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1. 发明授权

US06960456B1 Method for preparing conjugated linoleic acid 失效
标题翻译：共轭亚油酸的制备方法
公开(公告)号：US06960456B1
公开(公告)日：2005-11-01
申请号：US10130426
申请日：2000-11-16
申请人： Simo Laakso , Auli Rainio , Marjatta Vahvaselka , Annika Mäyrä-Mäkinen , Soile Tynkkynen , Tarja Suomalainen
发明人： Simo Laakso , Auli Rainio , Marjatta Vahvaselka , Annika Mäyrä-Mäkinen , Soile Tynkkynen , Tarja Suomalainen
IPC分类号： C12P7/40 , C12P7/64 , C12R1/01
CPC分类号： C12P7/6427 , C12P7/6472
摘要： The invention relates to a method for preparing conjugated linoleic acid and particularly its cis-9,trans-11 isomer from linoleic acid by fermentation. The invention also relates to products prepared by the method.
摘要翻译：本发明涉及通过发酵从亚油酸制备共轭亚油酸，特别是其顺式-9反式-11异构体的方法。本发明还涉及通过该方法制备的产品。

2. 发明申请

US20090131700A1 Production of fatty acid and fatty acid ester 失效
标题翻译：生产脂肪酸和脂肪酸酯
公开(公告)号：US20090131700A1
公开(公告)日：2009-05-21
申请号：US12231975
申请日：2008-09-08
申请人： Ossi Pastinen , Simo Laakso , Sanna Hokkanen , Marjatta Vahvaselka
发明人： Ossi Pastinen , Simo Laakso , Sanna Hokkanen , Marjatta Vahvaselka
IPC分类号： C11B1/00
CPC分类号： C11C3/003 , C10G2300/1011 , C10L1/026 , C11C1/025 , Y02E50/13 , Y02P30/20
摘要： The present invention concerns a process for forming a fatty acid, a fatty acid ester or a mixture thereof from a soap-comprising starting material, in which process a metal-ion forming agent is added to the starting material, whereby a mixture is formed, which contains an insoluble phase and a liquid phase, the insoluble phase is separated from the liquid phase, and an acid is added into the insoluble phase to form a fatty acid, or a monohydric alcohol and an acid catalyst are added to form a fatty acid ester, whereby two phases are formed, an aqueous phase and an organic phase, or, first, an acid is added and then a monohydric alcohol and an acid catalyst are added into at least a portion of the formed fatty acid to esterify the fatty acid.
摘要翻译：本发明涉及一种由含皂原料形成脂肪酸，脂肪酸酯或其混合物的方法，其中向原料中加入金属离子形成剂，从而形成混合物，其含有不溶相和液相，将不溶相与液相分离，并将酸加入到不溶相中以形成脂肪酸，或加入一元醇和酸催化剂以形成脂肪酸酯，由此形成两相，水相和有机相，或者首先加入酸，然后将一元醇和酸催化剂加入到形成的脂肪酸的至少一部分中以酯化脂肪酸。

3. 发明申请

US20090197312A1 Production of fat from alcohol 审中-公开
标题翻译：从酒精生产脂肪
公开(公告)号：US20090197312A1
公开(公告)日：2009-08-06
申请号：US12231956
申请日：2008-09-08
申请人： Ossi Pastinen , Simo Laakso , Sanna Hokkanen , Marjatta Vahvaselka
发明人： Ossi Pastinen , Simo Laakso , Sanna Hokkanen , Marjatta Vahvaselka
IPC分类号： C12P7/64 , C07C67/00
CPC分类号： C12P7/649 , C12P7/6463 , Y02E50/13
摘要： The present invention concerns a process for forming a lipid or a mixture of lipids from a starting material, which comprises at least one alcohol and a soap or a soap precursor, the process comprising adding a metal-ion forming agent to the starting material, whereby a mixture is formed, which contains an insoluble phase and a liquid phase, separating the insoluble phase from the liquid phase, contacting the lipid-producing microorganism with the liquid phase on a culturing substrate, whereby the microorganism cells begin producing lipid, and collecting the lipids. The present invention also concerns a process for forming a lipid or a mixture of lipids from an alcohol-containing liquid phase, which comprises at least one alcohol.
摘要翻译：本发明涉及从起始原料形成脂质或脂质混合物的方法，该方法包括至少一种醇和皂或皂前体，该方法包括向起始原料中加入金属离子形成剂，由此形成含有不溶相和液相的混合物，将不溶相与液相分离，使培养基上的脂质产生微生物与液相接触，由此微生物细胞开始产生脂质，并收集脂质本发明还涉及由含有至少一种醇的含醇液相形成脂质或脂质混合物的方法。

