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1. 发明授权

US06265639B1 Gymnosperm nucleic acid molecules encoding sesquiterpene synthases and methods of use 有权
标题翻译：编码倍半萜合酶的裸子核酸分子及其使用方法
公开(公告)号：US06265639B1
公开(公告)日：2001-07-24
申请号：US09234393
申请日：1999-01-20
申请人： Rodney Bruce Croteau , Jörg Bohlmann , Reinhard Jetter , John E. Crock , Christopher L. Steele
发明人： Rodney Bruce Croteau , Jörg Bohlmann , Reinhard Jetter , John E. Crock , Christopher L. Steele
IPC分类号： A01H500
CPC分类号： C12N15/8243 , C12N9/88 , C12N15/8279
摘要： cDNAs encoding E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase from Grand Fir (Abies grandis) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID No:12 and SEQ ID No:19 and SEQ ID No:23) are provided which code for the expression of E-&agr;-bisabolene synthase (SEQ ID No:13), &dgr;-selinene synthase (SEQ ID No:20) and &ggr;-humulene synthase (SEQ ID No: 24), respectively, from Grand Fir (Abies grandis). In other aspects, replicable recombinant cloning vehicles are provided which code for E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase, or for a base sequence sufficiently complementary to at least a portion of E-&agr;-bisabolene synthase, &dgr;-selinene synthase or &ggr;-humulene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding E-&agr;-bisabolene synthase, &dgr;-selinene synthase or &ggr;-humulene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant sesquiterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase may be used to obtain expression or enhanced expression of E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase in plants, or may be otherwise employed for the regulation or expression of E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase.
摘要翻译：已经分离并测序了来自Grand Fir（Abies grandis）的编码E-α-双酯合成酶，δ-塞来林合成酶和γ-胡萝卜烯合酶的cDNA，并测定了相应的氨基酸序列。因此，提供了编码E-α-双酯合成酶（SEQ ID No：13），δ-赛里烯合成酶（SEQ ID No：13）的表达的分离的DNA序列（SEQ ID No：12和SEQ ID No：19和SEQ ID No：23）（SEQ ID No：20）和来自Grand Fir（Abies grandis））的γ-胡红烯合酶（SEQ ID No：24）。在其它方面，提供了可复制的重组克隆载体，其编码E-α-二磷酸合成酶，δ-塞来林合成酶和γ-胡红烯合酶，或用于与至少一部分E-α-二磷酸合酶充分互补的碱基序列， δ-赛琳娜合成酶或γ-胡红烯合酶DNA或RNA以进行杂交。在其它方面，提供已经用编码E-α-二磷酸合成酶，δ-塞来林合成酶或γ-葎草酮合酶的重组克隆载体和/或DNA序列进行转化，转染，感染和/或注射的修饰的宿主细胞。因此，提供了用于重组表达上述重组倍半萜烯合酶的系统和方法，其可用于促进其显着量的产生，分离和纯化。可以使用重组的E-α-二磷酸合成酶，δ-赛里烯合成酶和γ-胡梅烯合酶来获得植物中E-α-二磷酸合成酶，δ-塞来林合成酶和γ-胡萝卜烯合酶的表达或增强的表达，或者可以以其它方式用于调节或表达E-α-双酯合成酶，δ-塞来那烯合酶和γ-葎草酮合酶。

2. 发明授权

US06451576B1 Sesquiterpene synthases from grand fir (Abies grandis), and methods of use 有权
公开(公告)号：US06451576B1
公开(公告)日：2002-09-17
申请号：US09865171
申请日：2001-05-24
申请人： Rodney Bruce Croteau , Jörg Bohlmann , John E. Crock , Christopher L. Steele
发明人： Rodney Bruce Croteau , Jörg Bohlmann , John E. Crock , Christopher L. Steele
IPC分类号： C12N988
CPC分类号： C12N15/8243 , C12N9/88 , C12N15/8279
摘要： cDNAs encoding, E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase from Grand Fir (Abies grandis) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID No:12; SEQ ID No:19 and SEQ ID No:23) are provided which code for the expression of E-&agr;-bisabolene synthase (SEQ ID No:13), &dgr;-selinene synthase (SEQ ID No:20) and &ggr;-humulene synthase (SEQ ID No:24), respectively, from Grand Fir (Abies grandis). In other aspects, replicable recombinant cloning vehicles are provided which code for E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase, or for a base sequence sufficiently complementary to at least a portion of E-&agr;-bisabolene synthase, &dgr;-selinene synthase or &ggr;-humulene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding E-&agr;-bisabolene synthase, &dgr;-selinene synthase or ,&ggr;-humulene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant sesquiterpene syntheses that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase may be used to obtain expression or enhanced expression of E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase in plants in order to enhance the production of sesquiterpenoids, or may be otherwise employed for the regulation or expression of E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase, or the production of their products.

