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1. 发明授权

US06342380B1 Germacrene C synthase gene of Lycopersicon esculentum 有权
标题翻译：番茄的Germacrene C合酶基因
公开(公告)号：US06342380B1
公开(公告)日：2002-01-29
申请号：US09601091
申请日：2000-09-19
申请人： Sheila M. Colby , John E. Crock , Peggy G. Lemaux , Rodney B. Croteau
发明人： Sheila M. Colby , John E. Crock , Peggy G. Lemaux , Rodney B. Croteau
IPC分类号： C12N900
CPC分类号： C12N9/1085 , C12N15/8243 , C12N15/8279 , C12N15/8286 , C12Y402/03009 , Y02A40/162
摘要： Germacrene C synthase genes from Lycopersicon esculentum have been cloned and sequenced. Transgenic expression of germacrene C synthase in plants can result in beneficial and useful characteristics such as increased host resistance to pathogens and herbivores and altered flavor and odor profiles.
摘要翻译：已经克隆并测序了来自番茄的Germacrene C合酶基因。植物中锗菌C合酶的转基因表达可以产生有益和有用的特征，例如增加宿主对病原体和食草动物的抗性以及改变的风味和气味特征。

2. 发明授权

US06451576B1 Sesquiterpene synthases from grand fir (Abies grandis), and methods of use 有权
公开(公告)号：US06451576B1
公开(公告)日：2002-09-17
申请号：US09865171
申请日：2001-05-24
申请人： Rodney Bruce Croteau , Jörg Bohlmann , John E. Crock , Christopher L. Steele
发明人： Rodney Bruce Croteau , Jörg Bohlmann , John E. Crock , Christopher L. Steele
IPC分类号： C12N988
CPC分类号： C12N15/8243 , C12N9/88 , C12N15/8279
摘要： cDNAs encoding, E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase from Grand Fir (Abies grandis) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID No:12; SEQ ID No:19 and SEQ ID No:23) are provided which code for the expression of E-&agr;-bisabolene synthase (SEQ ID No:13), &dgr;-selinene synthase (SEQ ID No:20) and &ggr;-humulene synthase (SEQ ID No:24), respectively, from Grand Fir (Abies grandis). In other aspects, replicable recombinant cloning vehicles are provided which code for E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase, or for a base sequence sufficiently complementary to at least a portion of E-&agr;-bisabolene synthase, &dgr;-selinene synthase or &ggr;-humulene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding E-&agr;-bisabolene synthase, &dgr;-selinene synthase or ,&ggr;-humulene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant sesquiterpene syntheses that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase may be used to obtain expression or enhanced expression of E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase in plants in order to enhance the production of sesquiterpenoids, or may be otherwise employed for the regulation or expression of E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase, or the production of their products.

3. 发明授权

US6008043A Isolation and bacterial expression of a sesquiterpene synthase CDNA clone from peppermint(mentha .chi. piperita, L.) that produces the aphid alarm pheromone (E)-.beta.-farnesene 有权
标题翻译：来自薄荷（薄荷胡椒，L.）的倍半萜合酶cDNA克隆的分离和细菌表达，产生蚜虫报警信息素（E） - 法呢烯
公开(公告)号：US6008043A
公开(公告)日：1999-12-28
申请号：US166460
申请日：1998-10-05
申请人： Rodney Bruce Croteau , Mark Raymond Wildung , John E. Crock
发明人： Rodney Bruce Croteau , Mark Raymond Wildung , John E. Crock
IPC分类号： C12N9/88 , C12P5/02 , C12N5/00 , C12N15/52 , C12N15/63
CPC分类号： C12P5/007 , C12N9/88
摘要： A cDNA encoding (E)-.beta.-farnesene synthase from peppermint (Mentha piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of (E)-.beta.-farnesene synthase (SEQ ID NO:2), from peppermint (Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for (E)-.beta.-farnesene synthase, or for a base sequence sufficiently complementary to at least a portion of (E)-.beta.-farnesene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (E)-.beta.-farnesene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant (E)-.beta.-farnesene synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant (E)-.beta.-farnesene synthase may be used to obtain expression or enhanced expression of (E)-.beta.-farnesene synthase in plants in order to enhance the production of (E)-.beta.-farnesene, or may be otherwise employed for the regulation or expression of (E)-.beta.-farnesene synthase, or the production of its product.
摘要翻译：已经分离并测序了从薄荷（Mentha piperita）编码（E）-β-法呢烯合成酶的cDNA，并测定了相应的氨基酸序列。因此，提供了从薄荷（薄荷（胡椒））编码（E）-β-法呢烯合酶（SEQ ID NO：2）的表达的分离的DNA序列（SEQ ID NO：1）。在其它方面，提供编码（E）-β-法呢烯合酶或与至少一部分（E）-β-法呢烯合酶DNA或RNA充分互补的碱基序列的可重复的重组克隆载体，以使其能够与其进行杂交。在其它方面，提供已经用编码（E）-β-法呢烯合成酶的重组克隆载体和/或DNA序列转化，转染，感染和/或注射的修饰的宿主细胞。因此，提供了用于重组表达上述重组（E）-β-法呢烯合成酶的系统和方法，其可用于促进其显着量的产生，分离和纯化。可以使用重组（E）-β-法呢烯合酶来获得（E）-β-法呢烯合酶在植物中的表达或增强的表达，以增强（E）-β-法呢烯的生产，或者可以另外用于（E）-β-法呢烯合成酶的调节或表达，或其产物的生产。

