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1. 发明授权

US06858410B2 Process for making antifusogenic fusion peptides that form inclusion bodies 失效
标题翻译：制造形成包涵体的抗融合融合肽的方法
公开(公告)号：US06858410B2
公开(公告)日：2005-02-22
申请号：US10158742
申请日：2002-05-30
申请人： Eva Hoess , Thomas Meier , Gabriele Pestlin , Friedrich Popp , Klaus Reichert , Rainer Schmuck , Bernd Schneidinger , Christoph Seidel , Wilhelm Tischer
发明人： Eva Hoess , Thomas Meier , Gabriele Pestlin , Friedrich Popp , Klaus Reichert , Rainer Schmuck , Bernd Schneidinger , Christoph Seidel , Wilhelm Tischer
IPC分类号： C12N15/09 , A61K38/00 , A61P31/12 , A61P31/18 , C07K14/155 , C07K14/16 , C12N1/21 , C12N15/62 , C12P21/02 , C12P21/00
CPC分类号： C07K14/005 , C07K2319/00 , C12N2740/15022 , C12N2740/16122 , C12P21/02
摘要： A process is provided for producing an antifusogenic peptide by producing a fusion peptide of from about 14 amino acids up to 70 amino acids in a prokaryotic host cell under conditions in which inclusion bodies are formed. The antifusogenic peptide recovered from the inclusion bodies is a fragment cleaved from the fusion peptide which comprises an antifusogenic peptide.
摘要翻译：提供了通过在形成包涵体的条件下在原核宿主细胞中产生约14个氨基酸至多70个氨基酸的融合肽来制备抗融合肽的方法。从包涵体回收的抗融合肽是从包含抗融合肽的融合肽切割的片段。

2. 发明申请

US20050058659A1 Process for making antifusogenic fusion peptides that form inclusion bodies 有权
标题翻译：制造形成包涵体的抗融合融合肽的方法
公开(公告)号：US20050058659A1
公开(公告)日：2005-03-17
申请号：US10969624
申请日：2004-10-20
申请人： Eva Hoess , Thomas Meier , Gabriele Pestlin , Friedrich Popp , Klaus Reichert , Rainer Schmuck , Bernd Schneidinger , Christoph Seidel , Wilhelm Tischer
发明人： Eva Hoess , Thomas Meier , Gabriele Pestlin , Friedrich Popp , Klaus Reichert , Rainer Schmuck , Bernd Schneidinger , Christoph Seidel , Wilhelm Tischer
IPC分类号： C12N15/09 , A61K38/00 , A61P31/12 , A61P31/18 , C07K14/155 , C07K14/16 , C12N1/21 , C12N15/62 , C12P21/02 , C12Q1/70 , A61K39/21 , C07H21/04
CPC分类号： C07K14/005 , C07K2319/00 , C12N2740/15022 , C12N2740/16122 , C12P21/02
摘要： A process is disclosed for the production of an antifusogenic peptide by producing a fusion peptide of a length of about 14 to 70 amino acids in a prokaryotic host cell, comprising the steps, under such conditions that inclusion bodies of said fusion peptide are formed, of: (a) expressing in said host cell a nucleic acid encoding said fusion peptide consisting of a first peptide which is an antifusogenic peptide of a length of about 10 to 50 amino acids and a second peptide of a length of about 4 to 30 amino acids, said first peptide being N-terminally linked to said second peptide; (b) cultivating said host cell to produce said inclusion bodies; and (c) recovering said antifusogenic peptide from said inclusion bodies, wherein said recovered antifusogenic peptide consists of said fusion peptide or a peptide comprising the antifusogenic peptide of about 10 to 50 amino acids and which is a fragment cleaved from said fusion peptide. Inclusion bodies of the peptides are disclosed. Also disclosed is a nucleic acid encoding the fusion peptide consisting of a first peptide which is an antifusogenic peptide selected from the group of SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, and said sequences further consisting of glycine at the C terminal end, N-terminally linked to the second peptide.
摘要翻译：公开了通过在原核宿主细胞中产生约14至70个氨基酸长度的融合肽来生产抗融合肽的方法，包括以下步骤：在形成所述融合肽的包涵体的条件下，（a）在所述宿主细胞中表达编码所述融合肽的核酸，所述融合肽由长度为约10至50个氨基酸的抗融合肽的第一肽和长度为约4-30个氨基酸的第二个肽组成所述第一肽与所述第二肽N-末端连接; （b）培养所述宿主细胞以产生所述包涵体; 和（c）从所述包涵体中回收所述抗融合肽，其中所述回收的抗融合肽由所述融合肽或包含约10至50个氨基酸的抗融合肽的肽组成，并且是从所述融合肽切割的片段。公开了肽的包涵体。还公开了编码由第一肽组成的融合肽的核酸，第一肽是选自SEQ ID NO：7，SEQ ID NO：8，SEQ ID NO：9，SEQ ID NO：10的抗融合肽，所述序列进一步由C末端的甘氨酸组成，N-末端与第二肽连接。