4. 发明申请

US20050170477A1 Process for preparing conjugated linoleic acid 审中-公开
标题翻译：共轭亚油酸的制备方法
公开(公告)号：US20050170477A1
公开(公告)日：2005-08-04
申请号：US10508987
申请日：2003-03-27
申请人： Marjatta Vahvaselka , Pekka Lethnen , Simo Laakso
发明人： Marjatta Vahvaselka , Pekka Lethnen , Simo Laakso
IPC分类号： C12N1/20 , C12P7/64 , C12R1/01 , C12N1/21
CPC分类号： C12P7/6427
摘要： The invention relates to a process for preparing conjugated linoleic acid. In particular, the invention discloses a process for preparing conjugated linoleic acid, particularly the cis-9,trans-11 isomer thereof from grain by means of beneficial bacteria. The invention also relates to the products prepared by the process.
摘要翻译：本发明涉及一种制备共轭亚油酸的方法。特别地，本发明公开了通过有益细菌从谷物制备共轭亚油酸，特别是其顺式-9反式-11异构体的方法。本发明还涉及通过该方法制备的产品。

5. 发明授权

US08900832B2 Method for the production of fat 有权
标题翻译：生产脂肪的方法
公开(公告)号：US08900832B2
公开(公告)日：2014-12-02
申请号：US12803350
申请日：2010-06-24
申请人： Heidi Kahelin , Simo Laakso , Ossi Pastinen , Miia Mujunen , Tarja Suomalainen
发明人： Heidi Kahelin , Simo Laakso , Ossi Pastinen , Miia Mujunen , Tarja Suomalainen
IPC分类号： C12M1/00
CPC分类号： C12P7/6463 , A23K20/158 , A23L33/115 , C11B1/025 , C11B1/10 , C11C3/003 , Y02E50/13
摘要： The present invention relates to a method for the production of fat with a principal application as transportation biofuel or a component or raw material therefor. According to the method, cell masses, cell suspensions and/or liquid phases formed in the production of single cell oil, and/or biomass-containing side streams or microorganism cell masses for another purpose and/or originating from other sources, are contacted with a fat-production capable microorganism and the organism is allowed to produce fat. The resulting fat is recovered or the microorganism mass is passed to a single-cell oil production process. By means of the invention, the organic matter present in the cell mass and side streams of single-cell oil can be re-utilized for the production of the single-cell oil, thereby improving a total fat yield, as well as reducing an organic load of the side streams.
摘要翻译：本发明涉及作为运输生物燃料或其成分或原料的主要应用的脂肪生产方法。根据该方法，在单细胞油生产中形成的细胞团，细胞悬浮液和/或液相，和/或用于另一目的和/或源自其它来源的含生物质的侧流或微生物细胞块与生产能力的微生物和生物被允许产生脂肪。将所得脂肪回收或将微生物物质转移至单细胞油生产过程。通过本发明，存在于单细胞油的细胞质量和侧流中的有机物质可以再利用于单细胞油的生产，从而提高总脂肪产量，以及还原有机物负载侧流。

6. 发明申请

US20110065940A1 Method for the production of fat 有权
标题翻译：生产脂肪的方法
公开(公告)号：US20110065940A1
公开(公告)日：2011-03-17
申请号：US12803350
申请日：2010-06-24
申请人： Heidi Kahelin , Simo Laakso , Ossi Pastinen , Miia Mujunen , Tarja Suomalainen
发明人： Heidi Kahelin , Simo Laakso , Ossi Pastinen , Miia Mujunen , Tarja Suomalainen
IPC分类号： C12P7/64 , C07C53/00 , C12N1/16 , C12N1/18 , C12N1/14
CPC分类号： C12P7/6463 , A23K20/158 , A23L33/115 , C11B1/025 , C11B1/10 , C11C3/003 , Y02E50/13
摘要： The present invention relates to a method for the production of fat with a principal application as transportation biofuel or a component or raw material therefor. According to the method, cell masses, cell suspensions and/or liquid phases formed in the production of single cell oil, and/or biomass-containing side streams or microorganism cell masses for another purpose and/or originating from other sources, are contacted with a fat-production capable microorganism and the organism is allowed to produce fat. The resulting fat is recovered or the microorganism mass is passed to a single-cell oil production process. By means of the invention, the organic matter present in the cell mass and side streams of single-cell oil can be re-utilized for the production of the single-cell oil, thereby improving a total fat yield, as well as reducing an organic load of the side streams.
摘要翻译：本发明涉及作为运输生物燃料或其成分或原料的主要应用的脂肪生产方法。根据该方法，在单细胞油生产中形成的细胞团，细胞悬浮液和/或液相，和/或用于另一目的和/或源自其它来源的含生物质的侧流或微生物细胞块与生产能力的微生物和生物被允许产生脂肪。将所得脂肪回收或将微生物物质转移至单细胞油生产过程。通过本发明，存在于单细胞油的细胞质量和侧流中的有机物质可以再利用于单细胞油的生产，从而提高总脂肪产量，以及还原有机物负载侧流。

