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1. 发明授权

US6043072A Nucleic acids encoding Taxus geranylgeranyl diphosphate synthase, and methods of use 有权
标题翻译：编码红豆杉香叶基二磷酸合成酶的核酸及其使用方法
公开(公告)号：US6043072A
公开(公告)日：2000-03-28
申请号：US187050
申请日：1998-11-05
申请人： Rodney B. Croteau , Jerry L. Hefner
发明人： Rodney B. Croteau , Jerry L. Hefner
IPC分类号： C12N15/09 , C12N5/10 , C12N9/10 , C12N15/82 , C12R1/91 , C07H21/04 , C07K1/00 , C12N1/20 , C12N15/00
CPC分类号： C12N9/1085 , C12N15/8243 , C12Y205/01029
摘要： A cDNA encoding geranylgeranyl diphosphate synthase from Canadian Yew (Taxus canadensis) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of geranylgeranyl diphosphate synthase (SEQ ID NO:2), from Canadian Yew (Taxus canadensis). In other aspects, replicable recombinant cloning vehicles are provided which code for geranylgeranyl diphosphate synthase, or for a base sequence sufficiently complementary to at least a portion of geranylgeranyl diphosphate synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding geranylgeranyl diphosphate synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant geranylgeranyl diphosphate synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant geranylgeranyl diphosphate synthase may be used to obtain expression or enhanced expression of geranylgeranyl diphosphate synthase in plants in order to enhance the production of geranylgeranyl diphosphate, or diterpenes derived therefrom, or may be otherwise employed for the regulation or expression of geranylgeranyl diphosphate synthase, or the production of its products.
摘要翻译：已经分离并测序了来自加拿大红豆杉（Taxus canadensis）的编码香叶基香叶基二磷酸合成酶的cDNA，并测定了相应的氨基酸序列。因此，提供编码来自加拿大红豆杉（Taxus canadensis）的香叶基香叶基二磷酸合成酶（SEQ ID NO：2）的表达的分离的DNA序列（SEQ ID NO：1）。在其它方面，提供编码香叶基香叶基二磷酸合酶的可重复克隆载体，或与至少一部分香叶基香叶基二磷酸合成酶DNA或RNA足够互补的碱基序列，以使其能够进行杂交。在另一方面，提供已经用编码香叶基香叶基二磷酸合成酶的重组克隆载体和/或DNA序列转化，转染，感染和/或注射的修饰的宿主细胞。因此，提供了用于重组表达上述重组er牛儿基ger牛儿基二磷酸合成酶的系统和方法，其可用于促进其显着量的产生，分离和纯化。可以使用重组er牛儿基ger牛儿基二磷酸合成酶在植物中获得表达或增强er牛儿基ger牛儿基二磷酸合成酶的表达，以增强er牛儿基ger牛儿基二磷酸酯的产生，或由此衍生的二萜烯的生产，或可用于调节或表达香叶基香叶基二磷酸合成酶，或其产品的生产。

2. 发明授权

US07005283B2 Cytochrome P450 oxygenases and their uses 失效
标题翻译：细胞色素P450加氧酶及其用途
公开(公告)号：US07005283B2
公开(公告)日：2006-02-28
申请号：US10884115
申请日：2004-07-01
申请人： Rodney B. Croteau , Anne Schoendorf , Stefan Jennewein
发明人： Rodney B. Croteau , Anne Schoendorf , Stefan Jennewein
IPC分类号： C12N9/02 , C12N1/20 , C12N15/00 , C12P21/014 , C07H21/04
CPC分类号： C12N9/0077 , A01K2217/05 , C12P17/02
摘要： Oxygenase enzymes and the use of such enzymes to produce paclitaxel (Taxol™), related taxoids, as well as intermediates in the Taxol biosynthetic pathway are disclosed. Also disclosed are nucleic acid sequences encoding the oxygenase enzymes.
摘要翻译：公开了加氧酶和这些酶在紫杉醇生物合成途径中产生紫杉醇（Taxol TM），相关紫杉类以及中间体的用途。还公开了编码加氧酶的核酸序列。

3. 发明授权

US06287835B1 Transacylases of the paclitaxel biosynthetic pathway 有权
标题翻译：紫杉醇生物合成途径的转酰胺酶
公开(公告)号：US06287835B1
公开(公告)日：2001-09-11
申请号：US09457046
申请日：1999-12-07
申请人： Rodney B. Croteau , Kevin D. Walker , Anne Schoendorf , Mark R. Wildung
发明人： Rodney B. Croteau , Kevin D. Walker , Anne Schoendorf , Mark R. Wildung
IPC分类号： C12N910
CPC分类号： C12P17/02 , C12N9/1029
摘要： Transacylase enzymes and the use of such enzymes to produce Taxol™, related taxoids, as well as intermediates in the Taxol™ biosynthetic pathway are disclosed. Also disclosed are nucleic acid sequences encoding the transacylase enzymes.
摘要翻译：公开了转酰胺酶和这些酶在Taxol TM生物合成途径中产生Taxol TM，相关紫杉类以及中间体的用途。还公开了编码转酰酶的核酸序列。

