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1. 发明授权

US5374546A Process for stabilizing 1-methylhydantoinase, use and method of it for determining an analyte and agents suitable for this 失效
标题翻译：用于稳定1-甲基乙内酰脲酶的方法，其用于测定分析物的用途和方法以及适用于此的试剂
公开(公告)号：US5374546A
公开(公告)日：1994-12-20
申请号：US822176
申请日：1992-01-16
申请人： Rolf Nagel , Ulfert Deneke , Jurgen Mistele
发明人： Rolf Nagel , Ulfert Deneke , Jurgen Mistele
IPC分类号： C12N9/86 , C12N9/96 , C12Q1/26 , C12Q1/28 , C12Q1/34 , C12N9/78
CPC分类号： C12N9/86 , C12Q1/34 , G01N2333/986
摘要： The invention concerns a process for stabilizing the enzyme 1-methylhydantoinase (NMHase) which is characterized in that the NMHase is treated with divalent metal ions, a nucleoside triphosphate and a complexing agent which complexes these divalent metal ions. In addition the invention concerns the use of an enzyme stabilized in this way in a method for the determination of an analyte in which 1-methylhydantoin is converted, as well as a corresponding reagent.
摘要翻译：本发明涉及一种稳定酶1-甲基乙内酰脲酶（NMHase）的方法，其特征在于用二价金属离子，核苷三磷酸和与这些二价金属离子复合的络合剂处理NMHase。此外，本发明涉及以这种方式稳定的酶用于测定其中转化有1-甲基乙内酰脲的分析物以及相应试剂的方法。

2. 发明授权

US5162200A Method for determination of a peroxidate-acting substance with a diarylimidazole compound 失效
标题翻译：用二胺咪唑化合物测定过氧化物作用物质的方法
公开(公告)号：US5162200A
公开(公告)日：1992-11-10
申请号：US530661
申请日：1990-05-30
申请人： Hans-Peter Braun , Ulfert Deneke , Werner Guethlein , Rolf Nagel
发明人： Hans-Peter Braun , Ulfert Deneke , Werner Guethlein , Rolf Nagel
IPC分类号： G01N31/22 , C07D233/54 , C07D233/64 , C07D455/04 , C12Q1/26 , C12Q1/28 , C12Q1/50
CPC分类号： C07D233/64 , C12Q1/28 , C12Q1/50 , C12Q2326/00
摘要： Diarylimidazoles of the general formula: ##STR1## wherein R.sup.1 is a radical of the general formula: ##STR2## in which R.sup.3 and R.sup.6 are hydrogen atoms, R.sup.4 and R.sup.5, which can be the same or different, are lower alkyl radicals or R.sup.3 /R.sup.4 and/or R.sup.5 /R.sup.6 in each case together represent a lower alkylene radical and R.sup.2 is a lower alkyl radical, and/or of a salt thereof, are used as redox indicators in the colorimetric diarylimidazoles are not influenced by the presence of a SH group-containing substance. The peroxidate-acting substance can be used to determine the presence of creatine kinase.
摘要翻译：具有以下通式的二芳基咪唑：其中R 1是具有以下通式的基团：其中R 3和R 6是氢原子，R 4和R 5可以相同或不同，在各种情况下，低级烷基或R 3 / R 4和/或R 5 / R 6一起代表低级亚烷基，R 2为低级烷基，和/或其盐用作比色二芳基咪唑中的氧化还原指示剂不受影响通过存在含SH基团的物质。过氧化物作用物质可用于测定肌酸激酶的存在。

3. 发明授权

US5274095A Diarylimidazoles 失效
标题翻译：二芳基咪唑
公开(公告)号：US5274095A
公开(公告)日：1993-12-28
申请号：US928000
申请日：1992-08-11
申请人： Hans-Peter Braun , Ulfert Deneke , Werner Guethlein , Rolf Nagel
发明人： Hans-Peter Braun , Ulfert Deneke , Werner Guethlein , Rolf Nagel
IPC分类号： C07D233/54 , C12Q1/28 , C12Q1/50 , C07D401/04 , C07D233/64
CPC分类号： C07D233/64 , C12Q1/28 , C12Q1/50 , C12Q2326/00
摘要： Diarylimidazoles of the general formula: ##STR1## wherein R.sup.1 is a radical of the general formula: ##STR2## in which R.sup.3 and R.sup.6 are hydrogen atoms, R.sup.4 and R.sup.5, which can be the same or different, are lower alkyl radicals or R.sup.3 /R.sup.4 and /or R.sup.5 /R.sup.6 in each case together represent a lower alkylene radical and R.sup.2 is a lower alkyl radical, and/or of a salt thereof, are useful as redox indicators, particularly in the presence of a SH group-containing substance.
摘要翻译：具有以下通式的二芳基咪唑：其中R 1是具有以下通式的基团：其中R 3和R 6是氢原子，R 4和R 5可以相同或不同，在各种情况下，低级烷基或R 3 / R 4和/或R 5 / R 6一起代表低级亚烷基，R 2为低级烷基，和/或其盐可用作氧化还原指示剂，特别是在含SH组的物质。