7. 发明申请

US20120159838A1 Process for Producing Enzymes 有权
标题翻译：生产酶的方法
公开(公告)号：US20120159838A1
公开(公告)日：2012-06-28
申请号：US13333188
申请日：2011-12-21
申请人： Annika Malm , Simo Laakso , Ossi Pastinen , Heidi Kahelin , Miia Mujunen
发明人： Annika Malm , Simo Laakso , Ossi Pastinen , Heidi Kahelin , Miia Mujunen
IPC分类号： C10L1/18 , C12N9/14 , C12N9/02 , C10L1/00 , C12N9/90 , C12N9/00 , C12N9/24 , C12N9/88 , C12N9/10
CPC分类号： C12N9/00 , C12N9/2437 , C12N9/248 , C12P7/6463 , Y02E50/13 , Y02E50/16 , Y02E50/17 , Y02P20/582
摘要： The present invention relates to a process for producing enzymes and single cell oil. The process comprises that microorganisms capable of producing both single cell oil and enzymes are cultivated under conditions suitable for single cell oil production and enzyme production in a single cell oil production process. A microorganism culture comprising single cell oil and enzymes is obtained and at least part of the microorganism culture, of the supernatant and/or microorganism cells separated from the microorganism culture, of protein fraction enriched from the supernatant, and/or of protein fraction obtained from the cells is used as an enzyme preparation or as a source of enzymes. Single cell oil is recovered from the microorganism cells and used as biofuel, component of biofuel or as a starting material for biofuel production. Enzymes produced according to the process are used in the same or in another industrial process.
摘要翻译：本发明涉及一种生产酶和单细胞油的方法。该方法包括在单细胞油生产过程中，能够产生单细胞油和酶的微生物在适于单细胞油生产和酶生产的条件下培养。获得包含单细胞油和酶的微生物培养物，并且从微生物培养物分离的上清液和/或微生物细胞的微生物培养物的至少一部分，富含上清液的蛋白质级分和/或从上清液获得的蛋白质级分细胞被用作酶制剂或酶的来源。单细胞油从微生物细胞中回收并用作生物燃料，生物燃料的组分或作为生物燃料生产的起始材料。根据该方法生产的酶在相同或另一工业过程中使用。

8. 发明申请

US20120220509A1 Oil Recovery Method 有权
标题翻译：油回收方法
公开(公告)号：US20120220509A1
公开(公告)日：2012-08-30
申请号：US13399186
申请日：2012-02-17
申请人： Miia Mujunen , Ossi Pastinen , Ilkka Lehtomaki , Simo Laakso , Perttu Koskinen
发明人： Miia Mujunen , Ossi Pastinen , Ilkka Lehtomaki , Simo Laakso , Perttu Koskinen
IPC分类号： C11B3/16 , C10M101/04 , C10L1/188
CPC分类号： C10G53/04 , B01D11/0492 , C02F1/26 , C02F1/66 , C10G1/04 , C10G17/00 , C10G31/09 , C10G53/10 , C10G2400/04 , C10G2400/10 , C10L1/026 , C10L1/08 , C11B1/10
摘要： The invention relates to a method for the recovery of oil from an aqueous solution containing oil and solids, as well as to the use of products obtained by this method. The method of the invention comprises at least the following steps of: Adjusting the oil- and solids-containing aqueous solution to a pH which is acidic, separating an oil- and solids-containing fraction from the acidic aqueous solution with an separation agent which is hydrophilic or assumes a hydrophilic character in response to water, recovering the oil- and solids-containing fraction separated by means of said separation agent, extracting oil away from the solids with a water-insoluble extraction agent, and recovering the oil-containing water-insoluble extraction agent.
摘要翻译：本发明涉及从含有油和固体的水溶液中回收油的方法，以及使用通过该方法得到的产物。本发明的方法至少包括以下步骤：将含油和固体的水溶液调节至酸性的pH，用含有分离剂的分离剂从酸性水溶液中分离含油和固体的级分亲水或具有响应于水的亲水特性，回收通过所述分离剂分离的含有油和固体的馏分，用不溶于水的萃取剂将油从固体中提取出来，并回收含油的水 - 不溶性提取剂。

