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1. 发明申请

US20090269766A1 NUCLEIC ACID AMPLIFICATION IN THE PRESENCE OF MODIFIED RANDOMERS 审中-公开
标题翻译：核素放射治疗存在改良型放射治疗
公开(公告)号：US20090269766A1
公开(公告)日：2009-10-29
申请号：US12406450
申请日：2009-03-18
申请人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
发明人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
IPC分类号： C12Q1/68 , C12N9/00 , C12P19/34
CPC分类号： C12P19/34 , C12Q1/6848 , C12Q1/686 , C12Q2525/179 , C12Q2525/101
摘要： The present invention is directed to a composition comprising a DNA Polymerase which is preferably thermostable, Deoxynucleotides, at least one primer oligonucleotide or a pair of amplification primers, and randomized 5-8 mer oligonucleotide, characterized in that said oligonucleotide comprises a modification with an organic hydrophobic moiety Such a composition is specifically useful for performing hot start PCR.
摘要翻译：本发明涉及包含优选热稳定性的DNA聚合酶，脱氧核苷酸，至少一种引物寡核苷酸或一对扩增引物和随机化的5-8位寡核苷酸的组合物，其特征在于所述寡核苷酸包含与有机疏水部分这样的组合物特别适用于进行热启动PCR。

2. 发明授权

US08119353B2 Rapid one-step reverse transcriptase PCR 有权
标题翻译：快速一步逆转录酶PCR
公开(公告)号：US08119353B2
公开(公告)日：2012-02-21
申请号：US12061700
申请日：2008-04-03
申请人： Waltraud Ankenbauer , Ursula Grepl , Rita Haerteis
发明人： Waltraud Ankenbauer , Ursula Grepl , Rita Haerteis
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6846 , C12Q2527/113 , C12Q2521/107
摘要： The present invention is directed to a method for performing a one-step RT-PCR for amplifying a target RNA comprising the steps (i) providing a sample which is supposed to contain said target RNA (ii) adding a reaction mixture comprising all reagents necessary to reverse transcribe said target RNA into cDNA and amplify at least a portion of said cDNA (iii) incubating said sample for a time interval of 0 seconds to 40 seconds at a temperature between 20° C. and 65° C., and (iv) subjecting said sample to multiple cycles of a thermocycling protocol wherein the temperature of said sample is varied between at least a first temperature between 37° C. and 72° C. and a second temperature between 85° C. and 100° C.
摘要翻译：本发明涉及一种用于进行扩增靶RNA的一步RT-PCR的方法，包括步骤（i）提供假定含有所述靶RNA的样品（ii）加入包含所需试剂的反应混合物将所述靶RNA逆转录入cDNA并扩增所述cDNA的至少一部分（iii）在20℃至65℃的温度下将所述样品温育0秒至40秒的时间间隔，和（iv ）使所述样品经受热循环方案的多个循环，其中所述样品的温度在37℃至72℃的至少第一温度和85℃至100℃的第二温度之间变化。

3. 发明申请

US20100258742A1 DYE COMPOSITION FOR LIQUID TRANSFER CONTROL 有权
标题翻译：用于液体转移控制的染料组合物
公开(公告)号：US20100258742A1
公开(公告)日：2010-10-14
申请号：US12748535
申请日：2010-03-29
申请人： Dieter Heindl , Waltraud Ankenbauer , Hans-Peter Josel , Christian Weilke
发明人： Dieter Heindl , Waltraud Ankenbauer , Hans-Peter Josel , Christian Weilke
IPC分类号： G01N21/64 , C09K11/06
CPC分类号： C12Q1/6818 , C12Q1/6848 , C12Q2565/101 , C12Q2563/131 , C12Q2545/101 , C12Q2537/157 , C12Q2565/501
摘要： The present invention provides kits and methods for composition ratio control based on dyes that are designed to enable energy transfer between each other. In more detail, with the method of the present invention it is possible to verify the mixing ratio of a first component comprising a first dye with a second component comprising a second dye.
摘要翻译：本发明提供了基于设计成能够彼此能量传递的染料的组成比控制的试剂盒和方法。更详细地，利用本发明的方法，可以验证包含第一染料的第一组分与包含第二染料的第二组分的混合比。

