N33/58
CPC分类号： G01N33/56972 , G01N15/1459 , G01N33/56977 , G01N33/57492 , G01N2015/1006 , G01N2015/1402 , G01N2333/705 , Y10S435/973 , Y10S436/80 , Y10S436/805 , Y10T436/143333
摘要： A method for multi-parameter analysis of cells in a body fluid sample is described which makes use of a plurality of fluorescence measurements, comprising at least two nucleic acid dyes and at least one fluorescently labelled cell surface marker, and a plurality of light scattering measurements. A kit containing the nucleic acid dyes and cell surface marker also is described.

4. 发明授权

US4885237A Detection of cellular DNA 失效
标题翻译：检测细胞DNA
公开(公告)号：US4885237A
公开(公告)日：1989-12-05
申请号：US205013
申请日：1988-06-06
申请人： Elizabeth L. Evans , Nick J. Gonchoroff , Philip R. Greipp , David W. Houck , Jerry A. Katzmann , Robert A. Kyle , Michael R. Loken
发明人： Elizabeth L. Evans , Nick J. Gonchoroff , Philip R. Greipp , David W. Houck , Jerry A. Katzmann , Robert A. Kyle , Michael R. Loken
IPC分类号： C12Q1/68 , G01N33/53
CPC分类号： C12Q1/68 , G01N33/5308 , Y10S435/81 , Y10S436/822 , Y10S530/808
摘要： A method is described for the detection of halodeoxyuridines taken up by cells during DNA synthesis by the use of monoclonal antibodies. The described method also allows the simultaneous detection of cell surface receptors.
摘要翻译：描述了通过使用单克隆抗体在DNA合成期间检测由细胞摄取的卤代脱氧尿苷的方法。所述方法还允许同时检测细胞表面受体。

5. 发明授权

US5840580A Phenotypic characterization of the hematopoietic stem cell 失效
标题翻译：造血干细胞的表型表征
公开(公告)号：US5840580A
公开(公告)日：1998-11-24
申请号：US856406
申请日：1997-05-14
申请人： Leon W. Terstappen , Michael R. Loken , Shiang Huang , Johanna Olweus , Fridtjof Lund-Johansen
发明人： Leon W. Terstappen , Michael R. Loken , Shiang Huang , Johanna Olweus , Fridtjof Lund-Johansen
IPC分类号： A61K35/14 , A61K35/28 , A61K48/00 , C12N5/0789 , A01N63/00 , C12N5/06 , C12N5/08
CPC分类号： C12N5/0647 , A61K35/28 , A61K48/00
摘要： One or more population of cells enriched for human hematopoietic stem cells is disclosed. HSC in this population of cells are capable of limited self-renewal and are capable of differentiating into all elements of the hematopoietic system. This population of cells has the phenotype of CD34.sup.+ /CD38.sup.- and more preferably CD34.sup.+ /CD38.sup.- /HLA-DR.sup.+. Cells within this population have been found to express CD13, CD33 and CD71. Hematopoietic stem cells can be used in a number of therapies, including autologous transplantation and in gene therapy.
摘要翻译：公开了一种或多种富含人造血干细胞的细胞群。 HSC在这个细胞群中能够有限的自我更新，并能够区分成造血系统的所有元素。这种细胞群具有CD34 + / CD38-，更优选CD34 + / CD38- / HLA-DR +的表型。已经发现该群体内的细胞表达CD13，CD33和CD71。造血干细胞可用于许多疗法，包括自体移植和基因治疗。

6. 发明授权

US5224058A Method for data transformation 失效
标题翻译：数据转换方法
公开(公告)号：US5224058A
公开(公告)日：1993-06-29
申请号：US517096
申请日：1990-05-01
申请人： Ronald A. Mickaels , Richard K. Dost , Leon W. M. M. Terstappen , Michael R. Loken
发明人： Ronald A. Mickaels , Richard K. Dost , Leon W. M. M. Terstappen , Michael R. Loken
IPC分类号： G01N15/14 , G01N21/47 , G01N33/48 , G01N33/49 , G01N33/536
CPC分类号： G01N15/1456 , G01N2015/1477
摘要： A method for the non-linear, non-logrithmic transformation of one or more parameters of data gathered by means of flow cytometry is disclosed wherein the method seeks to maximize the resolution between populations of interest for each parameter while maintaining the dynamic range of the data recorded.

7. 发明授权

US5137809A Method to determine the composition of bone marrow samples 失效
标题翻译：确定骨质疏松样品组成的方法
公开(公告)号：US5137809A
公开(公告)日：1992-08-11
申请号：US572303
申请日：1990-07-19
申请人： Michael R. Loken , Curt I. Civin , Virendra O. Shah
发明人： Michael R. Loken , Curt I. Civin , Virendra O. Shah
IPC分类号： G01N33/577 , G01N33/483 , G01N33/53 , G01N33/543 , G01N33/569
CPC分类号： G01N33/56972 , G01N15/1459 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , Y10S436/80
摘要： A method and kit for identification of the lineages and stages of hematopoietic cells in a sample wherein the cells are treated with labeled monoclonal antibodies selected to distinguish between each lineage and maturational stages within each lineage.