9. 发明授权

US08870980B2 Process for producing enzymes 有权
标题翻译：生产酶的方法
公开(公告)号：US08870980B2
公开(公告)日：2014-10-28
申请号：US13333188
申请日：2011-12-21
申请人： Annika Malm , Simo Laakso , Ossi Pastinen , Heidi Kahelin , Miia Mujunen
发明人： Annika Malm , Simo Laakso , Ossi Pastinen , Heidi Kahelin , Miia Mujunen
IPC分类号： C10L1/18 , C12N9/00 , C12N9/24 , C12P7/64 , C12N9/42
CPC分类号： C12N9/00 , C12N9/2437 , C12N9/248 , C12P7/6463 , Y02E50/13 , Y02E50/16 , Y02E50/17 , Y02P20/582
摘要： The present invention relates to a process for producing enzymes and single cell oil. The process comprises that microorganisms capable of producing both single cell oil and enzymes are cultivated under conditions suitable for single cell oil production and enzyme production in a single cell oil production process. A microorganism culture comprising single cell oil and enzymes is obtained and at least part of the microorganism culture, of the supernatant and/or microorganism cells separated from the microorganism culture, of protein fraction enriched from the supernatant, and/or of protein fraction obtained from the cells is used as an enzyme preparation or as a source of enzymes. Single cell oil is recovered from the microorganism cells and used as biofuel, component of biofuel or as a starting material for biofuel production. Enzymes produced according to the process are used in the same or in another industrial process.
摘要翻译：本发明涉及一种生产酶和单细胞油的方法。该方法包括在单细胞油生产过程中，能够产生单细胞油和酶的微生物在适于单细胞油生产和酶生产的条件下培养。获得包含单细胞油和酶的微生物培养物，并且从微生物培养物分离的上清液和/或微生物细胞的微生物培养物的至少一部分，富含上清液的蛋白质级分和/或从上清液获得的蛋白质级分细胞被用作酶制剂或酶的来源。单细胞油从微生物细胞中回收并用作生物燃料，生物燃料的组分或作为生物燃料生产的起始材料。根据该方法生产的酶在相同或另一工业过程中使用。

10. 发明申请

US20070128322A1 Method for the treatment of vegetable material, product obtained by the method and use of the product 审中-公开
标题翻译：用于处理植物材料的方法，通过该产品的方法和使用获得的产品
公开(公告)号：US20070128322A1
公开(公告)日：2007-06-07
申请号：US10555387
申请日：2004-05-06
申请人： Jenni Haapiainen , Olavi Myllymaki , Pekka Lehtinen , Ilkka Lehtomaki , Simo Laakso , Markku Patajoki
发明人： Jenni Haapiainen , Olavi Myllymaki , Pekka Lehtinen , Ilkka Lehtomaki , Simo Laakso , Markku Patajoki
IPC分类号： A23P1/00
CPC分类号： C08L5/14 , A23K20/163 , A23L7/115 , A23L33/22 , C08L3/02 , C08L99/00 , C08L2666/26
摘要： The invention relates to a method for improving the solubility of non-starch polysaccharides of a vegetable material. The products prepared by the method and the use of the products are also included in the scope of the invention. The method comprises crushing of the material by mechanical energy to form particles, with at least a major portion of the cells containing non-starch polysaccharides in the material being damaged. Preferably, the non-starch polysaccharides end up in particles as produced by crushing to form particles smaller than that of the initial cell containing these polysaccharides in the material. Disruption of the cells allows effective interaction between the dissolving medium and the polysaccharides to be dissolved, appearing as increased solubility. The material crushing can be performed under the joint effect of pressure, heat and shearing forces. Preferred embodiments include extrusion, expansion and homogenisation under pressure, preferred materials for these treatments comprising oat, rye and fractions of these. The solubility and dissolution circumstances of the product of the invention can be adjusted by mixing the material to be crushed with an ingredient rich in amylopectin.
摘要翻译：本发明涉及一种改善植物材料非淀粉多糖的溶解度的方法。通过该方法制备的产品和产品的使用也包括在本发明的范围内。该方法包括用机械能粉碎材料以形成颗粒，其中至少大部分的细胞含有非淀粉多糖被损坏。优选地，非淀粉多糖最终以粉碎形成的颗粒形成小于在材料中含有这些多糖的初始细胞的颗粒。细胞的破坏允许溶解介质和要溶解的多糖之间的有效相互作用，呈现出增加的溶解度。材料破碎可以在压力，热量和剪切力的联合作用下进行。优选的实施方案包括在压力下的挤出，膨胀和均化，用于这些处理的优选材料包括燕麦，黑麦及其部分。通过将待粉碎的材料与富含支链淀粉的成分混合，可以调节本发明产品的溶解度和溶解情况。

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