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    • 1. 发明授权
    • Nucleic acids encoding Taxus geranylgeranyl diphosphate synthase, and
methods of use
    • 编码红豆杉香叶基二磷酸合成酶的核酸及其使用方法
    • US6043072A
    • 2000-03-28
    • US187050
    • 1998-11-05
    • Rodney B. CroteauJerry L. Hefner
    • Rodney B. CroteauJerry L. Hefner
    • C12N15/09C12N5/10C12N9/10C12N15/82C12R1/91C07H21/04C07K1/00C12N1/20C12N15/00
    • C12N9/1085C12N15/8243C12Y205/01029
    • A cDNA encoding geranylgeranyl diphosphate synthase from Canadian Yew (Taxus canadensis) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of geranylgeranyl diphosphate synthase (SEQ ID NO:2), from Canadian Yew (Taxus canadensis). In other aspects, replicable recombinant cloning vehicles are provided which code for geranylgeranyl diphosphate synthase, or for a base sequence sufficiently complementary to at least a portion of geranylgeranyl diphosphate synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding geranylgeranyl diphosphate synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant geranylgeranyl diphosphate synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant geranylgeranyl diphosphate synthase may be used to obtain expression or enhanced expression of geranylgeranyl diphosphate synthase in plants in order to enhance the production of geranylgeranyl diphosphate, or diterpenes derived therefrom, or may be otherwise employed for the regulation or expression of geranylgeranyl diphosphate synthase, or the production of its products.
    • 已经分离并测序了来自加拿大红豆杉(Taxus canadensis)的编码香叶基香叶基二磷酸合成酶的cDNA,并测定了相应的氨基酸序列。 因此,提供编码来自加拿大红豆杉(Taxus canadensis)的香叶基香叶基二磷酸合成酶(SEQ ID NO:2)的表达的分离的DNA序列(SEQ ID NO:1)。 在其它方面,提供编码香叶基香叶基二磷酸合酶的可重复克隆载体,或与至少一部分香叶基香叶基二磷酸合成酶DNA或RNA足够互补的碱基序列,以使其能够进行杂交。 在另一方面,提供已经用编码香叶基香叶基二磷酸合成酶的重组克隆载体和/或DNA序列转化,转染,感染和/或注射的修饰的宿主细胞。 因此,提供了用于重组表达上述重组er牛儿基ger牛儿基二磷酸合成酶的系统和方法,其可用于促进其显着量的产生,分离和纯化。 可以使用重组er牛儿基ger牛儿基二磷酸合成酶在植物中获得表达或增强er牛儿基ger牛儿基二磷酸合成酶的表达,以增强er牛儿基ger牛儿基二磷酸酯的产生,或由此衍生的二萜烯的生产,或可用于调节或表达香叶基香叶基二磷酸合成酶,或 其产品的生产。
    • 8. 发明授权
    • Geranyl diphosphate synthase large subunit, and methods of use
    • 香叶基二磷酸合成酶大亚基,以及使用方法
    • US06303330B1
    • 2001-10-16
    • US09420211
    • 1999-10-18
    • Rodney B. CroteauCharles C. BurkeMark R. Wildung
    • Rodney B. CroteauCharles C. BurkeMark R. Wildung
    • C12N1563
    • C12N9/1085A61K48/00C12N15/8243
    • A cDNA encoding geranyl diphosphate synthase large subunit from peppermint has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Replicable recombinant cloning vehicles are provided which code for geranyl diphosphate synthase large subunit). In another aspect, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding geranyl diphosphate synthase large subunit. In yet another aspect, the present invention provides isolated, recombinant geranyl diphosphate synthase protein comprising an isolated, recombinant geranyl diphosphate synthase large subunit protein and an isolated, recombinant geranyl diphosphate synthase small subunit protein. Thus, systems and methods are provided for the recombinant expression of geranyl diphosphate synthase.
    • 已经分离并测序了从薄荷中编码香叶基二磷酸合酶大亚基的cDNA,并测定了相应的氨基酸序列。 提供可复制的重组克隆载体,其编码香叶基二磷酸合酶大亚基)。 在另一方面,提供已经用编码香叶基二磷酸合成酶大亚基的重组克隆载体和/或DNA序列转化,转染,感染和/或注射的修饰的宿主细胞。 在另一方面,本发明提供了分离的重组香叶基二磷酸合酶蛋白,其包含分离的重组香叶基二磷酸合酶大亚基蛋白和分离的重组香叶基二磷酸合酶小亚基蛋白。 因此,提供了重组表达香叶基二磷酸合成酶的系统和方法。