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    • 5. 发明授权
    • Method of enriching rare cells
    • 丰富稀有细胞的方法
    • US5962237A
    • 1999-10-05
    • US832468
    • 1997-04-02
    • Paul O. P. Ts'oZheng-Pin WangStephen A. LeskoWilliam G. NelsonAlan W. Partin
    • Paul O. P. Ts'oZheng-Pin WangStephen A. LeskoWilliam G. NelsonAlan W. Partin
    • C12N15/09C12N5/06C12N5/08C12N5/10C12Q1/68G01N33/48G01N33/569G01N33/574B01D33/15G01N9/30G01N33/53
    • G01N33/57407G01N33/56966G01N33/57434G01N33/57438G01N33/57488
    • A method for enriching cancer cells such as prostate cancer cells in a bodily fluid sample is disclosed comprising (a) obtaining the sample comprising cancer cells and non-rare cells; (b) subjecting the sample to multiple density gradient separation comprising a first density gradient and a second density gradient, wherein the second density gradient is greater than the first density gradient, and producing a first fluid comprising an increased concentration of cancer cells of a first density, and a second fluid comprising an increased concentration of cancer cells of a second density, wherein the second density is greater than the first density; wherein subjecting the sample to multiple density gradient separation includes producing a plasma layer, a first interface layer, a first gradient layer, a second interface layer, a second gradient layer, and a cell pellet; wherein producing the first fluid includes combining the first interface layer and the first gradient layer and forming a first suspension; and wherein producing the second fluid includes combining the second interface layer and the second gradient layer and forming a second suspension; (c) subjecting said second fluid comprising the second suspension to a binding agent that binds non-rare cells; and (d) removing the bound non-rare cells from the second fluid to provide a second fluid enriched with the greater density cancer cells.
    • 公开了一种在体液样品中富集癌细胞如前列腺癌细胞的方法,其包括(a)获得包含癌细胞和非罕见细胞的样品; (b)对样品进行多密度梯度分离,其包括第一密度梯度和第二密度梯度,其中所述第二密度梯度大于所述第一密度梯度,并且产生第一流体,所述第一流体包含增加浓度的第一 并且包括第二密度增加的癌细胞浓度的第二流体,其中所述第二密度大于所述第一密度; 其中对样品进行多重密度梯度分离包括产生等离子体层,第一界面层,第一梯度层,第二界面层,第二梯度层和细胞沉淀物; 其中制备所述第一流体包括组合所述第一界面层和所述第一梯度层并形成第一悬浮液; 并且其中制备所述第二流体包括组合所述第二界面层和所述第二梯度层并形成第二悬浮液; (c)使包含第二悬浮液的所述第二流体接合结合非稀有细胞的结合剂; 和(d)从第二流体中除去结合的非稀有细胞以提供富含更大密度的癌细胞的第二种液体。