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    • 5. 发明授权
    • Nucleic acids encoding fusion peptides
    • 编码融合肽的核酸
    • US07348423B2
    • 2008-03-25
    • US10969624
    • 2004-10-20
    • Eva HoessThomas MeierGabriele PestlinFriedrich PoppKlaus ReichertRainer SchmuckBernd SchneidingerChristoph SeidelWilhelm Tischer
    • Eva HoessThomas MeierGabriele PestlinFriedrich PoppKlaus ReichertRainer SchmuckBernd SchneidingerChristoph SeidelWilhelm Tischer
    • A61K39/21
    • C07K14/005C07K2319/00C12N2740/15022C12N2740/16122C12P21/02
    • A process is disclosed for the production of an antifusogenic peptide by producing a fusion peptide of a length of about 14 to 70 amino acids in a prokaryotic host cell, comprising the steps, under such conditions that inclusion bodies of said fusion peptide are formed, of: (a) expressing in said host cell a nucleic acid encoding said fusion peptide consisting of a first peptide which is an antifusogenic peptide of a length of about 10 to 50 amino acids and a second peptide of a length of about 4 to 30 amino acids, said first peptide being N-terminally linked to said second peptide; (b) cultivating said host cell to produce said inclusion bodies; and (c) recovering said antifusogenic peptide from said inclusion bodies, wherein said recovered antifusogenic peptide consists of said fusion peptide or a peptide comprising the antifusogenic peptide of about 10 to 50 amino acids and which is a fragment cleaved from said fusion peptide. Inclusion bodies of the peptides are disclosed. Also disclosed is a nucleic acid encoding the fusion peptide consisting of a first peptide which is an antifusogenic peptide selected from the group of SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, and said sequences further consisting of glycine at the C terminal end, N-terminally linked to the second peptide.
    • 公开了通过在原核宿主细胞中产生约14至70个氨基酸长度的融合肽来生产抗融合肽的方法,包括以下步骤:在形成所述融合肽的包涵体的条件下, (a)在所述宿主细胞中表达编码所述融合肽的核酸,所述融合肽由长度为约10至50个氨基酸的抗融合肽的第一肽和长度为约4-30个氨基酸的第二个肽组成 所述第一肽与所述第二肽N-末端连接; (b)培养所述宿主细胞以产生所述包涵体; 和(c)从所述包涵体中回收所述抗融合肽,其中所述回收的抗融合肽由所述融合肽或包含约10至50个氨基酸的抗融合肽的肽组成,并且是从所述融合肽切割的片段。 公开了肽的包涵体。 还公开了编码由第一肽组成的融合肽的核酸,第一肽是选自SEQ ID NO:7,SEQ ID NO:8,SEQ ID NO:9,SEQ ID NO:10的抗融合肽, 所述序列进一步由C末端的甘氨酸组成,N-末端与第二肽连接。