3. 发明授权

US07273755B2 Compositions and methods for altering biosynthesis of taxanes and taxane-related compounds 失效
标题翻译：用于改变紫杉烷类和紫杉烷类化合物生物合成的组合物和方法
公开(公告)号：US07273755B2
公开(公告)日：2007-09-25
申请号：US10361290
申请日：2003-02-10
申请人： Christopher L. Steele , Yijun Chen , Brian A. Dougherty , Sandra Hofstead , Kin S. Lam , Wenying Li , Zizhuo Xing
发明人： Christopher L. Steele , Yijun Chen , Brian A. Dougherty , Sandra Hofstead , Kin S. Lam , Wenying Li , Zizhuo Xing
IPC分类号： C12N5/10 , C12P7/52 , C12N9/10 , C12N9/90 , C12N5/04 , C07H21/04
CPC分类号： C12N5/0025 , C12N9/90 , C12P17/02
摘要： Isolated nucleic acid and amino acid sequences for phenylalanine aminomutase enzyme and methods for purifying this enzyme are provided. Methods for altering biosynthesis of compounds in plant cell cultures are also provided. In particular, methods for altering production of taxanes and taxane-related compounds are provided.
摘要翻译：提供了用于苯丙氨酸氨基变位酶的分离的核酸和氨基酸序列以及用于纯化该酶的方法。还提供了用于改变植物细胞培养物中化合物的生物合成的方法。特别地，提供了用于改变紫杉烷类和紫杉烷类化合物生产的方法。

4. 发明授权

US07038113B1 Genetic manipulation of isoflavonoids 失效
标题翻译：异黄酮的遗传操作
公开(公告)号：US07038113B1
公开(公告)日：2006-05-02
申请号：US09936190
申请日：2000-03-08
申请人： Richard A. Dixon , Christopher L. Steele
发明人： Richard A. Dixon , Christopher L. Steele
IPC分类号： C12N15/29 , C12N15/63 , C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N9/0077 , C12N15/8243 , C12N15/825 , C12N15/8261 , Y02A40/146
摘要： Soybean and Medicago truncatula CYP93C genes have been isolated which encode a cytochrome P450 that can catalyze the aryl migration of a flavanone to yield an isoflavanone intermediate or an isoflavone. Plants can now be genetically engineered to produce isoflavones that provide potential human health benefits and increase disease resistance in plants. Isoflavones can now be produced in transgenic plants species in which isoflavones do not naturally occur, i.e., in species other than legumes. Alternatively, introducing infection-inducible isoflavonoid biosynthesis into non-legumes qualitatively complements these plants phytoalexin defenses against microbial pathogens, whereas over-expression of the isoflavonoid pathway in legumes quantitatively increases this defense response. Finally, modifying the extend of production of isoflavonoids in legume roots positively impacts nodulation efficiency and therefore plant yield.
摘要翻译：已经分离出大豆和苜蓿Truncatula CYP93C基因，其编码细胞色素P450，其可以催化黄烷酮的芳基迁移以产生异黄酮中间体或异黄酮。植物现在可以进行基因工程，以生产提供潜在的人类健康益处并增加植物抗病性的异黄酮。异黄酮现在可以在其中不是天然存在异黄酮的转基因植物物种中生产，即在除豆类之外的物种中。或者，将感染诱导型异黄酮生物合成引入非豆科植物定性补充这些植物对微生物病原体的植物抗毒素抗性，而豆类中异黄酮途径的过表达量则定量增加了这种防御反应。最后，修改豆科植物中异黄酮生产的延伸对结瘤效率和植株产量产生积极影响。

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