4. 发明授权

US06265639B1 Gymnosperm nucleic acid molecules encoding sesquiterpene synthases and methods of use 有权
标题翻译：编码倍半萜合酶的裸子核酸分子及其使用方法
公开(公告)号：US06265639B1
公开(公告)日：2001-07-24
申请号：US09234393
申请日：1999-01-20
申请人： Rodney Bruce Croteau , Jörg Bohlmann , Reinhard Jetter , John E. Crock , Christopher L. Steele
发明人： Rodney Bruce Croteau , Jörg Bohlmann , Reinhard Jetter , John E. Crock , Christopher L. Steele
IPC分类号： A01H500
CPC分类号： C12N15/8243 , C12N9/88 , C12N15/8279
摘要： cDNAs encoding E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase from Grand Fir (Abies grandis) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID No:12 and SEQ ID No:19 and SEQ ID No:23) are provided which code for the expression of E-&agr;-bisabolene synthase (SEQ ID No:13), &dgr;-selinene synthase (SEQ ID No:20) and &ggr;-humulene synthase (SEQ ID No: 24), respectively, from Grand Fir (Abies grandis). In other aspects, replicable recombinant cloning vehicles are provided which code for E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase, or for a base sequence sufficiently complementary to at least a portion of E-&agr;-bisabolene synthase, &dgr;-selinene synthase or &ggr;-humulene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding E-&agr;-bisabolene synthase, &dgr;-selinene synthase or &ggr;-humulene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant sesquiterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase may be used to obtain expression or enhanced expression of E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase in plants, or may be otherwise employed for the regulation or expression of E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase.
摘要翻译：已经分离并测序了来自Grand Fir（Abies grandis）的编码E-α-双酯合成酶，δ-塞来林合成酶和γ-胡萝卜烯合酶的cDNA，并测定了相应的氨基酸序列。因此，提供了编码E-α-双酯合成酶（SEQ ID No：13），δ-赛里烯合成酶（SEQ ID No：13）的表达的分离的DNA序列（SEQ ID No：12和SEQ ID No：19和SEQ ID No：23）（SEQ ID No：20）和来自Grand Fir（Abies grandis））的γ-胡红烯合酶（SEQ ID No：24）。在其它方面，提供了可复制的重组克隆载体，其编码E-α-二磷酸合成酶，δ-塞来林合成酶和γ-胡红烯合酶，或用于与至少一部分E-α-二磷酸合酶充分互补的碱基序列， δ-赛琳娜合成酶或γ-胡红烯合酶DNA或RNA以进行杂交。在其它方面，提供已经用编码E-α-二磷酸合成酶，δ-塞来林合成酶或γ-葎草酮合酶的重组克隆载体和/或DNA序列进行转化，转染，感染和/或注射的修饰的宿主细胞。因此，提供了用于重组表达上述重组倍半萜烯合酶的系统和方法，其可用于促进其显着量的产生，分离和纯化。可以使用重组的E-α-二磷酸合成酶，δ-赛里烯合成酶和γ-胡梅烯合酶来获得植物中E-α-二磷酸合成酶，δ-塞来林合成酶和γ-胡萝卜烯合酶的表达或增强的表达，或者可以以其它方式用于调节或表达E-α-双酯合成酶，δ-塞来那烯合酶和γ-葎草酮合酶。