4. 发明授权

US4876067A Multilayer test strip device with a dissolvable reagent layer 失效
标题翻译：具有可溶解试剂层的多层试片装置
公开(公告)号：US4876067A
公开(公告)日：1989-10-24
申请号：US89352
申请日：1987-08-25
申请人： Ulfert Deneke , Rolf Nagel , Anselm Rothe , Helmut Freitag
发明人： Ulfert Deneke , Rolf Nagel , Anselm Rothe , Helmut Freitag
IPC分类号： C12M1/34 , C12Q1/40 , G01N31/22 , G01N33/52
CPC分类号： G01N33/525 , Y10S435/805
摘要： The present invention provides a multi-layer test carrier (1) for the analytical determination of a component in a liquid sample, especially in a body fluid, having a first carrier layer (2) and a second carrier layer (10), at least one of which is transparent, a liquid-absorbing layer (3) being applied to the first carrier layer (2) and a reagent layer (11) containing at least one reagent being applied to the second carrier layer (10), the carrier layers being so arranged and fixed that the surfaces (3a, 11a) of the absorbing layer and of the reagent layer facing away from the carrier layer can be brought into contact with one another and can be pressed against one another, wherein the reagent layer (11) is soluble substantially without residue in the sample liquid and the take-up ability of the liquid-absorbing layer (3) for the sample liquid is so great that an amount of liquid absorbed therein suffices in order to dissolve the reagent layer free of residues so that substances contained in the reagent layer (11) pass over from the reagent layer (11) into the liquid-absorbing layer (3) when the two layers are pressed against one another.
摘要翻译：本发明提供了一种多层测试载体（1），用于分析确定具有第一载体层（2）和第二载体层（10）的液体样品中特别是体液中的组分，至少其中一个是透明的，液体吸收层（3）被施加到第一载体层（2）和试剂层（11），其中至少一个试剂被施加到第二载体层（10），载体层使得吸收层的表面（3a，11a）和试剂层背离载体层的表面（3a，11a）彼此接触并且可以彼此压靠，其中试剂层（11 ）在样品液体中基本上无残留地溶解，并且用于样品液体的液体吸收层（3）的吸收能力如此之大，使得其中吸收的液体量足以溶解没有残留物的试剂层使物质含量当所述两层被相互压靠时，所述试剂层（11）从所述试剂层（11）流入所述液体吸收层（3）。

5. 发明授权

US4603107A Reagent and process for the determination of the activity of the enzyme gamma-glutamyl transferase 失效
标题翻译：用于测定γ-谷氨酰转移酶酶活性的试剂和方法
公开(公告)号：US4603107A
公开(公告)日：1986-07-29
申请号：US532907
申请日：1983-09-16
申请人： Ulfert Deneke , Rolf Nagel , Walter Rittersdorf
发明人： Ulfert Deneke , Rolf Nagel , Walter Rittersdorf
IPC分类号： G01N33/50 , C12Q1/28 , C12Q1/48 , C12Q1/00 , C12Q1/36
CPC分类号： C12Q1/48 , Y10S435/805 , Y10S435/81
摘要： The present invention provides a process for the kinetic determination of .gamma.-glutamyl transferase activity by the reaction of a .gamma.-glutamylarylamide with the formation of an arylamine at a pH value of from 6 to 10, wherein the arylamine is simultaneously reacted with an aromatic amine or phenol appropriate as a coupling component and an appropriate oxidation agent to give a colored compound with an absorption maximum above 565 nm, the rate of formation of which is monitored photometrically and is used as a measure for the .gamma.-glutamyl transferase activity.The present invention also provides a reagent for the kinetic determination of .gamma.-GT activity, wherein it contains the following reagents:.gamma.-GT-arylamide:1-100 mmol/l..gamma.-glutamyl acceptor (glycylglycine):10-500 mmol/l.coupling component:1-500 mmol/l.oxidation agent:0.1-500 mmol/l.buffer:10-1000 mmol/l.
摘要翻译：本发明提供了一种通过γ-谷氨酰基芳基酰胺与pH6至10的芳胺形成反应来动态测定γ-谷氨酰转移酶活性的方法，其中芳胺与芳族胺同时反应或苯酚作为偶联组分和适当的氧化剂，得到最大吸收峰值高于565nm的着色化合物，其形成速率通过光度法监测并用作γ-谷氨酰转移酶活性的量度。本发明还提供用于γ-GT活性的动力学测定的试剂，其中它含有以下试剂：γ-GT-芳基酰胺：1-100mmol / l。 γ-谷氨酰受体（甘氨酰甘氨酸）：10-500mmol / l。偶联组分：1-500 mmol / l。氧化剂：0.1-500mmol / l。缓冲液：10-1000mmol / l。

