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1. 发明申请

US20060246514A1 MASS SPECTROMETRIC IMMUNOASSAY 失效
标题翻译：大量光谱免疫
公开(公告)号：US20060246514A1
公开(公告)日：2006-11-02
申请号：US11459471
申请日：2006-07-24
申请人： Randall Nelson , Peter Williams , Jennifer Krone
发明人： Randall Nelson , Peter Williams , Jennifer Krone
IPC分类号： G01N33/53
CPC分类号： G01N24/00 , C12Q1/6816 , C12Q1/6872 , G01N33/5302 , G01N33/54373 , G01N33/6851 , Y10T436/24
摘要： Rapid mass spectrometric immunoassay methods for detecting and/or quantifying antibody and antigen analytes utilizing affinity capture to isolate the analytes and internal reference species (for quantification) followed by mass spectrometric analysis of the isolated analyte/internal reference species. Quantification is obtained by normalizing and calibrating obtained mass spectrum against the mass spectrum obtained for an antibody/antigen of known concentration.
摘要翻译：用于利用亲和捕获来检测和/或定量抗体和抗原分析物以分离分析物和内部参考物质（用于定量），随后对分离的分析物/内部参考物质进行质谱分析的快速质谱免疫测定方法。通过对获得的已知浓度的抗体/抗原的质谱进行标准化和校准获得的质谱来获得定量。

2. 发明申请

US20060270062A1 MASS SPECTROMETRIC IMMUNOASSAY 审中-公开
公开(公告)号：US20060270062A1
公开(公告)日：2006-11-30
申请号：US11462504
申请日：2006-08-04
申请人： Randall Nelson , Peter Williams , Jennifer Krone
发明人： Randall Nelson , Peter Williams , Jennifer Krone
IPC分类号： G01N33/543 , G01N33/00
CPC分类号： G01N24/00 , C12Q1/6816 , C12Q1/6872 , G01N33/5302 , G01N33/54373 , G01N33/6851 , Y10T436/24
摘要： Rapid mass spectrometric immunoassay methods for detecting and/or quantifying antibody and antigen analytes utilizing affinity capture to isolate the analytes and internal reference species (for quantification) followed by mass spectrometric analysis of the isolated analyte/internal reference species. Quantification is obtained by normalizing and calibrating obtained mass spectrum against the mass spectrum obtained for an antibody/antigen of known concentration.

3. 发明申请

US20050153311A1 Mass spectrometric immunoassay analysis of specific proteins and variants present in various biological fluids 失效
标题翻译：对各种生物液体中存在的特定蛋白质和变体的质谱免疫分析
公开(公告)号：US20050153311A1
公开(公告)日：2005-07-14
申请号：US10958867
申请日：2004-10-04
申请人： Randall Nelson , Peter Williams , Jennifer Krone
发明人： Randall Nelson , Peter Williams , Jennifer Krone
IPC分类号： C12Q1/68 , G01N33/53 , G01N33/543
CPC分类号： C12Q1/6816 , C12Q1/6872 , G01N33/5302 , G01N33/54373 , Y10T436/24
摘要： Presented herein is the construction of pipettor tips (termed MSIA-Tips) containing porous solid supports that are constructed, covalently derivatized with affinity ligand, and used to extract specific proteins and their variants from various biological fluids by repeatedly flowing the fluids through the MSIA-Tips. A second protein species (a mass-shifted variant of the targeted protein doped into the samples at a constant concentration) is co-extracted with the endogenous protein and variants and is used as a quantitative internal reference standard (IRS). Nonspecifcally bound compounds are rinsed from the MSIA-Tip using a series of buffer and water rinses, after which the wild type protein, protein variants and the IRS are eluted from the MSIA-Tips directly onto a target in preparation for analysis such as MALDI-TOF. Mass spectrometry of the eluted sample then follows with the retained proteins identified via accurate molecular mass determination. Protein and variant levels are determined via a quantitative method in which the protein/variant signals are normalized to the signal of the IRS and the values compared to a working curve constructed from samples containing known concentrations of the protein or variants.
摘要翻译：这里提出的是构建含有多孔固体支持物的移液器尖端（称为MSIA-Tips），其构建为用亲和配体共价衍生，并且用于通过重复地使流体流过MSIA-DNA来从各种生物流体中提取特异性蛋白质及其变体，提示。将第二种蛋白质物种（以恒定浓度掺入样品中的靶向蛋白质的质量转移变体）与内源蛋白质和变体共萃取并用作定量内部参照标准品（IRS）。使用一系列缓冲液和水冲洗从MSIA-Tip中冲洗非特异性结合的化合物，之后将野生型蛋白质，蛋白质变体和IRS直接从MSIA-Tip中洗脱到靶上用于分析，如MALDI- TOF。洗脱的样品的质谱随后通过精确的分子量测定鉴定的保留的蛋白质。通过定量方法测定蛋白质和变体水平，其中将蛋白质/变体信号归一化为IRS的信号，并且该值与由含有已知浓度的蛋白质或变体的样品构建的工作曲线相比较。