3. 发明授权

US07348423B2 Nucleic acids encoding fusion peptides 有权
标题翻译：编码融合肽的核酸
公开(公告)号：US07348423B2
公开(公告)日：2008-03-25
申请号：US10969624
申请日：2004-10-20
申请人： Eva Hoess , Thomas Meier , Gabriele Pestlin , Friedrich Popp , Klaus Reichert , Rainer Schmuck , Bernd Schneidinger , Christoph Seidel , Wilhelm Tischer
发明人： Eva Hoess , Thomas Meier , Gabriele Pestlin , Friedrich Popp , Klaus Reichert , Rainer Schmuck , Bernd Schneidinger , Christoph Seidel , Wilhelm Tischer
IPC分类号： A61K39/21
CPC分类号： C07K14/005 , C07K2319/00 , C12N2740/15022 , C12N2740/16122 , C12P21/02
摘要： A process is disclosed for the production of an antifusogenic peptide by producing a fusion peptide of a length of about 14 to 70 amino acids in a prokaryotic host cell, comprising the steps, under such conditions that inclusion bodies of said fusion peptide are formed, of: (a) expressing in said host cell a nucleic acid encoding said fusion peptide consisting of a first peptide which is an antifusogenic peptide of a length of about 10 to 50 amino acids and a second peptide of a length of about 4 to 30 amino acids, said first peptide being N-terminally linked to said second peptide; (b) cultivating said host cell to produce said inclusion bodies; and (c) recovering said antifusogenic peptide from said inclusion bodies, wherein said recovered antifusogenic peptide consists of said fusion peptide or a peptide comprising the antifusogenic peptide of about 10 to 50 amino acids and which is a fragment cleaved from said fusion peptide. Inclusion bodies of the peptides are disclosed. Also disclosed is a nucleic acid encoding the fusion peptide consisting of a first peptide which is an antifusogenic peptide selected from the group of SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, and said sequences further consisting of glycine at the C terminal end, N-terminally linked to the second peptide.
摘要翻译：公开了通过在原核宿主细胞中产生约14至70个氨基酸长度的融合肽来生产抗融合肽的方法，包括以下步骤：在形成所述融合肽的包涵体的条件下，（a）在所述宿主细胞中表达编码所述融合肽的核酸，所述融合肽由长度为约10至50个氨基酸的抗融合肽的第一肽和长度为约4-30个氨基酸的第二个肽组成所述第一肽与所述第二肽N-末端连接; （b）培养所述宿主细胞以产生所述包涵体; 和（c）从所述包涵体中回收所述抗融合肽，其中所述回收的抗融合肽由所述融合肽或包含约10至50个氨基酸的抗融合肽的肽组成，并且是从所述融合肽切割的片段。公开了肽的包涵体。还公开了编码由第一肽组成的融合肽的核酸，第一肽是选自SEQ ID NO：7，SEQ ID NO：8，SEQ ID NO：9，SEQ ID NO：10的抗融合肽，所述序列进一步由C末端的甘氨酸组成，N-末端与第二肽连接。

4. 发明申请

US20090118476A1 Purification of pegylated polypeptides 有权
标题翻译：聚乙二醇化多肽的纯化
公开(公告)号：US20090118476A1
公开(公告)日：2009-05-07
申请号：US12218545
申请日：2008-07-16
申请人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
发明人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
IPC分类号： C07K1/18
CPC分类号： C07K1/18 , A61K47/60 , C07K14/505
摘要： The invention is a method for the purification of mono-PEGylated erythropoietin using two cation exchange chromatography steps wherein the same type of cation exchange material is used in both cation exchange chromatography steps and a method for producing a mono-PEGylated erythropoietin in substantially homogeneous form.
摘要翻译：本发明是使用两种阳离子交换层析步骤纯化单聚乙二醇化促红细胞生成素的方法，其中在阳离子交换色谱步骤中使用相同类型的阳离子交换材料，以及用于生产基本均匀形式的单PEG化促红细胞生成素的方法。