6. 发明授权

US5786164A Method for separating non-high density lipoproteins from lipoprotein containing body fluid samples 失效
标题翻译：从含有脂蛋白的体液样品中分离非高密度脂蛋白的方法
公开(公告)号：US5786164A
公开(公告)日：1998-07-28
申请号：US661833
申请日：1996-06-11
申请人： Walter Rittersdorf , Ulfert Deneke , Gerhard Hiller , Hartmut Merdes , Klaus Buecker , Uwe Goebbert
发明人： Walter Rittersdorf , Ulfert Deneke , Gerhard Hiller , Hartmut Merdes , Klaus Buecker , Uwe Goebbert
IPC分类号： C12Q1/60 , G01N33/52 , G01N33/92
CPC分类号： C12Q1/60 , G01N33/526 , G01N33/92 , Y10T436/104165 , Y10T436/25125 , Y10T436/255
摘要： The invention relates to a method useful in separating non-high density lipoproteins, referred to as "non-HDLs", from biological fluids containing them. A porous carrier is provided which contains a non-HDL precipitating agent. One need only contact the sample of interest to the carrier for one minute or less, after which the precipitated non-HDL-s are no longer present in the sample being tested. The applications of the method include the ability to determine high density lipoproteins in the sample without interference from non-HDLs.
摘要翻译：本发明涉及从含有它们的生物液体中分离非高密度脂蛋白（称为“非HDL”）的方法。提供含有非HDL沉淀剂的多孔载体。只需要将感兴趣的样品与载体接触一分钟或更短时间，之后沉淀的非HDL-不再存在于待测样品中。该方法的应用包括测定样品中高密度脂蛋白而不受非HDL干扰的能力。

7. 发明授权

US06171849B2 Method for the determination of HDL cholesterol by means of a rapid diagnostic agent with an integrated fractionating step 有权
$Method for the determination of HDL cholesterol by means of a rapid diagnostic agent with an integrated fractionating step$
标题翻译：通过具有综合分馏步骤的快速诊断剂测定HDL胆固醇的方法
公开(公告)号：US06171849B2
公开(公告)日：2001-01-09
申请号：US09481664
申请日：2000-01-12
申请人： Walter Rittersdorf , Ulfert Deneke , Gerhard Hiller , Hartmut Merdes , Klaus Buecker , Uwe Goebbert
发明人： Walter Rittersdorf , Ulfert Deneke , Gerhard Hiller , Hartmut Merdes , Klaus Buecker , Uwe Goebbert
IPC分类号： C12M100
CPC分类号： C12Q1/60 , G01N33/526 , G01N33/92 , Y10T436/104165 , Y10T436/25125 , Y10T436/255
摘要： An apparatus comprising a first porous carrier and a second porous carrier for evaluating biological fluid samples is disclosed. The apparatus is used for separating non high density lipoprotein (non-HDL) from a lipoprotein in a body sample and for determining high density lipoprotein (HDL) cholesterol in a HDL and non high density lipoprotein (non-HDL) in a body sample.
摘要翻译：公开了一种包括用于评估生物流体样品的第一多孔载体和第二多孔载体的装置。该装置用于从身体样品中的脂蛋白分离非高密度脂蛋白（非HDL），并用于测定身体样品中HDL和非高密度脂蛋白（非HDL）中的高密度脂蛋白（HDL）胆固醇。

