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1. 发明申请

US20120159838A1 Process for Producing Enzymes 有权
标题翻译：生产酶的方法
公开(公告)号：US20120159838A1
公开(公告)日：2012-06-28
申请号：US13333188
申请日：2011-12-21
申请人： Annika Malm , Simo Laakso , Ossi Pastinen , Heidi Kahelin , Miia Mujunen
发明人： Annika Malm , Simo Laakso , Ossi Pastinen , Heidi Kahelin , Miia Mujunen
IPC分类号： C10L1/18 , C12N9/14 , C12N9/02 , C10L1/00 , C12N9/90 , C12N9/00 , C12N9/24 , C12N9/88 , C12N9/10
CPC分类号： C12N9/00 , C12N9/2437 , C12N9/248 , C12P7/6463 , Y02E50/13 , Y02E50/16 , Y02E50/17 , Y02P20/582
摘要： The present invention relates to a process for producing enzymes and single cell oil. The process comprises that microorganisms capable of producing both single cell oil and enzymes are cultivated under conditions suitable for single cell oil production and enzyme production in a single cell oil production process. A microorganism culture comprising single cell oil and enzymes is obtained and at least part of the microorganism culture, of the supernatant and/or microorganism cells separated from the microorganism culture, of protein fraction enriched from the supernatant, and/or of protein fraction obtained from the cells is used as an enzyme preparation or as a source of enzymes. Single cell oil is recovered from the microorganism cells and used as biofuel, component of biofuel or as a starting material for biofuel production. Enzymes produced according to the process are used in the same or in another industrial process.
摘要翻译：本发明涉及一种生产酶和单细胞油的方法。该方法包括在单细胞油生产过程中，能够产生单细胞油和酶的微生物在适于单细胞油生产和酶生产的条件下培养。获得包含单细胞油和酶的微生物培养物，并且从微生物培养物分离的上清液和/或微生物细胞的微生物培养物的至少一部分，富含上清液的蛋白质级分和/或从上清液获得的蛋白质级分细胞被用作酶制剂或酶的来源。单细胞油从微生物细胞中回收并用作生物燃料，生物燃料的组分或作为生物燃料生产的起始材料。根据该方法生产的酶在相同或另一工业过程中使用。

2. 发明授权

US08870980B2 Process for producing enzymes 有权
标题翻译：生产酶的方法
公开(公告)号：US08870980B2
公开(公告)日：2014-10-28
申请号：US13333188
申请日：2011-12-21
申请人： Annika Malm , Simo Laakso , Ossi Pastinen , Heidi Kahelin , Miia Mujunen
发明人： Annika Malm , Simo Laakso , Ossi Pastinen , Heidi Kahelin , Miia Mujunen
IPC分类号： C10L1/18 , C12N9/00 , C12N9/24 , C12P7/64 , C12N9/42
CPC分类号： C12N9/00 , C12N9/2437 , C12N9/248 , C12P7/6463 , Y02E50/13 , Y02E50/16 , Y02E50/17 , Y02P20/582
摘要： The present invention relates to a process for producing enzymes and single cell oil. The process comprises that microorganisms capable of producing both single cell oil and enzymes are cultivated under conditions suitable for single cell oil production and enzyme production in a single cell oil production process. A microorganism culture comprising single cell oil and enzymes is obtained and at least part of the microorganism culture, of the supernatant and/or microorganism cells separated from the microorganism culture, of protein fraction enriched from the supernatant, and/or of protein fraction obtained from the cells is used as an enzyme preparation or as a source of enzymes. Single cell oil is recovered from the microorganism cells and used as biofuel, component of biofuel or as a starting material for biofuel production. Enzymes produced according to the process are used in the same or in another industrial process.
摘要翻译：本发明涉及一种生产酶和单细胞油的方法。该方法包括在单细胞油生产过程中，能够产生单细胞油和酶的微生物在适于单细胞油生产和酶生产的条件下培养。获得包含单细胞油和酶的微生物培养物，并且从微生物培养物分离的上清液和/或微生物细胞的微生物培养物的至少一部分，富含上清液的蛋白质级分和/或从上清液获得的蛋白质级分细胞被用作酶制剂或酶的来源。单细胞油从微生物细胞中回收并用作生物燃料，生物燃料的组分或作为生物燃料生产的起始材料。根据该方法生产的酶在相同或另一工业过程中使用。