5. 发明授权

US06846659B2 Isolation and bacterial expression of a sesquiterpene synthase cDNA clone from peppermint (Mentha x piperita, L.) that produces the aphid alarm pheromone (E)-β-farnesene 失效
标题翻译：从薄荷（Mentha x piperita，L.）中产生蚜虫报警信息素（E） - 法呢烯的倍半萜合酶cDNA克隆的分离和细菌表达
公开(公告)号：US06846659B2
公开(公告)日：2005-01-25
申请号：US09860282
申请日：2001-05-17
申请人： Rodney Bruce Croteau , John E. Crock
发明人： Rodney Bruce Croteau , John E. Crock
IPC分类号： C12N9/88 , C12P5/02 , C12N9/00 , C12N5/14
CPC分类号： C12P5/007 , C12N9/88
摘要： A cDNA encoding (E)-β-farnesene synthase from peppermint (Mentha piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of (E)-β-farnesene synthase (SEQ ID NO:2), from peppermint (Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for (E)-β-farnesene synthase, or for a base sequence sufficiently complementary to at least a portion of (E)-β-farnesene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (E)-β-farnesene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant (E)-β-famesene synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant (E)-β-farnesene synthase may be used to obtain expression or enhanced expression of (E)-β-famesene synthase in plants in order to enhance the production of (E)-β-farnesene, or may be otherwise employed for the regulation or expression of (E)-β-farnesene synthase, or the production of its product.
摘要翻译：已经分离了来自薄荷（薄荷胡椒）的编码（E） - 法呢烯合成酶的cDNA并测序，并确定了相应的氨基酸序列。因此，提供了从薄荷（Mentha piperita）编码（E） - 法呢基合酶（SEQ ID NO：2）的表达的分离的DNA序列（SEQ ID NO：1）。在其它方面，提供了编码（E） - 法呢烯合成酶的可重复的重组克隆载体，或与（E） - 法呢烯合成酶DNA或RNA的至少一部分充分互补的碱基序列，以使其能够与其进行杂交。在另一方面，提供已经用编码（E） - 法呢烯合成酶的重组克隆载体和/或DNA序列转化，转染，感染和/或注射的修饰的宿主细胞。因此，提供了用于重组表达上述重组（E）-β-甲酰基合成酶的系统和方法，其可用于促进其显着量的产生，分离和纯化。可以使用重组（E） - 法呢烯合成酶来获得植物中（E）-β-fameene合酶的表达或增强的表达，以增强（E） - 法呢烯的生产，或者可以另外用于（E） - 法呢烯合成酶的调节或表达，或其产物的生产。

6. 发明授权

US06258602B1 Isolation and bacterial expression of a sesquiterpene synthase cDNA clone from peppermint (mentha x piperita, L.) that produces the aphid alarm pheromone (E)-&bgr;-farnesene 有权
标题翻译：来自薄荷（薄荷胡椒，L.）的倍半萜合酶cDNA克隆的分离和细菌表达，产生蚜虫报警信息素（E） - 法呢烯
公开(公告)号：US06258602B1
公开(公告)日：2001-07-10
申请号：US09361718
申请日：1999-07-27
申请人： Rodney Bruce Croteau , Mark Raymond Wildung , John E. Crock
发明人： Rodney Bruce Croteau , Mark Raymond Wildung , John E. Crock
IPC分类号： C12N514
CPC分类号： C12P5/007 , C12N9/88
摘要： A cDNA encoding (E)-&bgr;-farnesene synthase from peppermint (Mentha piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO: 1) is provided which codes for the expression of (E)-&bgr;-farnesene synthase (SEQ ID NO:2), from peppermint (Mentha piperita). In other aspects replicable recombinant cloning vehicles are provided which code for (E)-&bgr;-farnesene synthase, or for a base sequence sufficiently complementary to at least a portion of (E)-&bgr;-farnesene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (E)-&bgr;-farnesene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant (E)-&bgr;-farnesene synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant (E)-&bgr;-farnesene synthase may be used to obtain expression or enhanced expression of (E)-&bgr;-farnesene synthase in plants in order to enhance the production of (E)-&bgr;-farnesene, or may be otherwise employed for the regulation or expression of (E)-&bgr;-farnesene synthase, or the production of its product.
摘要翻译：已经分离了来自薄荷（薄荷胡椒）的编码（E） - 法呢烯合成酶的cDNA并测序，并确定了相应的氨基酸序列。因此，提供了从薄荷（Mentha piperita）编码（E） - 法呢基合酶（SEQ ID NO：2）的表达的分离的DNA序列（SEQ ID NO：1）。在其它方面，提供可复制的重组克隆载体，其编码（E） - 法呢烯合成酶，或与（E） - 法呢烯合成酶DNA或RNA的至少一部分充分互补的碱基序列，以使其能够进行杂交。在另一方面，提供已经用编码（E） - 法呢烯合成酶的重组克隆载体和/或DNA序列转化，转染，感染和/或注射的修饰的宿主细胞。因此，提供了用于重组表达上述重组（E） - 法呢烯合成酶的系统和方法，其可用于促进其显着量的产生，分离和纯化。可以使用重组（E） - 法呢烯合酶来获得植物中（E） - 法呢烯合成酶的表达或增强的表达，以增强（E） - 法呢烯的生产，或者可以另外用于（E） - 法呢烯合成酶的调节或表达，或其产物的生产。

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