8. 发明授权

US5426030A Apparatus for determination of HDL cholesterol 失效
标题翻译：用于测定HDL胆固醇的装置
公开(公告)号：US5426030A
公开(公告)日：1995-06-20
申请号：US572875
申请日：1990-08-24
申请人： Walter Rittersdorf , Ulfert Deneke , Gerhard Hiller , Hartmut Merdes , Klaus Buecker , Uwe Goebbert
发明人： Walter Rittersdorf , Ulfert Deneke , Gerhard Hiller , Hartmut Merdes , Klaus Buecker , Uwe Goebbert
IPC分类号： C12Q1/60 , G01N33/52 , G01N33/92 , G01N21/00
CPC分类号： C12Q1/60 , G01N33/526 , G01N33/92 , Y10T436/104165 , Y10T436/25125 , Y10T436/255
摘要： The invention relates to an apparatus and a method useful in separating non-high density lipoproteins, referred to as "non-HDLs", from biological fluids containing them. A porous carrier is provided which contains a non-HDL precipitating agent. One need only contact the sample of interest to the carrier for one minute or less, after which the precipitated non-HDLs are no longer present in the sample being tested. The applications of the method include the ability to determine high density lipoproteins in the sample without interference from non-HDLs.
摘要翻译：本发明涉及一种用于从含有它们的生物液体中分离非高密度脂蛋白（被称为“非HDL”）的装置和方法。提供含有非HDL沉淀剂的多孔载体。仅需要将感兴趣的样品与载体接触一分钟或更短时间，之后沉淀的非HDL不再存在于待测样品中。该方法的应用包括测定样品中高密度脂蛋白而不受非HDL干扰的能力。

9. 发明授权

US4966855A New redox indicators 失效
标题翻译：新的氧化还原指标
公开(公告)号：US4966855A
公开(公告)日：1990-10-30
申请号：US751367
申请日：1985-07-02
申请人： Ulfert Deneke , Werner Guthlein , Wolfgang Weckerle , Hans Wielinger
发明人： Ulfert Deneke , Werner Guthlein , Wolfgang Weckerle , Hans Wielinger
IPC分类号： C07D263/32 , C07D277/22 , C07D277/28 , C07D277/72 , C07D413/04 , C07D413/06 , C07D413/14 , C07D455/04 , C07F9/60 , C07F9/653 , C07F9/655 , C12Q1/26 , C12Q1/28 , G01N33/50 , G01N33/52
CPC分类号： C07D413/04 , C07D263/32 , C07D277/28 , C07D413/06 , C07D413/14 , C07D455/04 , C07F9/60 , C07F9/653 , C07F9/65505 , C12Q1/26 , C12Q1/28 , G01N33/525 , C12Q2326/00 , Y10S435/807 , Y10S436/904 , Y10T436/206664
摘要： The invention provides new redox indicators and uses for these redox compounds (I): ##STR1## Wherein X is oxygen or sulphur, R.sub.1 is julolidine or tetrahydroquinoline, which can carry an alkyl radical on the nitrogen atom which, in turn, can be substituted by a sulphuric, phosphonic or carboxylic acid residues, or is (II); wherein R.sub.4 is hydroxyl or a mono- or dialkylated amino group, R.sub.5 and R.sub.6, which can be the same or different, are hydrogen, alkyl or alkoxy, R.sub.2 is hydrogen or alkyl, julolidine or tetrahydroquinoline, which can carry an alkyl on the nitrogen atom which alkyl can be substituted by sulphuric, phosphonic or carboxylic acids, or is III; wherein R.sub.5 and R.sub.6 are the same as in R.sub.1 and R'.sub.4 is hydroxyl, an amino or a mono-or dialkylated amino group, whereby the alkyl radicals can be substituted one or more times by hydroxyl, alkoxy, halogen, morpholine or a sulphuric, carboxylic or phosphonic acid residue, which acids can also be esterified, and R.sub.3 is the same as R.sub.2 or is an alkyl substituted by hydroxyl, alkoxy, dialkylamino or phenyl, or is cycloalkyl, phenyl or pyridyl; as well as the salts thereof.
摘要翻译：本发明为这些氧化还原化合物（I）提供了新的氧化还原指示剂和用途：其中X是氧或硫，R1是可乐兰或四氢喹啉，其可以携带或者是（II）的氮原子上的烷基，其又可以被硫酸，膦酸或羧酸残基取代。其中R4是羟基或单烷基化或二烷基化氨基，可以相同或不同的R5和R6是氢，烷基或烷氧基，R2是氢或可以在氮上带有烷基的烷基，多环糊精或四氢喹啉这个烷基可以被硫酸，膦酸或羧酸取代，或者是III; 其中R5和R6与R1相同，R'4是羟基，氨基或单或二烷基化氨基，其中烷基可以被羟基，烷氧基，卤素，吗啉或硫酸取代一次或多次羧酸或膦酸残基，该酸也可以被酯化，R3与R2相同或是被羟基，烷氧基，二烷基氨基或苯基取代的烷基，或是环烷基，苯基或吡啶基; 以及它们的盐。