4. 发明申请

US20090181401A1 Detection format for hot start real time polymerase chain reaction 审中-公开
标题翻译：检测格式为热启动实时聚合酶链反应
公开(公告)号：US20090181401A1
公开(公告)日：2009-07-16
申请号：US12407339
申请日：2009-03-19
申请人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
发明人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q2561/113 , C12Q2531/113 , C12Q2521/319
摘要： The present invention is directed to a method and a composition for amplifying and detecting a target nucleic comprising subjecting said target nucleic acid to a real time PCR amplification reaction in the presence of a thermostable DNA polymerase, a thermostable double strand dependent 3′-5′ exonuclease having a temperature optimum above 37° C., a pair of amplification primers, deoxynucleoside triphosphates, a detecting oligonucleotide carrying a first label and a second label, said first label being capable of acting as a fluorescent reporter entity when excited with light of an appropriate wavelength, said second label being capable of acting as a fluorescence quenching entity of said fluorescent reporter entity, characterized in that one label is bound to the 3′ end of said detecting oligonucleotide, and further characterized in that the other label is bound either internally or at the 5′ end of said detecting oligonucleotide, and monitoring fluorescence of said fluorescent reporter entity at least after a plurality of amplification cycles.
摘要翻译：本发明涉及用于扩增和检测靶核酸的方法和组合物，其包括在热稳定性DNA聚合酶，耐热双链依赖性3'-5'的存在下使所述靶核酸进行实时PCR扩增反应，温度最高在37℃以上的外切核酸酶，一对扩增引物，脱氧核苷三磷酸，携带第一标记和第二标记的检测寡核苷酸，所述第一标记当用所述第二标记能够用作所述荧光报道实体的荧光猝灭实体，其特征在于一个标记与所述检测寡核苷酸的3'末端结合，其特征还在于另一个标记在内部结合或在所述检测寡核苷酸的5'末端，并监测所述荧光报告的荧光至少在多个放大循环之后。

5. 发明申请

US20050037410A1 Detection format for hot start real time polymerase chain reaction 审中-公开
标题翻译：检测格式为热启动实时聚合酶链反应
公开(公告)号：US20050037410A1
公开(公告)日：2005-02-17
申请号：US10903992
申请日：2004-07-30
申请人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
发明人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
IPC分类号： G01N33/58 , C12N15/09 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6818 , C12Q2561/113 , C12Q2531/113 , C12Q2521/319
摘要： The present invention is directed to a method and a composition for amplifying and detecting a target nucleic comprising subjecting said target nucleic acid to a real time PCR amplification reaction in the presence of a thermostable DNA polymerase, a thermostable double strand dependent 3′-5′ exonuclease having a temperature optimum above 37° C., a pair of amplification primers, deoxynucleoside triphosphates, a detecting oligonucleotide carrying a first label and a second label, said first label being capable of acting as a fluorescent reporter entity when excited with light of an appropriate wavelength, said second label being capable of acting as a fluorescence quenching entity of said fluorescent reporter entity, characterized in that one label is bound to the 3′ end of said detecting oligonucleotide, and further characterized in that the other label is bound either internally or at the 5′ end of said detecting oligonucleotide, and monitoring fluorescence of said fluorescent reporter entity at least after a plurality of amplification cycles.
摘要翻译：本发明涉及用于扩增和检测靶核酸的方法和组合物，其包括在热稳定性DNA聚合酶，耐热双链依赖性3'-5'的存在下使所述靶核酸进行实时PCR扩增反应，温度最高在37℃以上的外切核酸酶，一对扩增引物，脱氧核苷三磷酸，携带第一标记和第二标记的检测寡核苷酸，所述第一标记当用所述第二标记能够用作所述荧光报道实体的荧光猝灭实体，其特征在于一个标记与所述检测寡核苷酸的3'末端结合，其特征还在于另一个标记在内部结合或在所述检测寡核苷酸的5'末端，并监测所述荧光报告的荧光至少在多个放大循环之后。

6. 发明授权

US08674080B2 Dye composition for liquid transfer control 有权
标题翻译：用于液体转移控制的染料组合物
公开(公告)号：US08674080B2
公开(公告)日：2014-03-18
申请号：US12748535
申请日：2010-03-29
申请人： Dieter Heindl , Waltraud Ankenbauer , Hans-Peter Josel , Christian Weilke
发明人： Dieter Heindl , Waltraud Ankenbauer , Hans-Peter Josel , Christian Weilke
IPC分类号： C07H21/04 , G01N21/62
CPC分类号： C12Q1/6818 , C12Q1/6848 , C12Q2565/101 , C12Q2563/131 , C12Q2545/101 , C12Q2537/157 , C12Q2565/501
摘要： The present invention provides kits and methods for composition ratio control based on dyes that are designed to enable energy transfer between each other. In more detail, with the method of the present invention it is possible to verify the mixing ratio of a first component comprising a first dye with a second component comprising a second dye.
摘要翻译：本发明提供了基于设计成能够彼此能量传递的染料的组成比控制的试剂盒和方法。更详细地，利用本发明的方法，可以验证包含第一染料的第一组分与包含第二染料的第二组分的混合比。