4. 发明授权

US6114160A Compositions and methods for taxol biosynthesis 有权
标题翻译：紫杉醇生物合成的组合物和方法
公开(公告)号：US6114160A
公开(公告)日：2000-09-05
申请号：US315861
申请日：1999-05-20
申请人： Rodney B. Croteau , Mark R. Wildung
发明人： Rodney B. Croteau , Mark R. Wildung
IPC分类号： C12N15/09 , A01H5/00 , C07K7/00 , C07K14/415 , C07K16/16 , C12N5/10 , C12N9/00 , C12N9/88 , C12N9/90 , C12N15/82 , C12P21/02 , C12Q1/68 , C07H21/04 , C12N1/20 , C12N15/00
CPC分类号： C12N9/88 , C12N15/8243 , C12N9/90 , C07K2319/00
摘要： The taxadiene synthase gene of Pacific yew has been cloned and its nucleic acid and polypeptide sequence is presented. Truncation or removal of the transit peptide increases expression of the cloned taxadiene synthase gene expression in E. coli cells.
摘要翻译：已经克隆了太平洋红豆杉的紫杉二烯合酶基因，并提出了其核酸和多肽序列。转运肽的截断或去除增加了在大肠杆菌细胞中克隆的二聚体合成酶基因表达的表达。

5. 发明授权

US08426684B2 Isolated menthone reductase and nucleic acid molecules encoding same 失效
标题翻译：孤立的薄荷酮还原酶和编码相同的核酸分子
公开(公告)号：US08426684B2
公开(公告)日：2013-04-23
申请号：US11569493
申请日：2004-06-15
申请人： Rodney B. Croteau , Edward M. Davis , Kerry L. Ringer
发明人： Rodney B. Croteau , Edward M. Davis , Kerry L. Ringer
IPC分类号： C12N15/29 , C12N15/52 , C12N15/82
CPC分类号： C12N9/0006 , C12N15/8243
摘要： The present invention provides isolated menthone reductase proteins, isolated nucleic acid molecules encoding menthone reductase proteins, methods for expressing and isolating menthone reductase proteins, and transgenic plants expressing elevated levels of menthone reductase protein.
摘要翻译：本发明提供了分离的薄荷酮还原酶蛋白，编码薄荷酮还原酶蛋白的分离的核酸分子，表达和分离薄荷酮还原酶蛋白的方法，以及表达高水平的薄荷酮还原酶蛋白质的转基因植物。

6. 发明授权

US07402417B2 P450 oxygenases and methods of use 有权
标题翻译： P450加氧酶及使用方法
公开(公告)号：US07402417B2
公开(公告)日：2008-07-22
申请号：US10565233
申请日：2004-07-21
申请人： Rodney B. Croteau , Robert Long , Stefan Jennewein
发明人： Rodney B. Croteau , Robert Long , Stefan Jennewein
IPC分类号： C12N9/02 , C12N1/20 , C12N15/00 , C12P7/66 , C12P21/04 , C12Q1/26 , C07H21/04 , C12Q1/68 , C12Q1/00
CPC分类号： C12N9/0069
摘要： This disclosure provides the nucleic acid and protein sequences of novel P450 oxygenases that utilize, at least, taxoid substrates, including taxadiene isomers. The disclosed oxygenases, such as a taxoid 5α-hydroxylase, hydroxylate, at least, the C5 position of a taxoid. Also provided are methods of introducing oxygen at the C5 position of a taxoid and methods of producing the anti-cancer drug, paclitaxel (also known as Taxol™), and other taxoids, such as paclitaxel intermediates.
摘要翻译：本公开提供使用至少紫杉烷底物（包括紫杉二烯异构体）的新型P450加氧酶的核酸和蛋白质序列。公开的加氧酶，例如紫杉烷类5α-羟化酶，羟基化物，至少是紫杉烷的C5位置。还提供了在紫杉烷的C5位置引入氧的方法和制备抗癌药物紫杉醇（也称为Taxol TM）以及其他类紫杉醇如紫杉醇中间体的方法。

7. 发明授权

US06610527B1 Compositions and methods for Taxol biosynthesis 失效
标题翻译：紫杉醇生物合成的组成和方法
公开(公告)号：US06610527B1
公开(公告)日：2003-08-26
申请号：US09593253
申请日：2000-06-13
申请人： Rodney B. Croteau , Mark R. Wildung
发明人： Rodney B. Croteau , Mark R. Wildung
IPC分类号： C12N988
CPC分类号： C12N9/88 , C07K2319/00 , C12N9/90 , C12N15/8243
摘要： The taxadiene synthase gene of Pacific yew has been cloned and its nucleic acid and polypeptide sequence is presented. Truncation or removal of the transit peptide increases expression of the cloned taxadiene synthase gene expression in E. coli cells.
摘要翻译：已经克隆了太平洋红豆杉的紫杉二烯合酶基因，并提出了其核酸和多肽序列。转运肽的截断或去除增加了在大肠杆菌细胞中克隆的二聚体合成酶基因表达的表达。