4. 发明授权

US09096898B2 Method of determining the nucleotide sequence of oligonucleotides and DNA molecules 有权
公开(公告)号：US09096898B2
公开(公告)日：2015-08-04
申请号：US12969872
申请日：2010-12-16
申请人： Peter Williams , Thomas J. Taylor , Daniel J. B. Williams , Ian Gould , Mark A. Hayes
发明人： Peter Williams , Thomas J. Taylor , Daniel J. B. Williams , Ian Gould , Mark A. Hayes
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q1/6825 , C12Q2533/101 , C12Q2563/103 , C12Q2563/107 , C12Q2563/116 , C12Q2565/301 , C12Q2565/629 , G01N33/573 , G01N33/6863
摘要： The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

5. 发明申请

US20140030142A1 DECONTAMINATION OF ENCLOSED SPACE USING GASEOUS CHLORINE DIOXIDE 有权
标题翻译：使用气体二氧化氯减少封闭空间
公开(公告)号：US20140030142A1
公开(公告)日：2014-01-30
申请号：US13466708
申请日：2012-05-08
申请人： John Y. Mason , Peter Williams , Maggie Trabeau
发明人： John Y. Mason , Peter Williams , Maggie Trabeau
IPC分类号： A61L2/20
CPC分类号： A61L9/00 , A61L2/20 , A61L2/22 , A61L9/015 , A61L9/03 , A61L9/04 , A61L9/122 , A61L9/145 , A61L2202/25 , A61L2209/22 , B01F5/20 , C01B11/022 , F24F6/00
摘要： A method for gas phase application of chlorine dioxide within an enclosed volume that includes the steps of: climatizing the enclosed volume to achieve a relative humidity (RH) in the range of about 5% to about 56%; generating chlorine dioxide gas; and introducing the chlorine dioxide gas under specified conditions of chlorine dioxide gas concentration and contact time, the specified conditions being effective to eliminate contaminants within the closed volume, and further to mitigate corrosion within the enclosed volume during the gas phase application.
摘要翻译：一种在封闭容积内气相施用二氧化氯的方法，包括以下步骤：使封闭体积气化，以达到约5％至约56％范围内的相对湿度（RH）; 产生二氧化氯气体; 并在二氧化氯气体浓度和接触时间的规定条件下引入二氧化氯气体，规定的条件有效地消除封闭体积内的污染物，并进一步减轻在气相施加期间封闭体积内的腐蚀。