8. 发明授权

US5057413A Method for discriminating between intact and damaged cells in a sample 失效
标题翻译：用于在样品中鉴别和破坏细胞之间的方法
公开(公告)号：US5057413A
公开(公告)日：1991-10-15
申请号：US206454
申请日：1988-06-13
申请人： Leon W. M. M. Terstappen , Michael R. Loken , Virendra O. Shah
发明人： Leon W. M. M. Terstappen , Michael R. Loken , Virendra O. Shah
IPC分类号： G01N33/48 , A61B10/00 , C12Q1/02 , G01N15/14 , G01N21/64 , G01N33/53 , G01N33/543 , G01N33/569
CPC分类号： C12Q1/02 , G01N15/1459 , G01N21/6428 , G01N33/56972 , G01N15/14 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , G01N2021/6439 , Y10S436/80
摘要： A method is disclosed for the discrimination between intact and damaged cells in a sample, and more particularly, the method uses a vital nucleic acid dye to selectively stain intact versus damaged cells which then may be counted and sorted by flow cytometry means. The method also may be used in conjunction with fluorescently labelled monoclonal antibodies to simultaneously identify cellular antigens.

9. 发明授权

US4867908A Method and materials for calibrating flow cytometers and other analysis instruments 失效
标题翻译：用于校准流式细胞仪和其他分析仪器的方法和材料
公开(公告)号：US4867908A
公开(公告)日：1989-09-19
申请号：US58206
申请日：1987-06-04
申请人： Diether J. Recktenwald , Rickie S. Kerndt , Michael R. Loken , Chia H. Chen
发明人： Diether J. Recktenwald , Rickie S. Kerndt , Michael R. Loken , Chia H. Chen
IPC分类号： G01N15/10 , G01N15/14
CPC分类号： G01N15/1012 , G01N15/1456 , G01N2015/1006 , G01N2015/1018 , G01N2015/1477 , Y10T436/10 , Y10T436/101666 , Y10T436/105831
摘要： A method for calibrating an instrument for using that instrument to obtain at least one light-related signal from particles under analysis comprises directing an incident beam of light at calibration particles having one or more known characteristics related to the particles expected to be analyzed. Both a light signal and a noise signal from the calibration particles are detected. A measurement is made of the ratio of the detected light signal to the detected noise signal, and that measurement is reported. The measured ratio is then compared to a predetermined ratio which represents a threshold for minimum instrument performance. This method further includes adjusting, if the predetermined ratio has not been attained, the operation of the instrument, while the calibration particles are within the incident beam of light, until the measured ratio reaches the predetermined ratio whereby the instrument is calibrated for subsequently obtaining the light signal from particles to be analyzed. A kit of reagents for use in calibrating an instrument for performing two-color analysis of particles and particle standars for calibrating fluorescence analysis instruments are also part of the instant invention.
摘要翻译：用于校准用于使用该仪器以获得来自待分析的颗粒的至少一种光相关信号的仪器的方法包括将入射光束引导到具有与预期分析的颗粒相关的一个或多个已知特征的校准颗粒。检测来自校准颗粒的光信号和噪声信号。测量检测到的光信号与检测到的噪声信号的比率，并且报告测量。然后将测量的比率与表示用于最小仪器性能的阈值的预定比率进行比较。该方法还包括如果没有达到预定比例，则在校准颗粒在入射光束内时调节仪器的操作，直到所测量的比率达到预定比率，由此仪器被校准以便随后获得来自待分析颗粒的光信号。用于校准用于进行用于校准荧光分析仪器的颗粒和颗粒标准物的双色分析的仪器的试剂盒也是本发明的一部分。

10. 发明授权

US08630833B2 System, method, and article for detecting abnormal cells using multi-dimensional analysis 有权
标题翻译：使用多维分析检测异常细胞的系统，方法和文章
公开(公告)号：US08630833B2
公开(公告)日：2014-01-14
申请号：US11357359
申请日：2006-02-17
申请人： Michael R. Loken , Sanjaya N. Joshi
发明人： Michael R. Loken , Sanjaya N. Joshi
IPC分类号： G06G7/48 , G01N33/48
CPC分类号： G01N33/5091 , G01N15/1456 , G01N33/56966 , G01N33/56972 , G01N33/574 , G01N33/57492 , G01N2015/0065 , G06F19/20 , G06F19/24 , G06F19/26 , G06K9/00147 , G06K9/6218 , G06K9/6254
摘要： A system, method, and article for diagnosing a test set of biological cells. For example, in one embodiment a normal set of cells is characterized using flow cytometry. A centroid and radius are defined for a set of clusters in an n-dimensional space corresponding to a normal maturation for a cell lineage in the normal set of cells. A test set of cells is characterized using flow cytometry and the characterization is compared to the set of clusters.
摘要翻译：用于诊断生物细胞测试集的系统，方法和文章。例如，在一个实施方案中，使用流式细胞术来表征正常的细胞集合。对于在正常细胞集合中的细胞谱系的正常成熟对应的n维空间中的一组聚类，定义了质心和半径。使用流式细胞术表征细胞的测试组，并将表征与集合集进行比较。

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