3. 发明申请

US20110065940A1 Method for the production of fat 有权
标题翻译：生产脂肪的方法
公开(公告)号：US20110065940A1
公开(公告)日：2011-03-17
申请号：US12803350
申请日：2010-06-24
申请人： Heidi Kahelin , Simo Laakso , Ossi Pastinen , Miia Mujunen , Tarja Suomalainen
发明人： Heidi Kahelin , Simo Laakso , Ossi Pastinen , Miia Mujunen , Tarja Suomalainen
IPC分类号： C12P7/64 , C07C53/00 , C12N1/16 , C12N1/18 , C12N1/14
CPC分类号： C12P7/6463 , A23K20/158 , A23L33/115 , C11B1/025 , C11B1/10 , C11C3/003 , Y02E50/13
摘要： The present invention relates to a method for the production of fat with a principal application as transportation biofuel or a component or raw material therefor. According to the method, cell masses, cell suspensions and/or liquid phases formed in the production of single cell oil, and/or biomass-containing side streams or microorganism cell masses for another purpose and/or originating from other sources, are contacted with a fat-production capable microorganism and the organism is allowed to produce fat. The resulting fat is recovered or the microorganism mass is passed to a single-cell oil production process. By means of the invention, the organic matter present in the cell mass and side streams of single-cell oil can be re-utilized for the production of the single-cell oil, thereby improving a total fat yield, as well as reducing an organic load of the side streams.
摘要翻译：本发明涉及作为运输生物燃料或其成分或原料的主要应用的脂肪生产方法。根据该方法，在单细胞油生产中形成的细胞团，细胞悬浮液和/或液相，和/或用于另一目的和/或源自其它来源的含生物质的侧流或微生物细胞块与生产能力的微生物和生物被允许产生脂肪。将所得脂肪回收或将微生物物质转移至单细胞油生产过程。通过本发明，存在于单细胞油的细胞质量和侧流中的有机物质可以再利用于单细胞油的生产，从而提高总脂肪产量，以及还原有机物负载侧流。

4. 发明授权

US08900832B2 Method for the production of fat 有权
标题翻译：生产脂肪的方法
公开(公告)号：US08900832B2
公开(公告)日：2014-12-02
申请号：US12803350
申请日：2010-06-24
申请人： Heidi Kahelin , Simo Laakso , Ossi Pastinen , Miia Mujunen , Tarja Suomalainen
发明人： Heidi Kahelin , Simo Laakso , Ossi Pastinen , Miia Mujunen , Tarja Suomalainen
IPC分类号： C12M1/00
CPC分类号： C12P7/6463 , A23K20/158 , A23L33/115 , C11B1/025 , C11B1/10 , C11C3/003 , Y02E50/13
摘要： The present invention relates to a method for the production of fat with a principal application as transportation biofuel or a component or raw material therefor. According to the method, cell masses, cell suspensions and/or liquid phases formed in the production of single cell oil, and/or biomass-containing side streams or microorganism cell masses for another purpose and/or originating from other sources, are contacted with a fat-production capable microorganism and the organism is allowed to produce fat. The resulting fat is recovered or the microorganism mass is passed to a single-cell oil production process. By means of the invention, the organic matter present in the cell mass and side streams of single-cell oil can be re-utilized for the production of the single-cell oil, thereby improving a total fat yield, as well as reducing an organic load of the side streams.
摘要翻译：本发明涉及作为运输生物燃料或其成分或原料的主要应用的脂肪生产方法。根据该方法，在单细胞油生产中形成的细胞团，细胞悬浮液和/或液相，和/或用于另一目的和/或源自其它来源的含生物质的侧流或微生物细胞块与生产能力的微生物和生物被允许产生脂肪。将所得脂肪回收或将微生物物质转移至单细胞油生产过程。通过本发明，存在于单细胞油的细胞质量和侧流中的有机物质可以再利用于单细胞油的生产，从而提高总脂肪产量，以及还原有机物负载侧流。

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