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1. 发明授权

US5962237A Method of enriching rare cells 失效
标题翻译：丰富稀有细胞的方法
公开(公告)号：US5962237A
公开(公告)日：1999-10-05
申请号：US832468
申请日：1997-04-02
申请人： Paul O. P. Ts'o , Zheng-Pin Wang , Stephen A. Lesko , William G. Nelson , Alan W. Partin
发明人： Paul O. P. Ts'o , Zheng-Pin Wang , Stephen A. Lesko , William G. Nelson , Alan W. Partin
IPC分类号： C12N15/09 , C12N5/06 , C12N5/08 , C12N5/10 , C12Q1/68 , G01N33/48 , G01N33/569 , G01N33/574 , B01D33/15 , G01N9/30 , G01N33/53
CPC分类号： G01N33/57407 , G01N33/56966 , G01N33/57434 , G01N33/57438 , G01N33/57488
摘要： A method for enriching cancer cells such as prostate cancer cells in a bodily fluid sample is disclosed comprising (a) obtaining the sample comprising cancer cells and non-rare cells; (b) subjecting the sample to multiple density gradient separation comprising a first density gradient and a second density gradient, wherein the second density gradient is greater than the first density gradient, and producing a first fluid comprising an increased concentration of cancer cells of a first density, and a second fluid comprising an increased concentration of cancer cells of a second density, wherein the second density is greater than the first density; wherein subjecting the sample to multiple density gradient separation includes producing a plasma layer, a first interface layer, a first gradient layer, a second interface layer, a second gradient layer, and a cell pellet; wherein producing the first fluid includes combining the first interface layer and the first gradient layer and forming a first suspension; and wherein producing the second fluid includes combining the second interface layer and the second gradient layer and forming a second suspension; (c) subjecting said second fluid comprising the second suspension to a binding agent that binds non-rare cells; and (d) removing the bound non-rare cells from the second fluid to provide a second fluid enriched with the greater density cancer cells.
摘要翻译：公开了一种在体液样品中富集癌细胞如前列腺癌细胞的方法，其包括（a）获得包含癌细胞和非罕见细胞的样品; （b）对样品进行多密度梯度分离，其包括第一密度梯度和第二密度梯度，其中所述第二密度梯度大于所述第一密度梯度，并且产生第一流体，所述第一流体包含增加浓度的第一并且包括第二密度增加的癌细胞浓度的第二流体，其中所述第二密度大于所述第一密度; 其中对样品进行多重密度梯度分离包括产生等离子体层，第一界面层，第一梯度层，第二界面层，第二梯度层和细胞沉淀物; 其中制备所述第一流体包括组合所述第一界面层和所述第一梯度层并形成第一悬浮液; 并且其中制备所述第二流体包括组合所述第二界面层和所述第二梯度层并形成第二悬浮液; （c）使包含第二悬浮液的所述第二流体接合结合非稀有细胞的结合剂; 和（d）从第二流体中除去结合的非稀有细胞以提供富含更大密度的癌细胞的第二种液体。

2. 发明授权

US06960449B2 Class characterization of circulating cancer cells isolated from body fluids and methods of use 有权
标题翻译：从体液分离的循环癌细胞的分类表征和使用方法
公开(公告)号：US06960449B2
公开(公告)日：2005-11-01
申请号：US09501179
申请日：2000-02-10
申请人： Zheng-Pin Wang , Paul O. P. Ts'o
发明人： Zheng-Pin Wang , Paul O. P. Ts'o
IPC分类号： G01N33/50 , G01N33/574 , G01N1/30 , G01N33/49
CPC分类号： G01N33/57496 , G01N33/5091
摘要： The present invention relates to the identification and characterization of classes and subclasses of circulating cancer cells, including microtumors from body fluid samples using molecular, cytological, and morphological analyses, and methods for staging patients and measuring the efficacy of medical treatments.
摘要翻译：本发明涉及循环癌细胞的类别和亚类的鉴定和表征，包括使用分子，细胞学和形态学分析的体液样品的微量肿瘤，以及用于分期患者和测量药物疗效的方法。

3. 发明申请

US20060228339A1 Methods of preparing transplantable product for treatment of skin defects 审中-公开
标题翻译：制备可移植产品以治疗皮肤缺陷的方法
公开(公告)号：US20060228339A1
公开(公告)日：2006-10-12
申请号：US10564740
申请日：2003-08-22
申请人： Zheng-Pin Wang
发明人： Zheng-Pin Wang
IPC分类号： A61K35/30
CPC分类号： C12N5/0629 , A61K35/36 , A61L27/3604 , A61L27/60 , C12N5/0632 , C12N5/0682 , C12N5/0698 , C12N2502/094 , C12N2502/1323 , C12N2533/92
摘要： A method for preparing a tissue culture insert that is used for constructing a transplantable graft of an engineered tissue equivalent comprising living main functional cells, stromal cells and tissue matrix on/in a biological supporting membrane for treatment of body tissue defects.
摘要翻译：一种制备组织培养插入物的方法，所述组织培养插入物用于构建工程组织等同物的可移植移植物，其包含生物支持膜上的生物主功能细胞，基质细胞和组织基质，用于治疗身体组织缺陷。

4. 发明申请

US20050175623A1 Saponins as anticancer agent 审中-公开
标题翻译：皂苷作为抗癌剂
公开(公告)号：US20050175623A1
公开(公告)日：2005-08-11
申请号：US10775632
申请日：2004-02-10
申请人： Zheng-Pin Wang
发明人： Zheng-Pin Wang
IPC分类号： A61K31/704 , C07J17/00
CPC分类号： C07J17/00 , A61K31/704 , A61K2300/00
摘要： The invention described herein encompasses novel discovery of anticancer agents and its compositions comprising saponins, a group of triterpenoid and steroidal saponins found in plants including Quillaja saponaria Molina (soap tree), which are used as therapeutic compounds for the treatment and prevention of cancer diseases or as a dietary supplement that offers tumor cell killing and tumor cell inhibition, and also as a anticancer potentiator with other anticancer agents.
摘要翻译：本文描述的发明包括抗癌剂及其组合物的新发现，所述抗癌剂及其组合物包含在包括Quillaja saponaria Molina（肥皂树）的植物中发现的皂角苷，一类三萜类化合物和类固醇皂苷，其用作治疗和预防癌症疾病的治疗化合物或作为提供肿瘤细胞杀伤和肿瘤细胞抑制的膳食补充剂，以及作为其他抗癌剂的抗癌增强剂。

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