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1. 发明授权

US07396687B2 Mass spectrometric immunoassay analysis of specific proteins and variants present in various biological fluids 失效
标题翻译：对各种生物液体中存在的特定蛋白质和变体的质谱免疫分析
公开(公告)号：US07396687B2
公开(公告)日：2008-07-08
申请号：US10958867
申请日：2004-10-04
申请人： Randall W. Nelson , Peter Williams , Jennifer Reeve Krone
发明人： Randall W. Nelson , Peter Williams , Jennifer Reeve Krone
IPC分类号： G01N24/00
CPC分类号： C12Q1/6816 , C12Q1/6872 , G01N33/5302 , G01N33/54373 , Y10T436/24
摘要： Presented herein is the construction of pipettor tips (termed MSIA-Tips) containing porous solid supports that are constructed, covalently derivatized with affinity ligand, and used to extract specific proteins and their variants from various biological fluids by repeatedly flowing the fluids through the MSIA-Tips. A second protein species (a mass-shifted variant of the targeted protein doped into the samples at a constant concentration) is co-extracted with the endogenous protein and variants and is used as a quantitative internal reference standard (IRS). Nonspecifcally bound compounds are rinsed from the MSIA-Tip using a series of buffer and water rinses, after which the wild type protein, protein variants and the IRS are eluted from the MSIA-Tips directly onto a target in preparation for analysis such as MALDI-TOF. Mass spectrometry of the eluted sample then follows with the retained proteins identified via accurate molecular mass determination. Protein and variant levels are determined via a quantitative method in which the protein/variant signals are normalized to the signal of the IRS and the values compared to a working curve constructed from samples containing known concentrations of the protein or variants.
摘要翻译：这里提出的是构建含有多孔固体支持物的移液器尖端（称为MSIA-Tips），其构建为用亲和配体共价衍生，并且用于通过重复地使流体流过MSIA-DNA来从各种生物流体提取特定蛋白质及其变体，提示。将第二种蛋白质物种（以恒定浓度掺入样品中的靶向蛋白质的质量转移变体）与内源蛋白质和变体共萃取并用作定量内部参照标准品（IRS）。使用一系列缓冲液和水冲洗从MSIA-Tip中冲洗非特异性结合的化合物，之后将野生型蛋白质，蛋白质变体和IRS直接从MSIA-Tip中洗脱到靶上用于分析，如MALDI- TOF。洗脱的样品的质谱随后通过精确的分子量测定鉴定的保留的蛋白质。通过定量方法测定蛋白质和变体水平，其中将蛋白质/变体信号归一化为IRS的信号，并且该值与由含有已知浓度的蛋白质或变体的样品构建的工作曲线相比较。

2. 发明授权

US06974704B2 Mass spectrometric immunoassay 失效
标题翻译：质谱免疫测定
公开(公告)号：US06974704B2
公开(公告)日：2005-12-13
申请号：US09808275
申请日：2001-03-14
申请人： Randall W. Nelson , Peter Williams , Jennifer Reeve Krone
发明人： Randall W. Nelson , Peter Williams , Jennifer Reeve Krone
IPC分类号： C12Q1/68 , G01N33/53 , G01N33/543 , G01N24/00
CPC分类号： G01N24/00 , C12Q1/6816 , C12Q1/6872 , G01N33/5302 , G01N33/54373 , G01N33/6851 , Y10T436/24
摘要： Rapid mass spectrometric immunoassay methods for detecting and/or quantifying antibody and antigen analytes utilizing affinity capture to isolate the analytes and internal reference species (for quantification) followed by mass spectrometric analysis of the isolated analyte/internal reference species. Quantification is obtained by normalizing and calibrating obtained mass spectrum against the mass spectrum obtained for an antibody/antigen of known concentration.
摘要翻译：用于利用亲和捕获来检测和/或定量抗体和抗原分析物以分离分析物和内部参考物质（用于定量），随后对分离的分析物/内部参考物质进行质谱分析的快速质谱免疫测定方法。通过对获得的已知浓度的抗体/抗原的质谱进行标准化和校准获得的质谱来获得定量。

3. 发明授权

US07399641B2 Mass spectrometric immunoassay 失效
标题翻译：质谱免疫测定
公开(公告)号：US07399641B2
公开(公告)日：2008-07-15
申请号：US10904467
申请日：2004-11-11
申请人： Randall W. Nelson , Peter Williams , Jennifer Reeve Krone
发明人： Randall W. Nelson , Peter Williams , Jennifer Reeve Krone
IPC分类号： G01N24/00
CPC分类号： G01N24/00 , C12Q1/6816 , C12Q1/6872 , G01N33/5302 , G01N33/54373 , G01N33/6851 , Y10T436/24
摘要： Rapid mass spectrometric immunoassay methods for detecting and/or quantifying antibody and antigen analytes utilizing affinity capture to isolate the analytes and internal reference species (for quantification) followed by mass spectrometric analysis of the isolated analyte/internal reference species. Quantification is obtained by normalizing and calibrating obtained mass spectrum against the mass spectrum obtained for an antibody/antigen of known concentration.
摘要翻译：用于利用亲和捕获来检测和/或定量抗体和抗原分析物以分离分析物和内部参考物质（用于定量），随后对分离的分析物/内部参考物质进行质谱分析的快速质谱免疫测定方法。通过对获得的已知浓度的抗体/抗原的质谱进行标准化和校准获得的质谱来获得定量。