6. 发明申请

US20130233067A1 TIRE UNIFORMITY TESTING SYSTEM 有权
标题翻译：轮胎均匀测试系统
公开(公告)号：US20130233067A1
公开(公告)日：2013-09-12
申请号：US13885850
申请日：2011-11-22
申请人： James Wollbrinck , Richard R. Matuszny , Barry Cargould , Peter Williams
发明人： James Wollbrinck , Richard R. Matuszny , Barry Cargould , Peter Williams
IPC分类号： G01M17/02 , B60C25/00
CPC分类号： G01M17/02 , B60C25/002 , G01M17/021 , Y10T29/49815 , Y10T29/49826
摘要： An apparatus for changing rim sets at a testing station of a tire uniformity testing system that includes a storage unit for storing a plurality of rim set assemblies. A transfer arm is reciprocally movable and includes a gripper mechanism for gripping a rim set assembly and moving it from a storage position to a position at which rims forming part of the rim set assembly are aligned with opposed spindles at the testing station. The rim set assembly includes a bracket having structure engageable by the transfer arm and at least one of the rims forming part of the assembly includes a locking member rotatable between a first position and a second position. The bracket includes a structure for immobilizing the rim to inhibit relative rotation between the rim and the bracket and further includes a lever mechanism for moving the locking member from its first position to its second position in order to engage retaining members forming part of the bracket. The lever mechanism moves the locking member from its second position to its first position when the rim is aligned with an associated spindle in order to engage other retaining members forming part of the spindle. The rim is released from the bracket as the locking member moves to engage the other retaining members. After release, the bracket moves to a remote position spaced from the testing station. To remove the rim sets, the steps for attaching rims to the spindles are reversed in order to release the rims from the spindles while simultaneously engaging the bracket. The resulting rim set assembly is moved to a remote position defined by a storage unit that stores a plurality of rim set assemblies. The disclosed locking member and associated rim can be used to facilitate the manual installation of a test rim to a spindle.
摘要翻译：一种用于在轮胎均匀性测试系统的测试站处改变轮辋组件的装置，其包括用于存储多个轮辋组件的存储单元。传送臂是可往复运动的，并且包括夹持机构，用于夹持轮圈组件并将其从存放位置移动到形成轮圈组件的一部分的轮缘与测试台处的相对的主轴对准的位置。轮辋组件包括具有可由传送臂接合的结构的支架，并且构成组件的一部分的至少一个轮辋包括可在第一位置和第二位置之间旋转的锁定构件。支架包括用于固定轮缘以阻止轮缘和支架之间的相对旋转的结构，并且还包括杠杆机构，用于将锁定构件从其第一位置移动到其第二位置，以便接合构成支架的一部分的保持构件。当边缘与相关联的主轴对准时，杠杆机构将锁定构件从其第二位置移动到其第一位置，以便接合形成主轴的一部分的其它保持构件。当锁定构件移动以与其它保持构件接合时，边缘从支架释放。释放后，支架移动到与测试站隔开的远程位置。为了拆下轮辋组，将轮辋连接到主轴上的步骤是相反的，以便在同时接合支架的同时将轮辋从主轴上松开。所得到的轮圈组件移动到由存储多个轮圈组件的存储单元限定的远程位置。所公开的锁定构件和相关联的边缘可以用于便于将试验轮辋手动安装到主轴。

7. 发明授权

US08263365B2 Method of determining the nucleotide sequence of oligonucleotides and DNA molecules 有权
公开(公告)号：US08263365B2
公开(公告)日：2012-09-11
申请号：US13008392
申请日：2011-01-18
申请人： Peter Williams , Thomas J. Taylor , Daniel J. B. Williams , Ian Gould , Mark A. Hayes
发明人： Peter Williams , Thomas J. Taylor , Daniel J. B. Williams , Ian Gould , Mark A. Hayes
IPC分类号： C12P19/34 , C12Q1/68 , C07H21/00 , C07H21/02
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q1/6825 , C12Q2533/101 , C12Q2563/103 , C12Q2563/107 , C12Q2563/116 , C12Q2565/301 , C12Q2565/629 , G01N33/573 , G01N33/6863
摘要： The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