7. 发明申请

US20130109060A1 Nucleic Acid Amplification in the Presence of Modified Randomers 失效
标题翻译：核酸扩增在修饰的兰姆斯存在下
公开(公告)号：US20130109060A1
公开(公告)日：2013-05-02
申请号：US13711846
申请日：2012-12-12
申请人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
发明人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12Q1/6848 , C12Q1/686 , C12Q2525/179 , C12Q2525/101
摘要： The present invention is directed to a composition comprising a DNA Polymerase which is preferably thermostable, Deoxynucleotides, at least one primer oligonucleotide or a pair of amplification primers, and randomized 5-8 mer oligonucleotide, characterized in that said oligonucleotide comprises a modification with an organic hydrophobic moiety Such a composition is specifically useful for performing hot start PCR.
摘要翻译：本发明涉及包含优选热稳定性的DNA聚合酶，脱氧核苷酸，至少一种引物寡核苷酸或一对扩增引物和随机化的5-8位寡核苷酸的组合物，其特征在于所述寡核苷酸包含与有机疏水部分这样的组合物特别适用于进行热启动PCR。

8. 发明授权

US07910720B2 Polyanion for improved nucleic acid amplification 有权
标题翻译：用于改善核酸扩增的聚阴离子
公开(公告)号：US07910720B2
公开(公告)日：2011-03-22
申请号：US12547013
申请日：2009-08-25
申请人： Waltraud Ankenbauer , Dieter Heindl , Frank Laue , Eva Walter , Renate Kolb
发明人： Waltraud Ankenbauer , Dieter Heindl , Frank Laue , Eva Walter , Renate Kolb
IPC分类号： C07H21/04 , C12P19/34 , C12Q1/68 , B01L3/00
CPC分类号： C07H21/00 , C12Q1/6848 , C12Q2527/125
摘要： The present invention is directed to a novel chemical compound comprising the structure [Xx-(CH2)m-phosphate-Yy]n, characterized in that 3≦m≦6, 30≦n≦60, each x and y is independently from each other 0 or 1, each X and Y is independently from each other any photometrically measurable entity; provided that the terminal X can also be an —OH group or a phosphate group, and further provided that the terminal Y can also be an —OH group. Such a compound can be used as a suitable hot start additive for PCR based amplification of nucleic acids.
摘要翻译：本发明涉及一种包含结构[Xx-（CH2）m-磷酸-Yy] n结构的新化合物，其特征在于，3和nlE; m＆nlE; 6,30＆lt; n; n＆nlE; 60，每个x和y各自独立地表示其他0或1，每个X和Y彼此独立地具有任何光度测量实体; 条件是末端X也可以是-OH基或磷酸基，并且进一步规定，末端Y也可以是-OH基。这种化合物可以用作基于PCR扩增核酸的合适的热启动添加剂。

9. 发明授权

US07425423B1 Thermostable nucleic acid polymerase from Thermococcus gorgonarius 失效
标题翻译：来自Thermococcus gorgonarius的热稳定性核酸聚合酶
公开(公告)号：US07425423B1
公开(公告)日：2008-09-16
申请号：US09269860
申请日：1997-10-01
申请人： Waltraud Ankenbauer , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
发明人： Waltraud Ankenbauer , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
IPC分类号： C12N9/12 , C12Q1/48
CPC分类号： C12N9/1252
摘要： A purified thermostable enzyme is derived from the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3′-5′ proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).
摘要翻译：纯化的热稳定酶源自嗜热古细菌热球菌。该酶可以是天然的或重组的，在稳定剂存在下在95℃孵育2小时后保留其活性的约90％，并且具有3'-5'校对外切核酸酶活性。热稳定的DNA聚合酶可用于许多重组DNA技术，特别是通过聚合酶链反应（PCR）进行的核酸扩增。

10. 发明授权

US07122355B2 Composition and method for hot start nucleic acid amplification 有权
公开(公告)号：US07122355B2
公开(公告)日：2006-10-17
申请号：US10192902
申请日：2002-07-11
申请人： Waltraud Ankenbauer , Dieter Heindl , Frank Laue , Andreas Huber
发明人： Waltraud Ankenbauer , Dieter Heindl , Frank Laue , Andreas Huber
IPC分类号： C12P19/34
CPC分类号： C12Q1/6848 , C12Q1/6853 , C12Q2549/101 , C12Q2525/186 , C12Q2521/319
摘要： The present invention is directed to a new composition for performing a nucleic acid amplification reaction comprising (i) a thermostable DNA-Polymerase, (ii) a thermostable 3′-5′ Exonuclease, and (iii) at least one primer for nucleic acid amplification with a modified 3′ terminal residue which is not elongated by said thermostable DNA-Polymerase as well as methods for performing a PCR reaction using this composition. Furthermore, the method is directed to kits comprising such a composition.

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