4. 发明授权

US08486713B2 Mass spectrometric immunoassay 失效
标题翻译：质谱免疫测定
公开(公告)号：US08486713B2
公开(公告)日：2013-07-16
申请号：US13421633
申请日：2012-03-15
申请人： Randall W. Nelson , Peter Williams , Jennifer Reeve Krone
发明人： Randall W. Nelson , Peter Williams , Jennifer Reeve Krone
IPC分类号： G01N24/00
CPC分类号： G01N24/00 , C12Q1/6816 , C12Q1/6872 , G01N33/5302 , G01N33/54373 , G01N33/6851 , Y10T436/24
摘要： Rapid mass spectrometric immunoassay methods for detecting and/or quantifying antibody and antigen analytes utilizing affinity capture to isolate the analytes and internal reference species (for quantification) followed by mass spectrometric analysis of the isolated analyte/internal reference species. Quantification is obtained by normalizing and calibrating obtained mass spectrum against the mass spectrum obtained for an antibody/antigen of known concentration.
摘要翻译：用于利用亲和捕获来检测和/或定量抗体和抗原分析物以分离分析物和内部参考物质（用于定量），随后对分离的分析物/内部参考物质进行质谱分析的快速质谱免疫测定方法。通过对获得的已知浓度的抗体/抗原的质谱进行标准化和校准获得的质谱来获得定量。

5. 发明授权

US07303888B2 Mass spectrometric immunoassay 失效
标题翻译：质谱免疫测定
公开(公告)号：US07303888B2
公开(公告)日：2007-12-04
申请号：US11459471
申请日：2006-07-24
申请人： Randall W Nelson , Peter Williams , Jennifer Reeve Krone
发明人： Randall W Nelson , Peter Williams , Jennifer Reeve Krone
IPC分类号： G01N33/53
CPC分类号： G01N24/00 , C12Q1/6816 , C12Q1/6872 , G01N33/5302 , G01N33/54373 , G01N33/6851 , Y10T436/24
摘要： Rapid mass spectrometric immunoassay methods for detecting and/or quantifying antibody and antigen analytes utilizing affinity capture to isolate the analytes and internal reference species (for quantification) followed by mass spectrometric analysis of the isolated analyte/internal reference species. Quantification is obtained by normalizing and calibrating obtained mass spectrum against the mass spectrum obtained for an antibody/antigen of known concentration.
摘要翻译：用于利用亲和捕获来检测和/或定量抗体和抗原分析物以分离分析物和内部参考物质（用于定量），随后对分离的分析物/内部参考物质进行质谱分析的快速质谱免疫测定方法。通过对获得的已知浓度的抗体/抗原的质谱进行标准化和校准获得的质谱来获得定量。

6. 发明申请

US20120171782A1 MASS SPECTROMETRIC IMMUNOASSAY 失效
标题翻译：大量光谱免疫
公开(公告)号：US20120171782A1
公开(公告)日：2012-07-05
申请号：US13421633
申请日：2012-03-15
申请人： Randall W. Nelson , Peter Williams , Jennifer Reeve Krone
发明人： Randall W. Nelson , Peter Williams , Jennifer Reeve Krone
IPC分类号： G01N33/553 , G01N33/545 , G01N33/544 , G01N33/548 , G01N33/543 , G01N33/552
CPC分类号： G01N24/00 , C12Q1/6816 , C12Q1/6872 , G01N33/5302 , G01N33/54373 , G01N33/6851 , Y10T436/24
摘要： Rapid mass spectrometric immunoassay methods for detecting and/or quantifying antibody and antigen analytes utilizing affinity capture to isolate the analytes and internal reference species (for quantification) followed by mass spectrometric analysis of the isolated analyte/internal reference species. Quantification is obtained by normalizing and calibrating obtained mass spectrum against the mass spectrum obtained for an antibody/antigen of known concentration.
摘要翻译：用于利用亲和捕获来检测和/或定量抗体和抗原分析物以分离分析物和内部参考物质（用于定量），随后对分离的分析物/内部参考物质进行质谱分析的快速质谱免疫测定方法。通过对获得的已知浓度的抗体/抗原的质谱进行标准化和校准获得的质谱来获得定量。

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