10. 发明授权

US4591553A Process and analytical agent for the determination of the activity of glutamate-oxalacetate-transaminase 失效
标题翻译：用于测定谷氨酸 - 乙酸肝素 - 转氨酶活性的方法和分析试剂
公开(公告)号：US4591553A
公开(公告)日：1986-05-27
申请号：US500111
申请日：1983-06-01
申请人： Ulfert Deneke , Wolfgang Gruber , Hans-Georg Batz
发明人： Ulfert Deneke , Wolfgang Gruber , Hans-Georg Batz
IPC分类号： G01N33/52 , C12Q1/00 , C12Q1/26 , C12Q1/48 , C12Q1/52
CPC分类号： C12Q1/52 , Y10S435/805
摘要： The present invention provides a process for the determination of the activity of glutamateoxalacetate-transaminase (GOT) in aqueous solutions in which(a) .alpha.-ketoglutarate is reacted with alanine-sulphinic acid, catalyzed by GOT, to give pyruvate, sulphur dioxide and glutamate,(b) the resultant pyruvate is reacted with phosphate and oxygen, catalyzed by pyruvate oxidase, to give acetyl phosphate, carbon dioxide and hydrogen peroxide,(c) the hydrogen peroxide and a chromogen is reacted, catalyzed by a peroxidase, to give a dyestuff and water and(d) the amount of dyestuff formed is determined, wherein the chromogen used is N-(3'-sulphonyl-4'-hydroxy-5'-chlorophenyl)-4-aminoantipyrine (SHYC-AA), 4,5-bis-[4-dimethylaminophenyl]-2-[3,5-dimethoxy-4-hydroxyphenyl]-imidazole (B-DDH-imidazole) or a related substance.The present invention also provides an analytical agent for the determination of the activity of GOT, comprising .alpha.-ketoglutarate, alanine-sulphinic acid, a chromogen, peroxidase, pyruvate oxidase, soluble phosphate, buffer and water, wherein the chromogen is N-(3'-sulphonyl-4'-hydroxy-5'-chlorophenyl)-4-aminoantipyrine (SHYC-AA) or 4,5-bis-[4-dimethylaminophenyl]-2-[3,5-dimethoxy-4-hydroxyphenyl]-imidazole (B-DDH-imidazole).
摘要翻译：本发明提供了一种测定谷氨酸酰乙酰转氨酶（GOT）在水溶液中的活性的方法，其中（a）α-酮戊二酸与由GOT催化的丙氨酸 - 亚磺酸反应得到丙酮酸，二氧化硫和谷氨酸，（b）所得丙酮酸盐与磷酸盐和氧气反应，由丙酮酸氧化酶催化，得到乙酰磷酸盐，二氧化碳和过氧化氢，（c）过氧化氢和色原反应，由过氧化物酶催化，得到染料和水，（d）确定形成的染料的量，其中使用的色原是N-（3'-磺酰基-4'-羟基-5'-氯苯基）-4-氨基安替比林（SHYC-AA） 5-双 - [4-二甲基氨基苯基] -2- [3,5-二甲氧基-4-羟基苯基] - 咪唑（B-DDH-咪唑）或相关物质。本发明还提供了用于测定GOT的活性的分析剂，其包括α-酮戊二酸，丙氨酸 - 亚磺酸，色原，过氧化物酶，丙酮酸氧化酶，可溶性磷酸盐，缓冲液和水，其中色原为N-（3 4-磺基-4'-羟基-5'-氯苯基）-4-氨基安替比林（SHYC-AA）或4,5-双 - [4-二甲基氨基苯基] -2- [3,5-二甲氧基-4-羟基苯基] 咪唑（B-DDH-咪唑）。

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