8. 发明申请

US20120171782A1 MASS SPECTROMETRIC IMMUNOASSAY 失效
标题翻译：大量光谱免疫
公开(公告)号：US20120171782A1
公开(公告)日：2012-07-05
申请号：US13421633
申请日：2012-03-15
申请人： Randall W. Nelson , Peter Williams , Jennifer Reeve Krone
发明人： Randall W. Nelson , Peter Williams , Jennifer Reeve Krone
IPC分类号： G01N33/553 , G01N33/545 , G01N33/544 , G01N33/548 , G01N33/543 , G01N33/552
CPC分类号： G01N24/00 , C12Q1/6816 , C12Q1/6872 , G01N33/5302 , G01N33/54373 , G01N33/6851 , Y10T436/24
摘要： Rapid mass spectrometric immunoassay methods for detecting and/or quantifying antibody and antigen analytes utilizing affinity capture to isolate the analytes and internal reference species (for quantification) followed by mass spectrometric analysis of the isolated analyte/internal reference species. Quantification is obtained by normalizing and calibrating obtained mass spectrum against the mass spectrum obtained for an antibody/antigen of known concentration.
摘要翻译：用于利用亲和捕获来检测和/或定量抗体和抗原分析物以分离分析物和内部参考物质（用于定量），随后对分离的分析物/内部参考物质进行质谱分析的快速质谱免疫测定方法。通过对获得的已知浓度的抗体/抗原的质谱进行标准化和校准获得的质谱来获得定量。

9. 发明申请

US20110183323A1 METHOD OF DETERMINING THE NUCLEOTIDE SEQUENCE OF OLIGONUCLEOTIDES AND DNA MOLECULES 有权
公开(公告)号：US20110183323A1
公开(公告)日：2011-07-28
申请号：US13008392
申请日：2011-01-18
申请人： Peter Williams , Thomas J. Taylor , Daniel J. B. Williams , Ian Gould , Mark A. Hayes
发明人： Peter Williams , Thomas J. Taylor , Daniel J. B. Williams , Ian Gould , Mark A. Hayes
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q1/6825 , C12Q2533/101 , C12Q2563/103 , C12Q2563/107 , C12Q2563/116 , C12Q2565/301 , C12Q2565/629 , G01N33/573 , G01N33/6863
摘要： The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

10. 发明授权

US07640667B2 Hand tools 失效
标题翻译：手工具
公开(公告)号：US07640667B2
公开(公告)日：2010-01-05
申请号：US11627292
申请日：2007-01-25
申请人： Nathan Pollock , James Collins , Lee Rodezno , Peter Williams
发明人： Nathan Pollock , James Collins , Lee Rodezno , Peter Williams
IPC分类号： B26B13/28 , B26B13/00 , B25B7/06
CPC分类号： A01G3/02 , B26B13/28
摘要： A pruner has a movable handle/blade assembly pivotally coupled to a static handle/blade assembly by a pivot assembly carried by the static handle. The pivot assembly further has a bush within the static handle and on which the movable handle/blade assembly is pivotally mounted, a spring for pivotally biasing the movable handle/blade assembly to an open position being retained by the pivot assembly within the static handle. Both the movable blade and static blade are mounted on the bush and are clamped laterally between two separate parts of the bush which are assembled together in screw threaded engagement, and the two blades are supported from the bush by a bearing ring of low friction material.
摘要翻译：修剪器具有通过由静态手柄承载的枢轴组件枢转地联接到静态手柄/刀片组件的可动手柄/刀片组件。枢轴组件还具有在静态手柄内的衬套，并且可动手柄/刀片组件可枢转地安装在该衬套上，弹簧用于将可移动手柄/刀片组件枢转地偏置到由静止手柄内的枢转组件保持的打开位置。可移动刀片和静态刀片都安装在衬套上，并且被侧向地夹紧在衬套的两个分开的部件之间，这些部件以螺纹接合组装在一起，并且两个刀片通过低摩擦材料的轴承环从衬套支撑。

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