8. 发明授权

US06303330B1 Geranyl diphosphate synthase large subunit, and methods of use 有权
标题翻译：香叶基二磷酸合成酶大亚基，以及使用方法
公开(公告)号：US06303330B1
公开(公告)日：2001-10-16
申请号：US09420211
申请日：1999-10-18
申请人： Rodney B. Croteau , Charles C. Burke , Mark R. Wildung
发明人： Rodney B. Croteau , Charles C. Burke , Mark R. Wildung
IPC分类号： C12N1563
CPC分类号： C12N9/1085 , A61K48/00 , C12N15/8243
摘要： A cDNA encoding geranyl diphosphate synthase large subunit from peppermint has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Replicable recombinant cloning vehicles are provided which code for geranyl diphosphate synthase large subunit). In another aspect, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding geranyl diphosphate synthase large subunit. In yet another aspect, the present invention provides isolated, recombinant geranyl diphosphate synthase protein comprising an isolated, recombinant geranyl diphosphate synthase large subunit protein and an isolated, recombinant geranyl diphosphate synthase small subunit protein. Thus, systems and methods are provided for the recombinant expression of geranyl diphosphate synthase.
摘要翻译：已经分离并测序了从薄荷中编码香叶基二磷酸合酶大亚基的cDNA，并测定了相应的氨基酸序列。提供可复制的重组克隆载体，其编码香叶基二磷酸合酶大亚基）。在另一方面，提供已经用编码香叶基二磷酸合成酶大亚基的重组克隆载体和/或DNA序列转化，转染，感染和/或注射的修饰的宿主细胞。在另一方面，本发明提供了分离的重组香叶基二磷酸合酶蛋白，其包含分离的重组香叶基二磷酸合酶大亚基蛋白和分离的重组香叶基二磷酸合酶小亚基蛋白。因此，提供了重组表达香叶基二磷酸合成酶的系统和方法。

9. 发明申请

US20090300801A1 ISOLATED MENTHONE REDUCTASE AND NUCLEIC ACID MOLECULES ENCODING SAME 失效
标题翻译：分离的减水剂和核酸分子编码它们
公开(公告)号：US20090300801A1
公开(公告)日：2009-12-03
申请号：US11569493
申请日：2004-06-15
申请人： Rodney B. Croteau , Edward M. Davis , Kerry L. Ringer
发明人： Rodney B. Croteau , Edward M. Davis , Kerry L. Ringer
IPC分类号： A01H5/00 , C07H21/00 , C12N15/74 , C12N5/04 , C12N5/10 , C12N5/00 , C12N9/02 , C12P21/02
CPC分类号： C12N9/0006 , C12N15/8243
摘要： The present invention provides isolated menthone reductase proteins, isolated nucleic acid molecules encoding menthone reductase proteins, methods for expressing and isolating menthone reductase proteins, and transgenic plants expressing elevated levels of menthone reductase protein.
摘要翻译：本发明提供了分离的薄荷酮还原酶蛋白，编码薄荷酮还原酶蛋白的分离的核酸分子，表达和分离薄荷酮还原酶蛋白的方法，以及表达高水平的薄荷酮还原酶蛋白质的转基因植物。

10. 发明授权

US07416872B2 Taxoid oxygenases and their uses 失效
标题翻译：紫杉烷类加氧酶及其用途
公开(公告)号：US07416872B2
公开(公告)日：2008-08-26
申请号：US11242662
申请日：2005-10-03
申请人： Rodney B. Croteau , Anne Schoendorf , Stefan Jennewein
发明人： Rodney B. Croteau , Anne Schoendorf , Stefan Jennewein
IPC分类号： C12N9/02 , C12N15/00 , C12N1/20 , C12N5/00 , C12P21/04 , C12Q1/26 , C07H21/04 , C12Q1/00 , C12Q1/68
CPC分类号： C12N9/0077 , A01K2217/05 , C12P17/02
摘要： Oxygenase enzymes and the use of such enzymes to produce paclitaxel (Taxol™), related taxoids, as well as intermediates in the Taxol biosynthetic pathway are disclosed. Also disclosed are nucleic acid sequences encoding the oxygenase enzymes.
摘要翻译：公开了加氧酶和这些酶在紫杉醇生物合成途径中产生紫杉醇（Taxol TM），相关紫杉类以及中间体的用途。还公开了编码加氧酶的核酸序列。

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