5. 发明授权

US4564993A Double nut and method of forming the double nut 失效
标题翻译：双螺母和双螺母形成方法
公开(公告)号：US4564993A
公开(公告)日：1986-01-21
申请号：US471193
申请日：1983-03-01
申请人： Gunter Blaurock , Ernst Albert , Werner Blaurock , Benno Peichl , Klaus Reichert , Hans-Georg Stender , Rudolf Schlereth
发明人： Gunter Blaurock , Ernst Albert , Werner Blaurock , Benno Peichl , Klaus Reichert , Hans-Georg Stender , Rudolf Schlereth
IPC分类号： F16H25/22 , B23P11/00
CPC分类号： F16H25/2209 , Y10T29/4984 , Y10T29/49861 , Y10T29/49863 , Y10T29/49885 , Y10T29/49945 , Y10T29/49948 , Y10T29/49959 , Y10T29/49966 , Y10T74/18728
摘要： In a screw drive, in particular a ball screw drive, a double nut, formed of a pair of individual nuts, is tightened on a threaded spindle and the individual nuts are secured together against relative rotation by a locking member. The locking member is secured within a radially extending recess formed in and bridging the individual nuts. The locking member can be inserted with a certain amount of play into the recess or borehole and then secured within the recess by a cement or similar material so that it cannot be displaced. Various types of locking members can be used, such as locking bolts or threaded bolts. When the locking member is connected into the recess or ball it prevents any rotation of the individual nuts relative to one another.
摘要翻译：在螺杆驱动器中，特别是滚珠丝杠驱动器中，由一对独立螺母形成的双螺母被拧紧在螺纹主轴上，并且各个螺母通过锁定构件相对旋转而固定在一起。锁定构件固定在径向延伸的凹部中，该凹槽形成并桥接各个螺母。锁定构件可以以一定量的游隙插入到凹部或钻孔中，然后通过水泥或类似材料固定在凹槽内，使得其不能移位。可以使用各种类型的锁定构件，例如锁定螺栓或螺栓。当锁定构件连接到凹槽或球时，它防止各个螺母相对于彼此的任何旋转。

6. 发明授权

US08138317B2 Purification of pegylated polypeptides 有权
标题翻译：聚乙二醇化多肽的纯化
公开(公告)号：US08138317B2
公开(公告)日：2012-03-20
申请号：US12218545
申请日：2008-07-16
申请人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
发明人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
IPC分类号： C07K1/00 , B01D15/08
CPC分类号： C07K1/18 , A61K47/60 , C07K14/505
摘要： The invention is a method for the purification of mono-PEGylated erythropoietin using two cation exchange chromatography steps wherein the same type of cation exchange material is used in both cation exchange chromatography steps and a method for producing a mono-PEGylated erythropoietin in substantially homogeneous form.
摘要翻译：本发明是使用两种阳离子交换层析步骤纯化单聚乙二醇化促红细胞生成素的方法，其中在阳离子交换色谱步骤中使用相同类型的阳离子交换材料，以及用于生产基本均匀形式的单PEG化促红细胞生成素的方法。

7. 发明授权

US08795533B2 Chromatographic methods 有权
标题翻译：色谱法
公开(公告)号：US08795533B2
公开(公告)日：2014-08-05
申请号：US12925642
申请日：2010-10-26
申请人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
发明人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
IPC分类号： B01D15/20 , B01D15/36 , B01J49/00 , C07K1/18 , C07K14/505
CPC分类号： B01D15/203 , B01D15/362 , B01J49/53 , B01J49/60 , C07K1/18 , C07K14/505
摘要： The current invention comprises a method for the regeneration of a cation exchange chromatography column.
摘要翻译：本发明包括用于再生阳离子交换色谱柱的方法。

8. 发明申请

US20110065903A1 Chromatographic methods 有权
标题翻译：色谱法
公开(公告)号：US20110065903A1
公开(公告)日：2011-03-17
申请号：US12925642
申请日：2010-10-26
申请人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
发明人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
IPC分类号： C07K1/18
CPC分类号： B01D15/203 , B01D15/362 , B01J49/53 , B01J49/60 , C07K1/18 , C07K14/505
摘要： The current invention comprises a method for the regeneration of a cation exchange chromatography column.
摘要翻译：本发明包括用于再生阳离子交换色谱柱的方法。

9. 发明申请

US20090101585A1 Chromatographic methods 有权
标题翻译：色谱法
公开(公告)号：US20090101585A1
公开(公告)日：2009-04-23
申请号：US12218543
申请日：2008-07-16
申请人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
发明人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
IPC分类号： B01D15/20 , C07K14/505
CPC分类号： B01D15/203 , B01D15/362 , B01J49/53 , B01J49/60 , C07K1/18 , C07K14/505
摘要： The current invention comprises a method for the regeneration of a cation exchange chromatography column.
摘要翻译：本发明包括用于再生阳离子交换色谱柱的方法。

10. 发明授权

US08889837B2 Purification of pegylated polypeptides 有权
标题翻译：聚乙二醇化多肽的纯化
公开(公告)号：US08889837B2
公开(公告)日：2014-11-18
申请号：US12931070
申请日：2011-01-24
申请人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
发明人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
IPC分类号： C07K1/14 , C07K1/16 , C07K1/18 , C07K1/22 , C07K14/505 , A61K47/48
CPC分类号： C07K1/18 , A61K47/60 , C07K14/505
摘要： The invention is a method for the purification of mono-PEGylated erythropoietin using two cation exchange chromatography steps wherein the same type of cation exchange material is used in both cation exchange chromatography steps and a method for producing a mono-PEGylated erythropoietin in substantially homogeneous form.
摘要翻译：本发明是使用两种阳离子交换层析步骤纯化单聚乙二醇化促红细胞生成素的方法，其中在阳离子交换色谱步骤中使用相同类型的阳离子交换材料，以及用于生产基本均匀形式的单PEG化促红细胞生成素的方法。

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